Differentially-expressed conifer cDNAs, and their use in improving somatic embryogenesis

ABSTRACT

The invention relates to a method for staging embryos of plants. In particular, this invention relates to a method for creating a relational database by determining transcript levels of sets of genes expressed at predetermined stages in embryo development. This approach creates a method by which embryos of unknown stage development can be determined by comparisons between expression levels of those embryos to the expression levels found in the database. This approach further allows rapid identification of transcripts in an embryo to be staged by the utilization of probes corresponding to cDNAs comprising the database. Additionally, this invention relates to a method for selecting advantageous plant clones for future propagation. Specifically, this method relates to an approach to link the biochemical condition of an embryo to current culture conditions and thus provides a method for enhancing conditions to produce embryos with a desired biochemical state.

CROSS-REFERENCE TO RELATED APPLICATIONS

This patent application claims benefit of priority of provisionalapplication U.S. Ser. No. 60/239,250, filed Oct. 11, 2000, and claimsbenefit of priority of provisional application U.S. Ser. No. 60/260,882,filed Jan. 12, 2001.

FIELD OF THE INVENTION

The present invention relates to a relational database of cDNAmolecules, including those corresponding to Loblolly Pine MajorIntrinsic Protein (MIP), which are differentially expressed during plantembryogenesis. The present invention further relates to the use of DNAarrays for evaluating gene expression in somatic and zygotic embryos.The invention encompasses related nucleic acids, proteins, antigens, andantibodies derived from these cDNAs as well as the use of such moleculesfor the staging, characterization, and manipulation of plantembryogenesis, in particular conifer embryogenesis. The cDNAs andrelated nucleic acids, proteins, antigens, and antibodies derived fromthese cDNAs are useful in the design, selection, and cultivation ofimproved crops, specifically including coniferous trees, which provideraw materials for paper and wood products.

BACKGROUND OF THE INVENTION

The world demand for paper is expected to increase nearly 50% by theyear 2010 (McNutt and Rennel, Pulp Paper Intern 39: 48 (1997)). TheUnited States' forest products industry faces a great challenge in orderto keep pace with the growing demand for paper. This challenge is madegreater by the decreasing availability of a forest land-base, resultingfrom environmental restrictions and urban growth, from which to harvesttimber resources. Additionally, valuable wood resources are lost to theenvironmental stresses and biotic diseases. Consequently, the push tosecure a renewable and sustainable source of raw material for paper andother wood related products has become an important priority for theforest products industry.

Current forestry related research and development is focused on creatingsustainable fiber farms or tree plantations. Farming trees with elitegermplasms will increase growth rates and yields of wood per acre.However, creating improved tree stock requires the ability to identifyand generate genetically superior trees and a way to propagate suchsuperior trees without diluting their genetic quotient.

A. Breeding and Selection

Addressing the need to propagate genetically superior trees withoutgenetic diminution demands full research attention. Traditional methodsof tree propagation relied on selected breeding programs to achievegenetic gain, i.e., the development of a strain, sub-strain, or linehaving any heritable and economically valuable characteristic orcombination of characteristics not found in the parents. Based on theresults of progeny tests, superior maternal trees are selected and usedin “seed orchards” for mass production of genetically improved seed. Thegenetic gain in such an open-pollinated sexual propagation strategy is,however, limited by the breeder's inability to control the paternalparent. Additional gains can also be achieved by control-pollination ofthe maternal tree with pollen from individual trees whose progeny havedemonstrated superior growth characteristics. Nevertheless, even undercontrolled conditions where both parents of each seed are the same,sexual propagation results in a “family” of seeds, i.e., siblings,comprised of many different genetic combinations. As not all genotypecombinations are favorable, the genetic gain in any particular progenyis frequently offset and obscured by the genetic variation among siblingseeds and those seedlings retaining undesirable or previously maskedpre-cross traits.

In addition to inherent genetic limitations of a traditional breedingprograms, large-scale production of control pollinated seeds is alsoexpensive. Consequently, economic and biological limitations oflarge-scale seed production has lead the industry to turn towardsmethods of asexual reproduction, such as grafting, vegetativepropagation and micropropagation, as more viable alternatives.

B. Asexual (Clonal) Propagation

Asexual propagation permits the application of very high selectionintensity, resulting in the propagation of only those progeny showing ahigh genetic gain potential. These highly desirable progeny can haveunique genetic combinations that result in superior growth andperformance characteristics. Thus, with asexual propagation it ispossible to genetically select individuals while avoiding a concomitantreduction of genetic gain due to intra-familial variation.

Asexual propagation of trees can be accomplished currently by grafting,vegetative propagation, and micropropagation. Grafting, widely used topropagate select individuals in limited quantities for seed orchardestablishment, is not applicable to large-scale production forreforestation. Vegetative propagation, achieved by the rooting ofcuttings, and micropropagation by somatic embryogenesis, currently holdthe most potential for reforestation of conifers. Although vegetativepropagation by rooted cuttings can be achieved in many coniferousspecies, large-scale production via this method is extremely costly dueto difficulties in automating and mechanizing the process, not tomention the need for tremendous quantities of stock tissue. Thispropagation method is still further limited by the fact that the rootingpotential of stock plants decrease with time, making it difficult toserially propagate from select genotypes over extended periods of time.

Micropropagation is the most promising method of asexual propagation formass plantings. This process involves the production of somatic embryosin vitro from minute pieces of plant tissue or individual cells. Theembryos are referred to as somatic because they are derived from thesomatic (vegetative) tissue, rather than from the sexual process. Bothvegetative propagation and micropropagation have the potential tocapture all genetic gain of highly desirable genotypes. However, unlikeconventional vegetative propagation methods, somatic embryogenesis isamenable to automation and mechanization, making it highly desirable forlarge-scale production of planting stock for reforestation. Moreover,somatic embryogenesis is particularly amenable to high intensityselection of a large number of clones. These advantages are compoundedby the ability to safely preserve somatic embryogenic cultures in liquidnitrogen for long-term storage. Consequently, long-term cryogenicpreservation offers immense advantages over other vegetative propagationsystems that attempt to maintain the juvenility of stock plants.Techniques for somatic embryogenesis in a wide variety of plant speciesare well known in the art; exemplary methods for performing somaticembryogenesis in conifers are taught in U.S. Pat. Nos.: 5,036,007;5,236,841; 5,294,549; 5,413,930; 5,491,090; 5,506,136; 5,563,061;5,677,185; 5,731,203; 5,731,204; and 5, 856,191, herein incorporated byreference in their entirety.

Thus, somatic embryogenesis has great potential for clonal production ofconifer embryos to meet the increased demands of the pulp and paperindustry. Assessment of embryo quality, however, needs improvement. Theprocess of creating better tree stock begins with understanding theprocess of tree development from embryogenesis through full maturation.

In general, plant tissue culture is the broad science of growing planttissues on or in a nutrient medium containing minerals, sugars, vitaminsand plant hormones. By adjusting the composition of the media, culturedtissues can be induced to grow or differentiate into specific cell typesor organs. “Somatic embryogenesis” is a type of plant tissue culturewhere a piece of a donor plant is excised, cultured and induced to formmultiple embryos. An embryo is a discrete mass of cells with awell-defined structure that is capable of growing into a whole plant.

The methods generally in use for somatic embryogenesis today involveseveral steps. Prior to the tissue culture process, a suitable “explant”is harvested. A typical explant in conifer somatic embryogenesis is the“megagametophyte”, a haploid nutritive tissue of the conifer seed, whichis extracted from the ovule of a pollinated female cone. This ovulecontains single or multiple zygotic seed embryos. In the seeds of manyconiferous species, one or more genetically unique embryos naturallyundergo a process called cleavage polyembryony, where a zygotic embryogrows and divides to form a small clones of embryos.

The first step in somatic embryogenesis is the initiation step. Theexplant is placed on a suitable media. When the explant is an ovule, aprocess called extrusion occurs. Extrusion involves the emergence orexpulsion of a zygotic embryo or multiple embryos and embryogenic tissueout of the megagametophyte. If culture conditions are suitable,initiation proceeds and the extruded embryo or embryos undergo theprocess of cleavage polyembryony. This results in the formation of earlystage somatic embryos in a glossy, mucilaginous mass.

After embryogenic cultures are initiated, the somatic embryos aretransferred to a second medium with an appropriate composition of planthormones and other factors to induce the somatic embryos to multiply. Inthe multiplication stage, cultures can double up to 2-6 times in oneweek. Once large numbers of embryos are obtained in the multiplicationstage, the embryos are moved to a development and maturation medium.Here, the correct balance of plant hormones and other factors willinduce the early-stage embryos to mature into late stage embryos.Following the maturation and development stage, embryos are germinatedto form small seedlings. These seedlings are then acclimated forsurvival outside of the culture vessel. After acclimation, the seedlingsare ready for planting.

The relative ability to propagate plants by somatic embryogenesis canvary greatly between species. Among conifers, for example, spruce(Picea) species and Douglas fir are easily propagated, while Pinusspecies are much more difficult. Many Pinus species, including Loblollypine (Pinus taeda), do not readily initiate embryonic cultures. Typicalinitiation frequencies between 1% and 12% are reported for various Pinusspecies (Becwar et al., For. Sci. p1-18 (1988), Jain et al., Plant Sci.65:233-241 (1989), Becwar et al., Can. J. For. Res. 20:810 (1990), Liand Huang, J. Tissue Cult. Assoc. 32:129 (1996)). Laine and David,(Plant Sci. 69:215 (1990)), however, were able to obtain highfrequencies of initiation (up to 59%) in Pinus caibaea, suggesting thatnot all Pinus species are recalcitrant. Also, one earlier reportdescribed initiation frequencies of 54% in White pine (Pinus strobus).Finer et al., Plant Cell Rep. 8:203 (1989). However, other workers werenot able to duplicate this success. Michler et al., Plant Sci. 77:111(1991). The results in the literature demonstrate the recalcitrance ofPinus species, especially Loblolly pine, in regeneration by somaticembryogenesis.

Nevertheless, once this process is understood from the standpoint ofdevelopmental genetics, breeders will then have the appropriate tools tomonitor, intervene, and improve both the regeneration frequency and theoverall quality of tree stock through genetic engineering. For example,both environmental requirements and responsiveness of a developingembryo change as the embryo passes various developmental milestones.Consequently, accurate and timely knowledge of the developmental stageof an embryonic culture would allow the skilled practitioner tobeneficially adjust the growth media components and other environmentalfactors to achieve optimal embryo survival, growth, and maturation. Inaddition, an understanding of developmentally regulated genes wouldallow for early selection of advantageous clones and provide tools fordevelopmentally regulated transgenic expression systems.

Currently, a reasonable determination of the precise developmental stageof an embryo requires a practiced, physical familiarity with themorphological appearance of embryos at different stages, which isfurther complicated by the presence of morphological variations betweenspecies. Consequently, visual determination is performed best by expertsin the field. Thus, there is a need in the art for a staging methodwhich can be reliably practiced by the ordinary practitioner. Thecurrent invention will allow one to stage embryos based on a relationaldatabase system profiling gene expression patterns instead of physicalmorphological differences, thereby permitting one less skilled in theart of visual staging to biologically determine the stages ofembryogenesis.

The traditional morphological staging method provides only a crudeindication of the underlying biochemical condition or state of anembryo. This level of information is insufficient for refining cultureconditions, including media formulations, or for selecting potentiallyadvantageous embryo clones for further development. Thus, there is aneed in the art for a more sensitive staging method that preciselydefines the physiological age, health, growth requirements, andpotential fitness of a particular embryo. The current invention willallow definitive staging significantly beyond that currently practicedin the art, and provides a detailed analysis of the biochemical stateand potential fitness of an embryo by comparison to developed relationaldatabase profiles.

Visual staging methods depend on morphological markers to assign anumerical stage of 1-9 to an embryo. Nevertheless, it is well acceptedthat visually undetectable developmental changes occur in an embryoafter it reaches stage 9. The current invention is particularly usefulin providing means for monitoring and evaluating the developmental stateof these older embryos, as genetic responses occur and are detectable upto and through an adult tree's life.

There further exists in the art a need for information regarding theproteins, genes, and gene expression patterns in plant embryodevelopment, as well as a more thorough understanding of how thisinformation relates to the physiology, developmental potential, andgenetic quotient of a plant embryo. The relational database systemprovides a platform for which to monitor individual gene expressionlevels during embryo development while directly correlating expressionwith, for example, environmental conditions, age, and embryo fitness, aswell as the protein identification achieved by BLAST searches ofpublicly available databases (i.e., GenBank) for desirable genes.Accordingly, the present invention therefore provides the additionalability to correlate the direct, global gene expression response withinthe embryo system to a typically non-expressing gene driven by astage-specific promoter.

SUMMARY OF THE INVENTION

The present invention addresses these needs by providing in a relationaldatabase format nucleic acid and protein sequences that aredifferentially expressed during various stages of plant embryogenesis.The invention encompasses a set of isolated nucleic acid moleculescomprising the DNA sequence of any one of SEQ ID NOS: 1-334 and nucleicacid molecules related or complementary to any one of SEQ ID NOS: 1-334.(See Table I) As such, the invention includes both single-stranded anddouble-stranded RNA and DNA nucleic acids, including variants thereof.The nucleic acids of the invention can be used as an expression templatein the form of DNA arrays, including for example, gene arrays, DNAchips, and dot array Southerns, for which to compare and evaluateexpression in test samples. (See Table II) The nucleic acids of theinvention can be further used as probes to detect the presence or levelof both single-stranded and double-stranded RNA and DNA encodingvariants of polypeptides or fragments of polypeptides encompassed by theinvention. The nucleic acids of the invention can be further used aspromoters for the expression of sense and antisense molecules atspecific stages of embryo development. Data acquired through the use ofthe present invention can in turn be provided to the relational databasefor further development.

Isolated nucleic acid molecules that hybridize to a denatured,double-stranded DNA comprising the DNA sequence of any one of SEQ IDNOS: 1-334 under conditions of moderate or high stringency are alsoencompassed by the invention. The invention further encompassessynthetic and naturally-occurring variants of the nucleic acidsdescribed in SEQ ID NOS: 1-334, for example, isolated nucleic acidmolecules derived by in vitro mutagenesis from SEQ ID NOS: 1-334. Invitro mutagenesis would include numerous techniques known in the artincluding, but not limited to, site-directed mutagenesis, randommutagenesis, and in vitro nucleic acid synthesis.

The invention also encompasses related molecules (variants) includingisolated nucleic acid molecules degenerate from SEQ ID NOS: 1-334 as aresult of the genetic code, for example, naturally-occurring orsynthetic allelic variants of the genes encoding SEQ ID NOS: 1-334. Suchrelated molecules also encompass both smaller and larger nucleic acidsthat contain sufficient sequence to support hybridization to any of SEQID NOS: 1-334 under conditions of moderate or high stringency.Consequently, recombinant vectors, including those that direct theexpression of these nucleic acid molecules and host cells transformed ortransfected with these vectors are herein defined as variants and areencompassed by the invention.

Another embodiment of this invention is the production of transgenicvectors and transgenic plants comprising vectors or other nucleic acidscomprising any one of SEQ ID NOS: 1-334 and related molecules.Particularly preferred are those capable of expressing polypeptides orpeptides encoded by any of SEQ ID NOS: 1-327. In a preferred embodiment,the transgene comprises SEQ ID NO: 327, or a variant thereof.

SEQ ID NO: 327 encodes a protein which corresponds to a novel Loblollypine homolog of the plant Major Intrinsic Protein (MIP) family. MIPscomprise a large family of related proteins that function as membranechannels for the transport of water and possibly ions across cellularmembranes. Henceforth, the encoded protein of SEQ ID NO: 327 may bereferred to as Loblolly MIP. Variants, including naturally-occurring andartifactually-programmed allelic variants, vectors, and other nucleicacids which hybridize to SEQ ID NO: 327 under conditions of moderate orhigh stringency are encompassed by the invention. Also encompassed areplant cells, seeds, embryos and trees, transgenic for loblolly pine MIP,and variants thereof.

The invention also encompasses isolated polypeptides, or fragmentsthereof, encoded by any one of the nucleic acid molecules of SEQ ID NOS:1-327, including variants thereof. The invention further encompasses theuse of these peptide sequences as markers for staging, monitoring, andselecting embryos and embryo cultures. The invention also encompassesmethods for the production of these polypeptides or fragments thereofincluding culturing a host cell under conditions promoting expressionand recovering the polypeptide or peptide from the culture medium. Inparticular, the expression of polypeptides or peptides encoded by SEQ IDNOS: 1-327 in viral vectors, bacteria, yeast, plant, and animal cells isencompassed by the invention. Isolated polyclonal or monoclonalantibodies that bind to peptides encoded by SEQ ID NOS: 1-327 are alsoencompassed by the invention.

Further encompassed by this invention are methods for using the nucleicacid molecules of any one of SEQ ID NOS: 1-327 to obtain full lengthcDNA and genomic sequences of the corresponding genes, includingcognate, homologous, or otherwise related genetic sequences, whichhybridize to any of SEQ ID NOS: 1-327 under conditions of moderate orhigh stringency. Also provided by this invention are oligonucleotidesderived from any one of SEQ ID NOS: 1-334 that can be used as probesand/or as primers in PCR, RT-PCR, and other assays to detect thepresence or level of the nucleic acids of SEQ ID NOS: 1-334 and relatedmolecules.

The primers and other probes of the invention may be used to monitor andcharacterize the development of plant embryos, in particular, pine treeembryos. Characterization of embryonic gene expression provides meansfor correlating gene expression with current and potential plantphenotypes. Consequently, the present invention encompasses means formonitoring and adjusting growth conditions (see FIG. 6), as well asmeans for selecting genetically superior embryonic clones for furtherpropagation and expansion (see FIG. 8). Thus, the present inventionencompasses the use of DNA or RNA probes derived from the nucleic acidmolecules of SEQ ID NOS: 1-334 in any form, e.g., in DNA arrays, andantibodies raised against polypeptides or peptide fragments encoded bySEQ ID NOS: 1-327, to determine relative or absolute levels ofexpression of the genes or proteins encoded by SEQ ID NOS: 1-327. Inaddition, these nucleic acid and antibody probes may be used forstaging, monitoring, characterizing, or selecting plant embryos orembryo cultures, particularly pine tree embryos.

The relational database of the present invention allows expressioninformation pertaining to embryo stages to be viewed as sequence datagenerated in accordance with the present invention. The inventionincludes a database for storing a plurality of sequence records forwhich to correlate embryo stages to sequence records. The method furtherinvolves providing an interface which allows a user to select one ormore expression categories contained within the database.

The relational database is designed to include separate parts or cellsfor information storage. One cell or part may contain a gene expressiondatabase which contains nucleic acid molecules of SEQ ID NOS: 1-327.Other cells or parts may contain descriptive information pertaining toeach nucleic acid molecules of SEQ ID NOS: 1-327, additional sequencedata related to the gene expression database, protein encoded by nucleicacids disclosed herein, similarity values to known proteins of othersystems, and to conditions under which expression data was obtained.

The database system described in the present invention will allowidentification or selection of particular genes of interest for furtheruse with DNA arrays. Identification or selection of particular genes mayinclude, for example, those related to patterns of expression, thoseidentified with homology to known genes from other studies, and thosesequences considered novel.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 depicts differential display of loblolly pine zygotic and somaticembryos at different stages of development.

FIG. 2 displays embryo gene expression observed by high-density arraySouthern hybridization.

FIG. 3 provides a general schematic for gene regulation studies arisingfrom the cDNA cloning of genes expressed in embryos.

FIG. 4 depicts graphical representation of hybridization of ‘dehydrin’and LPZ216 cDNA probes to total RNA isolated from zygotic embryos ofloblolly pine.

FIG. 5 displays ABA concentration of loblolly pine embryos.

FIG. 6 shows schematic of gene study for improved somatic embryogenesis.

FIG. 7 shows detection of gene expression by high-density array Southernhybridization for loblolly pine genotype 333 after 12 weeks on twomaturation media.

FIG. 8 depicts the application of embryogenic gene expression studies.

FIG. 9 displays slot blots and expression levels for threeembryogenesis-related genes.

FIG. 10 depicts clone LPS-097 sequence (LP2-3 differential displayfragment.)

FIG. 11 displays a northern blot for the LP2-3 gene during stages 1-3.

FIG. 12 displays a slot blot of total RNA from somatic embryo tissueprobed with an LP2-3-specific probe.

FIG. 13 displays a slot blot of total RNA from zygotic embryo tissueprobed with an LP2-3-specific probe.

FIG. 14 depicts the quantified expression of early zygotic embryoscompared to early somatic embryos.

DETAILED DESCRIPTION OF THE INVENTION

The three hundred and twenty-seven differentially expressed cDNAsisolated from plant specimens of known developmental ages are disclosedin SEQ ID NOS: 1-327. The seven stage-specific promoters isolated fromplant specimens are disclosed in SEQ ID NOS: 328-334. The discovery ofthese cDNAs and promoters enables the design, isolation, andconstruction of related nucleic acids, proteins, antigens, antibodiesother heterologous genes. Both the cDNAs and promoters facilitate thestaging, characterization, and manipulation of plant embyrogenesis, inparticular, conifer embryogenesis. These molecules, and related nucleicacids, peptides, proteins, antigens, and antibodies are particularlyuseful when compiled into a relational database for the monitoring,design, selection, and cultivation of improved crop plants.

The cDNAs of SEQ ID NOS: 1-327, in addition to the promoters of SEQ IDNOS: 328-334, were originally derived from Pinus taeda embryos, commonlyknown as the Loblolly Pine. Nevertheless, it is understood that theinvention is applicable to and encompasses all plants, including alldicotyledonous plants, including all conifers, including all species ofPinus, Picea, and Pseudotsuga. Exemplary conifers may include Piceaabies, and Psedotsuga menziesii, and Pinus taeda.

Nucleic Acid Molecules

In a particular embodiment, the invention relates to certain isolatednucleotide sequences including those that are substantially free fromcontaminating endogenous material. The terms “nucleic acid” or “nucleicacid molecule” refer to a deoxyribonucleotide or ribonucleotide polymerin either single-or double-stranded form, and unless otherwise limited,would encompass known analogs of natural nucleotides that can functionin a similar manner as naturally occurring nucleotides. A “nucleotidesequence” also refers to a polynucleotide molecule or oligonucleotidemolecule in the form of a separate fragment or as a component of alarger nucleic acid. The nucleotide sequence or molecule may also bereferred to as a “nucleotide probe.” The nucleic acid molecules of theinvention are derived from DNA or RNA isolated at least once insubstantially pure form and in a quantity or concentration enablingidentification, manipulation, and recovery of its component nucleotidesequence by standard biochemical methods. Examples of such methods,including methods for PCR protocols that may be used herein, aredisclosed in Sambrook et al., Molecular Cloning: A Laboratory Manual,2nd ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y. (1989),Current Protocols in Molecular Biology edited by F. A. Ausubel et al.,John Wiley and Sons, inc. (1987), and Innis, M. et al., eds., PCRProtocols: A Guide to Methods and Applications, Academic Press (1990),each of which are herein incorporated by reference in their entirety.

As used herein a “nucleotide probe” is defined as an oligonucleotidecapable of binding to a target nucleic acid of complementary sequencethrough one or more types of chemical bonds, through complementary basepairing, or through hydrogen bond formation. As described above, theoligonucleotide probe may include natural (ie. A, G, C, or T) ormodified bases (7-deazaguanosine, inosine, etc.). In addition, bases ina oligonucleotide probe may be joined by a linkage other than aphosphodiester bond, so long as it does not prevent hybridization. Thus,oligonucleotide probes may have constituent bases joined by peptidebonds rather than phosphodiester linkages.

A “target nucleic acid” herein refers to a nucleic acid to which thenucleotide probe or molecule can specifically hybridize. The probe isdesigned to determine the presence or absence of the target nucleicacid, and the amount of target nucleic acid. The target nucleic acid hasa sequence that is complementary to the nucleic acid sequence of thecorresponding probe directed to the target. As recognized by one ofskill in the art, the probe may also contain additional nucleic acids orother moieties, such as labels, which may not specifically hybridize tothe target. The term target nucleic acid may refer to the specificnucleotide sequence of a larger nucleic acid to which the probe isdirected or to the overall sequence (e.g., gene or mRNA) whoseexpression level it is desired to detect. One skilled in the art willrecognize the full utility under various conditions.

As described herein, the nucleic acid molecules of the invention includeDNA in both single-stranded and double-stranded form, as well as the RNAcomplement thereof. DNA includes, for example, cDNA, genomic DNA,chemically synthesized DNA, DNA amplified by PCR, and combinationsthereof. Genomic DNA, including translated, non-translated and controlregions, may be isolated by conventional techniques, e.g., using any oneof the cDNAs of SEQ ID NO: 1 through SEQ ID NO: 327, or suitablefragments thereof, as a probe, to identify a piece of genomic DNA whichcan then be cloned using methods commonly known in the art. In general,nucleic acid molecules within the scope of the invention includesequences that hybridize to sequences of SEQ ID NOS: 1-334 underhybridization and wash conditions of 5°, 10°, 15°, 20°, 25°, or 30°below the melting temperature of the DNA duplex of sequences of SEQ IDNOS: 1-334, including any range of conditions subsumed within theseranges.

DNA Arrays

In a further embodiment, DNA arrays are used to identify hybridizingsequences from test samples. The term “DNA array” refers to “genearrays,” “DNA chips,” “dot array Southerns,” etc. One of skill in theart will appreciate that an enormous number of array designs aresuitable for the practice of this invention. The DNA array willtypically include one or a multiplicity of nucleic acid moleculesderived from SEQ ID NO: 1 through SEQ ID NO: 327 that specificallyhybridize to the nucleic acid expression of which is to be detected. Inaddition, the array may include one or more control probes to monitorthe expression system. Control probes refer to known expression productspresent at each stage of expression, e.g., ribosomal gene products orthe transcripts of other housekeeping genes. The organization of the DNAarray will be known to facilitate interpretation of results. Examples inthe art describing the uses and composition of DNA arrays can be foundin U.S. Pat. Nos.: 5,700,637, 5,837,832, 5,843,655, 5,874,219,6,040,138, 6,045,996, and are incorporated by reference.

Molecules That Hybridize to Identified Sequences

Thus, in a particular embodiment, this invention provides an isolatednucleic acid molecule selected from the group consisting of:

-   (1) a DNA sequence comprising any one of the sequences presented in    SEQ ID NO: 1 through SEQ ID NO: 334;-   (2) an isolated nucleic acid molecule that hybridizes to either    strand of a denatured, double-stranded DNA comprising the nucleic    acid sequence of (a) under conditions of moderate stringency; and-   (3) an isolated nucleic acid molecule that hybridizes to either    strand of a denatured, double-stranded DNA comprising the nucleic    acid sequence of (a) under conditions of high stringency.

As used herein, stringency conditions in nucleic acid hybridizations canbe readily determined by those having ordinary skill in the art basedon, for example, the length and composition of the nucleic acid. In oneembodiment, moderate stringency is herein defined as a nucleic acidhaving 10, 11, 12, 13, 14, 15, 16, or 17, contiguous nucleotidesidentical to any of the sequences of SEQ ID NOS: 1-334, or a complementthereof. Similarly, high stringency is hereby defined as a nucleic acidhaving 18, 19, 20, 21, 22, or more contiguous identical nucleotides, ora longer nucleic acid having at least 80, 85, 90, 95, or 99 percentidentity with any of the sequences of SEQ ID NOS: 1-334; for sequencesof at least 50, 100, 150, 200, or 250 nucleotides, high stringency maycomprise an overall identity of at least 60, 65, 70 or 75 percent.

Generally, nucleic acid hybridization simply involves providing adenatured nucleotide molecule or probe and target nucleic acid underconditions where the probe and its complementary target can form stablehybrid duplexes through complementary base pairing. The nucleic acidsthat do not substantially form hybrid duplexes are then washed awayleaving the hybridized nucleic acids to be detected, typically throughdetection of an attached detectable label. It is further generallyrecognized that nucleic acids are denatured by increasing thetemperature or decreasing the salt concentration of the buffercontaining the nucleic acids. Under lower stringency conditions (e.g.,low temperature and/or high salt) hybrid duplexes (e.g., DNA:DNA,RNA:RNA, or RNA:DNA) will form even where the annealed sequences are notperfectly complementary. Thus specificity of hybridization is reduced atlower stringency. Conversely, at higher stringency (e.g., highertemperature or lower salt) successful hybridization requires fewermismatches. One of skill in the art will appreciate that hybridizationconditions may be selected to provide any degree of stringency.

As used herein, the percent identity between an amino acid sequenceencoded by any of SEQ ID NOS: 1-334 and a potential hybridizing variantcan be determined, for example, by comparing sequence information usingthe GAP computer program, version 6.0 described by Devereux et al.(Nucl. Acids Res. 12:387, 1984) and available from the University ofWisconsin Genetics Computer Group (UWGCG). The GAP program utilizes thealignment method of Needleman and Wunsch (J. Mol. Biol, 48:443, 1970),as revised by Smith and Waterman (Adv. Appl. Math 2:482, 1981). Thepreferred default parameters for the GAP program include: (1) a unarycomparison matrix (containing a value of 1 for identities and 0 fornon-identities) for nucleotides, and the weighted comparison matrix ofGribskov and Burgess (Nuci. Acids Res. 14:6745, 1986), as described bySchwartz and Dayhoff (eds., Atlas of Protein Sequence and Structure,National Biomedical Research Foundation, pp. 353-358, 1979); (2) apenalty of 3.0 for each gap and an additional 0.10 penalty for eachsymbol in each gap; and (3) no penalty for end gaps.

Alternatively, basic protocols for empirically determining hybridizationstringency are set forth in section 2.10 of Current Protocols inMolecular Biology edited by F. A. Ausubel et al., John Wiley and Sons,Inc. (1987). Stringency conditions can be determined readily by theskilled artisan. An example of moderate stringency hybridizationconditions would be hybridization in 5×SSC, 5× Denhardt's Solution, 50%(w/v) formamide, and 1% SDS at 42° C. with washing conditions of 0.2×SSCand 0.1% SDS at 42° C. An example of high stringency conditions can bedefined as hybridization conditions as above, and with washing atapproximately 68° C., in 0.1×SSC, and 0.1% SDS. The skilled artisan willrecognize that the temperature and wash solution salt concentration canbe adjusted as necessary according to factors such as the length of theprobe.

Due to the degeneracy of the genetic code wherein more than one codoncan encode the same amino acid, multiple DNA sequences can code for thesame polypeptide. Such variant DNA sequences can result from geneticdrift or artificial manipulation (e.g., occurring during PCRamplification or as the product of deliberate mutagenesis of a nativesequence). The present invention thus encompasses any nucleic acidcapable of encoding a protein derived from SEQ ID NOS: 1-327, orvariants thereof.

Deliberate mutagenesis of a native sequence can be carried out usingnumerous techniques well known in the art. For example,oligonucleotide-directed site-specific mutagenesis procedures can beemployed, particularly where it is desired to mutate a gene such thatpredetermined restriction nucleotides or codons are altered bysubstitution, deletion or insertion. Exemplary methods of making suchalterations are disclosed by Walder et al. (Gene 42:133, 1986); Bauer etal. (Gene 37:73, 1985); Craik (BioTechniques, Jan. 12-19, 1985); Smithet al. (Genetic Engineering: Principles and Methods, Plenum Press,1981); Kunkel (Proc. Natl. Acad. Sci. USA 82:488, 1985); Kunkel et al.(Methods in Enzymol. 154:367, 1987); and U.S. Pat. Nos. 4,518,584 and4,737,462, all of which are incorporated by reference.

Thus, the invention further provides an isolated nucleic acid moleculeselected from the group comprising of (1), (2), and (3) above andfurther consisting of:

-   (4) an isolated nucleic acid molecule degenerate from SEQ ID NOS:    1-334 as a result of the genetic code; and-   (5) an isolated nucleic acid molecule selected from the group    consisting of an allelic variants and species homologs of SEQ ID    NOS: 1-334.    Obtaining Full Length cDNAs

The cDNAs isolated and cloned through the differential display procedurewill often only represent a partial sequence (generally the 3′ end) ofthe mRNA from which it was derived due to the nature of the arbitraryprimer used in the differential display PCR reaction. Consequently, thecDNA sequences of SEQ ID NOS: 1-327 provide powerful tools for obtainingthe sequences of full-length cDNAs. This can be accomplished by using apartial cDNA as a probe to identify and isolate the full length cDNAfrom a population of full length cDNAs or from a full length cDNAlibrary. As is well known in the art, similar procedures can be used toidentify corresponding genomic DNA sequences.

Alternatively, one can obtain the 5′ sequence of a partial cDNA byperforming Rapid Amplification of cDNA Ends (RACE) procedures such asthose disclosed in Frohman, Methods in Enzymology, 218:340-356 (1993)and Bertling et al., PCR Methods and Applications 3:95-99 (1993) whichare hereby incorporated by reference. For example, ClonetechLaboratories, Inc. (Palo Alto, Calif.) offers a SMAR™ cDNA product linethat allows one to generate high quality full length cDNAs and cDNAlibraries. SMART™ technology can also be used to perform RACE. Oneskilled in the art will readily recognize that there are otherequivalent products and procedures for obtaining full length cDNAs. Fulllength cDNAs may be sequenced and their sequences compared to sequencesin public databases to assess their identities and/or homologies toother known sequences.

Cloned full length cDNAs can be used in the construction of expressionvectors for the production and purification of pine tree polypeptideswhich contain the pine tree peptides encoded by the cDNAs of any one ofSEQ ID NOS: 1-327.

Oligonucleotide Primers for PCR Assays

In another embodiment, the present invention encompasses oligonucleotidefragments derived from any one of SEQ ID NO: 1 through SEQ ID NO: 327 orfrom the reverse complement sequence of any one of SEQ ID NO: 1 throughSEQ ID NO: 327. Such oligonucleotides would be useful as primers in theperformance of RT-PCR assays to detect, or even quantify, pine embryostage-specific transcripts. Such oligonucleotide primers will generallycomprise from 10 to 25 nucleotides substantially complementary to theends of the target sequence and may contain additional non-complementarynucleotides, for example, nucleotides that generate a restrictionendonuclease site or cloning junction. Programs useful in selecting PCRprimers may be used to design the oligonucleotides of this invention,but use of such programs is not necessary. By way of example, theWisconsin Package™ software available from the Genetic Computer Group(Madison, Wis.) includes a program called Prime that can aid inselecting primers from a given template sequence. Protocols for thedesign and optimization of PCR reactions are commonly known in the artand are described in Saiki et al., Science 239:487 (1988); RecombinantDNA Methodology, Wu et al., eds., Academic Press, Inc., San Diego(1989), pp. 189-196; and PCR Protocols: A Guide to Methods andApplications, Innis et al., eds., Academic Press, Inc. (1990).

Antisense Nucleic Acid Molecules

Other useful fragments of the nucleic acids include antisense or senseoligonucleotides comprising a single-stranded nucleic acid sequence(either RNA or DNA) capable of binding to target mRNA (sense) or DNA(antisense) sequences. Antisense or sense oligonucleotides, according tothe present invention, comprise a fragment of DNA from any one of SEQ IDNO: 1 through SEQ ID NO: 327. Such a fragment generally comprises atleast about 14 nucleotides, preferably from about 14 To about 30nucleotides. The ability to derive an antisense or a senseoligonucleotide, based upon a cDNA sequence encoding a given protein isdescribed in, for example, Stein and Cohen (Cancer Res. 48:2659, 1988)and van der Krol et al. (Bio/Techniques 6:958, 1988).

Binding of antisense or sense oligonucleotides to target nucleic acidsequences results in the formation of duplexes or other nucleic acidcomplexes inimical to efficient production of gene products. Theantisense oligonucleotides thus may be used to block expression ofproteins or the function of RNA. Antisense or sense oligonucleotidesfurther comprise oligonucleotides having modified sugar-phosphodiesterbackbones (or other sugar linkages, such as those described inWO91/06629) and wherein such sugar linkages are resistant to endogenousnucleases. Such oligonucleotides with resistant sugar linkages arestable in vivo (i.e., capable of resisting enzymatic degradation) butretain sufficient sequence specificity to be able to bind to targetnucleotide sequences.

Other examples of sense or antisense oligonucleotides include thoseoligonucleotides which are covalently linked to organic moieties, suchas those described in WO 90/10448, and other moieties that increasesaffinity of the oligonucleotide for a target nucleic acid sequence, suchas poly-(L-lysine). Further still, intercalating agents, such asellipticine, and alkylating agents or metal complexes may be attached tosense or antisense oligonucleotides. Such modifications may modifybinding specificities of the antisense or sense oligonucleotide for thetarget nucleotide sequence.

Antisense or sense oligonucleotides may be introduced into a cellcontaining the target nucleic acid sequence by any gene transfer method,including, for example, lipofection, CaPO⁴-mediated DNA transfection,electroporation, or by using gene transfer vectors such as Epstein-Barrvirus or adenovirus.

Sense or antisense oligonucleotides also may be introduced into a cellcontaining the target nucleotide sequence by formation of a conjugatewith a ligand binding molecule, as described in WO 91/04753. Suitableligand binding molecules include, but are not limited to, cell surfacereceptors, growth factors, other cytokines, or other ligands that bindto cell surface receptors. In one embodiment, conjugation of the ligandbinding molecule does not substantially interfere with the ability ofthe ligand binding molecule to bind to its corresponding molecule orreceptor, or block entry of the sense or antisense oligonucleotide orits conjugated version into the cell.

Alternatively, a sense or an antisense oligonucleotide may be introducedinto a cell containing the target nucleic acid sequence by formation ofan oligonucleotide-lipid complex, as described in WO 90/10448. The senseor antisense oligonucleotide-lipid complex is preferably dissociatedwithin the cell by an endogenous lipase.

Polypeptides Encoded by Differentially-Expressed cDNAs

The cDNAs of SEQ ID NOS: 1-327 can be translated into amino acidsequences potentially corresponding to portions ofdevelopmentally-regulated plant proteins. These amino acid sequences canbe identified from sequences listed in Table I, below. The cDNAsencoding these predicted polypeptides are grouped into early, middle,and late transcripts according to the staged embryo population fromwhich they were derived.

(See Table I)

Although the term “peptide” is generally understood to referencesynthetic sequences, or fragments of larger proteins, and includes shortamino acid sequences of between 2 and 10 amino acids, whereas“polypeptide” refers to larger sequences and full-length proteins, theterms are used interchangeably herein to indicate that the inventionapplies to peptides and polypeptides of any length and variants thereof.Moreover, the discovery of presumptive open reading frames in SEQ IDNOS: 1-327, and the ability to isolate additional cDNA sequence, enablesthe construction of expression vectors comprising nucleic acid sequencesencoding those polypeptides. The cDNAs of the invention also enablecells transfected or transformed with expression vectors driving theexpression of the encoded polypeptides and antibodies reactive with thepolypeptides.

In one embodiment, the invention provides for isolated polypeptides,preferably, pine tree polypeptides. As used herein, the term“polypeptides” refers to a genus of polypeptide or peptide fragmentsthat encompass the amino acid sequences identified from Table I, as wellas smaller fragments. Consequently, the invention encompasses anypolypeptide fragment comprising at least 5, 6, 7, 8, 9, 10, 11, 12, 13,14, or 15 contiguous amino acids encoded by the cDNAs of any of SEQ IDNOS: 1-327, or comprising at least 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or15 contiguous amino acids of any of amino acid sequence derived fromTable I.

Alternatively, a polypeptide may be defined in terms of its antigenicrelatedness to any peptide encoded by SEQ ID NOS:-1-327. Thus, in oneembodiment a polypeptide within the scope of the invention is defined asan amino acid sequence comprising a linear or 3-dimensional epitopeshared with any peptide encoded by the cDNAs of SEQ ID NOS: 1-327.Alternatively, a polypeptide within the scope of the invention isrecognized by an antibody that specifically recognizes any peptideencoded by SEQ ID NOS: 1-327. Antibodies are defined to be specificallybinding if they bind pine tree polypeptides with a K_(a) of greater thanor equal to about 10⁷ M⁻, and preferably greater than or equal to 10⁸M⁻¹.

A polypeptide “variant” as referred to herein means a polypeptidesubstantially homologous to a native polypeptide, but which has an aminoacid sequence different from that encoded by any of SEQ ID NOS: 1-327because of one or more deletions, insertions or substitutions. Thevariant amino acid sequence preferably is at least 80% identical to anative polypeptide amino acid sequence, preferably at least 90%, morepreferably, at least 95% identical over at least 6, 7, 8, 9, 10, 11, 12,13, 14, 15, 16, 17, 18, 19, 20, 21-25, or 26-30 contiguous amino acids.The percent identity between an amino acid sequence encoded by any ofSEQ ID NOS: 1-327 and a potential variant can be determined manually,or, for example, by comparing sequence information using the GAPcomputer program, version 6.0 described by Devereux et al. (Nucl. AcidsRes. 12:387, 1984) and available from the University of WisconsinGenetics Computer Group (UWGCG). The GAP program, described above,utilizes the alignment method of Needleman and Wunsch (J. Mol. Biol.48:443,1970), as revised by Smith and Waterman (Adv. Appl. Math 2:482,1981).

Variants can comprise conservatively substituted sequences, meaning thata given amino acid residue is replaced by a residue having similarphysiochemical characteristics. Examples of conservative substitutionsinclude substitution of one aliphatic residue for another, such as lie,Val, Leu, or Ala for one another, or substitutions of one polar residuefor another, such as between Lys and Arg; Glu and Asp; or Gin and Asn.See Zubay, Biochemistry, Addison-Wesley Pub. Co., (1983) incorporated byreference in its entirety. The effects of such substitutions can becalculated using substitution score matrices such a PAM-120, PAM-200,and PAM-250 as discussed in Altschul, (J. Mol. Biol. 219:555-65, 1991).Other such conservative substitutions, for example, substitutions ofentire regions having similar hydrophobicity characteristics, are wellknown.

Naturally-occurring peptide variants are also encompassed by theinvention. Examples of such variants are proteins that result fromalternate mRNA splicing events or from proteolytic cleavage of thepolypeptides of Table I. Variations attributable to proteolysis include,for example, differences in the N- or C-termini upon expression indifferent types of host cells, due to proteolytic removal of one or moreterminal amino acids from the polypeptides encoded by the sequences ofTable I (generally from 1-5 terminal amino acids).

As stated above, the invention provides recombinant and non-recombinant,isolated and purified polypeptides, preferably pine tree polypeptides.Variants and derivatives of native polypeptides can be obtained byisolating naturally-occurring variants, or the nucleotide sequence ofvariants, of other plant lines or species, or by artificiallyprogramming mutations of nucleotide sequences coding for native pinetree polypeptides. Alterations of the native amino acid sequence can beaccomplished by any of a number of conventional methods. Mutations canbe introduced at particular loci by synthesizing oligonucleotidescontaining a mutant sequence, flanked by restriction sites enablingligation to fragments of the native sequence. Following ligation, theresulting reconstructed sequence encodes an analog having the desiredamino acid insertion, substitution, or deletion. Alternatively,oligonucleotide-directed site-specific mutagenesis procedures can beemployed to provide an altered gene wherein predetermined codons can bealtered by substitution, deletion or insertion. Exemplary methods ofmaking such alterations are discussed supra.

The following sections are examples of the various expression vectors,host cells, and protein purification methods that are known in the art.These examples are provided merely as illustrative and should not beconstrued as the only means to express and purify the polypeptides andpolypeptide variants of the invention.

Expression Vectors and Purified proteins

Recombinant expression vectors containing a nucleic acid sequenceencoding the polypeptides of the invention can be prepared using wellknown methods. In one embodiment, the expression vectors include a cDNAsequence encoding the polypeptide operably linked to suitabletranscriptional or translational regulatory nucleotide sequences, suchas those derived from a mammalian, microbial, viral, or insect gene.Examples of regulatory sequences include transcriptional promoters,operators, or enhancers, mRNA ribosomal binding sites, and appropriatesequences which control transcription and translation initiation andtermination. Nucleotide sequences are “operably linked” when theregulatory sequence functionally relates to the cDNA sequence of theinvention. Thus, a promoter nucleotide sequence is operably linked to acDNA sequence if the promoter nucleotide sequence controls thetranscription of the cDNA sequence. The ability to replicate in thedesired host cells, usually conferred by an origin of replication, and aselection gene by which transformants are identified can additionally beincorporated into the expression vector.

In addition, sequences encoding appropriate signal peptides that are notnaturally associated with the polypeptides of the invention can beincorporated into expression vectors. For example, a DNA sequence for asignal peptide (secretory leader) can be fused in-frame to the pine treenucleotide sequence so that the polypeptides of the invention isinitially translated as a fusion protein comprising the signal peptide.A signal peptide that is functional in the intended host cells enhancesextracellular secretion of the expressed polypeptide. The signal peptidecan be cleaved from the polypeptide upon secretion from the cell.

Fusions of additional peptide sequences at the amino and carboxylterminal ends of the polypeptides of the invention can be used toenhance expression of the polypeptides or aid in the purification of theprotein. Such peptides include, for example, poly-His or the antigenicidentification peptides described in U.S. Pat. No. 5,011,912 and in Hoppet al., (Bio/Technology6:1204, 1988).

Suitable host cells for expression of polypeptides of the inventioninclude prokaryotes, yeast or higher eukaryotic cells. Appropriatecloning and expression vectors for use with bacterial, fungal, yeast,and mammalian cellular hosts are described, for example, in Pouwels etal., Cloning Vectors: A Laboratory Manual, Elsevier, N.Y., (1985).Cell-free translation systems could also be employed to the disclosedpolypeptides using RNAs derived from DNA constructs disclosed herein.

Prokaryotic Expression Systems

Prokaryotes include gram negative or gram positive organisms, forexample, E. coli or Bacilli. Suitable prokaryotic host cells fortransformation include, for example, E. coli, Bacillus subtilis,Salmonella typhimurium, and various other species within the generaPseudomonas, Streptomyces, and Staphylococcus. In a prokaryotic hostcell, such as E. coli, the disclosed polypeptides can include anN-terminal methionine residue to facilitate expression of therecombinant polypeptide in the prokaryotic host cell. The N-terminalmethionine can be cleaved from the expressed recombinant polypeptide.

Expression vectors for use in prokaryotic host cells generally compriseone or more phenotypic selectable marker genes. A phenotypic selectablemarker gene is, for example, a gene encoding a protein that confersantibiotic resistance or that supplies an autotrophic requirement.Examples of useful expression vectors for prokaryotic host cells includethose derived from commercially available plasmids such as the cloningvector pBR322 (ATCC 37017). pBR322 contains genes for ampicillin andtetracycline resistance and thus provides simple means for identifyingtransformed cells. To construct an expression vector using pBR322, anappropriate promoter and a DNA sequence encoding one or more of thepolypeptides of the invention are inserted into the pBR322 vector. Othercommercially available vectors include, for example, pKK223-3 (PharmaciaFine Chemicals, Uppsala, Sweden) and pGEM-1 (Promega Biotec, Madison,Wis., USA). Other commercially available vectors include those that arespecifically designed for the expression of proteins; these wouldinclude pMAL-p2 and pMAL-c2 vectors that are used for the expression ofproteins fused to maltose binding protein (New England Biolabs, Beverly,Mass., USA).

Promoter sequences commonly used for recombinant prokaryotic host cellexpression vectors include P-lactamase (penicillinase), lactose promotersystem (Chang et al., Nature 275:615, 1978; and Goeddel et al., Nature281:544, 1979), tryptophan (trp) promoter system (Goeddel et al., Nucl.Acids Res. 8:4057, 1980; and EP-A-36776), and tac promoter (Maniatis,Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory,p. 412, 1982). A particularly useful prokaryotic host cell expressionsystem employs a phage λ P_(L) promoter and a c1857ts thermolabilerepressor sequence. Plasmid vectors available from the American TypeCulture Collection (“ATCC”), which incorporate derivatives of the PLpromoter, include plasmid pHUB2 (resident in E. Coli strain JMB9 (ATCC37092)) and pPLc28 (resident in E. coli RR1 (ATCC 53082)).

DNA encoding one or more of the polypeptides of the invention may becloned in-frame into the multiple cloning site of an ordinary bacterialexpression vector. Ideally the vector would contain an induciblepromoter upstream of the cloning site, such that addition of an inducerleads to high-level production of the recombinant protein at a time ofthe investigator's choosing. For some proteins, expression levels may beboosted by incorporation of codons encoding a fusion partner (such ashexahistidine) between the promoter and the gene of interest. Theresulting “expression plasmid” may be propagated in a variety of strainsof E. coli.

For expression of the recombinant protein, the bacterial cells arepropagated in growth medium until reaching a pre-determined opticaldensity. Expression of the recombinant protein is then induced, e.g., byaddition of IPTG (isopropyl-b-D-thiogalactopyranoside), which activatesexpression of proteins from plasmids containing a lac operator/promoter.After induction (typically for 1-4 hours), the cells are harvested bypelleting in a centrifuge, e.g., at 5,000×G for 20 minutes at 4° C.

For recovery of the expressed protein, the pelleted cells may beresuspended in ten volumes of 50 mM Tris-HCl (pH 8)/1 M NaCl and thenpassed two or three times through a French press. Most highly expressedrecombinant proteins forms insoluble aggregates known as inclusionbodies. Inclusion bodies can be purified away from the soluble proteinsby pelleting in a centrifuge at 5,000×G for 20 minutes, 4° C. Theinclusion body pellet is washed with 50 mM Tris-HCl (pH 8)/1% TritonX-100 and then dissolved in 50 mM Tris-HCl (pH 8)/8 M urea/0.1 M DTT.Any material that cannot be dissolved in 50 mM Tris-HCl (pH 8)/8 Murea/0.1 M DTT may be removed by centrifugation (10,000×G for 20minutes, 20° C.). The protein of interest will, in most cases, be themost abundant protein in the resulting clarified supernatant. Thisprotein may be “refolded” into the active conformation by dialysisagainst 50 mM Tris-HCl (pH 8)/5 mM CaCl₂/5 mM Zn(OAc)₂/1 mM GSSG/0.1 mMGSH. After refolding, purification can be carried out by a variety ofchromatographic methods such as ion exchange or gel filtration. In someprotocols, initial purification may be carried out before refolding. Asan example, hexahistidine-tagged fusion proteins may be partiallypurified on immobilized Nickel.

While the preceding purification and refolding procedure assumes thatthe protein is best recovered from inclusion bodies, those skilled inthe art of protein purification will appreciate that many recombinantproteins are best purified out of the soluble fraction of cell lysates.In these cases, refolding is often not required, and purification bystandard chromatographic methods can be carried out directly.

Yeast Expression Systems

Polypeptides of the invention can also be expressed in yeast host cells,preferably from the Saccharomyces genus (e.g., S. cerevisiae). Othergenera of yeast, such as Pichia or Kluyveromyces (e.g. K. lactis), canalso be employed. Yeast vectors will often contain an origin ofreplication sequence from a 2μ yeast plasmid, an autonomouslyreplicating sequence (ARS), a promoter region, sequences forpolyadenylation, sequences for transcription termination, and aselectable marker gene. Suitable promoter sequences for yeast vectorsinclude, among others, promoters for metallothionine, 3-phosphoglyceratekinase (Hitzeman et al., J. Biol. Chem. 255:2073, 1980), or otherglycolytic enzymes (Hess et al., J. Adv. Enzyme Reg. 7:149, 1968; andHolland et al., Biochem. 17:4900, 1978), such as enolase,glyceraldehyde-3-phosphate dehydrogenase, hexokinase, pyruvatedecarboxylase, phosphofructokinase, glucose-6-phosphate isomerase,3-phosphoglycerate mutase, pyruvate kinase, triosephosphate isomerase,phosphoglucose isomerase, and glucokinase. Other suitable vectors andpromoters for use in yeast expression are further described in Hitzeman,EPA-73,657 or in Fleer et. al., Gene, 107:285-195 (1991); and van denBerg et. al., Bio/Technology, 8:135-139 (1990). Another alternative isthe glucose-repressible ADH2 promoter described by Russell et al. (J.Biol. Chem. 258:2674, 1982) and Beier et al. (Nature 300:724, 1982).Shuttle vectors replicable in both yeast and E. coli can be constructedby inserting DNA sequences from pBR322 for selection and replication inE. coli (Amp gene and origin of replication) into the above-describedyeast vectors.

The yeast α-factor leader sequence can be employed to direct secretionof one or more of the disclosed polypeptides. The α-factor leadersequence is often inserted between the promoter sequence and thestructural gene sequence. See, e.g., Kurjan et al., Cell 30:933, 1982;Bitter et al., Proc. Natl. Acad. Sci. USA 81:5330, 1984; U.S. Pat. No.4,546,082; and EP 324,274. Other leader sequences suitable forfacilitating secretion of recombinant polypeptides from yeast hosts areknown to those of skill in the art. A leader sequence can be modifiednear its 3′ end to contain one or more restriction sites. This willfacilitate fusion of the leader sequence to the structural gene.

Yeast transformation protocols are known to those of skill in the art.One such protocol is described by Hinnen et al., Proc. Natl. Acad. Sci.USA 75:1929, 1978. The Hinnen et al. protocol selects forTrp+transformants in a selective medium, wherein the selective mediumconsists of 0.67% yeast nitrogen base, 0.5% casamino acids, 2% glucose,10 μg/ml adenine, and 20 μg/ml uracil.

Yeast host cells transformed by vectors containing ADH2 promotersequence can be grown for inducing expression in a “rich” medium. Anexample of a rich medium is one consisting of 1% yeast extract, 2%peptone, and 1% glucose supplemented with 80 μg/ml adenine and 80 μg/mluracil. Derepression of the ADH2 promoter occurs when glucose isexhausted from the medium.

Mammalian Expression Systems

Mammalian or insect host cell culture systems could also be employed toexpress recombinant polypeptides of the invention. Baculovirus systemsfor production of heterologous proteins in insect cells are reviewed byLuckow and Summers, Bio/Technology 6:47 (1988). Established cell linesof mammalian origin also can be employed. Examples of suitable mammalianhost cell lines include the COS-7 line of monkey kidney cells (ATCC CRL1651) (Gluzman et al., Cell 23:175, 1981), L cells, C127 cells, 3T3cells (ATCC CCL 163), Chinese hamster ovary (CHO) cells, HeLa cells, andBHK (ATCC CRL 10) cell lines, and the CV-1/EBNA-1 cell line (ATCC CRL10478) derived from the African green monkey kidney cell line CVI (ATCCCCL 70) as described by McMahan et al. (EMBO J. 10: 2821, 1991).

Established methods for introducing DNA into mammalian cells have beendescribed (Kaufman, R. J., Large Scale Mammalian Cell Culture, 1990, pp.15-69). Additional protocols using commercially available reagents, suchas Lipofectamine (Gibco/BRL) or Lipofectamine-Plus, can be used totransfect cells (Feigner et al., Proc. Natl. Acad. Sci. USA84:7413-7417, 1987). In addition, electroporation can be used totransfect mammalian cells using conventional procedures, such as thosein Sambrook et al. Molecular Cloning: A Laboratory Manual, 2 ed. Vol.1-3, Cold Spring Harbor Laboratory Press, 1989). Selection of stabletransformants can be performed using resistance to cytotoxic drugs as aselection method. Kaufman et al., Meth. in Enzymology 185:487-511, 1990,describes several selection schemes, such as dihydrofolate reductase(DHFR) resistance. A suitable host strain for DHFR selection can be CHOstrain DX-B11, which is deficient in DHFR (Urlaub and Chasin, Proc.Natl. Acad. Sci. USA 77:4216-4220, 1980). A plasmid expressing the DHFRcDNA can be introduced into strain DX-B11, and only cells that containthe plasmid can grow in the appropriate selective media. Other examplesof selectable markers that can be incorporated into an expression vectorinclude cDNAs conferring resistance to antibiotcs, such as G418 andhygromycin B. Cells harboring the vector can be selected on the basis ofresistance to these compounds.

Transcriptional and translational control sequences for mammalian hostcell expression vectors can be excised from viral genomes. Commonly usedpromoter sequences and enhancer sequences are derived from polyomavirus, adenovirus 2, simian virus 40 (SV40), and human cytomegalovirus.DNA sequences derived from the SV40 viral genome, for example, SV40origin, early and later promoter, enhancer, splice, and polyadenylationsites can be used to provide other genetic elements for expression of astructural gene sequence in a mammalian host cell. Viral early and latepromoters are particularly useful because both are easily obtained froma viral genome as a fragment, which can also contain a viral origin ofreplication (Fiers et al., Nature 273:113, 1978; Kaufman, Meth. inEnzymology, 1990). Smaller or larger SV40 fragments can also be used,provided the approximately 250 bp sequence extending from the Hind IIIsite toward the Bgl I site located in the SV40 viral origin ofreplication site is included.

Additional control sequences shown to improve expression of heterologousgenes from mammalian expression vectors include such elements as theexpression augmenting sequence element (EASE) derived from CHO cells(Morris et al., Animal Cell Technology, 1997, pp. 529-534) and thetripartite leader (TPL) and VA gene RNAs from Adenovirus 2 (Gingeras etal., J. Biol. Chem. 257:13475-13491,1982). The internal ribosome entrysite (IRES) sequences of viral origin allows dicistronic mRNAs to betranslated efficiently (Oh and Sarnow, Current Opinion in Genetics andDevelopment 3:295-300, 1993; Ramesh et al., Nucleic Acids Research24:2697-2700, 1996). Expression of a heterologous cDNA as part of adicistronic mRNA followed by the gene for a selectable marker (eg. DHFR)has been shown to improve transfectability of the host and expression ofthe heterologous cDNA (Kaufman, Meth. in Enzymology, 1990). Exemplaryexpression vectors that employ dicistronic mRNAs are pTR-DC/GFPdescribed by Mosser et al., Biotechniques 22:150-161, 1997, and p2A51described by Morris et al., Animal Cell Technology, 1997, pp. 529-534.

A useful high expression vector, pCAVNOT, has been described by Mosleyet al., Cell 59:335-348,1989. Other expression vectors for use inmammalian host cells can be constructed as disclosed by Okayama and Berg(Mol. Cell. Biol. 3:280, 1983). A useful system for stable high levelexpression of mammalian cDNAs in C127 murine mammary epithelial cellscan be constructed substantially as described by Cosman et al. (Mol.Immunol. 23:935, 1986). A useful high expression vector, PMLSV N1/N4,described by Cosman et al., Nature 312:768, 1984, has been deposited asATCC 39890. Additional useful mammalian expression vectors are describedin EP-A-0367566, and in U.S. patent application Ser. No. 07/701,415,filed May 16, 1991, incorporated by reference herein. The vectors can bederived from retroviruses. In place of the native signal sequence, aheterologous signal sequence can be added, such as the signal sequencefor IL-7 described in U.S. Pat. No. 4,965,195; the signal sequence forIL-2 receptor described in Cosman et al., Nature 312:768 (1984); the IL4signal peptide described in EP 367,566; the type I IL-1 receptor signalpeptide described in U.S. Pat. No. 4,968,607; and the type H IL-1receptor signal peptide described in EP 460,846.

The polypeptides of the invention and the nucleic acid moleculesencoding them can also be used as reagents to identify (a) proteins thatthe disclosed polypeptides or their constituent proteins regulate, and(b) other proteins with which it might interact. The disclosedpolypeptides can be coupled to a recombinant protein, to an affinitymatrix, or by using them as a bait in the yeast two-hybrid system. Theuse of the yeast two-hybrid system developed by Stanley Fields andcoworkers is well known in the art and described in Golemis, E., et alSection 20.1 in: Current Protocols in Molecular Biology, ed. Ausubel, F.M., et al., John Wiley & Sons, NY, 1997 and in The Yeast Two-HybridSystem., ed. P. L. Bartel and S. Fields, Oxford University Press, 1997.

Antibodies and Peptide Binding Proteins

Purified polypeptides of the invention can be used to generateantibodies that bind to one or more epitopes of the disclosedpolypeptide. Such anti-polypeptide antibodies includes polyclonalantibodies, monoclonal antibodies, fragments thereof such as F(ab′)₂,and Fab fragments, as well as any recombinantly produced bindingpartners. Antibodies are defined to be specifically binding if they bindpine tree polypeptides with a K_(a) of greater than or equal to about10⁷ M⁻¹. Affinities of binding partners or antibodies can be readilydetermined using conventional techniques, for example, those describedby Scatchard et al., Ann. N.Y. Acad. Sci., 51:660 (1949).

Polyclonal antibodies can be readily generated from a variety ofsources, for example, horses, cows, goats, sheep, dogs, chickens,rabbits, mice, hamsters, guinea pigs, or rats, using procedures that arewell-known in the art, for example, as described for example, U.S. Pat.No. 5,585,100, incorporated by reference herein. In general, acomposition comprising at least one of the polypeptides of the inventionis administered to the host animal, typically through intra-peritonealor subcutaneous injection. In the case where a peptide is used as theimmunogen, it is preferable to conjugated it to a suitable carriermolecule, such as a T-dependent antigen (Bovine Serum Albumin, choleratoxin, and the like). The immunogenicity of the disclosed polypeptidescan also be enhanced through the use of an adjuvant, for example,Freund's complete or incomplete adjuvant or alum. Following boosterimmunizations, small samples of serum are collected and tested forreactivity to the disclosed polypeptides or their constituent epitopes.Examples of various assays useful for such determination include thosedescribed in: Antibodies: A Laboratory Manual, Harlow and Lane (eds.),Cold Spring Harbor Laboratory Press, 1988; as well as procedures such ascountercurrent immuno-electrophoresis (CIEP), radioimmunoassay,radio-immunoprecipitation, enzyme-linked immuno-sorbent assays (ELISA),dot blot assays, and sandwich assays, see U.S. Pat. Nos. 4,376,110 and4,486,530, each of which is incorporated by reference in their entirety.

Monoclonal antibodies (or fragments thereof), directed against epitopesof the disclosed polypeptides can also be readily prepared usingwell-known procedures, such as, for example, the procedures described inU.S. Patent No. RE 32,011, U.S. Pat. Nos. 4,902,614, 4,543,439, and4,411,993; Monoclonal Antibodies, Hybrddomas: A New Dimension inBiological Analyses, Plenum Press, Kennett, McKearn, and Bechtol (eds.),1980, each of which is incorporated by reference. Briefly, the hostanimals, such as mice, are injected intraperitoneally at least once, andpreferably at least twice at about 3 week intervals with isolated andpurified polypeptides optionally in the presence of adjuvant. Again, ifpeptide fragments are used they may need to be conjugated to a suitablecarrier protein. Mouse sera are then assayed by conventional dot blottechnique or antibody capture (ABC) to determine which animal is best tofuse. Approximately two to three weeks later, the mice are given anintravenous boost of pine tree polypeptides. Mice are later sacrificedand spleen cells fused with commercially available myeloma cells, suchas Ag8.653 (ATCC), following established protocols. Briefly, the myelomacells are washed several times in media and fused to mouse spleen cellsat a ratio of about three spleen cells to one myeloma cell. The fusingagent can be any suitable agent used in the art, for example,polyethylene glycol (PEG). Fusion is plated out into plates containingmedia that allows for the selective growth of the fused cells. The fusedcells can then be allowed to grow for approximately eight days.Supernatants from resultant hybridomas are collected and added to aplate that is first coated with goat anti-mouse Ig. Following washes, alabel, such as, ¹²⁵I-pine tree polypeptides is added to each wellfollowed by incubation. Positive wells can be subsequently detected byautoradiography. Positive clones can be grown in bulk culture andsupernatants are subsequently purified over a Protein A column(Pharmacia).

Monoclonal antibodies and specific-binding fragments of the inventioncan be produced using alternative techniques, such as those described byAlting-Mees et al., “Monoclonal Antibody Expression Libraries: A RapidAlternative to Hybridomas”, Strategies in Molecular Biology 3:1-9(1990), which is incorporated herein by reference. Similarly, bindingpartners can be constructed using recombinant DNA techniques toincorporate the variable regions of a gene that encodes a specificbinding antibody Such a technique is described in Larrick et al.,Biotechnology, 7:394 (1989).

It is understood of course that many techniques could be used togenerate antibodies against the polypeptides of the invention and thatthe above embodiments in no way limits the scope of the invention.

Nucleotides. Proteins, Antibodies, and Binding Proteins As Probes andReagents

The disclosed nucleic acids, polypeptides, and antibodies directedagainst the disclosed polypeptides can be used in a variety of researchprotocols, such as in DNA arrays or as reagents. A sample of suchresearch protocols are given in Sambrook et al. Molecular Cloning: ALaboratory Manual, 2 ed. Vol. 1-3, Cold Spring Harbor Laboratory Press,(1989), incorporated by reference. For example, the compiled sequences,polypeptides, etc., can serve as markers for cell specific or tissuespecific expression of RNA or proteins. Similarly, this system can beused to investigate constitutive and transient expression of the genesencoding the cDNAs of SEQ ID NOS: 1-327 and the proteins encoded bythese genes.

Further, the disclosed cDNA sequences can be used to determine thechromosomal location of the genomic DNA and to map genes in relation tothis chromosomal location. The disclosed nucleotide sequence can befurther used to identify additional genes related to the nucleotides ofSEQ ID NOS: 1-334 and to establish evolutionary relatedness amongspecies based on the comparison of sequences. The disclosed nucleotideand polypeptide sequences can be used to select for those genes orproteins that are homologous to the disclosed cDNAs or polypeptides,using well-established positive screening procedures such as Southernblotting and immunoblotting and negative screening procedures such assubtractive hybridization.

Method for Using Nucleic Acid Probes or Antibodies to Stage Embryos

Accurate staging of tree embryos is critical. It is known that differentstages of tree embryos have different capacities as subjects for genetictransformation and genetic engineering. In addition, environmentalrequirements exhibited by embryos vary due to increasing physiologicage. Currently, the staging of tree embryogenesis is most accuratelyperformed by an expert in the field who is very familiar with themorphological appearance of embryos at different stages. The cDNAs andrelated molecules of this invention can be used as markers for differentstages of tree embryogenesis, thereby eliminating the need for asubjective eye to assess maturity and potentially allowing for moreaccurate staging of tree embryos. Moreover, by monitoring the expressionof the underlying genes, it is possible to determine when an embryo hasreached a certain level of development even if that level does notcorrespond to a visible difference in embryo morphology. The relationaldatabase of this invention aids the ability to monitor expression levelsand tailor research approaches, such as the use of DNA arrays, to thespecific needs of the objective, i.e., staging.

The information provided in this invention can be used in whole or inpart to stage embryos. For example, one or a multiplicity of nucleicacid molecules from SEQ ID NOS: 1-327 having an expression profileconsistent with a particular embryo stage can be used in this invention.A researcher may find it beneficial to use oligonucleotide probes orantibodies, for example, that specifically recognize proteins derivedfrom genes expressed during middle embryonic stages, or thatspecifically monitor expression levels for embryos that have reachedmaturity associated with late developmental stages. A researcher canquickly determine that an embryo subset has progressed to or through anembryonic stage with the use of this invention and make appropriatechanges in conditions if necessary, e.g. alter growth media or otherenvironmental conditions.

Method for Monitoring, Enhancing, or Determining Expression ofStage-Specific Genes

Expression patterns of SEQ ID NOS: 1-327 indicate that gene activationcan be classified as stage-specific, such as in the case of SEQ ID NO:327, otherwise known as “LP2-3.” The promoter that drives such a genecan perform valuable functions. For example, a promoter from LP2-3operatively linked to a reporter gene presented within an embryo systemis expected to produce the reporter product under the conditions forexpression of gene LP2-3. Thus, the system allows a rapid determinationof stage specific embryos by a simple phenotypic reporter screen,perhaps by visualization of green fluorescent protein (GFP) or by lossof fluorescent protein product. Similarly, a set of promoters fromknown, differently staged genes operatively linked to reporter geneswill be effective for monitoring developmental changes within the systemas the embryos mature. The LP2-3 promoter is identified as SEQ ID NOS:328-334 in Table I. The promoter expression pattern is that of thenatively linked gene, LP2-3.

Virtually any indicator or reporter gene can be used for this approachor for other methods associated with this invention provided they arecompatible with the system studied. Generally, reporter genes are genestypically not present in the recipient organism or tissue and whichencode for proteins resulting in some phenotypic change or enzymaticproperty. Examples of such genes and assays are provided by Schenborn,E. and Groskreutz, D., Mol. Biotechnol., 13:29, 1999; Helfand, S. L. andRogina, B., Results Probl. Cell Differ., 29:67, 2000; Kricka, L. J.,Methods Enzymol., 305:333, 2000; Himes, S. R. and Shannon, M. F.,Methods Mol. Biol., 130:165, 2000; and Leffel, S. M. et al.,Biotechniques, 23:912, 1997, which are incorporated in their entirety byreference. In one embodiment of this invention, the reporter used isGFP, or any ariant of the fluorescent protein.

Additionally, one skilled in the art would recognize that a promoter,like that from LP2-3, has potential to stimulate production of productsnot ordinarily observed at a particular stage. A promoter derived from agene that expresses during a known stage, for example an early stage,can be operatively linked to a gene that does not normally expressduring that stage, yielding controlled expression of any targeted gene.It may be shown that earlier or later expression, or prolongedexpression of a particular gene may give a desirable genotype orphenotype in a mature plant, may result in increased vigor in culture,or may be sufficient to alter the normal maturation process of theembryo. Prolonged expression of any desired gene also may be achievedfrom linking a constitutively expressed promoter to the targeted gene.Further, the ability to manipulate gene expression during embryogenesisallows for a detailed study of the effects of an individual gene ormultiple genes on embryogenesis, leading to a better understanding ofthe developmental processes involved in embryogenesis.

Method of Correlatinq Gene Expression with Improved Tree Stock orCulture Conditions

Importantly, the cDNAs and related molecules of the invention can alsobe used as markers to examine genetic heterogeneity and heredity throughthe use of techniques such as genetic fingerprinting. These markers canalso be correlated with improved agronomic traits including goodinitiation frequency, embryonic maturation, high frequency ofgermination, rapid growth rates, herbicide tolerance, insect resistance,pathogen resistance, climate and environmental adaptability woodquality, and wood fiber quality and content, to name a few.Additionally, the expression of these developmentally regulated genescan be compared among genetically identical clones grown under differentculture conditions to determine the best protocols and media for somaticembryogenesis.

Cryogenic storage of pine tree embryos is effective for maintainingstocks of embryos determined by this invention to have the desiredfitness traits or exist at the appropriate developmental stage. Withsuch storage, one can specifically target desirable embryos forexpansion many years after they are frozen. For example, a culture ofsomatic embryos can be divided into at least three portions, one ofwhich is cryogenically stored, one which is used to study gene embryonicgene (and protein) expression, and one that is used to produce seedlingsfor field testing. Clones producing valuable mature plants could beselected and expanded from frozen stocks. Additional clones exhibitingsimilar expression patterns could be selected for future expansion andcultivation.

As will be evident to the ordinary practitioner, there are numerous waysin which the nucleic acids, polypeptides and antibodies of thisinvention might be used to characterize the gene expression of embryos.Ideally the stage-specific gene expression of embryos of severaldifferent genotypes and at several different stages of embryogenesis arecharacterized. For example, sets of oligonucleotide primers designedusing any one of SEQ ID NOS: 1-327 may be used in RT-PCR assays tocharacterize expression of a gene product. In situ hybridization assaysor antibody staining protocols may also be used to characterize RNAand/or protein expression and localization.

Embryos of the same genotype in which gene expression has beencharacterized may also used be to generate plantlets that are used infield testing. Once the embryos have developed into mature trees, thevarious genotype trees can be evaluated for important traits such asgrowth rates, herbicide tolerance, insect resistance, pathogenresistance, climate and environmental adaptability, wood quality, andwood fiber quality and content, among others. Finally the phenotypicdata collected from the field testing can be correlated with geneexpression during early embryogenesis to further enhance the database ofthe present invention. This will allow further identification of geneproducts which whose expression is correlated, either positively ornegatively, with commercially valuable tree characteristics.

It will be clear to those skilled in the art that identification of suchgene products can have several uses. Determining the correlation betweena desirable phenotype and a genotype would allow for the “pre-selection”of tree embryos for field testing. It would also be useful in evaluatingexperimental tissue culture conditions for somatic embryogenesis; inother words, the expression level of a gene known to correlate with thedevelopment of trees with desirable characteristics could serve as thecriterion on which culture media is evaluated, as opposed to assessingthe phenotype of fully matured trees. The ability to evaluate cultureconditions without having to develop fully mature trees and do fieldtesting would save a great deal of research time and expense. And ofcourse, the knowledge of the correlation between gene expression anddesirable tree phenotypes would serve to identify target genes forgenetic engineering.

Genetically Engineering Trees and Other Plants

There are several methods known in the art for the creation oftransgenic plants. These include, but are not limited to:electroporation of plant protoplasts, liposome-mediated transformation,polyethylene-glycol-mediated transformation, microinjection of plantcells, and transformation using viruses. Because the invention isespecially concerned with the transformation of woody species, the twoprevalent methods for transforming forest trees, namelyAgrobacteriurm-mediated transfer and direct gene transfer by particlebombardment, will be discussed in more detail, though it is understoodthat the present invention encompasses generation of transgenic plantsvia standard methods commonly known in the art.

Agrobacterium Mediated Transfer

A. tumefaciens and A. rhizogenes are two soil microorganisms thatnaturally infect a wide variety of plants including dicotyledonousplants, gymnosperms and some monocotyledonous plants. Infection by theseorganisms results in the growth of crown gall tumors or in hairy rootdisease, respectively. Each of these organisms carries a large plasmid,the tumor inducing (Ti) plasmid, in the case of A. tumefaciens and theroot-inducing (Ri) plasmid in the case of A. rhizogenes. These plasmidshave two critical features, a set of virulence genes and a segment ofDNA called T-DNA that is delimited by conserved regions of approximately25 base pairs known as the left and right borders. During infection, theT-DNA is transferred to the plant cell where it is able to stablyintegrate in single copy in the plant genome. Transfer of T-DNA requiresthe function of the virulence genes.

In its natural state, T-DNA contains genes that mediate progression ofdisease such as growth hormones or genes controlling root morphogenesis.Using recombinant DNA technology, however, T-DNA may be modified tocontain an expression cassette encoding a foreign gene of interest.There are several T-DNA vector systems commonly in use for thetransformation of plants. Several of these vector systems are reviewedin Hansen et al., Current Topics in Microbiology and Immunology 240:21-57 (1999) which is hereby incorporated by reference. T-DNA vectorsmust include the left and right borders. In addition they must either becapable of replication in Agrobacterium or be designed so as torecombine with a plasmid that does so. The latter type of vector isknown as a co-integrate vector. For transformation to proceed, theremust also be a source of virulence (vir) genes. The vir genes may be onthe same plasmid with the T-DNA or more likely supplied by a helperplasmid. For example, binary T-DNA vector systems are comprised of twoplasmids, one containing the vir genes and the other containing T-DNA.Some plants known to be recalcitrant to Agrobacterium-mediatedtransformation may be transformed if additional copies of some or allvirulence genes are provided. Extra copies of VirG and VirE can beparticularly useful.

Additionally, it is convenient to include in the T-DNA a selectablemarker that will allow identification and selection of transformed plantcells. The selectable marker should be one that works in bothAgrobacterium and the target plant. For example, the genes encodingchloramphenicol acetyltransferase and neomycin phosphotransferase aresuitable marker genes that confer resistance to chloramphenicol andkanamycin, respectively. Additionally, a selectable marker may beprovided on a separate T-DNA from the T-DNA encoding the gene ofinterest. Co-transformed T-DNAs can integrate at separate sites in theplant genome. This can be useful because it will later allow segregationof the marker gene in progeny enabling the generation of transgenictrees expressing the gene of interest but not the marker gene.

The gene of interest and the selectable marker genes must also be underthe control of promoters that function in the transformed plant cell.Examples of suitable promoters include, but are not limited to: theabscisic acid (ABA)-inducible promoter from the early methionine (Em)gene from wheat (Marcotte et al., Plant Cell 1:976-979 (1989); thecauliflower mosaic virus (CaMV) 35S promoter (Odell et al., Nature313:810-812 (1985); and the nopaline synthase (nos) promoter (Sanders etal., Nucl. Acids Res. 15(4):1543-58 (1987). Tissue-specific plantpromoters or plant promoters responsive to chemical, hormone, heat orlight treatments may be used. Additionally, the gene of interest may beexpressed under the control of its endogenous promoter to ensure properregulation.

The process of transformation requires plant cells that are competentand that are either embryogenic or organogenic. The plant cells to betransformed are then co-cultivated with Agrobacterium containing anengineered T-DNA vector system for 1-5 days. Following theco-cultivation period, the cells are incubated with the antibioticagainst which the selectable marker confers resistance, and transformedlines are selected for further cultivation. The use of Agrobacteriummediated transfer in woody trees is described in Loopstra et al., PlantMolecular Biology 15:1-9 (1990), Gallardo et al., Planta 210:19-26(1999) and Wenck et al., Plant Molecular Biology 39:407-419 (1999), eachof which is hereby incorporated by reference.

Direct Gene Transfer by Particle Bombardment

Direct gene transfer by particle bombardment provides another method fortransforming plant tissue. This method can be especially useful whenplant species are recalcitrant to transformation by other means. In thistechnique a particle, or microprojectile, coated with DNA is shotthrough the physical barriers of the cell. Particle bombardment can beused to introduce DNA into any target tissue that is penetrable by DNAcoated particles, but for stable transformation, it is imperative thatregenerable cells be used. Typically, the particles are made of gold ortungsten. The particles are coated with DNA using either CaCl² orethanol precipitation methods which are commonly known in the art.

DNA coated particles are shot out of a particle gun. A suitable particlegun can be purchased from Bio-Rad Laboratories (Hercules, Calif.).Particle penetration is controlled by varying parameters such as theintensity of the explosive burst, the size of the particles, or thedistance particles must travel to reach the target tissue.

The DNA used for coating the particles should comprise an expressioncassette suitable for driving the expression of the gene of interest.Minimally this will comprise a promoter operably linked to the gene ofinterest. As with Agrobacterium mediated transformation. Suitablepromoters include, but are not limted to, the the abscisic acid(ABA)-inducible Em promoter from wheat (Marcotte et al., Plant Cell1:976-979 (1989), the CaMV35S promoter (Odell, et al., Nature313:810-812 (1985), and the NOS:promoter (Sanders et., Nucl. Acids Res.15(4):1543-58 (1987).

Methods for performing direct gene transfer by particle bombardment aredisclosed in U.S. Pat. No. 5,990,387 to Tomes et al. Additionally, Elliset al. describe the successful use of direct gene transfer to whitespruce and larch trees in Bio/Technology 11, 84-89 (1993).

Researchers skilled in the area of DNA or gene transformation willrecognize that additional procedures, or combination of procedures, maybe useful for the successful tranformation of genetic stock.

Antisense Expression

The cDNAs of the invention may be expressed in such a way as to produceeither sense or antisense RNA. Antisense RNA is RNA that has a sequencewhich is the reverse complement of the mRNA (sense RNA) encoded by agene. A vector that will drive the expression of antisense RNA is one inwhich the cDNA is placed in “reverse orientation” with respect to thepromoter such that the non-coding strand (rather than the coding strand)is transcribed. The expression of antisense RNA can be used todown-modulate the expression of the protein encoded by the mRNA to whichthe antisense RNA is complementary. This phenomenon is also known as“antisense suppression.” It is believed that down-regulation of proteinexpression following antisense RNA is caused by the binding of theantisense RNA to the endogenous mRNA molecule to which it iscomplementary, thereby, inhibiting or preventing translation of theendogenous mRNA.

The antisense RNA expressed need not be the full-length cDNA and neednot be exactly homologous to the target mRNA. Generally, however, wherethe introduced sequence is of shorter length, a higher degree ofhomology to the endogenous mRNA will be needed for effective antisensesuppression. Preferably, the introduced antisense sequence in the vectorwill be at least 30 nucleotides in length, and improved antisensesuppression will typically be observed as the length of the antisensesequence increases. The length of the antisense sequence in the vectormay be greater than 100 nucleotides. Vectors producing antisense RNA'scould be used to make transgenic plants, as described above, insituations when desirable tree characteristics are produced when theexpression of a particular gene is reduced or inhibited.

METHODS

Tissue Samples

The cDNAs of the current invention can be derived from any sets of planttissue. The cDNAs of SEQ ID NOS: 1-334, for example, were originallyderived from embryonic tissues of pine tree embryos staged 1-9.9 asclassified in Pullman and Webb TAPPI R&D Division 1994 BiologicalSciences Symposium, pages 31-34, which is hereby incorporated byreference. LPS and LPZ clones are derived from somatic and zygoticembryos, respectively. As noted, embryos may be of either somatic orzygotic derivation, and the embryos may be grown in either semi-solid orliquid tissue culture systems. Applicable methods for growing embryos insemi-solid or liquid tissue culture systems are disclosed in U.S. Pat.Nos.: 5,036,007; 5,236,841; 5,294,549; 5,413,930; 5,491,090; 5,506,136;5,563,061; 5,677,185; 5,731,203; 5,731,204; and U.S. Patent Application60/212,651 filed Jun. 19, 2000, which are hereby incorporated byreference.

RNA Isolation

In one embodiment, RNA isolated from staged cell populations providesthe starting material for reverse transcription, differential display,and cloning of amplified cDNA. Methods and kits for isolating total RNAfrom cellular populations, or for generating poly(A)+ RNA, are commonlyknown in the art. For example, several procedures for isolating RNA aredisclosed in Chapter 4 of Current Protocols in Molecular Biology editedby F. A. Ausubel et al., John Wiley and Sons, Inc. (1987) (incorporatedherein by reference). As an example, the TRI Reagent7 available fromMolecular Research Center, Inc. (Cincinnati, Ohio) is a suitable reagent(used according to the manufacturer's instructions) for isolation of RNAfrom plant tissues.

Differential Display

Differential display provides a method to identify individual messengerRNAs that are differentially expressed among two or more cellpopulations. In the practice of the present invention, these cellpopulations may be provided by pine tree or other plant embryos ofdifferent developmental stages. The differential display procedure istaught in Liang et al., Science, 257:967-71 (1992) and in U.S. Pat. No.5,262,311, which are hereby incorporated by reference. Briefly, mRNAsequences are PCR-amplified using two types of oligonucleotide primersknown as “anchor” and “arbitrary” primers. Anchor primers are designedto recognize the polyadenylate tail of messenger RNAs. Arbitrary primersare short and arbitrary in sequence ard anneal to complementarysequences in various mRNAs. Products amplified with these primers willvary in size and can be differentiated on an agarose or sequencing gelbased on their size. If different cell populations are amplified withthe same anchor and arbitrary primers, one can compare the amplificationproducts to identify differentially expressed RNA sequences.

Cloning

PCR-amplified bands representing differentially expressed RNA samplesare excised from the gel, transferred to tubes and reamplified using thesame primer pairs and PCR conditions as used in the differential displayprocedure. Methods for the cloning of PCR products are commonly known inthe art and there are several commercially available reagents and kitsfor cloning PCR products. For instance, the pCR-Scipt™ Cloning kit fromStratagene, La Jolla, Calif.) is suitable for this purpose. Using thiskit, E. coli transformants containing plasmids with PCR fragment insertscan rapidly be identified using blue/white color selection followed byplasmid purification and restriction digests. The pCR-Script vectorcontains T3 and T7 polymerase recognition sites allowing for in vitrotranscription of the inserted fragment.

Sequence Determination

Methods for sequencing DNA, including cloned PCR products, are commonlyknown in the art. The selection of cloning vectors having M13, T7 or T3primer annealing sites flanking the PCR-amplified insert can be used insequencing reactions directly. Most sequencing procedures in use todayare modifications of Sanger's dideoxy chain termination sequencingreaction as disclosed in and Sanger et al., Proceedings of the NationalAcademy of Sciences, 74:5463-5467 (1977); which is hereby incorporatedby reference.

Homology Searching and Identification of Protein Coding Sequences

As understood by one of ordinary skill in the art, the sequence of acloned cDNA insert obtained, may be compared against public databasessuch as Genbank to discern any identity or homology to known sequences.Programs, such BLAST, for performing such a search are available on theNational Center for Biotechnology Information's web page located athftp://www.ncbi.nim.nih.qov. The results from Genbank search may revealthe potential function of a polypeptide or RNA molecule encoded by thecDNA. In addition to searching gene sequence database, the use ofcommercially available analysis software is well known in the art. Forexample, software packages such as the Wisconsin Package™ (GeneticComputer Group, Madison, Wis.) include programs such as FRAMES andCodonPreference that help to identify protein coding sequences in aquery nucleotide sequence. FRAMES displays open reading frames for thesix DNA translation frames, allowing one to quickly assess the presenceor absence of stretches of open-reading frames that are likely to beprotein encoding regions. CodonPreference is a more sophisticatedprogram that identifies and displays possible protein coding regionsbased on similarity of the codon usage in the sequence to a codonfrequency table (Gribskov et al., 1984).

EXAMPLE 1 Differential Gene Expression Analysis in Pine Tree Embryoenesis

cDNA libraries were prepared from staged pine tree embryos, as describedabove. The differential display technique was used to identify 327 novelcDNAs that were preferentially-expressed during early, middle, or latestages of pine tree embryogenesis, as set forth below. Clonenomenclature is divided into subsets based on tissue type; a clone isdesignated LPS to indicate somatic origins and LPZ for zygotic origins.

Plant Materials

Somatic embryos were collected at different stages of development.Cultures of somatic embryos of were initiated from Loblolly pineimmature zygotic embryos as described by Becwar et al., Forestry Science44:287-301 (1994) (incorporated by reference) or with minormodifications in media mineral composition. Somatic embryos were grownin cell suspension culture medium 16 (Pullman and Webb, Tappi R&DDivision 1994 Biological Sciences Symposium) and a maturation mediumsimilar to that of a standard maturation media. Resulting somaticembryos were selected and classified as stages 1-9 according tomorphological development following the teachings of Pullman and Webb,Tappi R&D Division 1994 Biological Sciences Symposium pp.31-34. Somaticembryos were sorted into tubes containing the same stages and stored at−70° C.

RNA Isolation

Total RNA was isolated from all stages of somatic embryos of loblollypine and grouped into early, middle, and late phases of development. Theearly phase is represented by a liquid suspension culture containingembryos of stages 1 through stage 3. Middle phase contains embryos ofstages 4 through stage 6, while stages 7 through 9 formed the latephase. 60-100 mg aliquots of staged frozen embryos were ground in 1.0 mlof TRI Reagent® Isolation Reagent (Molecular Research Center, Inc.), acommercial product that includes phenol and guideline thiocyanate in amonophase solution and extracted according to the manufacturer'sinstructions.

Reverse Transcription of mRNA (RT-PCR)

The total RNA was used as a template to synthesize single stranded DNAmediated by MMLV reverse transcriptase (100 U/μl). The method involvesthe reverse transcription by PCR of the mRNA with an oligo-dT primer(H-T₁₁G: 5′ B AAGCTTTTTTTTTTTG 3′) anchored to the beginning of thepoly(A) tail, followed by a PCR reaction in the presence of a secondshort (13-mer) primer which is arbitrary in sequence [AP₁ (5′ BAAGCTTGATTGCC-3′) or AP₂ (5′ B AAGCTTCGACTGT-3′)]. Reverse transcriptionand Differential Display were conducted using the GenHunter RNAimage Kit1.

A 19 μl reverse transcription reaction (10 μl sterile water, 2.0 μl 5×RTbuffer, 1.6 μl dNTP (250 μM), 2.0 μl anchored primer (2.0 μM), 2.0 μlRNA template at 100 ng/μl) was prepared for each embryo phase sample.The reaction mixture was heated to 65° C. for 5 minutes in athermocycler, cooled to 37° C. and paused after 10 minutes while 1.0 μlMMLV was added. The program was allowed to resume at 37° C. for 50minutes. The reaction was then heated to 75° C. for 5 minutes, cooled to4° C. and stored at −20° C.

Incorporation of Radiolabeled Nucleotides by PCR

Differential Display PCR was performed in a 20 μl reaction containing 2μl of the reverse-transcribed cDNA template; 10 μl sterile water 2.0 μl10×PCR buffer, 1.6 μl dNTP (25 μM), 2.0 μl anchored primer H-T 11G, (2.0μM), 2.0 μl 13 mer arbitrary primer (AP₁ or AP₂ (2.0 μM), 0.2 μl Taq DNApolymerase, and 0.2 μl α³²P-dATP (2000 Ci/mmole). The cDNA was amplifiedby PCR: 94° C. for 3 minutes, 40 cycles of 94° C. for 30 seconds, 40° C.for 2 minutes, and 72° C. for 30 seconds, followed by 72° C. for 5minutes. The reaction was cooled to 4° C. and stored at −20° C.

Differential Display

The PCR products were separated on a Stratagene (La Jolla, Calif.)pre-cast 6% polyacrylamide sequencing gel at 30 watts constant power forapproximately 2.5 to 3 hours. 3.5 μl of sample was mixed with 2.0 μl, ofloading dye and incubated at 80° C. for 2 minutes immediately beforeloading onto the gel. The gel was rinsed in water and dried. Dilute³⁵P-dATP with loading dye was spotted at the corners as alignmentmarkers and the gels were exposed to Kodak BioMaX™ autoradiography film.An exemplary gel is shown in FIG. 1.

Bands that appeared to be possible markers for phase specific geneexpression were marked on the film and aligned over the gel. The bandswere excised by cutting through the film. The gel pieces were scrapedfrom the gel and transferred to tubes and re-amplified using the sameprimer pairs and PCR conditions as used for incorporation ofradiolabeled nucleotides.

Cloning of DNA Fragments from Differential Display

The PCR products from the gel fragments were purified, polished, ligatedand cloned into XL 10-Gold Kan ultracompetent cells by heat shock withthe Stratagene pCR-Script Amp SK(+) Supercompetent Cell Cloning Kitaccording to manufacturer's instructions. The transformed cells werespread on LB agar plates containing ampicillin, IPTG, and X-Gal each at50 μg/ml. The plates were incubated overnight at 37° C. Plasmidscontaining PCR inserts were identified using blue-white colonyscreening. The presence of inserts was confirmed by digesting the cloneswith restriction endonucleases, Msc I and Nla ll, followed by standardDNA gel electrophoresis. Transformants representing early, middle, andlate phase embryos were sequenced using standard dideoxy protocols knownin the art with the T3 primer.

Sequence Analysis

All sequences were analyzed using a program-database pair search of theNCBI BLAST 2.0 server, blastn-nr, blastn-others ests, and blastx-nr. Ineach case, the query sequence was filtered for low complexity regions bydefault and entered in FASTA format. Other formatting options were setby default; alignment view-pairwise, descriptions-100, andalignments-50. Using these parameter settings, significant similarity toknown DNA, RNA, or protein sequences was found for several of thenucleic acid molecules of SEQ ID NOS: 1-334, for example, thosedescribed herein. (Alignment data not shown).

EXAMPLE 2 Characterization of Full Length LP2-3 cDNA Sequence

SEQ ID NO: 327, designated LP2-3, was first identified throughdifferential display with T₁₂MG and AP₁ primers (GeneHunter). Thedifferential display band appeared to be present only in liquidsuspension cultures of Loblolly Pine somatic embryos. The conditions formRNA isolation, reverse-transcription, differential display-PCR, and gelseparation/visualization for producing this band were all as describedin Example 1. Likewise, the band containing the original LP2-3 fragmentwas excised from the differential display gel, amplified, and clonedinto pCR-Script AMP SK(+) according to standard protocols known in theart.

Northern Hybridizations Demonstrating Early-Specific Expression

Northern analysis demonstrated that the LP2-3 differential display clonehybridized to an approximately 1.2 Kb mRNA from liquid suspensionculture embryos but was undetectable in late (6-9) stage embryo RNA.(FIG. 11) In general, LP2-3 is most highly expressed in early stageembryos in liquid culture. LP2-3 mRNA is found most abundantly in earlystage somatic embryos, especially for embryos grown in liquidmultiplication medium. (FIG. 12) Further, transcription decreasesrapidly as embryos are transferred to maturation medium (stage 3 andstage 4) and begin to mature. LP2-3 transcripts are virtuallyundetectable at stage 6-9 somatic embryos grown on maturation medium.(See FIG. 12) Additional studies indicate that LP23 mRNA is expressedzygotically, particularly in early stage zygotic embryos, but isundetectable in mature vegetative tissues. (FIGS. 13 and 14)Specifically, the signal intensity from liquid suspension somatic embryoRNA was about 3 times greater than the signal from the analogous stage 1zygotic embryo RNA. (FIGS. 13 and 14) LP2-3 transcripts were notdetectable in total RNA from needles, stems, or roots of one year oldseedlings, including those exposed to cold, ozone, wound stresses, orthe hormone jasmonic acid (not shown).

LP2-3 Differential Display and ‘Full-Length’ cDNA Sequences

A ‘full-length’ cDNA was captured from SMART™ cDNA made from somaticembryo liquid suspension by using a biotinylated LP2-3 differentialdisplay fragment as a capture probe. The “full-length” cDNA was clonedand sequenced according to standard protocols known in the art. Thissequence was designated at LP2-3⁺.

GenBank blastx searches conducted with the above sequence translated inall 6 reading frames indicated that LP2-3+likely encodes a member of themajor intrinsic protein family. This family of proteins encodes membranechannels for the transport of water and/or ions across cell membranes.They may play a significant role in osmoregulation and may play a rolein the cellular responses to water and salt stresses. As is known in theart, the MIPs are induced by dessication, flooding, and high levels ofthe plant hormone ABA. In contrast, the LP2-3 sequence was not detectedin desiccated late-stage embryos which have high levels of ABA and,thus, appears to be regulated by some embryo-specific signal.

EXAMPLE 3: Hypothesis Development for Improved Protocols

Currently the improvement of tissue culture practices arises viahypothesis, evaluation and adoption. Hypotheses arise from observationof size, shape, weight, etc. and physiological measurement of ion orsugar content (FIG. 6, box 1). These observations are limited in scopeand this limits the improvements that can be made to the tissue cultureprocess. Gene expression is closely linked to metabolic condition, thusthe observation of which genes are induced or repressed under a givengrowth condition, naturally, on the tree, or in a culture vessel,provides insight into the metabolic state of the embryo. Thisinformation can be used to create new hypotheses that can be evaluatedby modifying tissue culture.

To this end, mRNA levels of two cDNAs (LPZ-202 and LPZ-216), similar to“Late Embryogenesis Abundant” (LEA) proteins, identified in otherplants, were monitored. These genes are induced by the plant hormoneABA. Two peaks of mRNA were observed in these clones rather than thetypical single peak in most plants. (See FIG. 4 for clone LPZ-216; cloneLPZ-202 is similar but data is not shown.) It was subsequently confirmedthat two peaks in ABA activity are observed during development and thatthese correspond in timing to the elevation in mRNA for LPZ-202 andLPZ-216. Thus mRNA abundance profiles are providing insight into embryophysiology. (See FIG. 7) The effect of giving two pulses of ABA to oursomatic embryos is assessed; a tissue culture modification that we mightnot have considered as important had the gene expression data beenunavailable. Internal data shows fluctuations in the abundance of mRNAfor cDNAs listed in this collection (data not shown.)

Zygotic and Somatic Loblolly Pine Embryos

Loblolly pine cones were collected weekly from a breeding orchard nearLake Charles, La., and shipped on ice for experimentation. Embryos wereexcised and evaluated for developmental stage (Pullman et al. 1994).Stage 9 embryos were separated by the week they were collected-9.1 (week1), 9.2 (week 2), etc. Staged zygotic embryos were sorted into vialspartially immersed in liquid nitrogen and stored at −70° C. Somaticembryos for loblolly pine were initiated as described by Becwar et al.(1995) or with minor modifications. Somatic embryos were grown,selected, and staged as described by Pullman et al. (1994) and stored at−70° C.

cDNA Probe Preparation and Hybridization

30 ng of purified Lea protein cDNA fragments was labeled with ³²P dCTPusing the Ready-To-Go cDNA Random Labeling kit (Pharmacia). The labeledcDNAs were purified using NICK Column (Pharmacia) and heat denatured forhybridization. The RNA slot blot was pre-hybridized in hybridizationbuffer (0.5 M sodium-phosphate, pH 7.2, 5% SDS, and 10 mM EDTA) at 65°C. for 2 hours in a hybridization oven (Model 400, Robbins Scientific,Sunnyvale, Calif.) and the hybridized in the same conditions with thecDNA probes. After hybridization, the membranes were washed at 65° C. in0.2×SSC and 0.1% SDS. Each wash was 15 min. The membranes were thenexposed to Image Plate.

The probes can be stripped from the RNA slot blot by pouring boiling0.5% SDS onto the membrane twice and incubating without heating for 30min. The stripped blot was then exposed to Image Plate for overnight tocheck the completeness of the de-probing before next round ofhybridization.

To ensure the equal loading of the each RNA sample, the same membraneswere stripped and hybridized with a ³²P-dCTP labeled 26S ribosomal rDNAfragment. These results were used as controls to normalize the Leaprotein gene expression levels.

As a means of evaluating the usefulness of these arrays, we followed theexpression of three cDNAs that have strong sequence similarity to lateembryo-abundant proteins, (Lea) proteins from cotton (Baker et al 1988).Lea proteins and mRNAs appear in embryos at a stage when ABA is high andthe genes can be induced in vegetative tissue by application of ABA. Thetranscript level of Lea genes LPZ-202 and LPZ-216 showed two peaks,rising from stage 5 and returning to a base line about stage 9.2 thenrising again around stage 9.5. (See FIG. 4 for clone LPZ-216).

To confirm the fluctuation in lea transcript levels by Northernanalysis. RNA was extracted from zygotic embryos at different stages ofdevelopment A in ‘dehydrin’ cDNA from the North Carolina StateUniversity cDNA collection(hftp://www.cbc.med.umn.edu/ResearchProiects/Pine/DOE.pine/index.html)was used as probe for some experiments. Dehydrins are a class of leaprotein, originally identified as water deficit inducible proteins.Since the expression of this class of protein is well characterized, incontrast to our lea genes, the dehydrin expression profile could act asa reference point. After probing with dehydrin, blots were stripped andprobed with a 26S rDNA probe from Arabidopsis to check the loading ofthe original gel. The normalized expression pattern of dehydrin in thezygotic embryogenesis is illustrated in the top panel of FIG. 4. Theexpression of the dehydrin gene was induced at stage 5 and reached apeak at stage 6. It declined at stage 7-8, just prior to the onset ofthe desiccation. Then the mRNAs level remained low from stage 9.1through 9.5. The dehydrin mRNA levels rose again late in development,from stage 9.6 on, apparently dropping in very late development. Asimilar pattern of expression was observed in a parallel experiment whenour lea-like clone, LPZ-216, was used as a probe.

This pattern reveals two significant peaks at the early development ofthe embryos and high expression levels for the stage 9.6 and beyond. Theexpression pattern of these two lea genes in loblolly pine embryos isconsistent with the changes in ABA concentration observed in pine duringembryogenesis. (See FIG. 5)

EXAMPLE 4 Evaluation of Metabolic State of Somatic

Embryos as Compared to Zygotic Embryos for Fitness Determination

The model and goal for somatic embryogenesis is to produce an embryothat in vigor, germinatability, etc., resembles a zygotic embryo.Standard measurements reveal relatively little about the embryos; thusthe metabolic state of somatic and zygotic embryos is unknown. Themetabolic state of zygotic (natural) embryos can be evaluated by DNAarrays containing the cDNA clones described in this application. Adatabase of mRNA levels for the genes represented on the DNA arrays canthen be established. Embryos growing under a new tissue culture protocol(FIG. 6, box #2) can be evaluated by DNA array southerns (FIG. 6, box#3). The array elucidates patterns of gene activity and reveals whetherthose patterns are similar to the natural state (FIG. 6, box #4). Thegermination, or further development can be checked (FIG. 6, box #5) toconfirm the conclusion. Where a link between specific gene activity andembryo performance has been demonstrated, protocols can be modified withefficiency as seen in FIG. 6, box 6.

To illustrate this process, elevation of plant hormone ABA in maturationmedium was evaluated as a protocol modification, as described below.This modification proved beneficial, elevating the number and quality ofthe embryos produced. The mRNA abundance for cDNAs was assessed by DNAarray using RNA isolated from control and elevated ABA conditions;several differences were observed in the mRNA levels of specific genes.Further, abundance of mRNA in the elevated ABA condition, more closelyresembled the mRNA abundance observed for the these same genes inzygotic embryos. Thus a protocol which produces higher quality embryosproduces, in these embryos, a mRNA profile that more closely resemblesthat observed in natural embryos.

Zygotic and Somatic Loblolly Pine Embryos

Loblolly pine cones were collected weekly from a breeding orchard nearLake Charles, La., and shipped on ice for experimentation. Embryos wereexcised and evaluated for developmental stage (Pullman et al. 1994).Stage 9 embryos were separated by the week they were collected-9.1 (week1), 9.2 (week 2), etc. Staged zygotic embryos were sorted into vialspartially immersed in liquid nitrogen and stored at −70° C. Somaticembryos for loblolly pine were initiated as described by Becwar et al.(1995) or with minor modifications. Somatic embryos were grown,selected, and staged as described by Pullman et al. (1994) and stored at−70° C.

Mass Isolation of Genes Differentially Expressed in Loblolly PineZygotic Embryos

The following RNA differential display method is sensitive enough toproduce banding patterns from one mid- to late-stage embryo or 10-20early stage embryos. This technique, which extracts mRNA directly fromtissue using oligo(dt) beads, avoids losses inherent in conventional RNAextraction methods, is fast, reliable, and inexpensive. Differences ingene expression during development, as well as between somatic andzygotic embryos, can be easily detected.

To achieve these results, 50-100 μl lysis buffer containing 100 mMTris-HCl, pH 8.0, 500 mM LiCl, 10 mM EDTA, 1% SDS and 5 mM DTT was addedto 10-100 mg of staged embryos in a 1.5 ml tube. The mixture was groundthoroughly with an electric drill containing a plastic pestle bit (VWR,Cat# KT95050-99) that had been sterilized by autoclaving. An additional50-100 μl lysis buffer was added and ground briefly. The grinder andvortex was washed with 100 μl lysis buffer. If multiple samples wereprocessed, each is stored on ice until ready for the next step. Thegrinding tip was washed with sterile water and dried for the nextsample.

After all the samples were ground, they were spun at 4° C. for 15minutes in a bench top centrifuge at 14,000 rpm. 8 μl oligo(dT) coatedDynal beads (mRNA DIRECT Kit, Dynal, N.Y.) was placed in a 1.5 ml tube.The Dynal beads were washed twice with a 100 μl of the above mentionedlysis buffer and suspended in an equal volume of the lysis buffer usedin tissue grinding. If more than one sample is handled, the beads forall the samples can be washed together and dispensed in several 1.5-mltubes. The cleared embryo lysate (after centrifugation) was added to thebeads and mixed well.

The mixture was then incubated on ice for 5 min., placed on a magneticstand (Promega) for 5 min., and partially dried by careful removal ofthe liquid. To this, 100 μl of washing buffer with LiDS containing 100mM Tris-HCl, pH 8.0, 0.15 mM LiCl, 1.0 mM EDTA, and 0.1% SDS was added,(mRNA DIRECT kit.) The mix was transferred to a 200 μl PCR tube. Thebeads were washed once with 100 μl washing buffer with LIDS and oncewith 50 μl washing buffer containing 100 mM Tris-HCl, pH 8.0, 0.15 mMLiCl, and 1.0 mM EDTA. (mRNA DIRECT kit.) The beads were then washedquickly with 20 μl 1×RT Buffer (25 mM Tris-HCl, pH 8.3, 37.6 mM KCl, 2.5mM MgCl2, and 5 mM DTT) and 20 μl RT Mix containing 1×RT Buffer and 20μM dNTP was added. The tube was heated at 65° C. for 5 min. and cooledto 37° C. 1 μl MMLV reverse transcriptase (Promega) was added and themixture was incubated at 37° C. for 1 h. with occasional shaking. Next,20 μl of water was added to the RT reaction, mixed and a 1.0 μl to 20 μlaliquot of the PCR mix containing 1×Perkin-Elmer PCR buffer, 2.0 μMdNTP, 1.0 μM T12VN, 0.2 μM arbitrary 10-mer, 1 unit AmpliTaq(Perkin-Elmer), 50 μCi α³⁵S-dATP (Amersham) was taken. PCR usingtemperature settings of 94° C. 30″, 40° C. 1′, 72° C. 2′, 40 cycles, and72° C. 10′ extension was performed with the Perkin Elmer 9600 ThermalCycler. All PCR product was run on appropriate gels for bandvisualization.

cDNA cloning of Differential Display Bands

All dried gels were marked with radioactive ink prior to film exposurefor proper alignment between the X-ray film and the dried gel plate.Appropriate bands were marked by puncturing. A scalpel blade was used toscore the gel around each band to be excised. The excised gel pieceswere placed into a PCR tube containing 2 μl water. PCR was performedusing a 50 μl PCR mix (same as for differential display with thefollowing modifications: the primer concentration was 1 μM, and the dNTPconcentration was 200 μM; no α³⁵S-dATP is added.) The cycle settingswere the same as above.

A portion of the PCR products was run on a gel to determine amount andsize of PCR products; DNA that did not correspond to the size of theoriginal differential display band was discarded. The remaining PCRfractions were purified using CHROMA SPIN-100 columns (Clontech, PaloAlto, Calif.) according to the manufacturer's instructions. The purifiedPCR fragments were cloned into the pCR2.1 TA cloning vector (Invitrogen)according to Invitrogen cloning protocols supplied with the vector. Theonly variation from the standard protocol was an increase in the molarconcentration of PCR product to vector (over 100-fold); multipleinsertions were not found to be a problem. All ligations were performedat 16° C. overnight, transformed into E. coli strain DH5α, and platedonto LB with X-gal/IPTG.

Five colonies were chosen for PCR verification; PCR products of expectedsize were selected. About 10 μl of the 30 μl PCR reaction wassimultaneously digested with Nla III and Mse I overnight at 37° C. (a 5h digestion was used as well.) cDNA clones were selected according tothe colony PCR and the restriction enzyme digestion pattern.

The differential display protocol for finely staged zygotic embryos ofloblolly pine as described above, has produced more than 600differential display patterns and more than 60,000 bands. Within thatset of bands, we have identified bands that increased and/or decreasedduring embryo development. From those bands cDNA clones of thisinvention were isolated and sequenced.

Detection of Gene Expression by Micro-Array Assay

In order to verify expression patterns of the cloned DNA in loblollypine embryos a micro-array assay was developed. The cloned cDNAs wereamplified by PCR and adjusted to equal concentrations (0.1 μg/μl). ThecDNAs were then dispensed in the wells of a 384-well plate, denatured in0.3 M NaOH at 65° C. for 30 min. and neutralized with 2 volumes of20×SSPE mixed with 0.00125% bromophenol blue and 0.0125% xylene cyanolFF (5% gel loading dye). The denatured DNAs were then blotted on toHybond N+membranes (Amersham) as arrays using a VP 386 pin blotter (V&PScientific, Inc., San Diego, Calif.). Each DNA was dot-blotted fourtimes as a quartet on the membrane. An example of quartet spotting isseen in FIG. 7. Each dot is about 1.2 mm in diameter and contains about3 ng of DNA. DNA was then cross-linked to the membrane at 120,000 mJ/cm2in a CL-1000 UV-linker. (Strategene, Inc., Upland, Calif.) The dot imageof each membrane was scanned, numbered and saved in computer for lateruse in data digitizing.

The cDNA array membranes were pre-hybridized in hybridization buffer(0.5 M Na-phosphate, pH 7.2, 5% SDS, and 10 mM EDTA) at 65° C. for 30′in a hybridization oven (Model 400, Robbins Scientific, Sunnyvale,Calif.) and then hybridized under the same conditions with total cDNAprobes made from mRNA. The membranes were washed twice at roomtemperature in 2×SSPE and 0.1% SDS, twice in 0.5×SSPE and 0.1% SDS, andtwice in 0.1× hybridization buffer. Each wash was roughly 20 min. Eachmembrane was then exposed to Kodak Biomax MR films.

The total cDNA probes referred to above were made by initially creatingthe first strand cDNA. This was accomplished by mixing loblolly pineembryos (0.05-0.1 gm fresh weight) with 100 μl lysis buffer (containing100 mM Tris-HCl, pH 8.0, 500 mM LiCl, 10 mM EDTA, 1% SDS and 5 mM DTT)in a 1.5 ml Eppendorf tube. The mix was then ground with an electricdrill as described above. Another 100 μl lysis buffer was added and thelysate was ground again briefly. The drill pestle was washed with 100 μllysis buffer that was pooled with the lysate. After centrifugation at14K at 4° C. for 15 min. in a Beckman bench top centrifuge, the clearembryo lysate was mixed with 10 μl Dynal beads washed twice with lysisbuffer. The suspension was incubated on ice for 5 min., with occasionalmixing to allow binding of Poly (A) RNA to the oligo (dT) on the beads,and then left on a magnetic stand at room temperature for another 5 min.The liquid was removed and the beads were moved to a 0.2 ml PCR tube bysuspending in 100 μl lysis buffer.

The beads were washed twice with 100 μl of washing buffer with LiDS andonce with 50 μl of washing buffer. The mRNA was eluted from the beads in6 μl water at 65° C. for 2′. One μl T21VN primer (10 μM) and 1 μl SCSPoligo (cap switch primer, 5′-ctcttaattaagtacgcggg-3′, 10 μM) were addedto the mRNA eluate. The mixture was incubated at 70° C. for 2′ andcooled on ice. Three μl 5×First Strand Buffer, 1.5 μl DTT (20 mM), 1.5μl dNTP (10 mM each) and 1 μl MMLV Superscript II (Gibco BRL) were addedto the mRNA-primer mixture followed by incubation at 42° C. for 1 h tosynthesis first strand cDNAs. The cDNA was heated to 72° C. for 1 min.to degrade RNA and then diluted to 100 μl with water. The lysis buffer,washing buffer and Dynal beads are components of the mRNA DIRECT kit(Dynal, N.Y.). The first strand buffer (5×), 20 mM DTT and 10 mM dNTPare components of the SMART PCR cDNA synthesis kit (Clontech, Palo Alto,Calif.).

The first strand cDNAs synthesized as described above contains a T21VNsequence at their 5′ ends and the SCSP sequence (see “SMARTTM cDNA,Clontech, Palo Alto, Calif.) at their 3′ terminals. Total cDNA probeswere made by PCR amplifying the first strand cDNAs using SMART cDNA PCR(Clontech, Palo Alto, Calif.) in the presence of labeling agent. Five 5μl first strand cDNA solution was mixed with 5 μl 10×KlenTaq PCR buffer(Clonetech), 5 μl dATP+dGTP+dUTP (5 μM each), 1 μl T21VN primer, 1 μlSCSP oligo, 1 μl KlenTaq Mix, 5 μl ³²P-dCTP (10 mCi/ml, Amersham) and 27μl water. The PCR was performed using the setting of 94° C. 2′, 15cycles of 95° C. 15″, 52° C. 30″, 68° C. 6′. The PCR products werepurified using NICK column (Pharmacia) according to the manufacture'sinstructions.

Currently, high-density array Southerns for both somatic and zygoticembryos at all the developmental stages have been performed. The dotarray Southern data indicate that gene expression of late stage somaticembryos resembles middle stage zygotic embryos; many transcripts presentduring late zygotic embryogenesis (ZE) are absent in somatic embryos andlate stage somatic embryo gene expression patterns resemble the patternsof middle stage zygotic embryos.

Cairney et al. (In Vitro Cell. & Devel. Biol.-Plant. 36:155-162 (2000);Appl. Biochem. Biotech. 77-79:5-17 (1999)) have discussed how this geneexpression information may be used to improve the process of somaticembryogenesis; the rare incorporated in their entirety. As shown in FIG.2, the high-density array Southerns allows rapid evaluation of embryossubjected to protocol changes. Following the expression of a known genepermits inferences about metabolism and is very valuable in developingmedia-improvement hypotheses. Further, detailed gene expression studiesmay help by providing an understanding of the timing and location ofgene expression (e.g., in situ hybridization). The isolation of keygenes also provides the ability to monitor the expression of these genesas stage specific markers and allows protocol variations to be quicklyevaluated.

EXAMPLE 5 Identification of Markers for Superior Performance in TissueCulture”

The evaluation of tissue culture modifications for pine somaticembryogenesis, depicted in FIG. 8, is typically a lengthy process.However, where molecular tools are available, potentially improved mediaor genotypes can be discerned more rapidly, thereby avoiding the monthsof costly evaluation. (See FIG. 8) Table 5 illustrates this proposition.

Table 4 describes several publicly available clones. Lec. Fie, and Pkl,used to provide a representative model for this example. Any clonewithin Table 1, SEQ ID NOS: 1-327, can be substituted for those in Table4 to assay increased performance in tissue culture. Any promoter withinTable 1, SEQ ID NOS: 328-334, can be incorporated with those in Table 4or SEQ ID NOS: 1-327 to assay increased performance in tissue culture.In this scenario, Table 5, a representation of the information containedin FIG. 9, shows performance of selected genotypes (260, 480, 499, and500) in various media (1133 or 16) determined by the total number ofembryos produced per medium as described by Pullman and Webb (1994),incorporated herein. Embryo maturation was determined by the presence ofrecognized morphology according to methods previously mentioned above.(Pullman and Webb, (1994)) Genotypes that produced high, medium, and lownumbers of embryos were selected for RNA extraction. Gene expressionassays, such as DNA arrays, Northern blots, slot blots, etc., were usedin attempt to correlate embryo performance with mRNA abundance forselected genes. In the example shown in FIG. 9 and Table 5, expressionof loblolly pine genes, designated as Lec, Fie, and Pkl, obtained fromthe Pine Gene Discovery Project, was evaluated. The preliminarycorrelation appears to be that the high levels of the Lec gene's mRNAcorrelates with greater number of pine embryos. (See table 5.) Theseexperiments can be further expanded to incorporate additional oralternative genotypes with the prospect of identifying a largecollection of gene indicators of good or poor performance in tissueculture based on high or low mRNA levels. It is clear from the abovethat this approach, using the sequences disclosed in this application,can evaluate a genotype entering tissue culture, saving both time andexpense.

Somatic Embryos

Immature zygotic seeds were collected from loblolly pine genotype 260(mother tree BC-3, Boise Cascade). Somatic embryos were initiated asdescribed by Becwar et al. (1990) or with modifications in media mineralcomposition. The early stage somatic embryos were grown in cellsuspension culture medium 16 and sub-cultured every week (Pullman andWebb, 1994). The embryos collected from the suspension, which includestage 1 and stage 2 somatic embryos, are referred to as stage S embryos.At the end of the subculture week, the somatic embryos in the suspensionwere settled in a cylinder and transferred to maturation medium 240(Pullman and Webb, 1994). Resulting somatic embryos were selected,staged, sorted into vials containing the same stage, and stored at −70°C. until analyses were performed.

Probes

For the following example analysis RNA was isolated from embryos atdifferent stages in development, early stage somatic embryos andlate-stage somatic embryos. The cDNA probes used in this example are notcontained in the SEQ ID NOS: 1-327, but rather, are generic, publiclyavailable pine sequences obtained from the Pine Gene Discovery projectlocated at(http://www.cbc.med.umn.edu/ResearchProiects/Pine/DOE.pine/index.html).These clones are homologs to the well-studied Arabidopsis genes thathave been shown to have significant influence on embryo development inthis plant. The pine clone names (first column) and correspondingreferences for the Arabidopsis homologs are shown in Table 4. The threeclones listed, Lec, Lie, and Pkl, are for representative purposes withinthis example and it will be clear to one skilled in the art that any ofthe SEQ ID NOS: 1-327 could be substituted for those here as all willhelp identify conditions for improved performance in culture.

Probes were made by preparation of DNA using Wizard Minipreps (Promega,Madison, Wis.) and cDNA inserts isolated by restriction enzymedigestion. For the cDNA probes, 50 ng of the isolated cDNA insert DNAwas used to make ³²P-labeled probes with Ready-To-Go DNA labeling beads(Amersham Pharmacia Biotech) according to manufacturer's instructions.Blots were prehybridized (7% SDS, 1% BSA, 0.25 M NaPO₄ (pH 7.2), 1.0 mMEDTA) for 3 hours at 65° C. and hybridized in fresh buffer at 65° C. for12 to 18 hours (4). Each blot was washed 6 times with the followingconditions: 1) RT, 2×SSC, 0.1% SDS, 15 min; 2) RT, 2×SSC, 0.1% SDS, 30min; 3) 42° C., 0.2×SSC, 0.1% SDS, 15 min; 4) 42° C., 0.2×SSC, 0.1% SDS,30 min; 5) 60° C., 0.2×SSC, 0.1% SDS, 30 min; 6) 60° C., 0.2×SSC, 0.1%SDS, 30 min. Blots were exposed to a phosphorimaging plate for 10minutes. Screens were read with a BAS1800 (software v1.0) and imageswere manipulated with ImageGauge (v2.54) (Fuji Photo Film Co., Ltd.,Kanagawa, Japan).

The hypothesis tested within this example is that genotypes that producelarge numbers of embryos have high Lec expression and low Pklexpression, poor genotypes have the opposite pattern, and that Lec andPkl expression act as indicators of embryogenic potential. FIG. 9 showsthat Lec is not expressed in late stages of embryogenesis in somaticembryos. The Lec gene is expressed throughout embryogenesis inArabidopsis. The blot reveals that the Lec gene is a useful earlyexpression marker for embryogenesis. One interpretation of these resultsis that the somatic embryos do not express Lec in the manner that Lec isexpressed in zygotic embryos, i.e. the use of Lec expression hashighlighted a defect in gene expression in somatic embryos. This defectcould be used to identify desirable genotypes, i.e. those likely toprogress through development and produce a large number of healthyplantlets compared to undesirable genotypes that will cease developmentprematurely or produce low numbers of plantlets. This is an example ofthe principle described pictorially in FIG. 8.

The results described in the previous section of Example 5 reveal waysin which gene expression analyses can be used to improve somaticembryogenesis based on several genes. However, this principle applies aswell when the assay is expanded to determine the expression of hundredsor thousands of genes simultaneously (e.g. by DNA arrays). We can createhypotheses which state that expression of a single specific gene can beused to determine the potential of a culture, or hypotheses that statethat the expression of a group of genes (e.g., hypothetical genes A, B,C, D, E, F) acts as an indicator of high embryogenic potential. Forexample, all these genes may be expressed at a high level in cell linesthat produce large numbers of embryos, thus we would select cell lineswhich exhibited this characteristic. Alternatively specific levels ofexpression for genes A, B, C, D, E and F may be required and acombination of high and low expression of particular genes will identifydesirable cultures. Alternatively, experience will determine thatcertain exceptions can be tolerated.

While the previous paragraphs discuss numbers of embryos produced, theprinciple applies to ANY desired characteristic: by establishing acorrelation of gene expression with e.g., germination potential, embryosize, growth of plantlets in their first year, disease resistance ofmature plants, environmental hardiness or wood quality. Any trait wherecould be evaluated by these gene expression assays and correlations withgene expression established, resulting in a molecular tool which couldbe used to predict desirable characteristics. Explicitly, we could usethese gene expression tools to select cell lines which will produce highquality plantlets months before they grow into plantlets, or cell linesor juvenile plantlets which will produce hardy trees with desirable woodquality, years before these traits are expressed. TABLE I Embryo cDNAPhase Clone Nucleotide Sequence SEQ ID NO:1 Late LPS-001GGTACTCCACCGTAATAACCCTTGGGAAATAGCCTATGATCCAGGGGAGGCAACCACCTATATCATTGACAACAGCGAAAAATGTGGCGCAAGAAGTTTCACATACAATTCATGGTTACAAAGATCACATACCAGGTGTTGGAGCAGATTCGATAGATATTGAAGATATGAAGCCAAGGAGTGGAGCAGTTATTGAAAAGGGCACAAAAAAATTTGCCATTTACAAAGATGAAAATGGGCTGATTCACAAATACTCGGCAATATGCCCACACATGAACTGTATTGTGAAATGGAATCCTATAGACTCAACTTTCGATTGCCCCTGCCATGGTTCAATGTTTGATAATCTGGGTCGATGCATCAATGGACCTGCCAAGGCGGACCTATTTCCCGAAGATTAACGATAGTTGTTTGTACATGTAATTATCTTGATATTGTATATATATGTATTTAAATTATACAGTACAATAAATCCATGTTGCAGGCTATTTCTGCTTGATAATTTAGCTCCAGATTATACATAACCAGTTATTGGCTGTTTTCCCCTGGCAAAAAAAAAAAA SEQ ID NO:2 LateLPS-003 003GGTACTCCACAGAAAGAAATGATTTGACAGAAAAAGAGAGCTGTAGGATTGGGAAACCCTGCAGTGGATATATACAATGTATATGTACTCTGTCTGTTTTTCTGTTATTTGACGGAAATAAAAACGCCATAGCGACGGATGACTGTAAATCCTTAGGGACGGATGACTGTAAATCCTTAGGTTGGAAGATTACAAACGACATATGGGTCTTTCAATTTTCAGATTTCTGTAAGACTTACATTTCAAAGACTGTTTGGATGGGCAAAAAAAAAAAA SEQ ID NO:3 MiddleLPS-004 GGTACTCCACCAGAATGCCGCAGTTTAGTTCTCTAAAGCAAGCAGTAAATTAATTTTGTCAAAATCTAAAGAGTGTATAGTATCAGTGGGTTTGTATTTCCTAGTTTGCCTACAATAACGATGGGGATTCACCAGTTTTTGTAGAATTTGCAATCATCGGATGACAATTTCAAAGTTTTCTCTAAGTCACCCGCATTGATATCGAGAAGCCTTCCATTTTCAATTATTTAATATCAGAAAATCTTTTCAGTTGGCAAAAAAAAAAAA SEQ ID NO:4 Middle LPS-006AGCCCAGCTGCGAAGGGGATGTGCTGCAAGCGATAAGTGGTAACGCCAGGTTTCCAGTCAGACGTGTAAACGACGCCAGTGATGTATACGAATCACTATAGGCGATGGCCTTCTAGATGCATGCTCGAGCGCCGCAGTGTGATGAATTGCAGAATCGGCTGGTACTCACGGGCTAGAGAAAGGCACAAGCACTTTTTGTCATTTTAGGATCAGAGGCATTCAGGTATAGGAAGGGTGGCTCAGATAGGCAGATGGATCGGCATTTTGCCCAGTCATGAAACATTTTATGCATGTTATTGCCTCCCAAGGACGAAATCAGTTCTTTGTGCCTTCTGGTGATATCACTTCAAACAAAAGGCAACAGTTCTGTGATTTCATATGGTTTGTCACTGAATATTTTGTTGCAGATGTTCTCTACTATTTTTTATCTGCTTTCAAGTGATTATTTGTTGATTCCCCATGGATAGTTATGCTAATCAGTTGCATTTCTCTTGTACCAGTCAACAAACAAAAATGCTTGTAGGAATCCATTACTATTTATTTTCAGACAGGTAAACGTGTAGCTAATTGTTCTGGCAAAAAAAAAAAA SEQ ID NO:5 Middle LPS-007TCCAAAATACAAAGGCTTTATTTGCATCATGATATAATACAAAGTAAGAAATTTACCCAACTGTTTAACCTAATAATAATACAAAGGAAGCATTTTACCCAACTCTTTAACGTAATAATACCAAAGAGTGGAATGCTTTATTGACCAGCAAGACCTTGAAATTTTTATAACCAATGCCCATCAACAGAGCCTTTCTTAAAAAACGCAAAGCCCAGCTCTGTCACCTTATTAGTTAGTATAAACTGACATTCTTCCAAGCTTGTGTGCGCAGAAACAATAAAGAACTCACCTTGGTTTAAAGAACGTGCCATGAAGAAAACGTCCCAAGAAAAATGAAATGGCTCCTTCGACCATTCAGTCCTCCCTAGAAAAATCAAAAGACTCCTTCGACCATTAGGTCCTCCAATTGGGCATCTAACTACAAGCGGTC SEQ ID NO:6 Middle LPS-008GGTACTCCACGGGCTAGAGAAAAGGCACAAGCACTTCTTCGTCATTTTAGGGATCAGAGGCATTCAGGTATAGGAAGGGGTGGCTCAGATAGGCAGATGGATCGGCATTTTGCCCAGTCATGAAACATTTTATGCATGTTATTGCCTCCCAAGGACGAAATCAGTTCTTTGTGCCTTCTGGTGATATCACTTCAAACAAAGGCAACAGTTCTGTGATTTCATATGGTTTGTCACTGAATATTTTGTTGCAGATGTTCTCTACTATTTTTTATCTGCTTTCAAGTGATTATTTGTTGATTCCCCATGGATAGTTATGCTAATCAGTTGCATTTCTCTTGTACCAGTCAACAAACAAAAATGCTTGTAGGAATCCATTACTATTTATTTTCAGACAGGTAAACGTGTAGCTAATTGTCTGGCAAAAAAAAAAAA SEQ ID NO:7 Middle LPS-010ACGACGTGTAAACGACGGCCAGTGATTGTATACGACTCACTATAGGGCGATTGGCCTTCTAGATGCATGCTCGAGCGGCCGCAGGTGATGGATATCTGCAGAATTCGCTTGGTACTCCACGGCTAGAGAAAAGGCACAAGCACTTCTTCGTCATTTTAGGATCAGAGGCATTCAGGTATAGGAAGGGTGGTCAGATAGGCAGATGGATCGGCATTTTGCCCAGTCATGAAACATTTTATGCATGTTATTGCCTCCCAAGGACGAAATCAGTTCTTTGTGCCTTCTGGTGATATCACTTCAAACAAAAGGCAACAGTTCTGTGATTTCATATGGTTTGTCACTGAATATTTTGTTGCAGATGTTCTCTACTATTTTTTATCTGCTTTCAAGTGATTATTTGTTGATTCCCCATGGATAGTTATGCTAATCAGTTGCATTTCTCTTGTACCAGTCAACAAACAAAAATGCTTGTAGGAATCCATTACTATTTATTTTCAGACAGGTAAACGTGTAGCTAATTGTTCTGGCAAAAAAAAAAA SEQ ID NO:8 Middle LPS-011GGTACTCCACGAAGCAAAAAGAGTCAGGGGAATGAAGATGGGGGGCTCCGACAAGAAGCGGATCAGAGAAGAGCAGGAAATGAGTCCACCTGAGGAATCCTGGAGACAGAAACAGGGGCGTTTTAATGGAGTTTGAGGCAGGGATGGCCTATGATAAACCTGAAAATGCCGGTGCAGGTAATGAGAATTTGCCAGAGTTTTGCTCTCTTTCAAATGAGTACTCGATGTTATTGAAAGATCCATGGAGTTGGGAGGATAGCACTGGTTTCGGAATCCGAAGCTTAGCTGCTGTCAGGAAGCAGTCTTGTATATTGGACTATCTCCATGATTCTGCTGTAGATAATCGCTGTGAAAAGGATTTTGCCGAGCAGCACAAGGTACAGGAAGAGGAGGATTGTTTGAGAAGGTCTCTTTTTGAAGCCACAGATGATCAGCTCTGGAGGCTTCAGAGTCTTTGCAGGATACAGAAGGTCTGTTTCCTCTGGATTCCGTGGGTAGCCATGATTGCACGACCTTGTTGCAGGATGAGAGCATTGTTCAGGGCGCTGCTCTTACTTCAGAATTTGGGAACAGGATGATGGTCACAAGGATGCCAAAATTCATGAAGATGGCATTGGTTTTGTGTATGGGAGTGGGATCTCGGATTGGATTCGGAGGGCTCCCTCGAATCAATCTGAGTTTTCTGAATCTGTTGAATTTGAAAGCTCTATGTTTTCACTGTAATTTGGGTCTTTTTAATTTCTTCCTATGTAATTTGGGTGTTTCTAATTTCTTCCTTCAGCAA AAAAAAAAAASEQ ID NO:9 Middle LPS-012GGTACTCCACCATATCCAGGTAACAAGGGAAAACAGAGTCAGCTTCTAGTATGTTGTATGCCTTGCTCTGTCTGTTTTCTTTGATCTTTGATGCCAAGCAAGTTGAATGTGATCACTAAATGTTGCTGGCAGTAGAGCTGGAGATGTGCTGTCTCTTTGGTGTCATTAGCACAGAAGCTATTGGAGAAATGATTATTATCTGTTTGATAACTTCTAGAGCATTTTTCTGCTTCCAATTCCACAAGGTGGAAAGTGCAAGGATGTTTACTTTCTTAAACTGTACTTGCCTTGTATTTGATGATGTAAGGTTGTGTGGCAAAAAAAAAAAA SEQ ID NO:10 MiddleLPS-013 GGTACTCACCATATCCGGTAACAAGGGAACAAGTCAGTTTTAGAAAGTGGACCCCCGGTTCCGTCGTTTTCTTGATCTCGGAGCCAAGCAAGTGGATGTGATCACTAAATGTTGCTGGCAGTAGAGGTGGAGATGTGCTGTCTCTTTGGGTCATTAGCACAGAAGCTATTGGAGAAATGATTATGGTATTCCACCATATCCAGGTAAACAAGGGAAAACAGAGCTCAGCTTCTAGTATGTTGTATGCCCTGCTCTGTCTGTTTTCTTTGATCTTTGATGCCAAGCAAGTTGAATGTGATCACTAAATGTTGCTGGCAGTAGAGCTGGAGATGTGCTGTCTCTTTGGTGTCATTAGCACAGAAGCTATTGGAGAAATGATTATTATCTGTTTGATAACTTCTAGAGCATTTTTCTGCTTCCAATCCACAAGGTGGAAAGTGCAAGGATGTTTACTTTCTTAAACTGTACTTGCCTTGTATTTGATGATGTAAGGTGTGTGGCAAAAA AAAAAAA SEQ IDNO:11 Middle LPS-014GGTACTCCACCATATCCATGTAAACAAGGGAAAACAGAGCTCAGCTTCTAGTATGTAGTATGCCCTGCTCTGTCTGTTTTCTTGATCTTTGATGCCAAGCAAGTTGAATGTGATCACTAAATGTTGCTGGCAGTAGAGCTGGAGATGTGCTGTCTCTTTGGTGTCATTAGCACAGAAGCTATTGGAGAAATGATTATTATCTGTTACATAACTTATAGAGCATTTTTCTGCTTCCAATTCCACAAGGTGGAAAGTGCAAGGATGTTACTTTCTTAAACTGTACTTGCCTTGTATTTGATGATGTAAGGTTGTGTGGCAAAAAAAAAAAA SEQ ID NO:12 LateLPS-015 GGTACTCCACTAGACCGGGTAGGGTCTCTCCATGGTTTTGCGACTAGGTTAGGTGTCCTGTTCTGTTAATGATTTTGAGGTTTTGTTAATTGTGAGTATGTTTCCAGGGTTTTGAACCTGGGTACTCGGCCTTTGTTGGAATGTAGTCTGGTTAATTTATATGTATATGTAACCTTGGGGTTTCGAGCCCAGTTCTCTGTTCTTCTTGAAATGAAATGCGATTTGTT CTAAAAAAAAAAAASEQ ID NO:13 Late LPS-019ATATATACGTATGGTATTCCACAGCATGAACTCTCGACATTATATGCTTGTTATAGTTTTTAAGAGAGGAGACTTACCTCACACATGTACAGCTTTTTATTGTCGTGCTTTCAGTTGATGGATGATTGTTGTAGTCCTGTCATTGGTTGGACAATTTCATCATCCTAAAGATCCAAGAATTCATGTGGCAAGAAACTTTAATAAAGTCAAATATAATCCGATGACGTAACCCTAAAAAAAAAAAA SEQ ID NO:14 Late LPS-020GGTACTCCACTAGTGATCGATTCTCTGTATGTGACGCTGCGCGGCGGCTATAGCGCTTCACTGAGAATGTACGGTATATTATGATTGATGTGATGGATTTGCTCCGCAGCTTCGGCTGTTGTATCTGCTCACTTCGGCGTATATATGTAATATGTTGCTTCTTCAGAGAGATGAACTTCCCCCTAAAAAAAAAAAA SEQ ID NO:15 Middle LPS-023ATAGATCATTTTAAAGTTTCAGTGATTTGAATCTAATTCCACTGCATTTCCTCGCAAACTGGCAGTCAAATAGTATTCCCTCTTTCAGTGACAGGCTGGCAGGTGTTCATTCTTATACAAACATGATTATCATAATTCCATTAATTCATGGCGTTTTCTTTGCCAAAAAAA AAAAA SEQ IDNO:16 Late LPS-024TTTTTTTTTTTTAGGGAGAAAGGTAACTTCAGCCAGCTTTCAAAGGCAACACCTACAAAAGGGGTGACTGAGAACTCAGACACAGACGACAAGTGATCATTCGGGCCAGATTTTTGTTGAGAGAGTTGTAGTGTGTAATTGATTCATTTCATACATTTGATATGCAAGCCTGTACAATAGCCTGTGACTGTTAAGGGCATTCTTTTGTCTCCCTGTTGCTATTTGGGTTTCCGGTGTGTTCATTTTCACTTATTTTTGTGTTTTAGCTGGAAGAATTTGAGAGGGTAGAATTGTGTCATCGCTATGGCTTGTGCATGACTCATGAGCCAGCAGTTGAAACTTTTATTTATTAAGTTATAATACTATGTCTTGTCAATTCTCAATAAAAGATATTTTATGCTGTTGGGCAGCATCTAAAATGTTTTGTATGTTAGCATAAAATCCCATTTTCTATAAGTTTTTGCCAAAAAAAAAA SEQ ID NO:17 All LPS-025AGCAGGTTCAGTCAGACGTGTAAACGACGCCATGATGTATACGAACTCATATAGGGCGATTGGCCTTTAGATGCATGTTGACGGCCCGCAGTGTGATATTCGCAGATCGCTTTTTTTTTTTTTAGGCATGGTGCGCGATGAGCTGATAGCGATGATGAAGACCAAGACCACCAAAGGAAGATTCTTCAGAGCAAAAGCTACGGAGACAGAACCAGAGGACTCAAAGCCGGAATCCATTGGTGAGGTACCTGCAAATGTGTGATGGACTAACTAAGAAGGCTCCTTGAGAGGACCCATTAAGCACAGTGTTTTAGTCCCAAATTCTGTGCAATTCCGTTGAAAATCATTTTTACGATTTTAGGTATGATGTGTGCAATTTAAAGTTGGAATTATTGTGGGCAAAGGCTATAAGTGATTGTCTAATCCATTTAATTTATTATCTTTTGACTAAGAGCATATCTAGGCTGGAAGAAATTAGGGCACATATGTTTTGTGAATTTGAACATTCTGGGTTTTGCAATGCAAAACACCACAAATATTTTATAATGTAGAGGTGTACTTTTTCTGGCCAAAAAAAAAAAA SEQ ID NO:18 Middle LPS-026GGTACTCCACCAATAATACTGTCTGTTCTGCTCCCTGCTGATCCACTAAGCAGATTATTTCTGTCCACCCCACTTTAGAGTCTCAGTTTGTAAAGCACTCCCTAGGAGCTAAACTCATTTCCAATGGATTAAAGCACTCCATAGGAGCTAAACTCATTTCCAAGGGATTTTTGTCCATTTCTCTGTGCTAAAAAAAAAAAA SEQ ID NO:19 Early LPS-027ATGTATACATATATGTGGTACTCCACACACTCAATAACAGCATCACAATCAAAACAAGAAGGCGGCCAGAAAGCTTTAAAATGCTAAGCCTACAGGTAATATTCACAACTGCATTAAGCACCCCGCTCCTAGTTCTGAAGAAGCCAGAAAGCTTTAAAATGCTAAGCCTACAGGTAATATTCACAACTGCATTAAGCACCCCGCTTCCTAGTAGGCTAGTACTAGGACTAGGACCGCATTACCAGTTCCCTTATCTTCTACTCATCCTCTACAGGAAAACTATGACTAAAACTGCATTACCAGTTCCCTTATCTTCTCAACTCGTCCTCTACAAAAAA AAAAAA SEQ IDNO:20 Early LPS-028GGTAATTTCCACCCACCACGGGCTTTTTCAATTAACCCATTTCTACCACTCCACATAGGGTTCTAAGTTTTGTGACTCACCCCCAATTTCGCTGATATTTTGCATTGCAGCTGTTTATCTACAGGAAATGGCTAATCAGTACTTTCAGAATTGGTTGCTTCTGTACAGGAAATGGATAATCAATCAGTACTTCTATACTAAGTTGCTTACGCGGGGATCAGAGCCTTACTTCAGAAAATTGAATACATTTTCTTCTTTGTGTATGTATCAGGCATGGAATATATGTAGCATGCCATGGAATGCGTATTTACTAGATTATCTTTTAATTTAATACATATGTTGCTTACTAATTTGTCCACAAAAAAAAAAAA SEQ ID NO:21 Early LPS-029GGTACTCCACACACTCAAACAACAGCATCACAATCAAAACAAGAAGGCGGCCAGAAAGCTTTAAAATGCTAAGCCTACAGGTAATATTCACAACTGCATTAAGCACCCCGCTTCCTAGTTCTGAAGAAGGCCAGAAAGCTTAAAATGCTAAGCCTACAGGTAATATTCACAACTGCATTAAGCACCCCGCTTCCTAGTAGGCTAGTACTAGGACTAGGACCGCATTACCAGTTCCCTTATCTTCTACTCATCCTCTACAGGAAAAACTAGGACTAAAACTGCATTACCAGTTCCCTTATCTCTCAACTCGTCCTCTACAAAAAAAAAAAA SEQ ID NO:22 MiddleLPS-030 GGTACTCCACTATTAGATTGATGCAAGACCAACTGATCATGGCTAGGGTGTATCAAGCATTTCCCAGGCTAGGAATAATCTTGATTTATACCATGAATGATGCTTCGTATTAAAGAATGTCAACGTACATGGGTGAGACTAATGCCGATCTGATCTACCTCAAGGTAATAATTTTTGCATTAGCTGCTTCTAATCAAGAGTAGTAAGTGCTTCCATTTGC AAAAAAAAAAAASEQ ID NO:23 Middle LPS-031GGTACTCCACAAGGCATATATGGGCAATTGATTTTGCCTAGCCCAAATCCTATCAAGCTTGCGTATTTCTAAAAGATGCACTATTTTTTGTCCGAGTGTAGGTTTTGAATTCATTGTAACATTCAGCAATATTAATTCAGGGGTAGCATTTCTGGCAAAAAAAAAAAA SEQ ID NO:24Middle LPS-032TTTTTTTTTTTTAGGGTAGAAAACCATGCTTCACTAACAAGGTATTAAAATTACAATATAATTCTGGGTGTAAACGACCTGATAGATGATCTGCAAGTGCCAGGAGGCAATATCTAGCAGAATACGTACAAATAAATTGCCAAAAAAAAAAA SEQ ID NO:25 Late LPS-036GGTACTCCACCAATGATCACCCATGTCCATTTGGTTAATTCAATGTCAAGATTTAGTAGTTCCGTATTCCCTTGGGTAAGCTGTAATGGTCCATTTGGGAACAGTCCATGTTTGGGACACAAGTTCAATAGAGATGTCATCCATAAATATCCATAAATATGGGTATGAATCTCTTCCTCCCTCTCCGCCCAATAATAAAAAAAAAA SEQ ID NO:26 Late LPS-037TTTTTTTTTTTTAGTAGCAATAGCAATCCATTTTAGGGATCTGCAGATCAGTGACTAAGTGACCCCTACCCCCAAAGGATTAATTGTACTTTGGCTTAACCACAAAACCTGATCAATGTGAAGTTTTTACCCATATTAATTCCCAAAAGTAACTACAAATTCCAGAGTACATTTTTACCCAAAAAAAAAAA SEQ ID NO:27 Middle LPS-038GGTACTCCACTATACAATATCAAGGCATATCTGCCGGTGTGAATCATTCGGATCTCAAGCACTCTCCGTGCCGCAACTTCTGGCCAGGCTTTCCCTCAATGTGTGTTTGACCACTGGGATATGATGGGATCTGATCCATGGAACCTGGTCCCAAGCTGGGCAGCTTGTGACTGATATCCGTAAGAGGAAGGGTCTTAAGGAGAGTATGACTCCCTGTCAGAGTTCGAAGACAAGCTGTAGAGCTTTGCTATGTTTGCATGTCGGATGCTGTCAAGATTGAGGAACCTCCGAGTATTAACACAGTTTTGTGTGCTAGGACTAAATTTATGCTATTCACGTATTTTTGTGATCTGTATTTATGTTATCACGTATTTTTGATTGGAAAATACTTTTTACAAGTCATCCATTAATCTTTTAAATGTTACATAATTCTCTCTGT C SEQ IDNO:28 Late LPS-040AAGCTTGGTACCGAGCTCGGATCCACTAGTAACGGCCGCCAGTGTGCTGGAATCGGCTTGGTACTCCACTATACAACATCAAGGCATATCTG SEQ ID NO:29 M,L LPS-041CTTTTCTTCGTGCTTTCGTGGAGTACC SEQ ID NO:30 Middle LPS-042GGTACTCCACAAAGTGAGATGAGTGATATGAGGTCAAACACGTAAATGACAATAGCTATTATTTCCCCACTTGTTTGTGGCTGTGTATATTATACTTCATTGTCAGGACTTTTGTATGGTTGAAGTTGCAAGGTTTTGGCAAAAAAAAAAAAAAAA SEQ ID NO:31 Middle LPS-043GGTACTCCACCTCCAGCTGCTTATCCAAGTACTACGGATAGTTCATACTCCTATTATGCTTCTGCCAAGTGAACCAGAAGGCTTCTGTTCTACACTAGCAAACTGATAGCTCGAGCATCTCATTTACTAAGGATGATAATTCAAAATTGTAACATTGCAAACATCAGCAAACATCAGCATCAACTCTGTTACTATACAAGCAATGGATGCGTCGCTGATGCTGCGGGAGAGTAAATTTTTAGTTTACTGCGGTTGGTAATTGAGTAGGTTGACTTACATTTCTGTTGTAAAGCCGTTGTCGGGCATTGTTTATCTGGCCGAGTTAGCGCCAGGAAGCTAAATGTACCAAATATTTATTATTTTTATTAAGAATATAAAATTTAGTCGTCTTCTGCTGCCCAAAAAAAAAAAAAAAA SEQ ID NO:32 Late LPS-044ATGGCCATGGACTTATGACTTTCAAAACCCTAAAACCTATCTACAACTTTCCACGCTGAGATTTTCCGAGGAAGGCATTCTAAGCCATTCCCACCGTACTTTAATAAAATAAAAACAAGAAGATAGTAAAGCTAAGCTACAACCTTCCGCCAAAAAAAAAAAA SEQ ID NO:33 LateLPS-045 GACCGCTGTAGGAACACTAGCAGATTCCGGAACATAGGTACTTTGAACATCTTTCACTCCTCACCATATGAATAGTGAGTCGATGGCGGCCTTAACAGTCGAGCATGCTTTGATTTCGTCTCTCTCTCTAGTGACCGGAAATCAATCTCATTATATATGTCATTATGCATTCATTCCCACTTCCTAACTTTCATTATTGTTCAAAACTCGCCTTCCTGAAAATGCTATAATAGTAGGGGAATATTGAAAAACTTCCGCCAAGCTAAAAAGGCACTTAAAGCACCTGGATTTGAACGAGGATTTCCCACCCCGATGAGGGGGGGTGTCTTTCCATTGAGACGATGCCTTACTCGGCAGACCCTGTGGGGGTCTTTATAGGTGACTTAATACTTAAGTATAGGACTAAGAGAGAGGAAGCGACCGCCTCTCTGATCAAGCCTTTACGTGCGACGTGCCCAGGTAAAGGCTGATCTCACCAAATAATTCAGAGAAAGAAGATGACTCCACAGTAGCGAAACTCCTACATTGTCTTACATATCGTAACAAGCGGTC SEQ ID NO:34 MiddleLPS-046 GACCGCTTGTGCGTGGTGTCCAAACTAGGACGCCTTAGTTTTCCTAAGAAGGAAACCCAGGCGTTGACTTGAGGCAGACTTGTGCTTCTGGGTACTCTCATCACTGCGTGACCTTGAGAAAGGGACTTTACCTCCAGGATCCTCAAACTTCTTCTCTGTAAAATGAGCATTGTAATAATTATATCCCAGGCTTATGTTGGGAATATTCAATAAATGCTCCCTTCATTCTTTAAAAAATAAGTAAAGACAGCCTGAATGGGAGCCACGTTCTCATTCTTCTTTCTCTTTAAAAAATAAGTAAAGACAGCCTGAATGGGAGCCACGTTCTCATCTTCTTTCTCTATGCAAAATGTATTGTGTAATGTTTGTGTACTAGTAGTTCAAGAGCAAATAAGTAGTTGGTTAATGGCTAACATATTTCTTAAATTTGTAACTGTTAAGATAAACATTGAACAAGGAAAAAGATTCGTAACTGAAATGTAAAGTCATTTGACCCTGGATAGTCAATGACAATCTTATTCACAGTGTAATAAGTAATTCATAACGAGATGATTATTATGAAATTATCAATAGCCTGCTATATCACTTTATGTTTATGATCCACAAGCGGTC SEQ ID NO:35 All LPS-047GACCGCTTGTGGAAGAAAAGAAAGAATCTCTTTCGGATTCAATAGGCGGTATGGGAGAGTCTGCTACTGCCTCTTGGATTCCAGGAATCCTAGAGCTGGGAGTATGAGTTGGAGATGATGAAGGTGTCTCTTACCTATTTCTTGAAGTGGATGGAGTTGTGAAAATCGACTTCTAGCTTCAGCTAAAAACCTTCCCCTAGAATCTCTTGCTCTATGCATATCATTTTTATTTTTTCTTTCAAGATAGGGTAATAATTCTCTTTCTGATCTTCCAGGTCACTCTAGGTGCAAGAAGAGAGCATAGTCAAGGAACTATTAAACCAATAACTTTCTCTTTTCTGATCCTCCAGTTCACTCTAGGTACAAGCGGTC SEQ ID NO:36 All LPS-050GACCGCTTGTGCAAAGTAGATACCGTCCTGTTCCGGTGAATTGAAGTACATTTTCAAAATGCGCTACTATGACATTTTATAGGATGTCTGAGTGTAAAATAATGGTACTGGTTGTTGCAAAGAATCTGATGTTTGGATGTATGGAACTATAAATAGATGTTATTTTCTGATCCAGAAGGCTTTCCTTACCAACTGATTTCATCTCAGAAACTAAAAGCTCTTGAACTTGTGTAGATGGGGCTTGGTCATTGTAGTTTAAATGCATTATGTAGTGGCAAAAAAAAAAAGTTATAGCCTACGTTCAAATGGATTTGCTCGACAATCAAATGAATTACAATTGAATATTCATGTATACCCAAATTTTAAATGTAGAATGACATCATCAATGTAGACAAACACCACTGTGCTTGTCCTTGATATCCTCTTTCACCATATAATTGGTGGCTACTCAAAGTCACTATCTGATGCAACTACAAGCGGTC SEQ ID NO:37 Late LPS-051GACCGCTTGTTCAATGCAGAATCTCGAAGAGATGTCTTGGACAAATACTGAACTGGCACGATTGGTGTAGTGCGGTTCAAAAGGCGCTCCAGATTCGTCTGGAACGAATCTTCATACGCTGAACAATTAGACATCTTGTACGCAAGAGAATTACGATCGGCCATATAAAAACCCCAAAGAGAAGAAAGTGTTTCGAAATTCTCCCAGAAAACAGTCTTATGCCACCGATTTGTCTTTTCAACATGCATTTGCAATGAAGTCTTTGGATTCTTACTGTGAGTGCTGATCAGCAACGGATTTTCGATCTGTATAGCTCTGCCGATTCCTGGTTAAAGCAGCTAAGAGTTAGGCATCCAGATTTGAGTTTTTTGCATCTCACAATGTTTGAATACATTCAAATCCATTGTTGGAGTAACCTAACAACAACTGTACTCTTCTTCCTATTTCTGAAGCCCTCTGCCAGTTTAAGGCAGAGAACTGAGTATCTACAAGCGGTC SEQ ID NO:38 Late LPS-052GACCGCTTGTATAATAAAGTGGTACCGCGTCCTGCAAACAGGGTTCTCTTGCCATCCTGCTACAACCCTGCAGTGGTCGCAGTAGAGAGAATCGGAGCAACGAACGTTTTCCCGAATATATGGAGCGGGAGGAAGAGTTTTCTTGCTGATGATCCAATCGGAGTCGAACTGCCACCGCTGGATGAAGGGCGGCGAGGAAATCTTGGGGGGCAGAGGCCCGTCGGCGTAGGAAATAAGAAACGATTTGATATGGAACGAAAGGGCCCGTCCAGGGTTCGATCCCCGGCAGGGCAGCCAGCCCCGAACTAAACAAAACAATAAGAACAAACAGCAAAGTAAAAGAAAGCACCAGAAGAAACAGCAGCAGACGAAGAGTAAGGAGCTGC CCACAAGCGGTC SEQID NO:39 All LPS-053GACCGCTTGTAATCCACAGCATTTTCAATAACTTCCTGAGGTGACATCCACCTCCACTCAGAAAACTCGGCTGCATCTGTCCCATCACCAGCTAGATTGATCTCACTCTCGTCTCCTCTAAATTTTAGGAGGAACCATTTCTGTGCTTGACCTTTCCATTCGCCTCCCCA CAAGCGGTC SEQID NO:40 Middle LPS-054GACCGCTTGTATATAATGTGAAGACACAATAAAATTTTGTCCAACAAAGCAACCAAACGACCAAAAATTTAGCTGTGACATCAAAAAGCTCAACCCCTACAATGAATGTAACCTTAATCTAGAAAATTGATCCATGATCTCCACTGAATTTCTCGTCATCCTGAAGAATGAGAAACTTAAATGTACCCGATTCCCTCAACCAAGCCCCCACAAGCGGTC SEQ ID NO:41 EarlyLPS-055 GACCGCTTGTAATCCACAGCATTTTCAATAACTTCCTGAGGTGACATCCACCTCCACTCAGAAAACTCGGCTGCATCTGTCCCATCACCAGCTAGATTGATCTCACTCTCGTCTCCTCTAAATTTTAGGAGGAACCTGTGATTGGTAGGGGCTTGTCATAAATGATCAAGACGACCCGCATCGTGATGCCAAGCTTAGTCTTTCTACTTACTGTCTATGTAATGGTCACGGGCCCTTCTTATGTTTATGTCTCTTTGAAATGGACGATTTTTTTGTTTTAGGTATACGGGCCCTTCTTATGTTTATGTCTCTTTGAAATGGACGATTTTTTTGTTAGGTATTCAGTTTCTGAAGCTGTTTTGGTAGTAAACTGGGCTCAATCATTTCTGTTGCTGAACTTTCCATTCGCCTCCCCCACAAGCGTCAGCCGAATTCTGCAGATATCCATCACCTGGGGGGGCCGCTCGAACATGCATCTAGAAGGCCAATCCCCTATATGAATTCTATTAAATCCCTGGCCTCGTTTTA SEQ ID NO:42 Early LPS-056GGTGCGATCCAGAACTATCATCTCTCACTGCTCGTGAACAAAATGCTGGTCATAGCCATCACTAAGGCTAAGGTACTATCCAGCCAAACTGATCTCAAATAATAATTTCATAAGCTTAAATAAATAGTCCAGCCAGTAGATGGAGCCAAAAAGCCATAGAAGCTCAAATACTTGTGGTATCAATCTCTCCTCTGTTAAGGGAGGTATCAGATCAGAAGCACTAATCAAATGCATACATAAATGCAGTAGACTGCAATAAAACAAAATCTGCAGATAGCAACAGAGCGCTTAACGAACGGAAAAGAGTTTAACTTGATCTATCACAGGATCGCACC SEQ ID NO:43All LPS-057 GGTGCGATCCACAATAGTTCGTACGAGCGACGTCTATCTGGTTAATCAGAACACATATCTAATTTGGAAATTTGTGGGCATAAAGCTCCACAGTGTAGGTGGGCTAATCCCATGAAACATTACTCTTCAAACATCATACAACTGAGGTGGAAATGCAAAAGATTATTACTGGATGCTGATCTGGGACTAAGGTGGTGGCCATTGGTAATGTGTGTTTCAGAAATATATCTTCATGATGATCAGTAGTTGCATCTGGTTGGAAGAATGATAAATTCTGGTAATTTGTCTTGGGATCGCACC SEQ ID NO:44 Late LPS-058GGTGCGATCCAACTAGAAGAATATAAAGAAAAATTACGGACTACCAGAAAACATCACATCACAGTGTATGCATTCTCAATAATCAGAACTGTACTGGCTAATATCGCTGTGCCTGTCGTTTCATTTTCCTGTCATCCGCATAGGGCCCCTCATTTTCCCTATCTTGCAGAAATCCAAGAAATGCAAGAAAACCAAAAAGGAAGAAACCCCCAGAGGAAGAGTCCGAAGAGGATATGGGTGTCAGTCTTTTTGACTAGATTGGAGGATCGCACC SEQ ID NO:45 EarlyLPS-059 GGTGCGATCCCAGAACATTTCAGACAGATTAAAACAAGATCTAGTCAATCCTACAAGGGAAACTTTTGTCAAGATCCGGATCCAGATTTTCCTCAAGTAAAACTAATCTCATTAAATCCAAGCCAATCTCTAGCAAAATTCAAACACTTTTTATTAAATCCAAGCCATATATCTGGCAAATTCACCGAAATATGTACAATCGCAGCGCATTGCTTGGCTTGCGACAGAAACCATATTCGCACGTCTTCATAAGGCTTTGGATCGCACC SEQ ID NO:46 All LPS-060GGTGCGATCCAACAACACAGCTTCACACTTACTCCATCCTCTGGAACTCTCATCAGATTGTGTTCTTCGTAGACCAAGTTCCTGTGAGAGTCCACAGGCACACTGAGGCTACAAGCGATGTGTTCCCTAAAGAACAGGGGATGTACATGTTTTCCAGCATTTGGAATGCAGACGACTGGGCAACCAGGGGTGGGCTTGGGAAGACAAACTGGACTGCCGCTCCATTCAGCGGATCGCACC SEQ ID NO:47 All LPS-061GGTGCGATCCCAACACCAAGTGAGAATGAAGCAATATAAATCAGCAGACTCACTAAAGCCAAAACAGTGAAAAATGTTTCATATTGGGAATCTGCTCCAGAATGAGCCTTCAAGTAAAATGACAAACTAACGAGGAAGAGACATACGGCCATGCCCCCAGATGAGACCATGAGGAGGAGACGTCGTCCGGCTTTATCCATGAGCCATACAGCAACTGCAGTCATGATGACCTGGATCGCACC SEQ ID NO:48 Late LPS-062GGTGCGATCCAGGAAATCATCAAAGGGGAGCACATCCAATGTGCAAAATAAGATCATCATGCAGCAAGATCTCTGAAATATAAGCTCTGTAAGACCAATCTGAAGTGCTGATGATCAATATGAACTGAAACATCATGCCACAATGGGCTGGTACTTGTGCAAAATTCTCTGGCATGTGATGAGAATCACATGGTTACCTCTTTGGATCGCACC SEQ ID NO:49 Early LPS-063GGTGCGATCCAAAGAGCCTTCTTGCAGACAATCCGTGAAAACATGGCTATACAATAAATTCCCAGTTTGGAATTCTAAATAAAACTGTTCAATATTTGAAGGCCTCTGATATCACAGAGACTGATATTAGAATGGAAGCATGTAGCAACCCTAGAAGCTTTCGCATAAAGATACCAGATTAATTCATAAGAAGGATCTCTCGTTCACCAGTCACATATCACAGTCGG ATCGCACC SEQID NO:50 Late LPS-064GGTGCGATCCGTTAGATGAGCTGCCAAGTATGGAATTATTGACATTTTTGGACGGGTTATGGGCAGAGGGATGTGCCAAGCTGAAGAAGATACCGGGGTTGGAGCAAGCCACAAAACTTCGAGAGTTAGATGTTAGTGGGTGCCCTCAGTTAGATGAGCTGCCAAGTATGGAATTATTGACATCTTTGGACGGCTTGTGGGCAAAGGGATCGCACC SEQ ID NO:51 MiddleLPS-065 GGTGCGATCCACATAGTTTGAATGCAAGGAAATTGCACATACTTCGTGGGGAATTTCGATGGCAAATCAGTCCAGGTAAATGACTTCTCAACATAGGTCCAAAACTCTTTCATAGACCAGATCTTGACCGTGTTGTCCATGCCACAGCTGCAATACGATATACATCTGAAGGATGAAAATCTACACTGAGAACTTCATTGCGATGTCCCCCAGCTCCAGCAAATATCAAAATGCATATTCCAGTTTGAACATTCCAGAGTCGTACAGATTCATCTTTGCTAGCAGATAAAATAAGGGAAGGTTTCAGTTGCTTGGGTCCTTATTTCATTCACAGAACTCCATGGCCAACGAAACTCTTATGGACTTTTCATTTGCACATCCATTCTCGAATTATACATTGTGACCGCAGCCACTAATAATGGGGAACATCACTCGCCTGCCGACTTATGTG TTAAAGAATC SEQID NO:52 Late LPS-066GGTGCGATCCCCTCCATTTACCATGGTATACTGTTCCAAAGGTTCCAGAGCCTAGCTCTTTCAATTCTTCAAGGTCAGCATTCTTTATATCTGGAAACTTCGCTAGCTGTGTCTATAATCACGAAACCCAGACGGGGAACTAATAGGCGATGAAGTTCTCTTATCCATAACCGTTGCAAAGATCTTACACGGAGTTTTCTCTTCTTCTGCGTGGCTTTtCTTTCCCGTATTCTCGGATCGCACC SEQ ID NO:53 Late LPS-067GGTGCGATCCATACATGCGAGGGCGCATGAGAGACTACCACAAATCCTACATACCTCCATTCACCCCTGGATCGGTTATACAAGGATTTGGGGTGGCTAAAGTGATACTCTCAAATCACCCAGACTTCAGAGAGGGTGACTTGTATCTGGTACTATAGGATGGGAAGAGTACAGCATAATACCAAAAGGGAGTAACTTAAGAAAGATCAAATATACGGACGTACCACTTTCATATTTTGTGGGTGTTTTAAGAATGCCCGGGTTTACTGCTTATGCTGGATTCTTTGAAGTTTGCTCTCCTAAAAAGGGGGAGCATGTTTTTGTCTCTGCCGCTTCAGGAGCTGTTGGCCAGCTTGTTGGGCACTTTGCAAAGTTGATGGGTTGCTATGTTTGTTAGGGAGCGCGGGTAACAAACAGAAGGCTGATCTGCTGAAACATAAAATGGGCTTTGATGATGATCTCCACCATAACGAGGAGCATGACTTCGATGTGGCTTTAAAAAGGCATTTTCCAGATGGGATTGCACC SEQ ID NO:54 Late LPS-069GGTGCGATCGAACTGAATGAATGAOGTTGCCAAGCTATGTTTGGGAATTAAAACTTGAATGCCGTTATTCTCTCCTTTTTCCAAAAGGGCCTTTTCTGCCAGAAAACCTTAAATTTCTGACTGGTTTCCAAGTCCAATTTTTCCAAATATGGATTGGTTTACCATTGAAGGCACCACCATGCTCTGAAAGTTATGGACTGCACTTGCCCCAGTGCTATATTTAGTCCAGATAGCGCTTGTGTCTCTAAATGCATCTCCCTGCTCGGATATCACC SEQ ID NO:55 LateLPS-070 GGTGCGATCCGAACAGAGGGAGCAGATTTTGCCCTGCAAGTATTCACAACATTAGAGAAGCCCTGCCAGAGATATGGGAGGAAGAAGATGCAGAGAACACCAAAAATGTTGTGGGATCAAGAGGAGCGGATGCAACTATAGAAACTGTTGTCACGGCATAAGCCATCGCCTCATTGAATGAGGGAATGGAGGACTAGACAAATCCCTTTGGATCGCACC SEQ ID NO:56Middle LPS-071 GGTGCGATCCGATTGGGCAGCTGCAGCCTTGGGAAGCTTTAGAATCAAATTGCACTCATCCTCCAGGAGGTATTGAGAAGTCAATTTCTCAAGGTCTACAGTGACAGAAGGAACCATCTTGACAATCTTATCAGGTTTCCTGCTCTGGTTAAACACTTCAACTTTGACAGGACGAGAGTATGTGACTAATTCATCTTCTTCATCAGACTCTACATCTCCTGTTTCAAGAAACAAAGATACTGATCATCACTAGGGCAAGAATTGATGATTTTGATATCTCTGGAGAAGCCAGTGTTTACATTGGTTTGCTTCATGGCCACCAGTCTATGGCATAAAGCTTTCCCGAAAGGGTACTTGGCAGATTTAACAGAGCCCAACGTTATATTTAAGGCCCATCTCTTTGCTCTCAAAATTTTTCTTGCATCCTCTGGAGAATATAAAACCCCTTGGTGTCTCTTTCCACAAACACCTTCTCATTGATC SEQ ID NO:57 Late LPS-072GGTGCGATCCAACTGAGAGGGTGTTGGTGGAAAGATGACACCAAGTGGGTTCTATATTCTCCAGAGGATGCAAGAAAAATTTTGAGAGAAAGAAGATGGGCCCTTAAATTAACGTGGGGTTCTGTTAAATCTGCCAAGTACCCTTCAGGAAAGTTTATGCCATAGACTTGGTGGCCATGAAGCAAACCAATGTAAACACTGGTTCTCCAGAGATATCAAAATCATCAATTCTTGCCCTAGTGATGATCAGGAAGATGTAGAGTCTGATGAAGAAGATGAATTAGTCACATTCTCTCGTCCTGTCAAAGTTGAAGTGCTTAACCAGAGCAGGAAACCTGATAAGATTGTCAAGATGGTtCCTTCTGTCACTGTAGACCTTGAGAAATGACTTCTCAATACCTCCTGGAGGATGAGTGCAATTTGATTCTAAAGCTTCCCAAGGCTGCAGCTGCCCAATCGGATCGCACC SEQ ID NO:58 Late LPS-073GGTGCGATCCATGTAGTGCCAACTTACGAGATCACTAACTTTAAAACTATCATGCAATTGGCCAATAGAAGCGACACTTGCTGTGCCAAAGTATCGATAGGCTACTCCCGATGGCTCAATCATATATAGTTGGGGCCCATCTCTATCATAACCTCCAAGGATAACTCCAGATCCAAAAGGCCTTAACCACCAATATAGTGTGCACAAATGCACATAACTGGCAACACGTTCACAAAGTTCCTTAAT SEQ ID NO:59 All LPS-074GGTGCGATCCCATGGGATAGTTGCAAGACACACAAATTTGTTGTGAAAGAAGAGAGACACGCACAGACAACCATATGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGCAAAATTCAAACACTTTTTATTAAATCCAAGCCATATATCTGGCAAATTCACCGAAATATGTACAATCGCAGCGCATTGCTTGGCTTGCGACAGAAACCATATTCGCACGTCTTCATAAGGCTTTGGATCGCACC SEQ ID NO:60 Early LPS-075GGTGCGATCCCACTGTAGTGTCCTTGTTGAGCATAGTTCAAGCTGTTCTGATTCCACCAGTTAGTGGCCCAACACTGCGAGGTGCTGCCATTTCCATTCCATTCACAGACGTCAGTGTTGAAATTCATATAGGAAGCCACAAAGGGTGAGGAAGACCAATCTATTTTACTCGCCCCCCTTGAGTTGCCCACTGGTCTCCGCTCCATATGCTAGAGAATACTCTCATTGCCTGCTCATTCGGATAGGGAACGCCTATGTTTTCATTGTTTGCAAATACTCTGATTGGCAAACCATCAACGAAAATCGCAATTTGCTGGGGGTTCCAGAGAATAGAGTAATTGTGGAAATCTGCTGTAGGATCGCACC SEQ ID NO:61 Early LPS-076GGTGCGATCCCACACTCCTAACCCTATTATATGTCTCCCGTCCATGGAGTCATAGAAGGAGTACGATAATATGCCCTTCAGCCAAGCGAAGTATGACTTTAGTATGGCCAGGCAGCAGTATGAAAGCACATCTTGTTTCTTCCAGGTCGGCATGTATAGTCTCCGGAGGCTAACAATGTCACCCAAAGCTAATTGCGCAAACGGAACTCCTCTGCTGATCTCCCGGGAACTTAGGCGGAACCACCCTGAATCCACTATTCTCACCGCGCATTTCATCCCTTTGGTGAACGCCGCTGCCTCTGGTAGATACAGAGCTGGCTTGTCTCCACTGGAACCCCCTTTCCGGATCGCACC SEQ ID NO:62 All LPS-077GGTGCGATCCAAACTGTGGTTATCGGTGGAGAGATTAAGCAATTTATTGGAGTAGCAAGTACGCTGAATTAAGGGGGTCCATCTTCAAGCAAAGGTTCCTTTGGATGACTATGTGTTCTGGAAGTGTTTATGGATCAATCATCTCATAAATTTTGGTAATATATAACAGAAGATTATGGCATCCAGTTAGGATGGTAGTTTCATTGAGGTATAGTAAAAACTACACTAGTCTTGTGTTGCCACCCACTTTTCAGAGAAGTCAGGAGGTCTCTTTGTGAATCATTGATAACTTTATGAGTGGGTACCTAAATGAAATATTTGCATCTTGAGTATATACTCAATTGATCTTACTTGTGGATCGCAC SEQ ID NO:63 Middle LPS-078CTTGGTACCGAGCTCGGATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTTACGGCTGCGAGAAGACGACAGAACACCTATCATAACTTGAATTCTGATGCAAATCGGAATTTGCCAAAAACTTGGACGGAAATATAATAGGCAATATCATCCCCGCAAGTAACAAAAAAATTGCATGAAAGCTCAAATCCTATGTGCTTTACACCTTGACTGCATACTTTCTCATTGGAAAATACATCTCTTTCTTTTTCTGTCTCTCAGTCTTCAATGACGGCTGATGCTGGTAAGGCGTCGCCTGATAGCACGAGTCTTCTTGGGACGCAAATCAAGAGGCAGGTACTTCTTTTTTTTGTATGCTTCTCTTAATGCGGATCGCACC SEQ ID NO:64 Late LPS-079GGTGCGATCCAAGATTGTACGGCACAGGCAAATGCTGTTCTTTTTCTTAATCACGATGTGCTTGAAGAATATGAGCGCCGATGTGAACAGATCCACAACCTGGAGTTAAAATTGGAGGAAGACAGAGCAGTGCTGAATAGGAGCTTGGCAGAAATAAATAGTCTTTAAGGAATCCTGGCTTCCCACATTGAGGAGTTTGGTTACCAGAATTAATGAAACTTTCAGCCACAACTTTCAAGGGATGGCTGTTGCTGGAGAAGTTACACTAGATGAACATGGCATGGATTTTGACAAGTTATGGTATTCTAATAAAAGTCAAGTCAGGCAAACTGGACAGTTGCAGGTATTGAATTGCTCATCATCAGTCTGGAGGGATCGCACC SEQ ID NO:65 All LPS-080GGTGCGATCCGAGGGAAGCGATGTAGTCTTGCCCCAAGCGACGACCATGATCCCTTATTCTTGGGCAATATGTGCAAGACGTGGACAAATGAAGCGGTTAAAGGGAAGCTTATGGACTATGGAATAGAGGGTCTTGAAGAGCTAACTCTAGTGGGTGATACTCAAAATGAAGGAATAAGCCGTGGTTTTGCATTTATAGCATTTCTACGCACATGGATGCGATGAATGCATACAAACGCCTTCAGAGGCCAGATGTTATTTTTGGTGCTGATCGAACTGCGAATGTGGCATTTGCAGAGCCACTGCGTGAGCCTGACGAAGAGATCATGGCCCAGGTTAAGTCAGTGTTGTTGATGGGATCGCACC SEQ ID NO:66 Late LPS-081GGTGCGATCCAGTCCTGAAAATGTACTTTACCATTTGTATAATGATGTAAAAATCTTGGCCATAGTCTGGTCAAACCAGACTGTATTGTTGCTAAAGTATGGAAATTCTGGCCATATTTTTGTCTAACCAGACTGTATTGTTGCCAAAGTTATGGGAATTCCGGCTATATTTTTGTCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGATCATAGGGTTGTCTGTGCGTGTCTCTCTTCTTACACAACAAATTTGTGTGTTTTGCAACTATCCC ATGGGATCGCACCSEQ ID NO:67 Early LPS-083GGTGCGATCCGCTGGAAGGTGGGCAGCTGGACATCTGGGAATTATAAGTCGAATGTCAATTGCTGGGCCATCTGGGGGATGAGCAATAGCATCGGAGGCCAAGTTCTTCTGCAGCCGGGCACCAAATGCCATGTGGAGGTCTGAATCTTAGTTTGGAGGTCGAAGTTTCAATCCCCTTGTGTTTACTCTGTTTCTGGTTTTATTTGAATAATTTGAGCAATTTAATGTGGGTCCTTAGTGCTTCTGTGGATCAGATTCTAGGGAACGCCATCCTGATAAGTAAAGATCCGAGTTTTAATGGAGATTCAATCTATCAGAATTCCATGGTGGTTTAAATTCCCTTGTACTGTTGATCTACGTCGCTTTGTATATCAGTGTGTGTTAAGATTTTCTCAGAATCCACAGCTTTGTTATGGATCGCACC SEQ ID NO:68 Middle LPS-084GGTGCGATCCAAGCACTTACGACTCCCAACAAGGACGGGAAACTCTAAAATCGGAAATATCATATACTGAGGCATCAACTTTGTTGATAAAACTTTAAACAAGAACAATATTTGCAGCATATTAGCCCACATGCCATAATGACAAACAAATATGAGAACACTGCCTACAGGTTTGCCAAAAGCATGGCCCTCACTTTTGCCCTGAGGTCATCAGGAGCTTCTGAGGCTCGAGAAGGAGAAAAAGATTGTGTCACTTCAGGAGCTGAGGCCTCCACATCTTT SEQ ID NO:69Early LPS-086 GGTGCGATCCAAGGTACGAGCGAACAAGTTTCTTCAGCAAGCCACCTGGAACTTTCCATGAGTCCAAAACAAGTTGAAGAAGGCTTCTTTGGCTACTTTTAAGATGCTGAAGTGATTGTGCTCGCCTCTTGCACAGTTCAACCGCAATAACATTGGGTTTTACAAAACCGATTACCTGTTTAACCTGCTGTGCACTCTTTTTCGAAACATGACAAGTTCCAACAAGATAAACTTCGGCCCCATTCTCGCCATTCCGCAAATAAACCACGCTCTCATCTTCTGTTATCGAACTCGAGTGCATGCCACGACGCTCAATTGCAGGATTCCAACCCCGGACTTGCGAATGGTGCAAAGCGATGCCCGTTTCGTCTCAGCGATACTGCTAAAGATCGGCAGACCCGAACCAGTTTGATGCTTCCATTGCCTTAAACATCCAGAGTTTTCCTTCGACCTTAAACCCTAACAAGATTACTGATTTCTGGTCCGGATGTTCACTGTCTGTTATACTTCTCACAAATCTGTCACACTCCTGATAATCTTCGGTATTGAACTTCATTGAATTGAATTTTCCTTCTCATTGGAATTCAATTGTACCTTGTAAATGTCTGGATCCTACACTATACCAATATTTACAGGTCTGAGTATTTTGCCTGTAGTATAATTATCTTTCCTTCGGTCTCGTGTTTCCGTATTATTCGTGTAGGATCGCACC SEQ ID NO:70 Late LPS-087GGTGCGATCCCGGGGGGAGGTTGATGTTCTGAGAGAATCAATGAAGGGATTTCAGCTGAGCTTGCCTTTTTGAAGACGGAATGCGAACAACCAGTCATTTGCAATAGCGAGAATTCTCTTAAGCCACTGCCTGCTGGGGAGGCGAGTTCTGATTCCGGTGATTGCATCACTCAACGGCAGCAGCAGCGGCAGAACCTTTAGTTTCCCATGACAGGTCTCTCTGTACAAGTATCTTCCTGTATGATCTAATCCGGGTTGTTCGATTATCGTGATGTCTCCTGTATTGACATATTAGCAGAATATTACCATGATACGATGTTAAGTGGCATGGTTTATGCCCTGCATGTTATGTTATGGAGGAGGTGAGGCATGTGGCGCTCATGGGAGGGCCCACATGGTCCATGGACGTCTTATTAAACGCATAGTCGTGAATGAAAATAGTTCAATACATTCAAAATTCCAACACAATTTCATTACAATGGAAGTGACTTCGACTTGAATGTTCATTGAAGCATTTGCATGCACAAACAAAGTATACTAGATTAGAAGAAAATTGCAAAAAAGGACATTGTGCCCTTCTTAGTGAATATATAAAGATGTTCTTCATGCTGGATCGCA CC SEQ IDNO:71 Middle LPS-088GGTGCGATCCCAATAGCCAATATTGCCTCCAAGATAGCCTAGACTGCCTTTGCATAGTTCTAGAAGCCAGTCACCCAACCTCCCAAAAGAAATTGCGCAATCTTTCCCATCAGTTTCCCGGGTATGTGTTCTGTCATTCCCCGAATTTTCTTTGGTTTCACTAATAGATTTCTTTCCATGCACATTGCTTGTCTCCAGATCTTTTAGGTGTTCATCCATCTCTTAGTAGTACTAGATCGATGGCTTCCAAGAGAACAGGATCATATGACACTGTTGGAAATGTAGCTGGAGCAGCAGTTGAGCAAGTGTCCTCTAGTCTATCTATCTATGAAAGATACACATTGTTTCTAGACATGGATATCAAATTGAAATTGCCAGAAGTCCATGAAACATTTGCCGCCTTTTGAAGAAAGGCTCCAAACTGTCAGGGTTCGTGAACATCACATGTTCTCGCTGTCTGATCCCCCC SEQ ID NO:72 Middle LPS-089GGTGCGATCCTCAGGGTAATGGCCTGGCTGAATCAAGTAACAAGAATCTTATAACCATTATCTAAGAAGATAGTAGGAGATAACAAGCGGTCTTGGGACAACAAAATCAAGTGCGCTTTGTGGGCAGATAGGATAACTAAAAAGAAAGCCACTGGTAAAAGTCCCTTTGAACTTGTCTATGGCATGGATTTGACATTACATGCCCATCTTAAATTACTAGCTTACCAACTCCTTCAACATTTTTCTAGTGATAAAGGTGTTGTCCAAAACATGGTTGATCAAATTGTGCAGTTGGATGAAATCCGCAGGAAAGATTTTGATAGTGCAAAAATCAGTCTCCATTAAGAAAATCTTTGACAAATCTTCTCGGTCTAGATATTTACAGGTTGGAGATATGGTTTTACTATGGATTCCACC SEQ ID NO:73 Late LPS-090GGTGCGATCCTGCAGGCTTAGATAGTTTCGGCGCTCCTCTGAAAGAAGCACGAGTAGGTGTCTCCACATTAGGTGGCCTGATCCCTTGCCTGCACTTGCAGCTTGTCTTACAACATCTCCTATGCTTTGATCCAGGCTTTTCACTGACATAACTTCAGGGGCTTCCTTCTCCCAGGGCCGTGCTGCCATCCAGCGTTCTAGCCAGCTCCATCCCCAATTTGGCTTGTTTGGGTCAATTTCCATCAGCATAGGATGAGCTGCTCCTCGTGTGCTTTTCAATGACTGATGAGAATATGCGTTATGCCAATGCCCTTTCTCGCTTCATGGCTGCTTCTTGCTTGCTTTGCAAACTAGCCTCAATTTCCTCTTTGGATTGCAACTGTCATCCAATCCTTTGCTTCCATACTGGATCCAC SEQ ID NO:74 Late LPS-091GGTGCGATCCCAAATGAACATTCAACATTCGATCATGTCAAGCGCTAAATGCCTTGGCAGCTTAAAAGCTAGACTCCGCAAGTGACCCTTCTGACTTAGTACACATATTAAGCTCATCAAGGGTCCAATTCCATGAAAAGAAATTTTAAAACGGTTACATATTCACAAGAACAGCACGAGATTTCCCAGATAGTCAACCACCAACTTGCCCTATCAGCCCAAATATTACTCATTCCATGTTAAAAATAGCAAATTTCCAGATAGAATGTCGAAAGAGATCTTCATGCACCATATATGGACTCTTAAAACCAGCCAAAATCTATACTGCCATGCTTGGAT CGCACC SEQ IDNO:75 Late LPS-092GGTGCGATCCTGGAGAGAGAAGCAAAAAGCCTACCATCTAAATCTACATCTAAATCAGATATCTTTACTGTGAAAGGAATTGAATGCTGCTTCAGATATCCTACAAGAATAAGAAGAAAAGAATGATCAACTCCAAATCAGGCAGATGGCTCAGAATTTCCCGCAGCTTCATTTTCGACGGCCTCCACAACACCAACCTCGGCAGGACGTATTACTCTGCCATGAAGTGTATAGCCAGGCTTCAAAACCACAGCCACACTGCCAGGCTGCTTACTAGCATCTTGAACTTGAGATACTGCCATGTTGCATATGAGGATCAAACTCTTCATTTATTGG ATCGCACC SEQID NO:76 Late LPS-093GGTGCGATCCCCAGAGGTTATTTGGGTTCAAAGTATTCTACACCAGTTGACATGTGGTCATTTGCTTGCATAATTTGAACTGGCTACAGGTGATATGTTATTTGATCCTCAGAGTGCAGAAGGTTATGACCGCGATGAGGACCACCTTGCCCTGATGATGGAGCTTCTTGGAAAAATACCTCGTAAGATCGCCTTAGGTGGGAGCTATTCACGGGAACTTTTTGACAGGCATGGGGATTTAAAGCACATTAGACGGCTTCGGTATTGGCCCTTGGAT CGCACC SEQ IDNO:77 Late LPS-094GGTGCGATCCTAAACTGTATGTCTCCACAATTGTCTTCAATATAGAAGCAGCTACGCCCCTCCTAAGTCATCATAAGTTAAAAACTTCATCTTTCCAATACAATTAAACTATCTAGCTTATCAGTTTGGAATAGAGATACAAAATTACAGATAGATTAGCGAAACTGTGCCACAAAACCTCTTCAAAATTAGAAGCATGATTGTCTACAACTCCACTTCAAAAAGGAGCTGAACCAGTCCTTCGAAGGGTGTGCTTTGGTTGTGGTGGAGGTACAGAAGGCAGCTAATTTCTCCAAGAACTGCTGTTTTTTTAGCCTCTCATTCTCCTCTTTAAGCTGCATCACTTCATTCTCTAGCTCATTTGTGTATGCCTGCTTTCTTGCCCTGGATCGCACC SEQ ID NO:78Middle LPS-095 GGTGCGATCCGAGTGATGGCACAAAGAAAAGCAATGATAGAAAACAAAGAACAGGTAGCTCAGAAGGTTCAGCAACTAGAGAGTCAACTTCGAGTTAAGGAGGGCGGGAGCAATTGGCAGATTCTTCCAAATTTGTCAAGATCTCTTGGCATGAGATGACCTTATAGGATGTTAAGGAGCAAGAGGATTCTAGGAATAATGCCAAGGATAATAAGACTAAAAGGATGCTTCAAGACCAGGTGGCAAGGAAGGCTTCTAATTCAAAGGGAGTTAGCAACGGCAACAGATGCAATTCTAGGATCGCACC SEQ ID NO:79 Middle LPS-096GGTGCGATCCTAGAATTGCATCTGTTGCCGTTGCTACTCCCTTTGAATTAGAAGCCTTCCTTGCCACCTGGTCTTGAAGCATCCTTTTAGTCTTATTATCCTTGGCATTATTCCTAGAATCCTCTTGCTCCTTAACATCCTATAAGGTCATCTCATGCCAAGAGATCTTGACAAATTTGGAAGAATCTGCCAATTGCTCCCGCCCTCCTTAACTCGAAGTTGACTCTCTAAGTTGCTGAACCTTCTGAGCTACCTGTTCTTTGTTTTCTATCATTGCTTTTCTTTGTGCCATCACTCGGATCGCACC SEQ ID NO:80 Middle LPZ-001ATCTAGATCATCGATCTTGTCCAAATTTTAACTAGTGAATAGTTTAAAAAAAAGCAACTAGCAGAAGAGAACCTAACCACTGACAAATTGCAAATACTCTAGAACACTATTCATCATTTTTTGCGATTCACGCTGGACCCACAAGAACCCCTTGAGCTGAACTTTCTTTTCGTTCTCCCTCCTTTTGGATCGCACCATCTAGACCATCGATCTTGTCCAAATTTTAACTAGTGAATAGTTTTAAAAAAAAGCAACTAGCAGAAGAGAACTAACCACTGACAAATTGCAAATACTCTAGAACACTATTCATCATTTTTTGCGATTCACGCTGGACCACAAGAACTCTTGAGCTGAATTTCTTTTCGTCTCCTCCTTTTGGATTGGACATCNAATCCTGCAGCCGGGGATTCATATTCTTAACGGCGCNCGCGNGGACTCCATNCCCCATATGATCTTTTCATCCTGGCGCNTTTAACTCTGAAGGGAAACCGGNTTNCCCTTATCCCTGGA NATCCCTTCC SEQID NO:81 Middle LPZ-002GTGGAGTGTAAAGGTCAACGTGCCATCCGGGTACAAACTATTGTAGAAAAAATGGCAAAGTTAGGTCTGAAAATATCCATTTGGCCTGCTCTAGTTGTACAGTACATGATTTTGCACTCGCACAACAATGGACTATAATTATTTTCCTGGCAAAAAAAAAAAA SEQ ID NO:82 LateLPZ-003 GGTGCGATCCAGGACATGAGGCCGAGTTTGCCATTGTGATATGATTGAGGAAGTCCAGTCCTAAAATAGGTTTATCTTGATGTTTGACAAGAGATATAGAGGGGCATGATGATTCATTGATCTGTTTGCAGATCTGTAACTGCAACCATTCTAATGACATAATAGCGCTATTGTTGGGTTCGTGTGATGACATAATAAATTGATTTAATTTAATAACATCTGTTAATGCAATGGCTGTAGCTGCATCATCACCGTATCCATCGAATGTTCCATTTTTCCAAATGTTTGTTTCCAAAACCAGAACACCAAAATGTCCCCTGCGTTTGTNTTGAAAAATATTGGGCCCNTACTATACTATAATNTTTNGGCATACTATACTATAATGTTTCTCCCATTCCCCCCAAATGANTCCTATACAATCCTGGCCGNCTTTACACTCCTGACNGGAAACCCGGCTTNCCACTAATCCCTGGNCNANCCCTTC SEQ ID NO:83 Late LPZ-004GGTGCGATCCGACTGTGATATGTGACTGGTGAACGAGAGATCCTTCTTATGAATTAATCTGGTATCTTTATGCGAAAGCTTTTAGGGTTGCTACATGCTCTCCTCTTTTGTATGAATTTCCATTCTAATATCAGTCTCTGTGAT SEQ ID NO:84 M,L LPZ-005GGGGAGTGTCAAGGGATAAGTGGTAAGCCAGGTTTCCAGTCAGAAGTGTAAAGGCGGCCAGTGATGTAATAGATTCATATAGGGGAATGGAGTCACCGGGGTGCGCCGTTTTAGAATAGTGGATCCCCGGCTGCAGGATTTGATGGTGCGATCCTGCCCCTGATAATTTGGTTGCAATGGAAAATGCAGTATTAGGTGCGAGATGTAAAGCCCGCCCGGAGCGGTGCATGAAGTACTGCAATATTTGTTGTAGTAAATGTGCTGGTTGTGTTCCCAGCGGTCACTATGGCAACAAGGACGAGTGCCCCTGCTACAGAGATATGAAGTCCGCAGCCGGCAAGCCCAAGTGTCCCTGATCTAGCACTCAGTCCAGTCGCTCACTTCTTTTATTCTTTTTTTTTATAAAAGTGACGAGGCCGTTTTTCTTGTACTTGGTGGCCATATGTAGAGCGGTGGCTACTTCTCCTGTGTTAGGAAATGTTGCAGTACTAATAATAAGAACTTCTTTGGCAAAAAAAAAAAA SEQ ID NO:85 M,L LPZ-006GGGTTTCCTTAAGAGTTAAAGGCGCATGATGTATAGAATCATATAGGGGATGGATTCCCCCCGGGGGGCCTTTCAGAATAGGGATTCCCGGCTGCAGGATTGATAGTGCGATCCAAGACACAGTGGAGTACCACAATGGGGATCTGGCCAGTGCTTTGTGGCTATTCACTGCAGCTGTATTAAAACAGGAAGCCGCAAATGGCCAGAAGGCCATTGAACTTGCTGAGAGCAGACTATCTAAGGATGGCTGGCCTGAATATTATGATGGGAAGCTTGGACGATATATGGAAAGCAGTCTCGAAAGTGGCAAACCTGGTCAGTTGCTGGATATCTTGTAGCCAAGATGATGCTTGAAGATCCATCCCATTTAGGTATGATAGCATTGGAAGAGGACAAAAAGATGAAGCCGTCCCTCACTCGATCAGCTTCTTGGATAATGTAAAATGGGGAAATCCTAAACTTTCAGGCCACTCTTGAATGTTTTGTCACTTCTGTATGACAAATGAGGCAATTCATAGTACATGTTGTGCAAAAAAAAAAAA SEQ ID NO:86 M,L LPZ-007GGTGCGATCCCAGAGAATATTAGTTCATGTGTTGCTCTCATTTTCTTCAATATGCAGGGCAACCATTTGAATGAAATTATTCCTTTCGAATTTCAAAAACTTAATAGGCTAACTTATCTATCTGGAGCCGATTTTCATTGACGAGTAACCTGTAAGCTGGCCAGCAAAAGCCAACAGATGTTCAGCTCGTTGGAACCAGTTGAAGATGTAATAGAGATGGTGAATAATCGCGGACGGCTCGGCCAATGGAATATTTGTTGCATCATCATCAAGGGGGTATGAATTCCAAAGAACTTGTTGATTGAAATTCCCAAGCAAAATTCTGTGAAATGAAAAATTTATTGAGACCATTGGGCAAAAAAAAAAAAA SEQ ID NO:87 Late LPZ-008GGTGCGATCCAAAGAACACAAGATGGAGTTACCACAATGGAGGATCTTGGCCAGTGCTTTTGTGGCTATTCACTGCAGCCTGTATTAAAACAGGAAGGCCGCAAATGGCCAGAAGGGCCATTGAACTTGCTGAGAGCAGACTATCTAAGGATGGCTGGCCTGAATATTATGATGGGAAGCTTGGACGATATATTGGAAAGCAGTCTCGAAAGTGGCAAACCTG GTCAGTTGCTGGATSEQ ID NO:88 Late LPZ-009GGTGCGATCTGTGTGGCTCTGAAACATCCCGGCTCCCCTCTGCACTATAATAATCCCAAAATTAAGTGAACCCAACAGAATTTGCTCATATCTCTACAGTTATTGCAGACTGAGCAAAACCCTCAAACTCATGTGACCTCTCAATAGGAGCCCACGCCCAAGATTTGTCCAGCATGTAACACACCTGATCGCCGCCACTGCAAGCACAACCGCTCACAAATATCTTGTCACACCACACTGTTGCGCAAGTTAACAATATTCATGTCTCCAGGAAAGAAATGCCACACTTCCCAACATTCTCTTTACTATTATAGAACTTCCTTGTTGCTATGGAAAAAATACATTCCCAACGCAGAACCCCAACGGGGGTTCCCAATANCCCATTTCCCCCCTNTCCAANCCNNTNTGAATGCNCCCCATNCCCTATTGNATNNTTTAAATCCNGGCGCNTTANCTGGAAGGNAACCCGNTTCCCN SEQ ID NO:89 M,L LPZ-010GTTTCCCAGTCAGGACGTGTAAAACGACGGCCAGGGATTGTAATACGATTCACTATAGGCGAATTGGAGGTCGATCCGTATAGGTAGTTGGATGATGAACGGGCAAAGAAGGCAAAGGAGTACAGTGATGGATCCTGTAATTCCTGTTTCAGAAAACAGAAAATCTGCAATATAAGGATGGCTAAGCTTTTCAGCTATGAAAATATATGGTGCAGTGGCACTCATATCAGTTGCAGAGTTGTCAATATAACTTTTGTGAATAGGAAAGTTGTCCTCTTTTAGAGTGCAGAAATCCTGCAATATAAGGATGGCTAAGTTTTTCAGCTATATGAAAATATATGGTGCAGTGGCAAAAAAAAAAAA SEQ ID NO:90 All LPZ-011GGTGCGATCCTACAGAGAGCAGCTTGACGAGGGCCAAAAGGTTAAGGATGAAGAATGACCTCAGCTAGTAAGGTTTACAGAAGCAGCAGAGGCATCTTAACTGTTTTTATGTTTTGGCAAAAGTTGTTGCGTCGGTTGTTTAATCCAGGATTTCAGATGTATTTTGTAG A SEQ ID NO:91Late LPZ-012 ATTGTAATACGACTCACTATAGGGCGAATTGGAGGGTCCGATCCTGCGAGACCGAGGGTTCATTTTCCTTTAGACAACGACGTTCAGTGGCGACCAGAGTTTCCCAATCACTTCAGCGATTCTATTCCTTCGTTGTAATAAAGCTTAAGGAATCCATGCAAATTCCTGGAAGGTTTGAATATTTATATTTATTGGCAAAAAAAAAAAA SEQ ID NO:92 Late LPZ-013AGGTGACCGTCAAAATGATTGCAGAGGACTTAGAGAGGGAAAACCGTTCCGATCTGGTGAAGCAATTGGATGAAGCAGCTCTGGAATTGATTCCCGTTCTGATGATATCGTACGGCTAAGCTCAGCTCTTCAGGCAATTGGCAGAGAATACGATTCTTCAAATGAGATGACAGATTTTAAGAAACTTATAGATGAACATATTTCCAAGCTTGAAGCGGATTCC CCTACGGTCACCTSEQ ID NO:93 Late LPZ-015AGGTGACCGTAAAATACTATGAGAAATGCTTTCATCAGGCACCGCTGGTAGGTTTTCTTCAAGCTTTTCATTAGGCAAAAGAGGCTCCGTGAGTTGATCGTAATCTCTCCTTGAATGGCCATATTGACCAGACACTCTGATTAGAAACTGGAATACAACTGCACATATAGTCATTCTTATATGATCATCCTTCTGCACTTCAGCATCCTGCGGCAACTCTCATCCCGCCATACTGCAGAAAAATTATTTGACTCTTGATCATGTTGTAGATGAATCTTCATGAATCTTCTCATCTTGCATTCTTGTCTTTATATCTTTAGGAAATTGCATCTGGTAAAAGTATAAATGCATCTTCACTGGTTGCTTCAGTTTTGCATGCTCCTGTCTTCTTGTTTACATGTGATCTACCAAATCATCTAATGTATTCTCTCAATGTCTTGTGGACATCTCCTTCATTCCGAGATTACCAATCATCTACCCGAATAAATGTGCCCCGTCAGCAATGCC GTTTTGGTCC SEQID NO:94 Late LPZ-016AGGTGACCGTAGTAGGCGTCCAGAGGCTGACAAAATCCCAGGCCTGTGCAAATCTGGAAGCCGCATGCAGGGCCGTGGCACCTACACTTGCGGCCTTAACAAAGTGGCCCGCGGCACCCACTTCTACCAGTGTGTTTATATTCTTGTGCAGCCAACACCAGAGGTTATGCAGGCGAATGTGCTGGCCAAGCGTTGTTTCGGCTGTCCGCAAACCCTCTCGAGTCTTACATGCCGCATATGAGTCTTGTGTATGGCGATTTGCCTGACGACGAGAAAGAGAAGGCCAAGGTTAAGGCGCAGCTAAATTCGATGAACTTATCCGCAACACGGATTCCAAGTCTCCAGCTTGTGCTTGTACTCGACAGATCTGAAAATAATCCTCACTCATGCATAAGTGCAAAATGTGATCTTAACCTGCTCTGAAAATTACATAA SEQ ID NO:95 LateLPZ-017 AGGTGACCGTCCACGAGAATTTGGCTTCAAAACCCTAGGAGAGGGATATGAACTTGCCAAGGCACAACTGACGCATGAACAAGACGTAAAATGACTCATTAGACACTGACATGATAATGAAAAACCTATGAATGATGATAGACTCAGCTACTTGATGACATCGCCCGCCATTTGGACATCTTTATAAGGAGTTTAAGCAAACCCTAGACCTACTGCCTAGTGACCAACTTTTGCTTGACGACTCACTGAAATGACAATATTTGACCTTGACACTTCAAAATCACTTTGTAGGAACTCATTTGATCACTGGAGGACGGCTGGAAAGACTGACACTAACAGGACTTTATATATGCACCTCGTCTATCCGAACTT SEQ ID NO:96 Late LPZ-018AGGTGACCGTAAGCACAAGTCGTCAAAATTATCTCTATTCCGGCAGTAAAAACCTATAGCTAATGATGGATCAATAGCACTAAGTGGCAGCTGGCGTACATCACTGCAATGATAAGAACCAGTATCAACCCCCATATTATCAGGAGATATCTCCACCACCTGCTGCACTACATGTGGATCTAAGTACAGAGCCTGATCATCCTGAACACCAACAATATACGTTGGCTCCAGGCTTTCCACCAGCAATACCAAGACTTTGGGGAAATGTGAACGTTTCACGAAGTGATGGTACATACCTGGGTTGATCTCTCTACACCAAGAACAAGCGGCACCAAAATCAGGATAGGCACTTGGTCTTCCCCTTCTCCATTGGACCACTCTGAACACAGCCTCGCAGCATCATCAATGCAGATAACTGGAGTCCCTCCACGGTCACCT SEQ ID NO:97 MiddleLPZ-019 AGGTGACCGTGAATATGGTGGGTATTTGCAGGGCAAGATTCAGGATGCTGCTCCCGGAGCTTAAGTAAGGTCTTGGACCCTAATAAATTCAGGGTATATGCATTATGTATATGCTCTCATTTAGCTGCTCATCTGATTTCCATTGGGTGAATCAGTTGTTTTGCAGTACGTGGGGGTCTGTTATTTTGTGAGTTATGGTGGAGTCATTTTGTTGTTGTTGTTTTTCTTATCTAGGGTTTAGGGTTTTGCCCTGTAATCGGTCTTCCCCTCTCTCCTGCGCTTGAATTTGACCTGAAACCTCTTGAAGTAGGCCCTGGTTTTCTGGGCTTTGACGAAAACCATGGTTGTGGATCTCCTCTCTCCTGCTACGGTCACCT SEQ ID NO:98 Late LPZ-020AGGTGACCGTCCTACTTCACCGCAGTGACTTCCATCTGGTTTTAGGAAACTATCCCTAAATCCTTCACTAGTTGACGAATTGATTGACTCAAATCAACTGTCGGTCAAACCCACTCTCTCTGAAAGTGAATTCTATGAGTCTATACCCAACCCAAATCAATAGGTTGAGGTAACAGTTGACCCGATTTCACCTTCAACAAATCATACCTTTCCCGAAGAGAGTGAACATGATTCAACACAAGTTCTTTTTGGTTCACCAGATTCAAATGAGCTTGGGGGTAATCCTCCTGTTCCATCAAGACAAGAAGAAAATCCTCCCACTCTCGTAACTCAAGGGTTAATCCTCCCATTTCTACGGTCACCT SEQ ID NO:99 Late LPZ-022AGGTGACCGTCNCGGGATAGNTGGAGCCNAACAAAGTACNGAANAAANTGAANCGCNCTGGGAAGCGNGCNGAAANNTGGNCANACNTGCCCTNCNACTCGGTTACCCAGCCNTTCTCTACCNANAATTATNACNNNANAGCNCCATGCTGGGTTTGTNANAAAANAACNGCTNTTGATAAAATTACATAGANTNNNGAACACGTTAAGAGGAATATGGTCCANATNCATTNTNAATNANNANTTAAAAACTNNNTATGTNCTAGNGTCNCCT SEQ ID NO:100 LateLPZ-023 AGGTGACCGTACAGCACAGGTATACAAATCATAGAAATGGGCTTCTGTCCAACTGTCAGCAGAAGCGATATGAAACCCAGAAGCATCAACTCTGCTTTCAATTTTTCAAGCGCTTCATATAGAGCCTTTTTATTTCTCTGGAGAGCCAATTGCTAGCATAATGAATACCATGTTCAAGAAGTAAAGAGATGACCACAAATGCCAAACAAACAACTGCTACTGCCCAAGTTAGGAGTTTGCTCTAGAGAACGGTCATTGCCACGGTCACCT SEQ ID NO:101 M,L LPZ-024AGGTGACCGTGGATATGGGAGCAGAGCCGTCCGCAGTGGATGCTGCAATTCAACTTGAAGTGGCAGAAGCTGTGAAGACTCTCCAAATGGACAAGGCACGAAGACAAAACCAAGACAAGGATGAGGGCAAGAGTGGCAACGCTGATTCAGATGACTTGAATGAAATGGAAGTCAAAGCTAAAGCAGCCGAACAACTGCTTGCTGTGCATGGGGCAGCATTACTACAGAATGCTCTGAAAGAAAATTGTCGAGTCATGAAATGCGGGTTGGTTCAAATACAAGGGAGGAAGGTGAAGTTAGAAAGAACAGAAAGGGCATCAACGCAGACCCCTCACTGATATCGGCAACACTACGGTCACCTAAGCCAATTCTGCAAATTTCCATCACTGGCGGGGCCCGCTCCAACTTCCTCTAAAAGGCCAATTCCCCTATATGATTCTTATTACAATCCCTGGCCCTCCTTTTCCACTTCT SEQ ID NO:102 M,L LPZ-025AGGTGACCGTAGCAGGAGAGAGGAGATCCACAACCATGGTTTTCGTCAAAGCCCAGAAAACCAGGGCCTACTTCAAGAGGTTTCAGGTCAAATTCAAGCGCAGGAGAGAGGGGAAGACCGATTACAGGGCAAGGATCCGCCTGATTAACCAAGATAAGAACAAGTCAACACACCCTTGCCAAAAAAAAAAAAAAAA SEQ ID NO:103 Middle LPZ-026AGGTGACCGTATGAGCAAGGAGGGAACAGTATGACAGGCAGTCAAAGCCCACGAGGGGTGCCCCACTGCCTGCAGCAGCGCACTTACTTGGACTAACAAACTTGTATCGTGATTAAAACGATGAACATCGTATTGTGGAGTGGAGCCACTCGTGACCTGATTCTGTCCTAAGTACTTGGTCCTGGAATACAATATTGCACGGTCACCT SEQ ID NO:104 All LPZ-028AGGTGACCGTCAAAGTACAATGGAGTCATATATCCACTTGAATTGAAACCTCTAATTTAAAAGTTCTCAAAAAATATTTTATTTACAAAACAGGGAAAATAAAAAATGACTCTATCAACTATACAATCCTAACATCCATCTCCCGACAGACCTCCAGTATATGTACAAGGCGCTGAAAGAAGGCTGATTATTTTCTATTCCAGCTCGCATAACGTGGTCTTCTGAGGCTTTGCCTATTCCTTTCTTTAAAATCTTTCGCACGAAAGATTGGCATTGACCTTCGGCTAAATCTCAGACTCCAGGGAACCTTGGACTCCCTTTAAAACCTAGAGCTACTTTTTACGAACCCCTGCTTCTCTTGAACACTTAGGGAACTTATACTTACAAAACTTCGGGAACTCCACCCCCTAGCTTTGCAGGACTCCAGCAGATTCCCCAAACTGCCAGAAGGCTATTTCCATGCACTGTTAGGGGTGAATTCCTACTATCAAAACCCCCAAAACATCATA SEQ ID NO:105Late LPZ-029 AGGTGACCGTATGGGAACAAGTATGGGAACAAGAACGTATTACATAAAAGATGGAGATGCAACACAGCATAAATTGATGCTAAGTTGTTACAATGATGCATACAGCTTAACCAAGCTTGGAATGACATCATTAAGTGCGGTCACAGCCTCTGCATAGTATTTCTCTGCCTTGGGTGTATCCTTGCTCCTTGCAGCGTAGTCCAGGTTGTCAAGGGTTGTCAAAAAGCTTGGTGGTGAAGGTTTTGAGGGGCTTCTTCTGGTCCTTGGGCTTTGAGGAGATAACGGTGTTTGAAGTCCTTAGCGAAAGTAAGAAACCTTTGGAACCGAAGTCCGTTCTTGACGTTACCGCACGCCTTCCTTATCTATCACTTTTTCACCTCCAGAAATTGCTTCCCGAATCCCTTGCTCTCCCACCCCCTGTCCCCC SEQ ID NO:106 Late LPZ-030AGGTGACCGTAGTGTTGCCGATATCAGTGAGGGGTCTGCGTTGATGCCCTTTCTGTTCTTCTACTTCACCCTCCTCTCTTGTATTTGAACCAACCCGCATTTCATGACTCGACAAATTTTCTTTCAGAGCATTCTGTAGTAATGCTGCCCCATGCACAGCAAGCAGTTGTTCGGCTGCTTTAGCTTTGACTTCCATTTCATTCAAGTCATCTGAATCAGCGTTGCCACTCTTGCCCTCATCCTTGTCTTGGTTTTGTCTTCCGTGCCTTGTCCATTTGGAGAGTCTTCACAGCTTCTGCCACTTCAATTGAATTGCAGCATCCACTTGCGGAACGGTCTGCTCCCCATATCACGGCACCTT SEQ ID NO:107 Late LPZ-031AGGTGACCGTAGTGTTGCCGATATCAGTGAGGGGTCTGCGTTGATGCCCTTTCTGTTCTTCTACTTCACCCTCCTCTCTTGTATTTGAACCAACCCGCATTTCATGACTCGACAAATTTTCTTTCAGAGCATTCTGTAGTAATGCTGCCCCATGCACAGCAAGCAGTTGTTCGGCTGCTTTAGCTTTGACTTCCATTTCATTCTAAGTCATCTGAATCAGTGTTGCCACTCTTGCCCTCATCCTTGTCTTGGTTTTGTCTTCGTGCCTTGTCCATTTGGAGAGTCTTCACAGCTTCTGCCACTTCAATTTGAATTGCAGCATCCACTGCGGACGGCTCTGCTCCCATATCCACGGTCACCT SEQ ID NO:108 Late LPZ-032AGGTGACCGTCGTGAAATAGCGAGAACGGCGTGGAACATCGCAACGGCGGGGAGGCTGGCGGACGTTGCACGTTTCTGGAAGGTATGCGGCTCTCTCCTCCGCCTCAGTTTCCATGAAGAGGTCCTCCCTGGTGAATCATACGATTGCGATTGATCGAGTACTTGCTGTATGGCTCGGCATCGGCATTGTGGAGACATTCTTTCCTATTCCTCGCAGCATCTCTCCGATGGTTGCTCTCTCCGGAGCTCCATGTTATCCCCGGCACTGAGACAGTCGCTGCCGAATCGCAAGAGCTTCTTTGTTTTTTGCAGGCTTCTCCAAACATAATGCCTCCGGGCCCCTCAACCGAATTCTGCCAAATCCACCCC SEQ ID NO:109 E,L LPZ-033AGGTGACCGTGGACGACAGTGAGTGCAGTCATCATGCTCTCCAGTGGACTTTAAGCAATCTGCATCTTTATGGAAGTGATGTATCTCTTGTGGTTTTTCATGCTCAACCATTGGCAGTCTTCAACAGTGCTGCAACAATGGGCATAACGTCTCCCGAATTAATTGAAACTATTGTGAATCAACAGATAGGTTTCTGGTCACATCTAGCAATACAAACACAAATAACTGTGGAACAGAGCCACAAAACTATGCTTCAGAGCATCTAATTACACATATCTCTCTAAAACCCTTGCATAAAAAATAAACTGAATCTCGACCTTAGCACTATTGCCACCATCATCTCAAGCAAACATTCTCTAGAATACCATCTTCACAATGCACTAAAGTTACATAAGCACTGAACTTAAAACATTTCTGTGACGAATGAAGGACCAATTCATCATACTCAGCCTTTGCATCCAATCTGTTGAATGTGCTGAAAAATGCCCAATAAACCTCCATCCAACACTGTCTTCCTCTCTGAGGTGCACACTGATTTCTGCTGCTGAACCAGTCGGGATTCCCT GCTCAACGTCCCSEQ ID NO:110 Middle LPZ-034AGGTGCCCGTGGAACTACTGTTAAATCTGGAATCCCTTGTCTAGCTGTTAAAAACTCGACAAGTGCATGTTGGTATAGTAGGGTAACAGAAGGGTTCTTACCCAGATTTACCCCTTTGGCGGAGATATTTAAAAAAAAAGAATTGTCATTATGGTAAATAGGTGTGACAGGTTATCAATAGAATAACTGACGAGAGTAAACTGATAATTATTAAGGTTAAAGTGTTCGTAAAGGAGACTTGGACTCTAGGTTGGATGCCTACACTTAGAGCCGTCCCGCA CTTGGACGGTCACCTSEQ ID NO:111 Middle LPZ-035AGGTGACCGTCCAGTGCGGGAACGGCTCTAAGTGTAGGCATCCACCTAGAGTCCAAGTCTCCTTTACGAACACTTTAACCTTAATAATTATCAGTTTACTCTCGTCAGTTATTCTATTGATAACCTGTCACACCTATTTACCATAATGACAATTCTTTTTTTTTAAATATCTCCGCCAAAGGGGTAAATCTGGGTAAGAACCCTTCTGTTAACCCTACTAATACCAACATGCACTTGTCGAGTTTTTACAGCTAGACAAGGGATTCCAGATTTAACAGTAGTTCC ACGGTCACCT SEQID NO:112 Late LPZ-037AGGTGACCGTATGGGAACAAGAACGTTATTACATAAAAGATGGAGATGCAACACAGCATAAATTGATGCTAAGTTTGTTACAATGATGCATACAGCTTAACCAAGCTTGGAAATGACATCATTAAGTGCGGTCACAGCCTCTGCATAGTATTTCTCTGCCTTGGGTGTATCCTTGCTCCTTGCAGCGTAGTCCAAGTTGTCAAGGGTGTCAAAAAACTTGGTGGTGAAGGTTTTGAAGGGCTTCTTCTGGTCCTTGGGCTTTGAAGAAATAACGGTGTTGAAGTCCTTACCAAAGGTTAATAAACCTTTGGAGCCGAAGTCGTTCTGGACGTACGGCCACCCCTTCCTTATCTATCAGCTTTTTCACCTCCAAGAATTTGCTTCCCCGAATTCCTTGCTCTCCCAGCCGCCTGGTCCCCCGAAAAGGGCTGAATATAAAACCGTCCTCAACGGCATTCCATTCCTCCCTCGTCTGAAACACTTCCCCGCTGCCCCCGAGGTGAAGGGCCATCAACTTGATGAACGGCTTTTGCAAGGCTCTGACCCCGGCCCCGTCACT AACCAATTCTGCAATCSEQ ID NO:113 Middle LPZ-038AGGTGACCGTGGGGAACAACTACATGACAAATCATTTCTTTGTGGTGGATGTACTGGACACCAAATAAGTGTTGAGAGTCCACTGGCTCTGTACGCGTGGCAGAATCACAACGGACTTGAGAAAGTTGAAGATGGAATTTGTATCGCTAGATGGCCAGACCATGTTGCTTCAAGGGATGCACTCGTAACCCCCACAGTCTGTCTCTACCCACTAGATGGAGGCTGACATGAGACATGGAGACATTAATTGGGTTGTGGAGTTAAAGATCTCTCACGTTCGGGGAAAATCCAAGCCATCATACTTATATATCCGTCCCGTGCATGTAACCTCCTCCACTCTGTCCCTTAGGCCCGTTGTTGCCT SEQ ID NO:114 E,L LPZ-039AGGTGACCGTATGAGCAAGGAAANNACCGCACTGGCTCCCAGCAGCATGAACANCCAGGTCCCAACCATANACCNCNTGGAGAANGTGATCAAGATATTAGCGACAGTGTNATTGTACNTCTCNCCAAACACATTATACACGATAAGAGAGCNTAAACTACTCTATTCCTTTGACGNAGTGACTACNTGAGTANAAGCGATCATTATCTTGCNAACTTTGCATGAAAACAACAAACCCACNTCCAGTTTCTCTATANTCTGGCCCCACNATGAATAANANTCCTGCCATAATAATGANTCTTTGTCCCCANAGANAAATTNNATAAGACAGGAGCCCACTGTTGCTTGCATGACTACCANTCACTTTAAGGCGTTGCGAATCCCGGTCCTAACCATCTCCATACCATNGGCANNCTTTACTTTCCAACTGCCCAAGACTGTGAACAGGGCGGTTCNNACCCTATAANTTTTAGCCTCTNNTCGAANCNCTTNTTTTCGTTCCCCGGAAANCCGNTTCCCACCCTTTGGAACCTTTTTTTTTTGCCGGGCCCCAGGCNAATTCTNCAATTCCCCNCTGGGGGG SEQ ID NO:115 Late LPZ-040AGGTGACCGTGGCGGAGGTTAGGGAAGTTTGACTTCTCATTTTCTCACGCACTCCTCTCCCTCGTAACCTCGGTCGAGTCGATGGCGGCTTTTTAGTCGAGTGTGCTAACGCACCCTCCGGGCCTCAAAATTTCCAGCTACTCGTATTTGATCAATGCTGAAATCGCGTAATCACGTAGATAATAAAGCGTAATGAATTCTATAATGAAGCATGTTTCTCTATAGTTCATGTTGCCGAGAAGGAATAATGAAAATGAAGCCTTATATATTATCTGGGGCTCAAGGAGATGTTATCTTTTCTCTTCCTTGGTTAGAGACCGTCACCTTCACTTTGAATTGGATAAAGCTTCATTTGTTTAAGACCTCCCACCCGTAAATACATACGGTAGCCTTCTTATGTTAGAAACATACGTCACCTACGCAGAATTGTTAGAATGAAATGA SEQ ID NO:116 LateLPZ-041 AGGTGACCGTGGAACAAGATGATTAGTTCTCATGCGGGCCAGGATGATTAGTTCTCCTATGGCAACTGTTGGACAGGATGATTCGTTCTCCTGTGGACAGGATGATTAGTTCTCCTATCGAGGCATCCTACCCAAGCAGTTTGGGACTCATGGGAAGTACCTCTCATCTGATCAATGAGTAGGAAATGGGGTTAGGGACCATTAAGTAGTATTATCGATGGATGCATTGTTGTATCTATTGTACTCCCTATGCTAGAATGAACTCCATTGATCTGGGATCAATGAATACTGTTTCTGGGAATCATTGAAAATTTGTATGAACACACTCTGAACACTGAATTTCCGGTTCATTGGAAGAGATGGTTTTAAACACTCTCCTCATCTCATTTCTTCCCCTTCCTTATTCCAACCAAATTTGGGCCACCCTGCCAGGAAATTCATTTGATGGTTGGAAAATACCACGGGCCCTAACCAATTCTGCAA SEQ ID NO:117 Late LPZ-042AGGTGACCGTNCATCTCTACCATNATNCCTCCCTCCCGNCTGTATCANCNGGCNTNNANGTCNTTNNCTANNNNAAGNTAATCCTATCCCNTTANAGTGACGGTCTCTANNCCTAGAAGAGAANCCATAACATCTCCTTGAGCNACACATGGGATATACCGCCANCTTATNTAATACTTTCNCNGCACGGTAACNGACCANAANCATTCTTCACTATAGAATTCATGTCGCTTCATTATCTACCTCATTNCNCCANATCCCCCTTNATCTCATNNATTTACTAGAAANTTCTGAAGNTCCNNAAGGGTTCGTTTTGCACCNCCCCAANTAAAAAANCCCTNCCGNTTACNTCGAACGAAGGTTTCAAANGAACAGNAATTCCTTTACAAAAATCAANAATTTTAACTTCCCNAATCCGGCCCCCCNGTNCCCGAAACCCNATTTCTACGATTGCATCACCCCGGGGGNCCNCTCAANCCNNCTCTAAAGGNCCATNCCCNTNNNTGATCCTCTNCCATCCAANGGCNCCTTTCCACTTTTATTGGAAAACCCCCNTTCCCCNTTTTACCCTTNNAAGGCCCCTCCC SEQ ID NO:118 Late LPZ-043AGGTGACCGTGGAACTACTGTTAAATCTGGAATCCCTTGTCTAGCTGTAAAAACTCGACAAGTGCATGTTGGTATTAGTAGGGTTAACAGAAGGGTTCTTACCCAGATTTACCCCTTTGGCGGAGATATTTAAAAAAAAAGAATTGTCATTATGGTAAATAGGTGTGACAGGTTATCAATAGAATAACTGACGAGAGTAAACTGATAATTATTAAGGTTAAAGTGTTCGTAAAGGANACTTGGACTCTAGGTTGGATGCCTACACTTAGAGCCCGTTCCCGC ACTGGACGGTCACCTSEQ ID NO:119 Late LPZ-045AGGTGACCGTGGGGGATGGGGCCGTGGGGAAGACTGTATGCTCATCTCCTACACAAGCAACACGTTTCCAACGGATTACGTGCCGACTGTTTTTGACAATTTTAGTGCAAATGTGGTGTTGATGGCAATACAGTAAACCTTGGCTTGTGGGACACTGCAGGGCAAGAAGATTACAACAGACTGAGGCCATTGAGTTATAGAGGTGCAGATGCTTTTCTGCTTGCCTTTTCTCTGATCAGCAAGGCTAGTTATGAAAATATATCAAAGAAGTGGATTCCAGAACTAGACATTATGCACCAAATGTGCCAATCATTCTTGTGGGAACTAAATTAGATTTGCGTGATGACAAGCAGTTCTTTGCTGATCATCCTGGAGCAGCCCCTATAACAACAGCTCAAGGTGAAGAGTGAAGAAGCAGATTGGAGCAGCAGCATATATTGAGTGCAGTTCCAAAACCCAGCAGAATGTCAAGGCTGTTTTTGATGCTGCTGCAATTAAAGTGGTTCTTCAGCCACCAAAGCAGAAAAAGCGGAGAAAAAAGCAGAAAAATTGTTCTATTCTCTAAGAAAAATGTGGATGTTCTGAACGCNCTTCACTGACAATAANGNTGACGTNG GAATATCTTCCTCCSEQ ID NO:120 Late LPZ-047AGGTGACCGTAAGCACAAGTCGTCAAAATATCTCTATTCCGGCAGTAAAAACCTATAGCTAATGATGGATCAATACCACTAAGTGGCAGCTGGCGTACATCTCTGCAATGATAAGAACCAGTATCAGTCCCCATATAATCAGGAGATATCTCCAGCACCTGCTGCACTACATGTGGATCTTAGTACAGAGCCTGATCATCCTGAACACCAACAATATACGTTGAAGCTCCGGGCTTTCCACCAGCAATACCAAGACTTTGGGGAAATGTGAACGTTTCACGAAGTGATGGTACATACCTTGGGTTGATCTTCTCTACACCAAGAACAAGCGGCACCAAAATCAGGATAGGCACTTGGTCTTCCCCTTCTCCATTGGACCACTCTGAACACAAGCCTCGCAGCATCATCAATGCAGATAACTGGGCGCCCTCCACGGTCACTT SEQ ID NO:121 LateLPZ-049 AGGTGACCGTGCCATAGCGCATGGCGTGTAACTGGATGAGACCGCATGGCTCAAATCTGCTAGGAATCAACATGAAATCAGCTCCAGCTGTTATCATATGAGCAAGTGGCACGTTAAACTTTGCTACTCCCCTGACGTTGTCTGGATATTTCTCTTCAAGCTCTTCAAGCTGCTTCTCCAAGTACTTTTTACCGGTGCCTAGGATAATTAACTGCACGTTTCATCTGCAATTAGAGGGACAGCTTCAGCAAGAATATCTGGACCTTTCTGCTCTTCAAGTCTTCCAATAAATCCTATAACAGGAATATCTGGATCCACGGTCACCT SEQ ID NO:122 EarlyLPZ-051 ATGTGACCGTCAAAAGGGCATATAAATCGGGGAGCTCAATGGCAAGAATGTACGATTTCTGGCCTCAAGTCGCCCTGAATTTGGTCAACAACATCTTGATAGAGCGAGAGGACGCTCCCAATTAAGATCTGGAAACTGTCGAGAGTGATTGAGGTCATTTTTAATCTAAACTGAATTGTGGGGACAATTTTTCAATTCAGATCCTTCTAGCAAAGCAAAGCAAACCTTAACAGTATTGTATCCATGAGAATGGATTCTGCACAGGTCAGGCTCCACGGTCAC CT SEQ IDNO:123 All LPZ-053AGGTGACCGTGGAGAAGAGAACGCTTTGCCGACTCTCTGGGATGCCCTTCCCTCCATAGCCGTCGTGGGAGGACAGAGCTCCGGGAAATCCTCTGTGCTGGAGAGCATCGTTGGAAGGGATTTTTTACCGCGTGGATCAGGTATTGTTACTAGACGGCCGCTTGTCCTTCAACTTCACAAGACTGATGAAGGCAGCAGGGATTACGCCGAATTCCTTCACCAACCCAGAAAGAAATACACCGACTTTGCACTGGTAAGGAAGGAAATTGCGGATGAGACTGATCGAATTACAGGGCGTTCCAAGCAAGTCTCAAGTGTCCCAATCACCTTAGTATTTATTCACCCAATGTTTGTAAATTTGACTCTAATTGATCTCCCTGGGTTGACAAAAGTGGCTATTGACGGTCACCT SEQ ID NO:124 Middle LPZ-054AGGTGACCGTGCAATATTGTATCCAGGACCAAGTACTAGGACAGAATCAGGTTACGAGTGGCTCCACTCCACAATACGATGTTCATCGTTTGATCACAATACAGGTTTGTTAGTCCAAGTAGGTGCGCTGCTGCAGACAGTGGGGCAGCCCTCGTGGGCTTGGACTGCCTGTCATACTGTTCTCTCCTTGCTTCAGGCTCTACTGCTGTTGCTGCTGCTG ATACGGTCACCTSEQ ID NO:125 Middle LPZ-055AGGTGACCGTACATACAAGGTCTTATCACCAGCAGCAAGAATAATCAGTTGGCCATCTTCTGCAGGCTTCTTGCTGCCTGAGACAGGAGCCTCAAGAAATTTTCCCCCCTTTTCAATGATTGCCTCATTGATCTTTGTTGAAGTGATAGTATCAACTGTTGACATGTCAATGTATCCTTTTCCTGTACACATTTGCTCTAGGACACCATCCGAGAGGGCAGCAGGAGGATCAGACAGGATGGCTATGGTATAGTTGCACTTCTTTACAACTTCGGCAGGAGTGCTTCCTATGGAAGCACCTTGCTGAACAAGTTCTTCACACCTAGACATTGTCCTATTCCACACGGTCACCT SEQ ID NO:126 Late LPZ-056GGTGACCGTACATACAAGGTCTTATCACCAGCAGCAAGAATAATCAGTTGGCCATCTTCTGCAGGCTTCTGGCTGCCTGAGACAGGAGCCTCATGAAATCTTCCCCCCTTTTCAATGATTGCCTCATTGATCTTTGTTGAAATGATAATATCAACTGTTGACATGTCAATGTATCCTTTGTCCTGTACACATTTGCTCTAGGACACCATCCGAGAGGGCAGCAGGAGGATCAGACAGGATGGCTATGGTATAGTCGCACTTCTTTACAACTTCGGCAGGAGTGCTTCCTATGGAAGCACCTTGCTGAACAAAGTTCTTCACACCTAGACATTTGTCCTATTCCGCACGGTCACCT SEQ ID NO:127 Late LPZ-057AGGTGACCGTGGAGGGGCTCCAGTTATCTGCATTGATGATGCTGCGAGGCTGTGTTCAGAGTGGTCCAATGGAGAAGGGGAAGACCAAGTGCCTATCCTGATTTTGGTGCCGCTTGTTCTTGGTGTAGAGAAGATCAACCCAAGGTATGTACCATCACTCGTGAAACGTTCACATTTCCCCAAAGTCTTGGTATTGCTGGTGGAAAGCCTGGAGCTTCAACGTATATTGTTGGTGTTCAGGATGATCAGGCTCTGTACTTAGATCCACATGTAGTGCAGCAGGTGGTGGAGATATCTCCTGATAATATGGGGGTTGATACTGGTTCTTATCATTGCAGTGATGTTCGCCACTGCCACTTAATGCTATTGATCCATCATTAGCTATAGGTTTTTACTGCCCGGAATAGAAATAATTTTGACAACTTGTGCTTACGGCACCT SEQ ID NO:128 LateLPZ-058 AGGTGACCGTGGAGGGGCTCCAGTTATCTGCATTGATGATGCTGCGAGGCTGTGTTCAGAGTGGTCCAATGGAGAAGGGGAAGACCAAGTGCCTATCCTGATTTTGGTGCCGCTGTTCTTGGTGTAGAGAAGATCAACCCAAGGTATGTACCATCACTTCGTGAAACGTTCACATTTCCCCAAAGTCTTGGTATTGCTGGTGGAAAGCCTGGAGCTTCAACGTATATTGTTGGTGTTCAGGATGATCAGGCTCTGTACTTAGATCCACATGTAGTGCAGCAGGTTGGTGGAGATATCTCCTGATAATATGGGGGTTGATACTGGTTCTTATCATTGCAGTGATGTACCCACTGCCACTTAGTGCTATTGATCCATCATTTAGCTATAGGTTTTGCAGTGATGTACCCACTGCCACTTAGTGCTATTGATCCATCATTAGCTATAGGTTTTACTGCCGGAATAGAAAAATTTTGACAACTTGTGCTTACGGTCCCT SEQ ID NO:129 LateLPZ-059 AGGTGACCGTGCTAGGACACACAATTTCTCAGCAAGGATTACAGGTGGATCCTAACAATATTGCTATAATTCAAAAGGTTCCACCTCCTTAAAAGGTAAGAGATGTTTGGAGTTTTCTAGGCTTGGCAGGATATTATAGAAGATTCATCAAAGATTTCATTAAGCTAGCCTCGCCATTGTCTAGCCTCTTAGGGAAAGATGTTGAGTTTCAATGGACTGATGACTGCCAAGGGGCTCTGGATGAGTTGAGAGATAAGCTGGTATCCGCCCCGATCTTGAGAGGTCTAAACTGGGCCCTACCTTTCCACATCCACATTGATGCCTCGAACAAAGCCATAGGGGCAGCCTTAGGACAAGTTGAAGAGAAAATACCATATGCCATATACTTGTCAGCAAAAATCTGTCTAAGGCAGAACTGAACTATACGGTCACT SEQ ID NO:130 Late LPZ-060AGGTGACCGTCATATTCCCCTCTATAGCAGCACTAACAATCCATTTTCTGAGTGCATCAGAAAATCAACACACGGTAAATGTCTTGAGACTAACGAGAAATTAATAATCACGTTGTACAAAGAACAGTATGTCCCGTCACGTCACGAGTGCCCTGAGAGATCATCCAACTTTCTCTGAACCCTCGTGTTACACGCACGCAAAATCAAGGATCAGTTGTAGTTATTGCTGGCGTGACAGACGTGACACCTACTGTTCCGCTACAAACGATATAATTGAATCCATGATCGGATTATGTATTATGATCTTAGCGCAGTGGTTATGAAATTATGATGAATTTGCTTATGATTTTCTCAGCGTTTGTGGAAGAATCTCGCTATTGAAAACTTCCCCGTATATTTCCAAACTTATTATCATCCCACGGTCCCT SEQ ID NO:131 Late LPZ-061AGGTGACCGTACAGCATTTATTGATGTTCTATTTTGTTGTTTGCAAGTTTTTCCGACGCTGTGAGGCACGGAAAACGAGATAAGTTGTAAAAGTTTGCTCGCTGATTTGAGGCACGGAAAACGAGATAAGTTGTAAAATTTTGCTCGCTGATTTTTTGCTGAATATTTCTCTCACTATAAAAAGCATTTTCCAGAAATAAGAAGGAGCTTTCGAACTGGTTTTCCCCAAGAGTTGTAGGGGGTTTTTCCACGGTCACCT SEQ ID NO:132 Late LPZ-062AGGTGACCGTATTTATGGTCGCAGGCACAAATTCTGCTACTGTAGAAGGGTTCTTACCAACTTTAGGTAGAAGGCGAGGAGGGCTTTATTAGTACAGTTCTGTGTAATCTTAATGATATTTTTTGCACTATTATTTTATGGTAAAAGGATTGATTTGTCTTTTGCAAAGGCCTTAGGATTGTTTATTTACCTTTGGGCTAAGGGAGGAGGTAAATTTTTCACATTGGGAAAAAAAATGCCTCGGTCGTTGTCACGGTCACCT SEQ ID NO:133 Late LPZ-063AGGTGACCGTGCCAGTATGACAGATGGAACCATGCAGCTAGCCACCAAATTGTAAACATCAAATTTTGTCTCAATATAAGTTGCAAATTCTTAATTAATTATGATCACCATTTC AACGGTCACCTSEQ ID NO:134 Middle LPZ-065AGGTGACCGTGAATAGAAGCGAACACATCCTTGTTGCTGAATCTAACGACCAATCGGTATTTGGGTGTGTTGTACTTGTTCTTATCTTGGTTAATCAGGCGGATCCTTGCCCTGTAATCGGTCTTCCCCTCTCTCCTGCGCTGAATTTGACCTGAAACCTCTTGAAGTAGGCCCTGGTTTTCTGGGCTTTGACGAAAACCATGGTTGTGGATCTCCTCTCTCCTG CTACGGTCACCTSEQ ID NO:135 Middle LPZ-066AGGTGACCGTGGTAGAGGAGGCAGGCACTCATCTAACAGTCGAAAGCCCTTTACAAAGGGGAATGGTACCAGCATAGAGAAGAAACACAGACGGTTTGAAGAGGATGATGGATCTGCCATAGATGAACGATCAAATAAGGTTCAAAAGCTGGAAAATGATGGTGAATTCCATGCATCCCACTTGGCTCTGTCCCTCAAGTTGAATATACCTGGACGAGAGGTATTGCATTTCCCAACGGTCACCT SEQ ID NO:136 Middle LPZ-067AGGTGACCGTACTGATAATAGAAGAGGCAGGGAAAGAGAAATCAATGATAATAGAAGAGGCAGGGAAAGGGAGATCAATGGCATCATGCTACTTCTGTAGCTGTTACCTTAGTGATGTAATCTTCCATGGCAGACTCGGGGGTTTTATCTTTAAGTTGAATTTCCATGCATCCCCTTGGGCTCTGTCCTCCAGTTGAATATCCTGGAACAAGAGGTTTTGCT TTCCACGGTCCCCTSEQ ID NO:137 Late LPZ-069AGGTGACCGTGAGAAGGCAACTTTATCCCCTGCTAAACCAAGTCCAGAAATGAGGAAAATATGTGAAAACTGAATTGCTATATATGATGCCTAGTCTTGGCCTCTCAATTACAAGTTCAACGTCTTCAAATGATTGAAATATGGACCTTCTTAACCGTTCTGGAAATCTATCAATCTTCAAAATTTTGAAACTTTGCCTCGATCTTGGAGTGATCAGACTTGATTTCTAATCCTAGAAATACCCTATCACTGGCTACCTGGTCTGTACGGTCACCT SEQ ID NO:138 LateLPZ-070 GGTGACCGTGGGATAGGCAGAAGCAAGAAACACAGAAGTTCTCCGGGAATGTAAGCGCTGACAGTGGGGGAGAAAGTAGTGAACAAGGACATGGTCGGTATGAAATACATGGCAGGCGATGGATTTCAAGGGATTAAGCATCTCAATGGATATTTACTATTGGACTGTAGTAACTTTCGCCATCGCTTTTTGAACACATCTGTGGCTTAACTGTCATCTGTAATGGTAAGCGAACCAGGTTTTGTTCTGAACCACTTGTATGTACGGTCACCT SEQ ID NO:139 LateLPZ-071 AGGTGACCGTGGTGGAGCGATTAGTGATTGTGATAAAGGGAGCATCAATATCTATGTAGACGCCGTATAAAGGTGGAAAAGGTATGTTTTGCAGGTATTTCTTTGTAAATGGTTTATAATGGGTTAAGCTCGGATATATGAGGTTTATATATAAGTCCTGTTAGTGTCAGTCTTACCAGCCTTCCTCCAGTGATCAAATGTGCTCTAACAAAGTGATTTTGAAGTGTCAAGGTCAAATTATGTCATTTCAGTGAGTCTTCAAACAAAATTTGGTCACTAGGCATTAGGTCTAAGGGTTTGCTTGAACTCCCTCTAGAGTTGTCCAAATGGGCGGGCTATGTCATCATTTAAGCTGAATCTATCATCCAATCAATAAGGTTTTTCATTATCATGTCAGTGTCTAAATGAGTCATTTTACCGTCTTGTTCACGGCTTCACTTGTGCCTTTGGCAAATTCAATTCCCTCCTCCAAGGGTTTGAACCAATTCTCTTGGACGGCCCCTAAACCAA ATCTGCAAAATCCACSEQ ID NO:140 Late LPZ-072AGGTGACCGTGGTGGAGCGATTAGTGATTGTGATAAAGGGAGCATCAATATCTATGTAGACGCCGTATAAAGGTGGAAAAGGTATGTTTTGCAGGTATTTCTTTGTAAATGGTTTATAATGGGTTAAGCTCGGATATATGAGGTTTATATATAAGTCCTGTTAGTGTCAGTCTTTCCAGCCTTCCTCCAGTGATCAAATGTGCTCTTACAAAGTGATTTTGAAGTGTCAAGGTCAAATTTTGTCATTTCAGTGAGTCTTCAAGCAAAATTTGGTCACTAGGCATTAGGTCTAAGGTTTGCTTTAACTCCTTCTAAAAGTTGTCCAAATGGCGGGCTATGTCATCATTTACGTCTTGTTCAGCTCAGTGTGCCTGGCAATTCATTCCTCTCTAAGGTTTGAACCATTCTCTTGACGGCACTAAGCCAATCCACACTGGGGCCGTCTATTGAATCAACCCGGACACTGGGTTACAGGCAAC SEQ ID NO:141 Late LPZ-073AGGTGACCGTCCAAGAAGAAATTGGCTTCAAAACCCTAGGAGAGGGAAATGAACTTGCCAAGGCACAACTGAAGCATGAACAAGACGTAAAATGACTCATTAGACACTGACATGATAATGAAAAACCTATGAATGATGATAGACTCAGCTAAATGATGACATAGCCCGCCATTTGGACAAATTTTAGAAGGAGTTAAAGCAAACCTTAGACTTAATGCTTAGTGACCAAATTTTGTTTGAAGACTCACTGAAATGACAAAATTTGACCTTGACACTTCAAAATCACTTTGTAAGAGCACATTTGATCACTGGAGGAAGGCTGGAAAGACTGACACTAACAGGACTTATATATAAACCTCATATATCCGAGCTTAACCCATTATAAACCATTTACAAAGAAATACCTGCAAAACATACCTTTTCCACCTTTATACGGCGTCTACATAGATATTGATGCTCCCTTTATCACAATCACTAATCGCTCCACCACGGTCACCT SEQ ID NO:142 Middle LPZ-074AGGTGACCGTGATAGACCCAAGAAAAATAGATCCAACCCTCAGAGGGACAAAGACTTATAAAGACTAGAAGAGTGAATCAACCTATTCTATTTAGAATATATATTTTTGGGGTGCTTGCTTATCGTTTTGGGGGTTAATGTATGTCGTACTACGGTCTTATGCCCTAATTTGCCCATTGAAATCAACTAAATTGACAGTAACCGACTAAAAGTTGGTCCACACTAAGATATCGATGACCAACGATCATAAAGGTGTCCATGATCCTAATAGTATATGTGTCAATTAATGTAACTTTGGTGCTACAACATAAAACCATTCGTGGGGATCCTCCTTTTTATG CGGTCACCT SEQID NO:143 Middle LPZ-075AGGTGACCGTGGGACCGACCTTGACTACAGGCCAAAATTTTGACTGTTGACCAGCGTTCACTTCTGTATTTTTGGTTGGTATGAGCAACATTGACTTGCTGGAAATTGACCAGGTTTGACTGGTATTTGGACTTGGATTTTGGCACAGATTTCTAGACAATTTGTATTTGTAAACCTTACAGAAGAATAATTTATCGAAGAAGAAAAATGCTAGGTTTCCCCTCAAGTTTGGGTTTCCCAAGGGAAAAATTGTTGTCCCAATGGTTGAATTTTCCAAAGGTCTCCTAACCCGACAATACCTCCTAAGAATTCCTTAATTTAACCTTTCTTGTTTTCACGGT CACCT SEQ IDNO:144 Middle LPZ-076AGGTGACCGTGAAGGAGCAGCAACAATTTGATTTTGTTTGGGTAGATCGGGGATTTTCTCGTGGAACATACCTGATTGAGTATAAACTAAGTCAAGGTACTGTGCTTGAGAAATTACTTGCTCCTCAGTAACTACTCTGGCCTTAGCTACATCCTCAGTGATCTTGGGTAGTAAAGATTTTACAAACCATTCAGCTAAGATCTGATCCGGGATATAAACTTTCACTAAACGTCGTCGACGTCTCCATTCATGGATATGATCTGAAATGTAAGTGGACGTTGACTGCTTTAACGAAGTTAATAATTCTGTGCCATTTTCATATCTGACGGTCACCT SEQ ID NO:145 LateLPZ-077 AGGTGACCGTACCTAATGGGAAGACACTTCAAGGTAAAAACAAATCATGATAGTCTTAAATACCTTTTAGAACAAAGATTATATTCAGAACAACTTGCTGGAAGTGTACCAAGTATGACTGGTATTGAGACTTAGATCTTCGCACAGATTTCAAGACAATTTGTTGTTGTAAGACTCACTCACGAAAAGTGATGTGGATATGAAGAACTTCCCTGTCGCCTCTTGGTTAGGAGTCTCCCACTCATAGGAATTGTGTAACTTATAACTTGGTCCACTAAAGAAGTTAGGTACAGTGTGTTCCTTTACCAGGTTCCCTGTTGTAACTTACAAATCTACGGCT ACCT SEQ IDNO:146 Late LPZ-078AGGTGACCGTCACTGGAGGTTTGAGATGCTTGATCGGTACTGAAATGAGACATGATCAGAATAGGACCTTGTTGAGGCCGTGTCTCACCCCCCATCCACAATCTTTTGTAATTTTGAGTTTCGTTTAGAACATACTTGTAGGATAAAACTTACCTTACTCATGGATCATGGCTGTATATGTTTATCGACCAGAGACAGATATGCCGAATGAAAGCGAGTCTAGTATTCTAATGCAATATATTGGTAGTATGGGACATAGTACTGAACACTTGTATAGTACGG TCACCT SEQ IDNO:147 Late LPZ-079AGGTGACCGTGGTCTCAGTTATGCCATATGTCCGCCCCTCCATATGATGCTCCGCCTCTATGGGGGTCTTTGCGATGTTGATATCTAGTAGTACTTCTTGTCCTATTGCAGCAACCTGTACTGGTGTTGGTGTTGGTATGGGTCTCCTACGCGATGGAGATATGAGACACCCATAGGTCGAACAGGTCTAATATCTGGAATCCAACGCTATTGTTGTAGAAGACGTTGCTCCCGTCCTTTAGCTTTGGCTGGTCACTATCCTTACGCTCCACGTACGG TCACCT SEQ IDNO:148 Middle LPZ-080AGGTGACCGTTGGGAAATGCAATACCTCTCGTCCAGGTATATTCAACTTGAGGGACAGAGCCAAGTGGGATGCATGGAATTCACTTAAAGATAAAACCCCCGAGTCTGCCATGGAAGATTACATCACTAAGGTTAAACAGCTACAAGAAGTAGCATGATGCCATTGATCTCCCTTTCCCTGCCTCTTCTATTATCAGTACGGTCACCT SEQ ID NO:149 Late LPZ-081AGGTGACCGTCAAGGCAAAGTGTCATGCCACTCATTGGAATTAGTTAATATAGCTAATTTGAGATATTACAGTCAACTGTGGGTATATGTATGTGAGATCAAGGTGCAGTTTAGATATTATCAGTGGTGCAGTTTAGATATTATCAGTGTTTGTGAATCTGCATACTGCTTTTGGTTGGTTCTAACTACGGTCACCT SEQ ID NO:150 Middle LPZ-082AGGTGACCGTAGACATATATCATGGAAAACCCAAGTAACATACAAACACAAAACACATGGAACTTCATAAAACCTCCACTCGTCATAAGCTTTATTGCTATGTTATTGTGGTGTTGCATCGTACTTAGTGGAGGTTATTGTTATGTTATGTGTTCTATTTTCCTCCCGAACGCCCTTCGGAATTGAGCTAACCGTGGTTAACAACATGTGGGCTTTTTTTCTCGACAGTATATATATAATAAATCTTTATTTTTTTAAAAACTAATGCTATTGCATTTATATACTGGAAAAAATGATTTTTCTGTATTATCGAAAATAATAATTTAGTTTCTTGATAATCACTTGGAATTAAGAAATTACAAACCCTAACAACATCAAGAAATTTTAAAACACATAAGCTAGAAATTTTAAAACACATAAGCGTGACAACAAGAAGATCAAATCTAATACTTGCTTGGGCCGGAGATTATGGATTCATGAAGCGATTTGACAGCGTCCATTGATCTTCCTCTCC CACGGTCACCT SEQID NO:151 Late LPZ-083GGGGGTAGGGGTGTTTATACTGAGCATACTTCGAAAGTGGTTCACCACCACCATGTGACTAATTGTTCCTGACTTTGGTAGACCTATAATAAATTCCATAGAAACCTCCGTCCATATTGATGCCGGAATGGGCAACGGTTGTAATGTGCCTGGTACTTTGACGGTCAC CT SEQ IDNO:152 Middle LPZ-084AGGTGACCGTTGGGAAATGCAATACCTCTCGTCCAGGTATATTCAACTTGAGGGACAGAGCCAAGTGGGATGCATGGAATTCACTTAAAGATAAAACCCCCGAGTCTGCCATGGAAGATTACATCACTAAGGTTAAACAGCTACAAGAAGTAGCATGATGCCTAGACAAATAGCTTTGCTCAACACATCCTGATAGTGTACACTAAATCGCACAACTTTACTACTACAAAGAAAGATCGTTGACACCTTGACAAATAGCTTGCTCAACACATCCCAACAATTTGGATTGCGAATACCGACTCCAATTTGTACTTGATCCATATGTCGTTGCGATGTACTAGTTCCTCTATACATATGTTTCTGCAAGAATCGGAGTTGGACCTCTTCTTCCCTGTTATCAGCACGGTCACT SEQ ID NO:153 Early LPZ-085AGGTGACCGTGGATAAGAGAACGCTTTGCCGACTCTCTGGGATGCCCTTCCCTCCATAGCCGTCGTGGGAGGACAGAGCTCCGGGAAATCCTCTGTGCTGGAGAGCATCGTTGGAAGGGATTTTTTACCGCGTGGATCAGGTATTGTTACTAGACGGCCGCTTGTCCTTCAACTTCACAAGACTGATGAAGGCAGCAGGGATTACGCCGAATCCTTCACCAACCCAGAAAGACATACACCGACTTTGCACTGGTAAGGAACGAAATTGCGGATGAGACTGATCGAATTACATGGCGTGCCAAGCANAGTCTCAAGTGTCCCAATTCACCTAATATTTATTCACCCAATGTTGTTAATTTGACTCTAATTGATCTCCTGGGTTGACAAAATTGCTATTGACGGTCACT SEQ ID NO:154 Middle LPZ-086AGGTGACCGTTGGGAAATGCAATACCTCTCGTCCAGGTATATTCAACTGAGGGACAGAGCCAAGTGGGATGCATGGAATTCACTTAAAGATAAAACCCCCGAGTCTGCCATGGAAGATTACATCACTAAGGTTAAACAGCTACAAGAAGTAGCATGATGCCATTGATCTCCCTTTCCCTGCCTCTTCTATTATCATTGATCTCTCTTTCCCTGCCTCTTCTATTATCAGTACGGTCACCT SEQ ID NO:155 All LPZ-089AGGTGACCGTACATACAAGTGCTCAGTACAATGTCATATACTACCAATACATTTGATTAGAATACGAGACTCGCTTTCATTCGGCATATCTGTCTCTGGATGATAAACATATAAAGCCTTGATCCATGAGTAAGGTAAGTTTGAAGCTACAAGTATTTTCTAAACGAAGTTCAAAATTACATAAGATTGTGGCTGGGGCGTGAGAAACGGCCTCAACAATGTCCTGTTCTGATCATGTATCATTTCAGTACCGATCATGCCTATCATACCCGCCTGGTGACGG TCACCT SEQ IDNO:156 Middle LPZ-090AGGTGACCGTACTGATAATAGAAGAGGCAGGGAAAGGGAGATCAATGGCATCATGCTACTTCTTGTAGCTGTTTAACCTTAGTGATGTAATCTTCCATGGCAGACTCGGGGGTTTTATCTTTAAGTGAATTGCCATGCATCCCACTTGGCTCTGTCCCTCAAGTTGAATATACCTGGACGAGAGGTATTGCATTTCCCAACGGTCACCT SEQ ID NO:157 Late LPZ-091AGGTGACCGTATAGTGTCAAGCTTTTCTGGATTGGATAATGGACGGCGGCTTGCGCATACATCTACACATTCTGTAACAAGTACACTCTACTGCAACAGCAGACCCAATTTCACCTCTTCAGTCAGCCAGAGATCTCGATGGATTTGGGTTGAGGAGGTTGGGGTTCGCCTGCTTCGGCACGGTCACCT SEQ ID NO:158 Early LPZ-092AGGTGACCGTGCTAAGTAATTATCATCTGTACCTGTGCTTGCTGCAGGAAGTAAACCAACCCGACTAGTCTTTTTAATAATACAGGGAGCCTTGCCACCAATTTCCTCTTGAAGCACCCATATTGGACGGGTTTGTGTCATCCTCTGTATTATCCTTTTTCATCCCAAGCAGGCTGTCTGTTTTTGTAGTAGAAGGATCACAACACAGATCAGGCCCTCCATAGTACAAAGAAGAACCGAGGAAAGTATCATTAACGTTCTGACTCCTGCCATGAAGGCTTCCACTATGACCTTGACCCTTTTGTGAATTACTGCCATTTAGACCTTGACTGGCTCTTGCAACCAAATGCCCCAGAATGGAACTTCTTTGTGCTCCAGTTCCATTGTGGTTAGTTGAATCCCTACCACGGTCACT SEQ ID NO:159 Late LPZ-093AGGTGACCGTGCAATATTGTATTCCAGGACCAAGTACTTAGGACAGAATCAGGTCACGAGTGGCTCCACTCCACAATACGATGTTCATCGTTTTAATCACAATACAAGTTTGTTAGTCCAAGTAAGTGCGCTGCTGCAGACAGTGGGGCACCCCCCGTGGGCTTTGACTGCCTGTCATACTGTTCCCTCCTTGCTCCTGCTCTTGCTCTCGCTGGGCTGTGGTGAGTTACTAACCTGGTTCGACCCACAAGGGCTTCTCACTAGGGCGTTAGGCTGCATGGATCTGCCAGATATTGTGGTTGCAAGGGACAGAGGCATGAGACACAGGCCTTTGCTTTGCAGAAACTGCATTGCTGACCCCATGTTCATCCATCAGTTTTGCTACCTCTCCTTCTGTTATGGACGGTCACCT SEQ ID NO:160 Late LPZ-094AGGTGACCGTATCCGCAGCAGCAACAGCAGTAGAGCCTGAAGCAGGGGACCTAATTACAGTCAAAAGTCCAGGGCTACCAATGCCTGCTAACAGCGCACTTACTGGACTAACAAACTTGTATTGTGATTAAGACGATGAACATCGTATGTGGAGTGGAAGCCACTCGTGACCTGATTCTGTCATAAGTACTTGGTCCTGGAATACAATATTGCACGGTCAC CT SEQ IDNO:161 Late LPZ-095AGGTGACCGTATCCGCAGCAGCAACAGCAGTAGAGCCTGAAGCAGGGGACCTAATTACAGTCAAAAGTCCAGGGCTACCAATGCCTGCTAACAGCGCACTTACTTGGAACTAACAAAATTTTTATTGTTAATTAAAAACGAATAACATCGTTTTTGTGGGAGTGGAACCACTCGTGAACTGAATCCTGTCCTAAGTTCTGGGTCCTGGGAATAACATATTGCACG GGTCACCTT SEQID NO:162 Middle LPZ-096AGGTGACCGTTACAGCTAGGGAAGACTTTAAAAGTTTGTAAAACTAAGCATAGCTCTAAACACTGAAGTTAAAAGACATGATTGGAATGTGCAAGTGGTTCAGTATCCAAATATTGAAGGTTGCAGAATATGGAGCTACTGTGCAAACGAGTAACTTTATCTATATTTTCACAAGATCATACAATGGGAAACGTTGAGATAACAACTGCATCGGTGAACCAGAATAGTTATAAAAGTTCTTGCAAGTAAAGGGATGAATAATTGCATGGTTGGAATTAAGAATGACCATGTAGAGCTGCTATACAGATTCTCCAAGGTTTTATATTTGAGGAGTGCGCGCTATTGATGTTGTGCAAAAATTTCAGAAATTAAGTTCTGCGGCATTTATCAAGGTGTTTGAGCCATTTAAATAGCAAGTTTTTGTTCTCCAAGTACTTTCAGGAAAGCAGATAGCTCTAGTTATAATGCTCCAGTGACAAACACATCTAGTTGGGGCAGTGAATGACGCTTTTGTCATTCTCTTTTGGTTTCAGGCACGGTCACCT SEQ ID NO:163 Early LPZ-099AGGTGACCGTGGACAAACTCTAGAACAGGCATAGCTTTCATGTTCAGTTGTTTTTAAAGAGCAGTCCTCGCAGCAGATCGTGCAGCTTCCTGCTTCACTTCCGTTGATTTTCCTGATCTGAAATACCCGTAAACTTGCTGAAGAACCCAAATACTTAATAGCGTCTCTAA ACAAAA SEQ IDNO:164 Late LPZ-100AGGTGACCGTGCCTGAAACCAAAAGAGAATGACAAAAGCGTCATTCACTGCCCCAACTAATGTGTTTGTCACTGGAGCATTATAACTAGAGCTATCTACAAGCCAAAACAGTGTTTGGGAGAGATTCCATAACGTCATTGCCTCTGCTACACATCATTCATTGGTTCCAATAATGAAGCCACGTGCTAAGGACATTGAGAGAATCTTATAAAACAAGAAATATAGTAAATTGGGAAATGCATTTTATCGTCTAACCTGCTTTCCTGAAAGTACTTGGAGAAACAAAAACTTGCTATTAAATGGCTCAAACAACCTTGATAAATGCCGCAGAACTTAATTTCTGAAATTTTTGCAAACATCAATAGCGCGCACTCTTCAAATATAAAACCTTGGAGAAGTCTGTATAGCAGCTCACATGGTCATTCTTAATTCACACCATGCAATTATTCATCCCTACTTGCAAGAACTTTATAACTATTCTGGTCACCGATGCAGTTGTTATCTCAACGTTTCCCATTGTATGATCTTTGAAAATATAGATAAAGTTACTCGTTTGCACAGTAGCTCCATATTCTGCAACCTTCAATTTGGATACTGAACCACTTGCACATTCCAATCATGTCTTTTAACTTCAGTGTTTAAGAGTATGCTTAGTTTTACAAACTTTTAAAGTCTTCCCTAGCTGTAACGGTCAC SEQ ID NO:165 Middle LPZ-101AGGTGACCGTAAAATACCATGAGAAATGCTTTCATCAGGCACCGCTGGTAGGTTTCTTAAGCTTTTCATTAGGCAAAAGAGGCTCCGTGAGTTGATCGTTAATTCTCTCCTTGAATGCCATATTGACCAGACACTCTGATTAGAAACTGGAATACAACTGCACATATAGTCATTCTATATGATTCATCCTTCTGCACTTCAGCATCCTGCGGCAACTCTTCATCCCGCCATACTGAGAAAAATATTTGACTCTTGATCATGTGTAGATGAATCTTCATGAATCTTCTCATCTTCATTCTTGTCTTTATATCTTTAGGAAGTGCATCTGGTAAAAGTATAAATGCATCTTCACGGGTGCTTCAGTTTTTGCATGCTCCCGGTCTTCTTGTTTAGCATGTGGATCTAGGAAATCACTAAATGTAGTTCTCTCAATTGGTCTGGTGGAAATTCTCCTCAATTCGAGAATTACGAATCATCATACCTGAGTAATATATGTTGCCCTGTACATGCATATGCTGGTTTTTGGCTCCACCATCTCCAAAGGGCTCAAAAACTATGCGACCCCTGGTTGCCGTAGTGGAAGGTTATACATTGCGTTCCCAGTAGCCACGGTCAC SEQ ID NO:166 MiddleLPZ-102 AGGTGACCGTGGAGGGGCTCCACTTATATGCATAGATGATGCTGCGAGGCTGTGTTCATCTGGTCCAATGGAGAAGGGGAAGACCAAGTGCCTATCCTGATTTTGGTGCCGCTTGTTCTGGTGTACAGAATATCAACCCAGGGTATGTACCATCACTCGTGAGACGTTCACATTTCCCCACTTCTTGGTGGAGCTGGTGGAAAGCCTGGAACTTCATCAATCTATCGTTGGTGTGAGGATGATCAGGCTCTGTACTTATATCCACATGTAGTGCAGCAGGTGGTGGAGATGTCTCTGATAAGTTGGGGGTTGATACTGGTTCGTATCATTTGCAGTGATGTCCCCCGCTGCCCTTAATTGCTATTGATCCATCATTAACTATAGGTTTTTACTCGCCCGGAATAAGACAATCTTTTGACACTTGTTGCTTGGGTCAC SEQ ID NO:167 EarlyLPZ-103 AGGTGACCGTGGCGCCTGACCTGTGCAGAATCCATTCTCATGGATACAATACTGTTAAGTTTGCTTTGCTTTGCTTGAAGGATCTGAATTGAAAAATTGTCCCCACAATTCTGTTTCGTTAAAAATGACCTCAATCACTCTCGACAGTTTCCAGATCTTGATTGGGAGCGTCCTCTCCTCTCTCAAGATGTTGTTGACCAAATTCAGGGCGACTTGTGGCCAGAAATCGTACATTCTGCCATCTACCTGTTATTGAGCTCCCCGATTTATATGCGCTTTTGAC GGTCAC SEQ IDNO:168 Middle LPZ-106AGGTGACCGTCAATACCATTAAACTGGGGATTCGTCTCAACAAGTCAACATGCTAACCTCACAGCTCCAATCAAACAACGTCCGTCGAAGGGCGCTCACACTCATCCAAATTACTTCCCTCTGCAAGACTCACAAAATCAGATTCTTCATGAATTGCTCAAACGAGGCTGTTATGGATGATGCAGCTGATTACTCAAGTGACAGCACTCTGAATCCCCGTCCCATATATAGCGACGCGGCGTTTCAGCCGTGACTGGTCGCAACAGCCTCAGTGGGACACAAAAGGCCAGAAGCCCCCCAAGGTTCTCACGGTCAG SEQ ID NO:169 E,L LPZ-107AGGTGACCGTGTCGATGTTGTTAGATGTGATTAGGGTTTTATTTCTTGATACAGATGCACTGTTTCTCTGTTTATTCTTTTATTTCTTCAATGTATGTTGTCAAATTATACTTAGTCA1GATCTCCTTTTATCGTTCGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTTTAACAATTAAAAGGGGAAATTAGGCCATATCAGCTTGTCGTATGGACCCACATG ACTGTAGGTCACSEQ ID NO:170 Middle LPZ-108AGGTGACCGTATGCAGAGTCAAGGTTTAGTTCCTTCAGAGCCTGCCCGAGTAGCACTGAGGCAGCTCAAGCCATTTCACGTAGGAAGCCCACAACAAAATAGAAATCAGAGTGAGTCTTTGATCGAGTAACCCATAAGTTCTTAGCTCCCGTTCCATCTTAACATAAGCATTTTTCTTCGTCTTCTCGCAGCCGT SEQ ID NO:171 Late LPZ-109ATTGCAGAGGACTTAGAGAGGGAAAACCGTTCCGATCTGGTGAAGCAATTGGATGAGCGCTCTGGAATTGATCCCGTTTCTGATGATATCGTACGGCTAAGCTCAGCTCTTCAGGCATTGGCAGACAATACGATTCTTCAAATGAGATGACAGATTTTAAGAAACTTATAGGATGACATATTTCCTAGCTTGAAGCGGATCCCCCTACGGTCAC SEQ ID NO:172 AllLPZ-110 AGGTGACCGTCCGATAAAGGATGAGAATATAGGTAGATCAACCCAAAAACACTCTCAGAAAACGATTAAAGCCTAACCCCAAGATCGTTGAGTAAATTTAACCCGGTAACCTCCACATAAAATATACTTAGCAACAATAAACTCAACAACTAAACTATCCCTTTAAAATTAAATTATCCTATTTATTTAAAAAAACAAATCCTTTATATACTAAGGTCCCCTGCACATCTATTACTAAGGTAAAGGAAGGGAATTATATGCTATCATTGTAAACTTTGACTTCCGTATTTATGATCAGACCATGAGTTTGATAATTAATTTTACGCTCTTTACTCCCCATTCAAGGCACGTGCCTGGTGATATATGAACGCCAAATTATT SEQ ID NO:173 Late LPZ-111AGGTGACCGTAGAATACAATCTATGTATCTTAAATGCTAACAAAGAGAATTTGTTGTCTAGCTTGTAAATATACAAAAGAAACTCTCACAAGGAGTGAGAAGCACTAAGGCCCTTGGAAAGAATACGTTTCTATTCAGCGGAGTGTATTTTGAGCTACGGCTTGGCACAACTCATCCTATAAAACAAGACTCTGTGAGAGGGCAGAGACCTTGATCCTGGGCGTGGCAAGCCGGGTGCCTATTGCGGTAAAATCGAGAAGGGGGACCCTGGAAAAGAGAGGCTGAAATTTGTTTCATTCTGCAACTGAAACCTAACCGGAGGCCGAATCTGATCATTTCTAAGACCTTTGGGGTCCTGGGCATCCCATTAAAAGAACGCTGCTAACTCTCCCCTCCACAAAGGGCCAATGCGCTCAGGTCGGGCTTCTCATCTTCACATTTCTTGCCGAAATCTATCTGAATTTGTTGTATTGAATAACACTGCCTCCTACACGGTCAC SEQ ID NO:174 LateLPZ-112 AGGTGACCGTGGGCGCCGTGGCTCAAAAGGCCCTCGCAGACGCCCGCTCCATCAAGCTCATGGGCCCCCTCCACCCTCGGGGGGCAAGCCGGGAACGTTGCTGTCAGACGAGGCGAGGACCTGGAACTGCCGTTGAAGGAACGGTTCTATATTCAGCCCCTCTCGGCGGACCAGGCGCTGCGAGAGCCAAGGAATCCGCGGAAGCAAATCCTGGAGGTGAAAAAGCTGATAGATAAAAGGCGTGGCCGTACGTCCAGAACGACCTCCGCTCCAAGGCTTCTTACCTTCGCTACGACTCAACACCGTTATCTCCTCAAAGCCCAAGGAACAGAAAAAACCCCTCAAAACCTCACCCCAAAGCTTTTTTGACACCCTTGACAAACCTGGACTACGCTGCAAGGAGCCAAGGATACCCCAAGGGCAGAAAAAATACTTTGCAGAAGCTGGTGAACCGCCCTTAATGATGTTCATTCCAAGCTTGGTTAAGCTGTATTGCACTCATTGTTAACCACACTTAACGCCAATCCAATCTATGCTGTGTTGCATCTCCACTTCTTAGTTAATAACGTCTGTGTCCCAAACTCTGTGCCACACACGGTCAC SEQ ID NO:175 AllLPZ-114 AGGTGACCGTACAATACAAATAGGTAGTTTATCACATTGTAGCTTATAGAATGTACAATTGAAATCAAATAAATTCAACCAAACTCAAATAATATGATCATGTGCTCCTCACCTTCTCAGCAAACTCGTAGAGCAGAAAAAAGGATTATGTTAAATCACAGTTCACACATTAGGGTAAATCCCACTAAATGACCTCTCTTCATTATCCAAGTATCTGACACCAACATATTTCAAACAAATAGTGCAAAAAGGAATGGTGAAGTAAAATAGTCAAAACTAAAAAATAAGCTTAAAATTTCTCACATGTTTGAATATGTGCACCACAAATTTTGTTAGTGTCATCAAAATGCATGTAATCAACTTGCCGTGTATATAATTTCACACAATATCCGTAAAATTTTGCAATTCCTTATGAGCATTTCATGTCTAGAGATTGCAATGACTTGGCTACAAACATGTTTCTCTACACAAGATCACAATATTTAGTCAGGACACGAATTGCAATGGGGATTCTCACAAGCATCACAAGTCATCTCCCATGTACTAATAAAAAATTGTTTAAAT SEQ ID NO:176 MiddleLPZ-115 AGGTGACCGTATAGTGCATATTCAGATTGCAATTACAGACGTATTAGAACCAGATTTTCGCTTCGATACAGCTCATCGAGAGCAACAGAGATCCAGATCAAAAACCAGACACAGTTTAAGAACATCGAAATACCAAGCCCAGGGACAGTTACCAGCATATAGCTCTACCACCAACAGATTATTACAGAACCAAAACATAAGACCACTTGCAGACAAAAATAAACCCTAACGCAGAACGTGGCAACTATCTCCTCCAGCTACCACCATCGGAACCACCACCACCATAGCGAGAACCCCACCACCACCATAGCCGCCACCGCCACCACCATAACCACCACCACCACCACCACTGTACCGCCACTACCGCCATAACCACGGTCAC SEQ ID NO:177 E,L LPZ-116AGGTGACCGTCCTTGGAGATACCAGCTTCAAAACCTCCAGTGGTGGAGTCGATGACAATACTGCACAGTCAGCCTGAGATGTTCCAGTAATCATGTTCTTGATAAAATCACGATGGCCGGGGCATCAATCACAGTGCAGTAGTATTTAGTTGTCTCAAACTTCCAGAGTGCAATATCATTGTGATACCACGGTCAC SEQ ID NO:178 E,M LPZ-117AGGTGACCGTATAGTAGGAACTTTAGGTGCTTTTGGTGGCACTCTCCAATTTTCATGTCCTTACATACCCCACTACGGAGAAGGGTAGCCCAAGATTTGAACCCAAGACTTCCGGTTCGTGAGACTTCATTTCCACGGTCAC SEQ ID NO:179 All LPZ-118AGGTGACCGTAAGATCAAGAGCACAGAAAGCAGCCATAGCCCCGCCCATTGAATGCCCATAACAATAATCTGTAACCCATCTCTCTGTTTCTGAGCTTTCTGAACTGCTTCTACAACAGTGGTCGTAAGGTTGTGTTGTGATAAGCAGAGTAAAATCCATAATGTACCATTGCACCAGCATATTAGGATAGTTGAGATCAAGTGTCTTACAGAATAAATCCTCCACCCAATTCTGTAGCTCCTTTCTTGAGTACCCCTGAATGCAATTACAATTGCATTGATATCTTCTGCCACACCACAAAAGCCTGAAGGCAGTGTTGTACATCAACTATAAGCTCACCACCTGAAAACCCCAGTCAAACCATTGCACCTAGAACAAGTCCAAGACATTAGAGCACTCAAATCATCCATATAGACCGCAGAAGCATATTGCACAAGTATCTCAGCAAGTGTTCGATTATAGACATGGCCA1GGTCAC SEQ ID NO:180 Middle LPZ-119AGGTGACCGTGGGAGGGGAGATTTTTGATTTATATTTCCAATATAAAAGAAAATCTANGTTGTAAGGACATGGCAAGAGCTCTTATTTCCGGGGTTTTAGCCGTGGCCCGGAGCGGATGAAAGCAAATGTAAGTCACTCCGTGCTTCTCGGCATTTGGACGCTTCTACTCTACCGCACTACAGACGGGATTGAACCTCGCATCTCTGAGTGTTTGGTCGTTTACATGGCGGACTTGTTCCGCACCTCTGCGGACGTCAAATGCCGCGACGATAATCCCTTTGAGAACAGCGATACGGCAGAAAGATCGCCGTTGACGAAGCGAGAAAACTATTGAGACTTGCAGATGTGGAGCTGAAGAAGAGCTTGAGTCGACGGTCAC SEQ ID NO:181 MiddleLPZ-120 AGGTGACCGTCCGTTCGGGGTGTATTTGTCGAACACGTAGGATGGTGCTACGTTGAAACCACCGTTACCTTCTTCGATATGTTATAGTTCGAGTTCATACGGAGGGAATACCGTTTGTAGTGTTATTCAGCACAACCCCGTCCTGATTAAACACCCCCGCAACCAAGGACGTATTCGACGTTCGGTATTGTTTGACACACTCAAGTTATAACCCTGAATAGGCGCTACCCGAAGTAAGCATTGTACCAGTCGTTATTTTTGCCTTCGTATGCGAAGGATTTTGAAATATATCCGGACAGGCTGCAACCGATCTTCATAAAACTCTTTCTTAAACTGAGCAAACTGAACAGCATTAGCATTTTGACCCGACCTTCATCGGCACCTGCTGCACACCCGCATACGTATTAAAGCTATGTTCGTCTGGCCAGGTTTGCCTTTTTTGGTTGTAATCAGGACAACGCCGTTAGCCGCCCGCGATCCGTAGAGCGACGTAGAAAGCCGCAT CTTTCAGCACGGTCACSEQ ID NO:182 Late LPZ-122AGGTGACCGTGAAATATGTGGGAGATGATATGTGGTTTCCTGAATATTCACCTCTTGTGTAGAAAAGTGAGATCCTTAAGATGTTTTTGCTAATAAGACTCTTAGGAATGTTGGACCCCTTTCAGAATGCCATTTGAATAGATTCAAGGTGGTAGCTGTTGCCTGGGGCTGTTTTAGGGTTTTAGGCCATGCTCTGTAATTCATTGAGTCAAAATTGGATTAACTGGTGTCTTTTACCTCATAATAGCTACTGCAGTATTTGTCGATATAGCTTCCCTATTTATTGACTCTCCTTAGGTACGGTCAC SEQ ID NO:183 Late LPZ-124AGGTGACCGTCCGTCGGGGTGTATTGTCGAACACGTAGGATGGTGCTACGTTGAAACCACCGTTACCTTCTTCGATATGTTATAGTTCGAGTTCATACGGAGGGAATACCGTTTGTAGTGTTATTCAGCACAACCCCGTCCTGATTAAACACCCCCGCAACCAAGGACGTATTCGACGTTCGGTATTGTTTGACACACTCAAGTATAACTCTGAATAGGCGCTACCCGAAGTAAGCATTGTACCAAGTCGTTATTTTTGCCTTCGTACTGCGAAGGATTTTGAAATATATCCGCACAGGCTGCAACTGATCTTCGTAAAACTCTTTCTTAAACTGAGCAAACTGAACAGCATCAGCATTTTGACCCGACCTTTCATCGGCACCTGCTGCACACCCGCATACGTATTAAAGCAATGTTCGTCTGGCCAGGTTTGCCTTTTTTGGTTGTAACAGGACAACGCCGTTAGCCGCCGCGATCCGTAGAGCGACGTAGAAGCCGCATCTT TCAGCACGGTCACSEQ ID NO:184 Middle LPZ-126AGGTGACCGTCGTCAGAAAAAACGTGATTTCCGCAAACTTTGGATCACTCGTATCAATGGGCAGCTCGTTTGAACGGACTTTCATACTCACAATTGATGCATGGTTTGAAGGGCTGAATCGAAGTGAACCGTAAAATGTTGGCTGACTTGGCTGTTAACGATGCAGCAGCTTTCAAACTCTTGCAGACGCAGCTAAAGCTAAGCTTGGGTAAATAATTAAAAAAAGAACCGAGGTTTCCTTGGTTCTTTTTTATAACTTTTAATGAAAAGTATGAAGAGAGAAACAGCCTGTCTTCTACTTATAGTATAAGATAAAAGCTTGTTACTGATAAGACAGCTTTCATGGTAAAGCAGTTAAAAATAGGGATTTGCGATATAATAGAAAAAACAGACGTTTATGTAAATAAAAAACAGTAGAATGGAGAAATATGTCAGAGAATCGTTTGGCTTGGGATCAGTATTTTGCGGCCAGGCTCTCTTAATCGCTAATCGCTCAACCTGTAAGCGAGCCAAAGGTGGCTCCGTATTGTCAAGGATAATAAGGGTTATTTCAACTGGGTACAATGGCTCAGTTTCAGGGACTGGAGACTGTATTGACCAAGGAGTGCCTGGTCATTGA CGGTCAC SEQ IDNO:185 Late LPZ-127AGGTGACCGTGGCGGAGGTTAGGGAAGTTTGACTTCTCATTTTCTCACGCACTCCTCTCCTCGTAACCTCGGTCGAGTCGATGGCGGCTTTTTAGTCGAGTGTGCTAACGCCCCTCCGGCCTCAAAATTTCCAGCTACTCGTATTTGATCAATGCTGAAATCGCGTAATTACGTAGTAATAAAGCGTAATGAATTCTATAATGAAGCATGTTTCTCTATAGTTCATGTGCCGAGAGGAATAATGAAAATGAGGCCTTATATATTATCTGGGGCTCAAGGAGATGTTATCTTTTCCTCCTTGGTTAGAGACCGTCAACCTTCACTTGATTGGATAAAGCTTCATTTTGTTAAAACCTCCAAGCCAGTAGATACATACGGTAGGCACGTATTATGGTAGAGACATACGGTCAC SEQ ID NO:186 Late LPZ-128AGGTGACCGTCCTGTTGCCTTAACCGCGAATCCAAATCGACTTGGGCTGCTTCCTTTCGTGCAGATATTTCTGGTTTGGACTCTAGTTCTTGCTCCTGGAAATCATGCTTGAGTGCTGGGTAGCTGCCTCCAAGTTTGGTTGACAGGCCCATTCCTTACAGCTTCTCTCTTCCGCTTATGACAGAGTAATGACAGGAATTCAACCTGACGGATCCGTCTAGCTCTCACAAGGTTGGGACCCTGTCTTCGAGAGGGTTATTTCTTGAGACTGTTGACTATATTTGGATGAGCCCTCAGCTCTGTGTACTATTGTTCATGTACTGGATACTTTGTAAATGATTTTATTCTGGTTTTACCCCGGGGGGGGCATTTTGACTCCTGGGTTTAATACGGTC AC SEQ IDNO:187 Late LPZ-131AGGTGACCGTGGAACATGATGATTAGTTCTTCTGTGGGCCAGGATGATTAGTCTCTGTGTGACTGTGGGCCAGGATGATTAGTTCTCCTGTGACGACTGTTGGATAGGATGATTCGTCTCCTGTGGACAGGATGATTAGTTCTCCTGTCGAGGCACCCTACCCATGCAATTTGGGATCATGGGAAGTACCTCTCATCTGATCAATGAGTAGGGAAATGGGGTAGGGACCATTAGAGTACTATCGATGGACACATCGTTGTATCTACCGTCCTATGCTAGGACGACCTCCATTGTTTGGGATTAGTGAGAGTGGTATGACACTCTGAGACTGACTTTGGGTCAGTGGAGGATGTATGATACATCCTCGATCATTTCTTCTTCTTCATAGTTCGAGCAGAGCAGAGCACAACAGGCCAAGTAGTGCAGGGTAGTGCATTTGATGGCTGGGATAGTAGCGACGGTCAC SEQ ID NO:188 Middle LPZ-133AGGTGACCGTAAATAAGATGACCCACATGGAGTTTGGCCCTAGTTTCCAATTTTTAACAC1CGCTCTCAACTAGGGAGAACTCCATTCGCTGATCCATTTGTCCGACTATACTATCTCTGCATCAGTGCCCTACACTACTCTGCACTGCTCTGCTCTACTAAACCATGAAGAAGAAGAATGACCGAGAATGTCTCATGCCATTCTCTATTGACCTGAAGTTAGTCCTATATGAAGAGA1TGTGTCATATCACTCTTATTGACCCAAAGTCAGTTTTATTGATCCCAGATCAATATCACAGAGAGTGTCTCAAACCACTCATACTGATCCCAGATCAGTTTCATTGATCCCATATCAAGGAGATCATCCTAGAATAGGGAGTACAGTAGATACAATGATGCATCCATCAATAGTACTTCTATGGTCCCTAACCCCATTTCCCTGCTCATTGATCAGATGAGAGGTACTTCCGATGAGCCCACACTGCATGGGTAGGATGCCTCGACATGAGAAATAATCATCCTATCCACAGGAGACGAATCCTCCTGTCCCACGGTCAC SEQ ID NO:189 MiddleLPZ-136 CTAGGGAAGACTTTAAAAGTTTGTAAAACTAAGCATAGCTCTAAACACTGAAGTTAAAGACATGATTGGAATGTGCAAGTGGTTCAGTATCCAAATATTGAAGGTTGCAGAATATGGGCTACTGTGCAAACGAGTAACTTTATCTATATTTTCACAAGATCATACAATGGGAAACGTGAGATAACAACTGCATCGGTGAACCAGAATAGTTATAAAAGTTCTTGCAAGTAAAGGGTGAATAATTGCATGGTGTGAATTAAGAATGACCATGTAGAGCTGCTATACAGACTTCTCAAGGTTTTATATTTGAGGAGTGCGCGCTATTGATGTTGTGCAAAAATTTCAGAAATTAATTCTGCGGCATTTATCAAGGTTGTTTGAGCCATTTAAATAGCAGTTTTTGTTTCTCCAGTACTTTCAGGAAAGCAGGTTAGACGATAAAATGCATCTTCCCAATTTACTATATTTCTGTTTTAAAAGATTCTCTCAATGTCCTTAGCACGTGGCTTTCATTATTGGGACCAATGAAGATGTGTAGCAGAGGCATTACGTTATGGAATCTCTCACCAAGAACACTGTTTTGGGCTTTAGATAGCTCCTAGTTATAAATGCTCCAGTGACAAACACATCCTAAGTTTGGGGCAATTAATGACGCCTTTTGGTCATTCTCCTTTGGGTTT CAGGCACGGTCACSEQ ID NO:190 Late LPZ-137TCCCTTTAGTGAGGGTTAATAGATCTATAGTGTCACCTAAATCGCGGCCGCTCTAGAACAGTGGATCCGCAAGCAGGATAGACGGCATATGCATTGGATGCTGAGAATTCGATATCAACTTATCGATACCGTCGACCTCGAGGG SEQ ID NO:191 Middle LPZ-138GGTGCGATCCTAAACATGCAAGCTTTGAGTTTGTAACTTTGTAGAAGTGGACATTTCTAAGTTGGATGTACAAATCTACTGTTGGTTGTATTGTCATCCCATAAACAACTGTTTGATGAGATGTTTTTTTAAAAACCACATCATAATATTTTTAGGCCTTGTAAAAAAAAAA AAAAAAAAAAAAASEQ ID NO:192 Late LPZ-140ATTCCAAACTTTTCTTCAAGATGTACACCAACATCATTGTCCCCAACTAGTAGACTGACTTTTCACCAGGTCCAAAGAGAGGGGTGGTGGAAGCAGATTTCAGGCTTTCGAATAAGTATCAATGATATAAGCATCATCCCCTTGCCAATTGTTCTGGATCGCAC SEQ ID NO:193 AllLPZ-141 GGTGCGATCCCATCAGGGGTTGTGTTTCTAAGAATCACTTCCATGTTTCAAATTCAGCACTTGATCTTGTACATACCCAATTTGTTGCCTGCTACTAGCTAGTATTGTCTTTCAGTTTGAACCATTTTTTTGAGTAAATCGTGTTTAGTCTTTGGCAAAAAAAAAAA SEQ ID NO:194Middle LPZ-143 GGTGCGATCCGCATTAGAGAAGCATACAGGAAAAAGAAGTACCTGCCTCTTGATTTGCGCCCAAGAAGACTCGTGCTATCAGGCGACGCCTTACCAAGCATCAGGCATCATTGAAGACGAGAGACAGTTAAAAGAAAGAGATGTATTTTCCAATGAGAAAGTATGCAGTCAAGGTGTAAGCCACAGGATTTGAGCTTTCATGCAATTTTTTTGTTACTTGCGGGATGATATTGCCTATATATTTCCGTCCACGTTTTTGGCAAATTCCGATTTGCATCAGAATTCAAGTTATGATAGTGTTCTTCGCTTTTGAGCAGTTGATATTGTTTATCTTTTATTCTCTGAATTGCAACATATTCTAATGCAATGAGTGGATTATATATTGTGGTATTTCCATGTTGAACTCATATAAATGAGCGTAATTGAGTGGTAGCGCTAGGATATTTACACTTGGCAAAAAAAAAAA SEQ ID NO:195 Middle LPZ-144GGTGCGATCCGTATAGGTAGTTTGGATGATGAACGGGCAAAGAAGGCAAAGGAGTACAGGATGGATCCTGTAATTCCTGTTTCAGAAAACAGAAAATCTGCAATATAAGGATGGCTAACTTTTCAGCTATGAAAATATATGGTGCAGTGGCACTCATATCAGTTGCAGGTTGTCAAATAACTTTTGTGAATAGGAAAGTTGTCCTCTTTTAGAGTGCAGAAATCCTGCAATATAAGATGGCTAAGTTTTTCAGCTATATGAAAATATATGGTGCAGCAAAAA AAAAATA SEQ IDNO:196 Late LPZ-145GGTGCGATCCCATATACAATTACATATATTTTCAACAATTCTTTTGTTGTTATGAAAATCTATTGAAATAAATTGAAATAGTTTGCATCATTTATTTATCGGAATTCGTATTTATATATTAAATTTCTGATGTCTCAAATCCTTCGTTACTGTAACGATATCATTAATATAATGTGTCTGCAAGTTTATTGGGCAAAACAAAATTTATTTTTCGGTCACATCATAAGTTTATTTTTGGTCACATCATATGCACCATCACATTAAGCATAAGCATATACAGTAGCGTAAAAATACAATTATTGTTGTTGACTAGGATCGCAC SEQ ID NO:197 Late LPZ-146GGTGCGATCCTAGTCAACAACAATAATATGTATTTTTACGCTACTGTATATGCTTATGCTAATGTGATGGTGCATATGATGTGACCAAAAAATAAACTTATGATGTGACCGAAAAATAATTTTGTTTTGTCCAATTAGACTTGCTGTATATGTCTGGAGTCCTACCCTTGAATTGACTTGTTTCCC SEQ ID NO:198 Late LPZ-147GGTGCGATCCCATATACAATTACTTATATTTTCAACAATTCTTTTGTTGTTATGAAAATCTATTGAAATAAATTGAAATAGTTTGCATCATTTATTTATCGGAATTCGTATTTATATATTAAATTTCTGATGTCTCAAATCCTTC SEQ ID NO:199 Late LPZ-148CCACTGCACCATATATTTTCATATAGCTGAAAAACTTAGCCATCCTTATATTGCAGATTTCTGTTTTCTGAAACAGGAATTACAGGATCCATCACTGTACTCCTTTGCCTTCTTTGCCGTTCATCATCCAAACTACCTATACGGATCGCAC SEQ ID NO:200 All LPZ-149AGAGCCTTCTTGCAGACAATCCGTGAAAACATGGCTATACAATAAAAATTCCCAGTTTGAATTCTAAAGAAAACTGTTCAATATTTGAAGGCCTCTGATATCACAGAGACTGATATTAAATGGAAATTCATACAAATGAGGAGAGCATGTAGCAACACTAGAAGCTTTGGCATAAAGCACCAGATAAATTCATAAGAACTAAATCCATAAGAAGGATCTCTCGTTCACCAGTCACAATCACACTCGGATCGCAC SEQ ID NO:201 Middle LPZ-150GGTGCGATCCCTGGCCCTGATAACTTTGGTTGCAATGGAAAATGCAGTACTAGGTGCGAAATGCTAAAGCCCGCCCGGAGCGGTGCATGAAGTACTGCAATATTTGTTGTAGTAAATGGCTGGTTGTGTTCCCAGTGGTCACTATGGCAACAAGGACGAGTGCCCCTGCTACAGAGAATGAAGTCCGCAGCCGGCAAGCCCAAGTGTCCCTGATCTTAGCACTTCAGTCCAGTCGCCACTTCTTTTATTCTCTTTTTTTATAAAAGTGACGAGGCCGTTCTTGTGCTTGGTGCCATATGTAGAGCGGTGGCTACTTCTCCTGTGTTAGGAAATGTTGCAGTACTAATAATAGAACTTCTT SEQ ID NO:202 Middle LPZ-151GGTGCGATCCAATAAAGATATACTTTGCAACAATAATCAAAATATCATATGCAAAGTTTAAGATCAAAATAGAATGCAACAAAAAAATGGTTGTAACATAGGAACCAACAATGTTGCATTCAAGTAAGACTCTTTGCAAAAAAAAAAAATAAAAAAAAAAA SEQ ID NO:203 MiddleLPZ-152 GGTGCGATCCACAAGTAAGATAATTGAGTATATATTCAAGATGCAAATATTTCATTAGGACCACTCATAAAGTTATCAATGATTCACAAAGAGACCTCCTGACCTCTCTCAAAAGTGGTGGCAACACAAGACTAGTGTAGTTTTTACTATACCTCAATGAAACTACCATCCTAACTGATGCCATAATCTTCTGTTATATATTACCAAAATTTATGAGATGATTGATCCATAAACACTCCAGAACACATAGTCATCCAAAGGAACCTTTGCTTGAATATGGACCCCCTTAATTCAGGTACTTGCTACTCCAATAAATTGCTTAATCTCTCCACCGATAACCAC AGTTTGGATCGCCSEQ ID NO:204 Early LPZ-153GGTGCGATCCAGGACATGAGGCCGAGTTTGCCATTGTGATATGATTGAGGAAGTCCAGTCTCAAAATTAGGTTTATCTTGATGTTTGACAAGAAATATAGAAGGGCATGATGAATCAAGAACCTTTTCCAAATCTGTTACTGCAACCAATCCAATGACATAATAACGCCAATGGTGGTTCCTGTGATGACATAATAAATTGGATTAAATTAATAACATCCCTAATGCCATGTGGTTAGCTGCATCATCACCGTATCCATCGAGTGTTCAATTTTTGGGATGT TGTATCAAAAAAASEQ ID NO:205 Early LPZ-154AAATATTTTTCAATACAACGCCATGTGACATTTTTGTGCTTCTTGTTTTTGATACATACTTCCAAAAACTGAACACTCGATGGATACGGTGATGATGCAGCTACAGCCATTGCATTACGATGTTACTAAATTAAATCAATTTATTATGTCATCACACGAACCCAAACAATAGCGCTATATGTCATTAGAATGGTTGCAGTTACAGATCTGGAAACAGATCAATGAATCATCATGCCCTCTATATCTCTTGTCAAACATCAAGATAAACCTAATTTTGAGGACTGGACTTCCTCAACATATCACAATGGCAAACTCGGCCTCATGTCCTGGATCGCAC SEQ ID NO:206 MiddleLPZ-155 GGTGCGATCCGTATAGGTAGTTTGGATGATGAACGGGCAAAGAAGGCAAAGGAGTACAGGATGGATCCTGTAATTCCTGTTTCAGAAAACAGAAAATCTGCAATATAAGGATGGCTAACTTTTCAGCTATGAAAATATATGGTGCAGTGGCACTCATATCAGTTGCAGGTTGTGAAATAACTTTTGTGAATAGGAAAGTTTTCCTGTTTTAGAATGCAGAAATCCTGCAATATAAGATGGCTAAGTTTTCAGCTATATGAAAATATATGGTGCAGCAGAGTTGTCAATATAAACTTGTGAATAGGGAAGTTTTGGCAAAAAAAAAAAAAAGAAAAAAA AAAA SEQ IDNO:207 Late LPZ-157GGTGCGATCCTCGTTGTGAAGACGTAGTGATGGAAAGGTCATGTTTGTAGGAGACATAATTATAGGAGTTTCTTTATTATAATAACCAAGAAGTCCGATCCTGGGGGCGTTGAGTATATAGTCAGTCTTTGGTAATTTGGTGTGGTGCTGTTTGACCTGCCTTTCCTTTGGAGCAATGATCCTTGAGGATGGAAGAGGTTATGTTGAGGCTCAAGAGATGATTGTTTGAGTTGTGGAAAGCAAAAGGTTTCCAGATGTAGTCAGATAGTAACTTCTATGCTTTTAATAAAATTTAGTCTGTGGGGCATGCCCCTTTTTGCTGGCAAAAAAAAAAA&GAA AAAAAAAAAA SEQID NO:208 Late LPZ-158GGTGCGATCCGTATAGGTAGTTTGGATGATGAACGGGCAAAGAAGGCAAAGGAGTACAGTGATGGATCCTGTAATTCCTGTTTCAGAAAACAGAAAATCTGCAATATAAGGATGGCTAAGCTTTTCAGCTATGAAATATATGGTGCAGTGGCACTCATATCAGTTGCAGAGTTGTGAATATAACTTTTGTGAATAGGAAAGTTTTCCTGTTTTAGAATGCAGAAATCCTGCAATATAAGGATGGCTAAGTTTTTCAGCTATATGAAAATATATGGTGCAGCAGAGTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAA SEQ ID NO:209 Middle LPZ-162GGTGCGATCCCAGGAGAATATTAGTTTCATGTGTTGCTATCATTTTCTTCAATATGCAGGGCAACCATTTGAATGAAACTATTCCTTTCGAATTTCAAAAACTTAATAGGCTAACTTATCTATCTGGAGCCGATTTTCATTGACGAGTAACCTGTAAGCTGGCCAGCAAAAGCCAACAGATGTTCAGCTTGTTGGAACCAGTTGAAGATTGTAATAGAGATGGTGAATAATCGCGGACGGCTCGGCCAATGGAATATTTGTTGCATCATCATCAAGGGGGTATGAATTCCAAAGAACTTGTTGATTGAAATTCCCAAGCAAAATTCTGTGAAATGAAAAATTTATTGAGACCATTGGGCAAAAAAAAAAAAAAATAAAAAAAAAAAAAA SEQ ID NO:210 AllLPZ-165 GGTGCGATCCGACTGTGATATGTGACTGGTGAACGAGAGATCCTTCTTATGAATTAATCTGGTATCTTTATGCGAAAGCTTCTAGGGTTGCTACATGCTTCCATTCTAATATCAGTCTCTGTGATATCAGAGGCCTTCAAATATTGAACAGTTTTCTTTAGAATTCCAAACTGGGAATTTTTATTGTATAGCCATGTTTTCACGGATTGTCTGCAAGAAGGCTCTTTGGCAAAAAAAAAAAA SEQ ID NO:211 M,L LPZ-166TTTTTTTATTTTTTTTTTTTCCAACGAGATCACTGTCATTGTTCAATAACTATATGCCAAAGAGCCTTCTTGCAGACAATCCGTGAAAACATGGCTATACAATAAAAATTCCCAGTTTGGAATTCTAAAGAAAACTGTTCAATATTTGAAGGCCTCTGATATCCCAGAGACTGATATTAGAATGGAAATTCATACAAATGAGGAGAGCATGTAGCAACACTAGAAGCTTTGGCATAAAGACACCAGATAAATTCATAAGAACTAAATCCATAAGAAGGATCTCTCGTTCACCAGTCACATATCATACTCGGATCGCACC SEQ ID NO:212 Middle LPZ-167GGTGCGATCCGACTGTGATATGTGGCTGGTGAACGAGAGATCCTTCTTATGAATAATCTGGTATCTTTATGCGAAAGCTTTTAGGGTTGCTACATGCTCTCCTCTTTTGTATGAATTTCCATTCTAATATCAGTCTCTGTGATATCAGAGGCCTTCAAATATTGAACAGTTTTATTTAGAATCCAAACTGGGAATTTTATTGTATAGCAATGTTTTCACGGATTGTCTGCAAGAAGGCTCTTTGGAAAAAAAAAAAATAAAAAAAAAAAA SEQ ID NO:213 Middle LPZ-169TCCCAAAGGCAATTATACATGGATCGCACC SEQ ID NO:214 All LPZ-170GGTGCGATCCCCACTGCAGAAAGATGAGCCAGTACCCTGAAATTTTGCTGTTGTCCATGCCTGGGTCACGGAGGAAAGAACGGCACGGTGCAATATGATTTTGCTACATACAAGTTCCAAGAGTGGATGCAGACAGTGCTGGCCATGGCTGATTATTTGCAGGTGACTAATGCTCTTTTGGTTATCCTTACCATCATCATCTTCCTGCCATTCTTTTGTACCTCGGTATGGAGACGAACACCCACTTTTCAAAGTTTGCAGAGGAAGCATGTATTCATAACAGGAGGATCAAGCGGCATTGGCCTTGAGATTGCCAAAGAGGCTCTTCACAGGGTTCTTACGTGACACTGGCGTCAAGAAATCTTTCTAAACTTCGTAGGGCTGTTTGAAGAAATCATCCAAGAAGTGGAGTGCGACGGAGACAAGATTAATATCAAGGTAATATACCCTGCAAAATGTTGTCTGGAATACAATCCAAAACCAATTTAGCAATTAACCCATTGGCA AAAAAAAAAAASEQ ID NO:215 All LPZ-171GGTGCGATCCAAGTGCGGTATTCTTCCTTTGGCAGTTCTCTGAACTGTTGAGAGAATTTGAGTAGGATAACGACATAATTACTATGCTCACAAGCCCAGACAACACGAATAGACTCCCTTCCGTGCGTCGCCTTCCAGAGGACGCAGCAGCTAAAATCTCGGCCTGACTCACCACATATATATTTAATAGCTTGTATATGCCATATGAACTGTTAGCATGATCTCCCTCTAACTGCGAATTGTGTTGCTGTAAACTAATCCCAAAGGATGTTTACTCTGTTGCTTTTCCAACTGCTGATGGATTTCGCTCATACAATGACCCGAGAGCACCATAAACCACCCAGCGTTGTGGCCTATGACCCATAGCTTTTTGTTCGCACAGCAATTGAAGACCGGCTACAGGAGATGACTAATGCACTTCCGAGAAGGTTTCACCGCGAATGACAGGGAAGGACAAGGCAGAGCAGCAGGCCAAGACAGCTTTAGTCGCAGAAGTTCAAGCAGATCTAGATTCATAGTAAATGGAAGTTCTACACTAGTTACAAATTTAAAAACGTACCTGCATGGACTACACGGTTTATTTACGAGTGCCACTTGTCTCATTGTTTTCCATCAGATGTCTGCTGGATTGTGGTAGTGTGTTCTACCGTATCGGTGCGGGTTTTGTATATTGTGCGTCGACAGAGTGACAGGTGGTGATTTTACTGGCAATTAAAAAAAAAAAACAAAAAAAAA A SEQ IDNO:216 Late LPZ-172GGTGCGATCCTAGTACAGGCGTTTGGAACAGAGTGGAGAATATGTGGAGTATTGGGGGATGCCCCCGGTCGTGTGTTGCTGCGTTTGGGAATTTGTATTTCTTCCATAGGCAACAAGTGATGTGTTATAATAGTAAAGAGAATGTTTGGGAAGTGGTGGCATCTCTTCCTGGAGACATGAATATTGTTACTTTGCGCAACAGTGTGGTGTGACAAGATATTTGTGAGCGGTTGTGCTTGCAGTGGCGGCGATCAGGTGTGTTACATGCTGGACAAATCTTGGGCGTGGGCTCCTATTGAGAGGTCACATGAGTTTGAGGGTTTTGCTCAGTCTGCAATAACTGTAGAGATATGAGCAAATTCTGTTGGGTTCACTTAATTTTGGGATTATTATAGTGCAGAGGGGAGCCGGGAAGTTTCAGTGTACAGTGATGGGCACCACATGTTGCCAGCATTGGGGGTGCCCTGTGAATATGATTTCTATAAGTCCGGATTTTAAATATCTAGGCCATCTATCTCATCCAGCCTCTGATTGTGTCTGTACTAAATATATCCTGTATATTCGTGATCCCTGGTTTTGAAGTGAGCAAGTTTTAGTGGAAGAGGATTTTTATTAAATATATATAAAGTTTCTGTATTCAGGGTTTTGGCAAAAAAAAAAAAAA SEQ ID NO:217 MiddleLPZ-173 GGTGCAATCCGCCATAAGAGAGGCATACAGGAAAAAGAAGTACCTGCCTCTTGATTTGCGTCCCAAGAAGACTCGTGCTATCAGGTGACGCCTTACCAAGCATCAGGCATCTTGAAGACTGAGAGACAGAAAAAGAAAGAGATGTATTTCCAATGAGAAAGTATGCAGTCAAGGTGTAAAGCCATAGGATTGAGCTTTCATGCAATTTTTTTGTTACTTGCGGGATGATATTGCCTATTATATTTCCGTCCACGTTTTTGGCAAATTCCGATTTGCATCAGAATTCAAGTTATGATAGGTGTTCTTCGCTTTGAGCAGTTGATATTGTTTATCTTTATTTCTCTTGAATTGCGAACATATTCTAATGCAATGAGTGGATTATTATATTGTGGCAAAAAAAAAAAAAAAAAAAAAAAA SEQ ID NO:218 Middle LPZ-174GCGGACGCCTCAGGATAGCGTTAGGGTTGCCTTAGGATAGCGTTAGCTCTGCCTTCTAAGGTTGCCGTCTTATCCTCCAGCGTCTAGGGCTTCCACTCCTAGGATTTCTCTTCCACTAAAACCCAAGACAAGTGGAGAGAAATCAAGATAGAAGTGTGTGTGAAATGACTCTTAAGTCATCTCCTTTTAGACTAAAACATTGAGCACATGTGGGGTTTATTTGGTTGCTGGCCGTCGTT SEQ ID NO:219 Middle LPZ-175GGTGCGATCCTGAAACAACATATTCCCGATGGCTCTTCCGAAGGAACCATTGCTCTACTGTGTGGCCCTCCCCCCATGATCCAAGATGCCTGCCTACCTAACCTGGCCAAAATGAATTATGACATTCAGAATTCGTGTTTTCAGTTCTAATTACACCCTTCTGGTTAATCAAATTGGGACATCCCCTCCCACATCCTGTTATTAATTAAGCCATAGTCTAGTGTATAAAATCTGTTGATGTGTACAGCATCAAGTTAATTTCCTCCTTTTCTGTCAAAAAAAAAAAAAAATAAAAAAAAAAAA SEQ ID NO:220 Late LPZ-177GGTGCGATCCGATCCTAAGCGGGTGCATATATATAATGACAAGCTGTAGTAACTAACTCTTGTCATGAGGCCATTGCTAACATAGCCTGTCCAATGCACATAGCAGTCAAAAAAAGCAAATAGCCGCCATGTTCCCATACACGAAGTAAGTACCCTCCCTATTGAGTCACCTTACCCGCCGAGAGAGATCCCAATTCCATGTATTCGGTTAAGTAAGCCCTGCCAGCTATGTCCCACCCATGAAAGAAAGTACTGATCCGAGTGGATCGCACC SEQ ID NO:221 LateLPZ-179 GGTGCGATCCAAACTGTGGTATCGGTGGAGAGATTAAGCAATTTATTGGAGTAGCAAGTACGCTGAATTAAGGGGGTCCATATTCAAGCAAAGGTTCCTTTGGATGACTATGTGTTCTGGAAGTGTTTATGGATCAATCATCTCATAAATTTTGGTAATATATAACAGAAGATTATGGCATCCAGTTAGGATGGTAGTTTCATTGAGGTATAGTAAAAACTACACTAAGTCTTGTGTTGCCACCCACTTTTGAGAGAGGTCAGGAGGTCTCTTTGTGAATCATTGATAACTTTATGAGTGGTACCTAATGAAATATTTGCATCTTGAATATATACTCAATTGATCTTACTTGTGGATCGCACC SEQ ID NO:222 Late LPZ-181CAATCTGTCTGCAATTGATATTATTGCATCCAGTAAACCAGATACACATTCACCACAACATTAGAGACTCTAGAAGTTCCTTTGGCGACAGGCAAAACTCATGATTACAGATAATTGGAGTTTCCTCTAACCAGAGTCAAACGATCTAAAGGGATTTGTCTAGTCCTCCATTCCCTCATTCAATGAGGCGATGGCTTATGCCGTGACAACAGTTTCTATAGTTGCATCCGCTCCTGTTGATCCCACAACATTTTTGGTGTTCTCTGCATCTTCTTCCTCCCATATCTCTGGCAGGGCTTGTCTAATGTTGTGAATACTTGCAAGGGCAAAATCTGCTCCCTCTGTTCGGATCGCACC SEQ ID NO:223 Late LPZ-182GGTGCGATCCTCTCAGTTACGAGCTCAATTTCGACCAGGGGTCTCGGCAAATTGAGGATCATGAGAAGCAGGGTATGCCCTTGAATGCCCTGAAGCCAGGGGAGTCTCAGGGCAATCACGAATGAAACCTGACAAACCCTAAGAAAACCCCTAGAGCGTGCCCTGCAGAAAGGGAATTCTTTTTGAGGCCGGCGGTCTTTCTGTCGTCTTCTCGCAGCCGTA SEQ ID NO:224Late LPZ-186 GGTGCGATCCAGCAAGAGAACGAAAAAGGTATGAGAATCTATGAAATATTTGTACATCACTGTATTCATATGAGGGCCTTTTTTTACAATGCGGTAGGGTTGTTTGGAGAATAGAACCTGATTAAAATGTAGATGGATTCAAGCTTTTAGTGAAATGAGGCTCGGAACGCAAGTATGCTGTCCACTTTGAGACTCATTCTTCTATAGTATCTGAAGCCAAAGCC SEQ ID NO:225Middle LPZ-189 GGTGCGATCCCATGGGATAGTTGCAAAACACACAAATTTGTTGTGAAAGAAGAGAGACACGCACAGACAACCATATGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCACAACTCTGCTGCACCATATATTTTCATATAGCTGAAAAACTTAGCCATCCTTATATTGCAGGATTTCCGCATTCTAAAACAGGAAAACTTTCCTATTCACAAAAGTTATATTCACAACTCTGCAACTGATATGAGTGCCACTGCACCATATATTTTCATAGCTGAAAAGCTTAGCCAGCCTTATATGCAGATTTTCTGTTTTCTGAAACAGGAATTACAGGATCCATCACTGTACTCCTTTGCCTTCCTTGCCCGTTCATCATCCAAACTACTAT ACGGATCGCACCASEQ ID NO:226 Late LPZ-194GGTGCGATCCTGCGAGAGCCGAGGGTTCATTTTCCTTTCGACAACGACGTTCAGTGGCGACCAGAGTTTCCCAATCACTTCAGCGATTCTATTCCTTCGTTGTAATAAAGCTTAAGGAATCCATGCTTTATTCCTTGGAAGGTTTGAATATTTATATTTGTTGGCATTAATGCTATATACATCTATACTAATTTTGGGTTGTTCTAAACTTGTTTTGAATAACTTAAA SEQ ID NO:227All LPZ-195 GGTGCGATCCATGGCAAAGAGCTCGTTCAAGCACGATCATCCTCCAGAGAGAAGACAAGCTGAAGCTTCTCGGATTCGAGAAAAGTATCCGGACAGGATTCCGGTTATTGTGGAGAAGGCTGAGAGAAGTGAGATACCTGATATTGATAAAAAGAAATATTTAGTCCCAGCAGATTTGACTGTTGGGCAATTTGTTTATGTTGTCCGAAAAAAAAAAAA SEQ ID NO:228Middle LPZ-196 GGTGCGATCCCCTGTATTCTTGAAAGGGTTATAACGGAAGATAGCATTTTGCTCAGATTGTAGACAGTCTGCATGATTTGTCAATACTACTATTTCGCATTATTTGTTAATACTACTAATCCTTGTACTCATCTAGACTATTTAATTATTAAATTCTACAGTTTCTTTCTCCTAGATGGCAAACAATATGAATAAAATGCCAATAGTTTTGGAACTACTCCATTAAGAGCTTTAGATGATTATCATTCATCATTTGCCTGTTTTGAATCGTAAATGAATGTGTCACGGTCTTCTTTTCTGTTAGTCTCTATGCTTTCATCAGAAGAGTCTAAGCCAGTTACTGGAAGCTATTTGTCATCTCTTTAAACATTGTTTCCGTGCCAAAAAAAAAAAAAAAAAAAAA AA SEQ IDNO:229 Late LPZ-197GGCAGAACTTCCAAAGTCTAGTATTTGATTAACTAATATGATGAAGACACTCAGTCTATAACATGACGCCAGAAATCAGACCATATGCATGATAACTAGCACGATTAAAATACAATTCGCAACCTTTAATACACTAAAAACGTTTACTGTATAGTCCACTCAGAACATTTCGATAGTATTGTCAGATCGACTTATTTAGCTCATATTCAGCAATCTGAACTGTACGATGCGGCTCATTCAAGGGCATTTGGGTTTGCCCTTGGCATTCTTCATATCCCGATAGCAAGGACACGCGTTCTTGTTGCCATATGTCCCTGGGGGATCGCACC SEQ ID NO:230 EarlyLPZ-198 GGTGCGATCCACATTGGCCAGGCCGGTATTCAGGTCGGCAATGCCTGTTGGGAGCTTTACTGTCTCGAGCACGACATTCAGCCTGATGGACAAATGCCAAGTGACAAGACCGTTGGCGGTGGAGATGATGCATTCAACACATTTTTCAGTGAGACAGGTGCCGGTAAGCATGTTCCTCGT181GCCGTGTTTCTGGATCTGGAGCCAACTGTCATTGATGAAGTTCGAACCGGCACATATCGGCAGCTTTTTCACCCAGAGCAGCTGATCAGTGGCAAAGAAGATGCCGCGAACAACTTTGCTCGTGGCCATTATACCATTGGTAAGGAAATTGTGGATCTGTGCTTGGATCGCAGC SEQ ID NO:231 Late LPZ-199GGTGCGATCCCAGCATTGGATGCATTTCTAGCACAAAGCCATCTTGACTAAAATAGCACTGCGGGCAACTGCAGTCCATAACTTTCAGAGCATTGTTGCTGCCTCAATTGTATACCAATCCATATTCTAAAAATTAGACCTGGAAACCAGTCAGAAATTTAATGTTTTCTTGCAGAAAATGCCCTTTTAGAAAAAGGAGAGAATAACTGCATTCAAGTTCTAACTCCCAGACATAGCCTGGCAACGTCATTCATCAGTTCGGATCGCACC SEQ ID NO:232 E,L LPZ-201GGTGCGATCCAGAAAACAGCACAAGCAATCTGTAAGACCAATATTATTATCATCTCTCACTGCTCGTGAACAAAATGCTGGTTCATAGCCATCACGAAGGCTAAGGCTACTATCCAGCCAAACTGATCTOCAACAATAATTTCATAAGCTTAAATAAATAGTCCATCCAGTGGATGGAGCCAGAAAGCCATAGAAACTTCAAATACTTGTGGTATCAATCTCTCCTCTGTTAAGGGAGGTATCAGATCAGAAGCACTAATCAAATGCATACATAAATGCAGTAGACTGCAATAAAACAAAATCTGCAGATAGCAACTGAGCGCTTAACGAACGGAAAAGAGTTTAACTTGATCTATCACAGGATCGCACC SEQ ID NO:233 Late LPZ-202GAAAATGGGAGCCTCAAATATTCAAAGCCTCATCTCAAGAGTCTCAGATTCGGATTCATTTCATTTGGTTCGTAATAAAATAATGCATCAAATAGTTATTATCCACAAAAATGGGAGAATTATTACAATCTGTCTTCTCAACATAAAGTCATAGCATAGCATAGAACCACACCACAGTCGTCATCATTTGTTTTGTTCACCACCGAAGGGGCTCTTTACAGCGTCCATGAAGCCCTGTGTAGCACCCTTCGCCTTGTCCCCCGCCTGTTGGAAGAAAGAGCCAGTTTGTTCTTTCCCCTCTTGGGCTTTTCCCGTGATGGATCGCACC SEQ ID NO:234 LateLPZ-203 GGTGCGATCCTATTATAGAACCATGACTCTTGTCGATGGGGCATAAACTTCTCATTCTTAGGCGTGCCTACTGTGACTCTTGCCGATGTGGCATAAACTGCTTATTCTTAGTTGTGCCTTCTGTGCAGAACTTGTTGAGTCGGTGGATTACACTGAC SEQ ID NO:235 Late LPZ-204GGTGCGATCCATTAACTAGATTAACGATAACATTCCTCTGCATCCAATCCAATGCTCATCTAAATCTACTTCTACTTAGATCTCTGCCTCATCTTTCTCCACCTCCTCATCCATTCTGAAATATTAATTTCTGCATAGATTTTGTTAGGGTCTAGTAATCATTTTCATGAATTTAAATCTGTTCTAGTCTCTTATTATTATGCTGCTTATGCTAGCATCAGAACCTGTGTATAATTCATTCATGTATATATTGGATTACACAAATTATACGGATGCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA SEQ ID NO:236 Late LPZ-205CTTGAAGCTGATATGTTTGAACCCGAAATTTTGTTACCCAACTCCAGTGTACATTGTGTCACTGTCAAAGAGAACATGAGAGCTGCATGCAAGCTTTTGCATGATAGATAGATTACTGATCACCGAACATTTCTTACTCTACTTTCCTCTCCTATCCCCAGTGATTTTTGGGCATTTTCTATACCCTTCGGATCGCACC SEQ ID NO:237 Late LPZ-206CTCATGAACAGCAATATGATGCATTCCTCTTATACACATTTCATATATGTCACCCTTGCCGTCATGGCTACTCTAAGAAGAGCAAAACAGACCCATTGAATCTTTACACGCGCTTGTTTATATGAATACAAATAATTTAGGCGTTTCTTTACACGCCCTTGTTTACATTAATACAAGTGATTTAGGCGTTGTTACCAGAATAGTGCCACGGATCGCACC SEQ ID NO:238 AllLPZ-207 GGTGCGATCCCAAGATAGAAAAGGGAACTATGGTCTCGAGGAGTGTCAGGTGCTACAGATCACAATATACATAAGGGTCTGATAGTAGTACTCGGCCCAATGTTTGAGGGCTCTAACTAAGGAGGATCAACCGTACCCTTAGCCGTAAAACCCGACTACCCTATCGTACGGGCGAGTAATCTCTCTGAGTGTTGTTCTCGGTGTATCGTAGCAGCAACACGGCTGACGGTTTATCTATGGTGAGGTTTCAAAGGAGCTAGGGGGCTTCCAATATACCCAGAGGGTACTTGGAAGACAGTTTATACGCGGTTCTGTCTAATGCGCTACTACTCGAAGGGGTACCCACAGGGGTACAAGAGAGTGCAACAAGCATGACCACCCCTTGTATTTCTTGCATGTATGCCTCCCCAAATCCGCAGGTTTATGCGCTCATTGACAGATTCCGTGGTTTAAAGATGCCGGAACATGTCTCTAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA SEQ ID NO:239 E,LLPZ-208 GGTGCGATCCTCCTAACCTGCAATGTCCTTCCTGCAATTATCAACAGAAATTAGGTTTATTTTTCTTTTTGTCTTTTCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAGTAAACGACCATTTCAAACGCCATTCAAATGCTATGAATTAATGTTGAATTAATGTTAGCATTAAGTCTTTTAACATTTTATGTAAGGCATATATATCGTTCCAACTACTCTTACAATACACCTGCGGTGTACTCCTGCCACCGCATGTACCACCGTTACATGTACGCCTGCCAGCACATCTAACAGGTGCCAACTCCTTTGAACTCATCGTCGCCATTTTTGTATGCATATTTGAACTCATCGTCGCCATTTTGGTATCTTCACATATGGCCAGTCCAGGATCGCACC SEQ ID NO:240 Late LPZ-210GGTGCGATCCAAGGAGTGGGCGTGCAATGCGTCGAAGATAGCCACCACTGCAGGGGCGTGGCATGCTGCCGTGCTTCCCACAGGGAGATCAACACCTGCACCTCCGCCTCCTTCCGCGGTTACCACGAG SEQ ID NO:241 Middle LPZ-211GGTGCGATCCAGCCACAGAAAGATTGGTTTACTCGATAATTGAACGGTAGACTTTGTGCAGGTTTAGATTGTGTACATGCTGATCAGTATTGTCTACACCATTTTCAATCTTGTTTAGTTCTATGGTAATTTATGTAACAAATTCAGCGATGTTGGGGAAATTGGTCACATCAGCTTTGTGCCTATATATTCAAGTAAATCAGGGGATCCATTAATACTGCTTTTAATAATTGGGGCAAAGTTGTGGGATGACTGCTTCAGCGGAATACGTGCTTTTCATAGTGCTGTATGACATTTTGTTGAATATGAATTTCTTTGTGATACAGTTGCGCGAAAA AAAAAAAA SEQ IDNO:242 Middle LPZ-212GGTGCGATCCATGCCAAGAGGGTGACCATCATGCCCAAGGACATCAGCTCGCTCGCCGCATCCGTGGAGAGAGGGCATAAACAGTCAGTCAGATCCAATGGTGTGTTTTCACACCACCATATGTTTCTTTTACTAAATTTGTTAGGTCCCTTCGGTGGGTCTTTTCTTTCCCCCGATTTTAGTATTTTGTTGTTCTTCTGAGTTTCATCATTGCAAGTACAAGATGCAGAATTGATGGTTATTGGGACTTGGAGACTGGTTATTGCTATGTAGAGTATTTATATTAGACAGGTTTCACTTGAAGATATAAAATTG SEQ ID NO:243 Late LPZ-213GGTGCGATCCTCATGTGTATAACCGAAGTTTGCGGGATTCAGATGGTCAGTATCTTAAATGTCCAACTTTCGGTACGAATGGGGTGCGTTCTGAAACGTGCCACGAAAGAGGTGTTCAGGATCTGTCTGAGGCATCTTTCCGGTATTTTCCACTTCCATGGTATGAGAAACTTTCGTCTTGTTGCAG SEQ ID NO:244 Late LPZ-214AGGAGACACAACTTTACGAAAAAGTTCAATCTGGAGTCTTCTAAGTTTTTCAGACTCTCTAAATATGAAAAGCGCCGAGTTTCTCCTATACTGGACTCGTTAAAATTTTACAGTAAAGGACCTGTTCTATTACAAACAGGAACGGACCGCTCCTCCTTAGGGATCGCACC SEQ ID NO:245Late LPZ-215 GGTGCGATCCAGCAAGAGAACGAAAAAGATATGAAGAATCTATGAAATATTTGTACATCACTGTATTCATATGAGGGCCTTTTTTTACAATGCGGTAGGGTTGTTTGGAGAATTAGAACCTGATTAAAATGTAGATGGATTCAAGCTTTTAGTGAAATGAGGCT SEQ ID NO:246 LateLPZ-216 CTCAACATAAAGTCATAGCATAGCACCACACCACAGTCGTCATCATTTGTTTTGTTCACCACCGAAGGGGCTCTTTACAGCGTCCTTGAAGCCCTGTATAGCACCCTTCGCCTTGTCCCCCGCCTGTTGGAAGAAAGAGCCAGTTTGTTCTTTCCCCTCTTGGGCTTTTCCCGTGATGGATCGCACC SEQ ID NO:247 Middle LPZ-217GGTGCGATCCCATGGGATAGTTGCAAAACACACAAATTTGTTGTGAAAGAAGAGAGACACGCACAGACAACCATATGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGGGACCAAATATTTTTCAATACAACGCCATGTGACATTTTTGTGCTTCTTGTTTTTGATACATACATTCCAAAAACTGAACACTCGATGGATACGGTGATGATGCAGCTACAGCCATTGCATTACAGATGTTATTAAATTAAATCAATTTATTATGTCATCACACCAACCCAAACAATAGCGCTATTATGTCATAGAATGGTTGCAGTTACAAGATCTGCAAACAGATCAATGAATCATCATGCCCCTCTATATCTCTTGTCAAACATCAAGATAAACCTAATTTTAGGACTGGACTTCCTCAATCATATCACAATGGCAAACTCAGCCTCATGTCC SEQ ID NO:248Late LPZ-219 GGTGCGATCCTGGACTGGCCATATGTGAAGATAACAAAAATGGCGACGATGAGTTCAAATATGCATAGAATAAGCGTTCTGTAATTGGAACGGCCATAGGAGTTGGCACCTGTTAGATGTGCTGGCAGGCGTACATGTAAACGGTGGTACATGCGGTGGCAGGAGTACACCGCAGGTGTATTGTAAGAGTAGTTGGAACGATATATATGCCTTAACATAAAATGTTTAAGACTTAATGCTAACATTAATCAACATTAATTCATAG SEQ ID NO:249 E,L LPZ-220GGTGCGATCCCATGGGATAGTTGCAAAACACACAAATTTGTTGTGAAAGAAGAGAGACACGCACAGACAACCATATGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTTTTTTTTTGTGAAGTGACAAAATCTAAACCAAAGATTAAAAGGCTTTGGCTTCAGATACTATAGAAGAATGAGTCTCAAAGTGGACAGCATACTTGCGTTCCGAGCCTCATTTCACTAAAAGCTTGAATCCATCTACATTTTAATCAGGTTCTAATTCTCCAAACAACCCTACCGCATTGTAAAAAAAGGCCCTCATATGAATACAGTGATGTACAAATATTTCATAGATTCTCATATCTTTTTCGTTCTCTTGC TGGATCGCACCSEQ ID NO:250 Late LPZ-221GGTGCGATCCCAACCAGGTGTCCATGCAATATATGGTGAGCATCAAGTTTGAGGTGGTTGATTGAAAGTTACAAATTGGTGACATCTGAAGTCTCATTCAGTTATGTTTTTGTTATAAAAACCATAACCAATTTTGTATATAAGATCCATAATCAATTTTGGCCAA SEQ ID NO:251 LateLPZ-222 GTTTTCAAGAAGAGCCTGACGGTTTCCTCGGCGGGATGACGGAAACAGGAAGCGGCCGGCCGGTTCCGGACCCTCCGCAGGCGGAGCATAGCATTTTGCCGGAACCACCGCATGTCGTGCACCCAACATCCGCGTCTGACCAGCGGAGGCACATGCACCCAACCCTCCCGGTTCCATGCACCTCGGGCAGCGCGGCCACCCGCCGGCCATCGGCTTAT CCATCATGGATCGCACCSEQ ID NO:252 Late LPZ-223TGGGCGAATCATATGGCTTGCATTTTCATTGTAACATGTATACGTTAAGGATTATCATAATGCCTCCAAAACCTTGTATCTTCGTCCTTGCCACAATACATCCAGGATAACTAATGGAAGCTTGACATGTCTTCACCAGTAATAATATATCAACTATAATACATGCCATTCTTTATCAGTTTTGAACAAAATAATCGATTTGCATTCTTGACAAAGAACCTCGCGCATAAAAACAAATAAATTCTCATAATGCCTCCCAAACCTTGTAGTCTGGGCCCTCAGTCGCCACAATCCATTTAAGAGGAATTTGGGGGTTGATAGTGCCCAGGTCCAATCTTCATGAAAATTCGTTCATCAATCTTTGCTGCATACACATCTCTCTCTGCTTTCACTATCTG GGATCGCACC SEQID NO:253 Late LPZ-224CCACTATAATGAACATTGATATTACAAATATAATATACATAATATTACAATTCAAATCATTGACAATGAGCAGGCACTACTTGCAGTGCTTTGGAATTCAGACTTCTGATTTGCATTAATTCTTGTAGACGCTTTTCTGGGAGGGCAGGTTTTCCGCTTCAGAGAAAACCACGTACAAAACGATATTAAATAAAAATAGACACATACAAAAAATACTTCATTTTTTGCTCTTTCCATTTGGTTTCTTCCTCTATCTCCATTTTGGAGGGCTTAAATGACTCAAATTTAAAAGTCAACAACAGAGTGCAGCACATTCTATTAGCTTTGCTGTAAATATCTGAT TGGATCGCACCSEQ ID NO:254 Middle LPZ-225GGTGCGATCCGCATTAAGAGAAGCATACAAGAAAAAGAAGTACCTGCCTCTTGATTTGCGTCCCAAGAAGACTCGTGCTATCAGGCGACGCCTTACCAAGCATCAGGCATCATTGAAGACTGAGAGACAGAAAAAGAAAGAGATGTATTTTCCAATGAGAAAGTATGCAGCCAAGGTGTAAAGCACAGGATTTGAGCTTTCATGCAATTTTTTTGTTACTCGCGGGATGATATTGCCTATTATATTTCCGTCCAAGTTTTTGGCTAAATTCCTATTTGCATCAGAATTCAAGTTATGATAGGTGTTCTTTCGTTTTTGAGCAGTTGATATTGTTTATCTTTTATTTCTATTATTAATCTTCTAAGTTGGATCGCAC SEQ ID NO:255 Late LPZ-226AAACAGACAAATATAGAAATATGCATACATAAGTCCCTGCAGAATTGTTTTCCGCAATGAATTCTGGTTTATGGCAACATTACCTACTTAGTACTAACCCTAAGATTATTTTCAGCTCTGATAAGTGGCATACGTGTATCAATCTTGCATGAGTCTATCCCTGTTTTAATCTTTTGTTGGGATCGCACC SEQ ID NO:256 Late LPZ-227GTGGAAGCTTCATTGTAAAACACTACTGGTTTTGAGAGAACAAAATATATACGCTAGCCGAGTGGATTATAACAAAATATAGGCTTTATTTCTATTGGATCGCACC SEQ ID NO:257 LateLPZ-228 GGTGCGATCCCATACATTAACATAGCCATCACAGCCCCCAGTGGCAAAAGTACCATAGCTGCAAAACATTATAAAACTAACATTCCTACAAGGAAATAAAATACAACTAAAAAAGCAAGCAATAGGCATTAGGGGAGGGAGAAGCTAAAACTATTAAGCAACTTACATGGGATGAAAGGCAATTGCGTTTACTGGATAAACAGTATCTCTGCCAGCCTCTGACTTGCGATGACATTTAAAGGCATATTTTTTAAGCTTGACCAGCTTCAGATACATCATAATACTCCATAGCCATGCGAGCTTCCACAGAACTAAGGGGCAAAACCTGTCCATTGG ATCGCATCA SEQ IDNO:258 Late LPZ-231GGTGCGATCCAACTGAGAAGGGTGTTGGTGGAAAGATGACACCAAGTGGGTTCTCTATTCTCCAGAGGATGCAAGAAAAATTCTGAGAGCAAAGAAGAATGGGGACTCAAATATTACGTTGGGTTCTGTTAAATCTGCCAAGTACCCTTCAGGAAAGCTTTATGCCATAGACCTGGTGGCCATGAAGCAAACCAATGTAAACACTGGCTTCTCCAGAGATATCAAAATCATCAATTCTTGCCCTACTGATGATCAGGAAGATGTAGAGTCTGATGAAGAAGATGAATTATTCACATTCTCTCGTCCTGTCAAAGTTGAAGTGATTAACCAGAGCAGGAAACCTGATAAGATTGTCAAGATGGTTCCTTCTGTCACTGTAGACCTTGAGAAATTGACTTCTCAATACCTCCTGGAGGATGAGTGCAATTTGGTTCTAAAGCTTCCCAGGGCTGCAGCTGCCCAATCGGATCGCACC SEQ ID NO:259 Middle LPZ-233GGTGCGATCCAGCTAATCAAACTTAATGGAGAGCCCTCCCAGGAAGAGTAAATGGTAGTCACTTGAAGCCCTACACGGGTGGGCTGGCGGTCTGACTAACTGACCAAAACATAGTCTTCGCGACCCAACAAGCCAGACAGAGGTGTGGGACTATAAGCACAAGTACTAGAAGCTAGCATCAAAGTAGAGAATTAAGTTAGATACAGATGATTCAGAAGCAATGGAGCAGATCCAGACCACGGTAGCATGGTGAGTTACGAACCTTCACGCCACACCAACGCAATTGGTTAAGACTTCGCACTAGGATCGCACC SEQ ID NO:260 Late LPZ-234GGTGCATCCATAGTTCCTTTGCTAAGCGACTACTCTATCTCTTTTGACATTCTCCAAATATTGGGTCTTTCAGTTCCTTCAAATGCTAGAATCATATCAACATGGGATTTAGTGAGGCCGCAATACTAACCAGGGCATTAAAATAATACATTTCATTGATCCTATTCCCAAAACATTTCCCGCTATCGTACGTTGACTCAGCATATTTAGAGCAATTCTCTTACAAACCTTAAGAAGGTTGTTCATGATAGTCTTTCCGTCTGCAATATTGGATCGCACC SEQ ID NO:261Late LPZ-235 GGTGCGATCCCACCCAAGAGTTAAATTCACTTCTCCGCCTTTCTGAGGAAGAGCACTCTTTGGATGATATGAAAAGTGGTCCACTCTTAACCGTATTCGGAACCCTGTTCCGCGGACGGTCGTATGGCGTAACCGGCGCAGACATTTTATCTCCTCACACAATATCAACATTCAAGTCCCCGCTGTTCCCCGTTGCCTTTCTCTGCTCCCGACCGTTAAACAAGAACGACCACAAGAATGAACAACACCGCAACCGAAACCTGACCCTCCACGTTGTCTTCGGTTCGGATCGCACC SEQ ID NO:262 Late LPZ-237GCGGACGCCTGGCAAAAACAGAGGGTATGCTCAAGCCTTACAGAAATTGAAAAATAAGAGAACGTATGACCATCAATCTCAATCTCAAGAAAAGAAGTTGCAATACGACTCCAACACTTTTGAAAGTTGGAGGTTTGCTCTTTCTAGCGTTGCAGACATGGTTGGTTTTGAGCTGGAAGCGTGTAACGGGCACTTTACAGTTGCGGGAATTGGAGATTGAGGACCCCCTCTCAAACGTCGATAGGGAGGCTAAGCATCTATAGAGGATTGTGATTGGTCCTTTTCCGCTACATGGAAAGTTTGTCAAACTCAGAAAATTACCAGAAGAATTCTGTCGT CTTCTCGCAGCCGTSEQ ID NO:263 Late LPZ-239GACGTTGTAAAACGACGGCCAGTGTAAAGAGCAGCCCCGATGCGCCGAAGCTCGCGAGGGAAAAGCTGCAGAAGATGGGACCGATGACCAAGAATGAGATCATCATGAGCGGCACGCTACTGGTCACGGTGGGTCTTTGGATATTTGGGGGTAATGCTGAACGTGGATGCTGTTACTGCAGCGATCCTTGGTTTGTCTGTCCTACTCTGCACAGGCGTCCGC SEQ ID NO:264Late LPZ-240 TACGGCTGCGAGAAGACGACAGAAGCAGAACCTGCCAATATAGGATCAATTGAATGTTGTGGGATTGCTGCATGCCCACCTTTCCCAGTTATTACTGCCTTGAAGAACCCACAGCCAGCGAGTAAGGGCCCGGGTTTCGAACCAATCACAGATGTAGGATAATCGCTTGAAACATGCATAGCGAATATGCCTTCCACATTTTCCAGTGCTCCCTCCTCTATCATTCTTTTTGATCCTGCACCTGATTCCTCTGCAGGCTGGAAGAGTAATATGACAGTTCCCTGTAACAAATGCTGACGTTGTTGCAAAATCTTTGCACCACCAAGAAGCATGGTAACATGTGCATCATGTCCACAGGCGTCCGC SEQ ID NO:265 Middle LPZ-241TACGGCTGCGAGAAGACGACAGAAAAGAGGCAAACCGAGCTCGACACCTCCACTCAGAGCATTTGCAAAAATCCACAACAAATCTGGAGCCAAGGTCTTTCCCTCATTGAAAACATTTATCGGACACATCAATGTCTGTAGTCTTTCCCATGGTCCATCCAGAGTAATCACGGGAAGAACAATGCACTTCAGTCAGAATTTTTGATGACAGCTATCAGCTCCTGATCCTTTGAACCAGGTATATAATAATCTTGACCTGACTCCTGTTTCAACAGTGTAGAGGTTCTGTCAACCTCAAGCAATGAATCGGCAGAACTTCCATTTGCTGTTTTGTCAATACAGGCATTGTTTTTACCAAGACTGTGACGCATCTTCTGTCCTTGTCTATACAGTGCAGTTTGTTCAAGCATAGACTTATGTGCTAGAACATGTCTTCCTTTTAAATTGTAAGAGAAATGTAGGGGTTGACTGCTTTTACTGAGGCGTCCGC SEQ ID NO:266 Middle LPZ-242ACGGCTGCAGAAGACGACAGAACCCTGGCTGACTACAACATTCAAAAGGAGTCTACCCTGCATCTGGTGCTCCGTCTAAGAGGAGGCATGCAGATTTTTGTTAAAACCCTTACAGGCAAAACAATTACTCTGGAAGTGGAAAGCTCGGACACTATTGACAATGTAAAAGCTAAGATCCAGGACAAGGAGGGAATCCCACCTGACCAGCAGAGGTTGATCTTTGCCGGAAAGCAGCTAGAAGATGGTCGTACTCTGGCCGATTACAACATTCAGAAGGAGTCGACCCTTCACCTGGTGCTCCGTCTCCGTGGTGGCTTTTAGGTTGGCTGTTGTGTGTCAATGTAGTCTGGTGATGTTCAGTGGTTTTCCTGCTTAATCCTTTTTATGTATGCATGTGTTTGTTGTGTTTGTGTTTTGTCTCTATGTTTTTTCTACTTGGTTTGTCGGTCGGTTGAAGCCCGGCTGGTGTCCTGGTAGGCGTCCGC SEQ ID NO:267 Middle LPZ-243GCGGAGGCCTGGACAAACACAGAAGGCGAAGTAAAAGCCAGTCTTACTTTTCATGTAAATACTATCAAACTGCATGGCCGTTCCGCTGGTTGGCAATACCACACCTGCGCCGGTAGTGCCAATGAACACTGCACCGGCAGCTCTTTCAGAAGTTGCAGAGGACTTACCATTTTAATTTTCACGGCATCCCGTCAAACGGCGGGATGCTTTTAATTTTTTAATCAAAAAAAATATTAATTATGGCACACAATATTGTTTTCAACGAACAGACAGGCAAACACAGTTTCTTTAGTGTAAAAGAAAAAGCATGGCATGGTTTGGGGCAAATTGTACAGGACTATCCCAACAGTAAAGAAGCATTGCAATTTGCAGGGCTTGATTTTGAAGTTTGCAAAAGGCCCAATATTCACAGGCTTGATAATGGTAATGAGATTATTTCTACCAGTTCATTCTATACTTACCGTCCTGATACCAACGCCATATTAGGCGTCCGC SEQ ID NO:268 Late LPZ-244GCGGACGCCTGAACATAGGAGCATTCTTAAGCATATCAGGTATAACCATAAACCTGACTTTGCTGCCCCGAATAAAGACATGCTCCAATTGGGATACTTTTCCATCCTTGGCGTGTNTGTGATGCCCTCGAGCTGGCAATTCCAGTTATCTTCGCATTCGATCATGCTACCCCTGTACAGCTCGCCACTTTTGAGTTCAACTGTCACAACATGCCCGGCTGCTTCATGGAGCAACTTCACAGGAATCCCCAAACTTCTGCTCATTTTTTTGTCACTGCTCAAAAACCCTAAACCCCAGATAAAACCCTCGGTTCTGTGCCTTTTATCCCCGGGTGGCTTATTGTTGCAGTAGTTGGCAACGGCTAGACTTACTCACATTTTGATTTCAATCTTTCTAAGTTTGCCCTTTTGGGTTTTCCTCACAGTAGATCCTATTTTATGTATTTTCTCGTCTTCTCGGCAGCCGTA SEQ ID NO:269 Late LPZ-246GCGGACGCCTGCAGGAATCGGCCGATTTGCAGTTCGAGGCATAAGCGCATCGAGGTCGCGTTCGATGTAGCAATTAAGCGCGCATGAACCGCCGCTAAGCAAGCCAGTCCCAATCAAAGCACATGCAAAGCGGATGCAATCAAATCTTCCGTTGTAAGCAAGCACAAATCCAACTGCACATGAGATCACCACCATGAATGCAATTCGAGTGCGAGCTAAATCCCAAAACGCTGCGAGTGTCCCCTGAAGGCGATTCGTATGTAATATTTGACCGCTGCTCAACACAAGCAGTACTCCAAACACCAGTGCTTCCGCCGTCAATTCTGTCGTCTT CTCGCAGCCGTASEQ ID NO:270 Late LPZ-247CTGCGAGAAGACGACAGAACACAGACACAAAATTTGGAAACTACAGAAAAGACCATGTCATGAAATCTTCATAATTGGGCTTCAGATGCAGAGGGGGTCGGTTTTGGATTAAGCAATGGCTGAAGTGCTTTGACAACAATACTCATGTTAGGACGAAAATCTGCTTCATACTGCACACACAATGCCGCAACAGCAGCCATCTTTGCAACAGCCTTTGGAGGATATTCACTCTTCAACTTGGGATCAACACACTGCTTTACTTTGTCTTCACTCAATCTTGGAGTTGCCCAAGTAACAAGGCTTTGTTGTCCCCTAGGCATTGTATGGTCCACAGGCGT CCGC SEQ IDNO:271 Late LPZ-248TACGGCTGCGAGAAGACGACAGAAAGAGACAGGCTTGGACTTCGTGGCCTTCTTCCACCACGCATTATTTCTTTTCAGCAGCAATGTGATCGTTTCATGGTTTCTTTTAGATCCCTGGAGCATAACACTCGAGATGGTTCAGCTGACTTAACAGCTCTGGCAAAATGGCGTATTCTTAACAGATTGCATGACAGAAATGAAACACTATACTACAAGGTTCTTATAGATCACATTGAAGAGTTTGCTCCAATAATCTACACTCCAACTGTAGGATTGGTTTGTCAGAATTATGGTGGGCTGTCAGGCGTCCGC SEQ ID NO:272 Early LPZ-249GCGGACGCCTCAATAGTATGGAAGGGCAGCTGCACTACTCAGCATGAGTGGAGGCCTAAAAGTTTTGTTAATCTTTCTGGTGAGGTGGACACCAAAGCCCTTCACAACAGTGCAAAGGTGGGGCTATCTCTGGTTTTGAAGCCTTGAAGGATATGCACTATTTGGTACAGATTTAAGCGAAGGTCTGTGCCAAATTTTTATTGGAATTTTTGAGTTTTTCCTTTCAGAATAATTATTTCAATGCCTGTGTTTTCTGTCGTCTCTCGCAGCCGTA SEQ ID NO:273 LateLPZ-250 GCGGACGCCTTTTGCCCAATTAACATCCCTGCATCTGCGCATTAAAAATTGATTGCAGACCTGAGGTTTAAGTGGAAGCTTCTTCCACCATCTCTCCCCTGTTTAAGGAAGACCCGAAACCCTAGCCACTGTCTCCTCTGTGACTTAAAATTCCAGTTCACCAACCTTAACTCTGCGTCCGTTAAAATTCTGGGCAAACTGCACTGCCAATTGGTCATCATATCCTCTGAATTTGGCAAAGAAAACATAGGTCATTCTGTCGTCTTCTCGCAGCCGTA SEQ ID NO:274 NDLPZ-251 GCGGACGCCTCGTCAATCCATGGTTGTAAACATGCCTTCAAAACTGTTTCCTTATGTCGCACAATGTCTACATGTTCCTTGAGCGATTTTTCCTGCTGCATTGCGAGCCTCTGTGTAAGTCCCACTATCTGCGCTGTCCCTTTTACTTCATAATACTTCTGTCGTCTTCT CGCAGCCGTA SEQID NO:275 Late LPZ-255TACGGCTGCGAGAAGACGACAGAAAAAACTGTATACGAGTAGGCAGCGAGTCCTGGCAGTATGGGAGATTGAACTCCAATTACATTTAGTTACAAGTAGCATCAACAGTGACTGAGCCAAGAGCTCTACACAGAAAAATAAAATAAAAACTGTATATATTTACAGGAGAAACCCCTATGGCCTCAGGGCCTGAATAAATCAATCGCAGCGGTGGTCGATGTGGCCTTTTCAGGGCTGCAAATCTTGCAAGGGGAAGCCATCATCCTTGTTCCGTATCCTTTTTGAGGGATAGCGAGCCACGCAGCCAAGATTTGAAGCGATTGAATACTTTGGGGTGTCGAGAACGCACCAGAACAATGCCACTCGAGAAATACTACTGTGATTACTGTGACAAACAATTCCAGGATACTCCCTCCGCTAGAAAGCGACATCTACAAGGCGTCCGC SEQ ID NO:276Late LPZ-256 GCGGACGCCTGTACCGTATTGGAATTCTAAACCCTTCCTTGGTATAGGGTTTTCGCCACCCTTGCGTTCATTTGGTTTTGTATTACGTCCGATTCCTCCGTCTGCGAGCTCTCTGCAACTTGGCAATTTCATTGTGATTTTATCCTATGATGCTTCGTATTTGTTTGAAGCTCGTCCTCCTAGTTCTCTGTGATACCAGTTGGTAGTCTGCAAGTTTCGATGTGGGTTCTTTTAGCTGGTCTGGGGTTTTGTTGCTCTGAGTATGTTGAGCTGCATGCTCGTGGCGGTCTTCACGGCTCCATTTGTTCGGAATCTGTTGTGGAAGTGTCTCGGTCATCTGTGGAACTGTGGAAACCTGGTAAGATTTGTTTATCTGCTTGTGTCTAAACTGTTCTTGAGTTTTCTGTCGTCTTCTCGCAGCCGTA SEQ ID NO:277 Late LPZ-257GCGGACGCCTGCTGTTGAAGAAGGATGAAGTCATTGTCTGCGGCCCTGTTCAGCATGATTTCGGCATTCTTAATCTGGTCAACCAGTCAGAAGGTGGCGCTGAAGGTGACGAAGAGGCAACCTGGGTAGCTGCACTGGAAACTCAAGCTGCAAGGGGCACCGACCCTCAGACTTCGCGCGATAACTTCTCCCTCTGGGTAAGTCGATGCCAAGGTCCTTGTTCTGGGTTCTTCTCTCTGTTTCGCATGTTGTTCTTCTCTCTGTTTCATTTGTTTTTCTTCTGTCGTCTCTCGC SEQ ID NO:278 Late LPZ-258GCGGACGCCTGCACATACAAAGAACGACAAAAACAAAAGCATAAAATCCAATAGATGCAACTATATATCAAGTCAGAAATGATATAACTCATCATTATTACAAAGAACAATAAGAGTGGAACCATAATAATAGTCGTCTATTATTGATAAATAAAGAAGAATACAACCATAGTTCTGTCGTCTTCTCGCAGCCGTA SEQ ID NO:279 Late LPZ-260GCGGACGCCTGTATAACATGCACCAAGAGACCCAATCAAAGCACATGCAATCTGTATATATAGCAGAATAACAGCCAGGGATTGCACTCTATCGTAATCGCGAAACCACGCACTAATATGTGCCCATGCTGATGATGCACACAGCATGTTCTGTCGTCTTCTCGCAGC CGTA SEQ IDNO:280 Late LPZ-261GCGGACGCCTGAACTGTATAGAGTTGAAACTTGAGGGAAGGCTTGCTGCCACCAAAGCCTCCCTCCTCTTTCCTTGGCGGTTCGTCACCTCCTTTCGCGTCAGAGCCCCAATTCCCCTCCTGCGCACACCAGCAAACTGCATCGAATGTTTTTTCCACCATTCTGTAAATTCCCTCGGAGTTACCTTGGGGCAGAAGCCGCATTGAAGAGCATTGAATGCTATTCATTATCCCACCGTAAACTACCATTGCAACCTGCCTGTGTATCGACCCGCTGTCCTCTACGCGTGGCTGGCACATGGCGTCGTTAATTGCATGTTGACACCCGTATCCGGGTGTGCTTGTGTGCTCGTCTGCATATCATGTTTTAGGATCTCATAGAAGGTGGACCA TTCTGTCGTCTTCTSEQ ID NO:281 Late LPZ-264GCGGACGCCTCTTACAATGTCTCTAAAGATTGGAAAGATTGTCTTGTCTGCAACCATAACTTCCGCGTGCTTTCTTATTAATGCAACCCACTGTGATCCTTTCCGCCATTTATCCTTTCGAATGGTTGGAGCCATTTTTGGGTTGTACCGACTAGCTTTTGGGTCTACAAAGCTGTCTACAAAACTCTTTGGAGATGACATTACATAATCATATGTATAGCTGAAGTTGTACAAAGGTACACAACTATCTGAAACCAAAATGAATCTCTCGTTAGCTGGATCCTCGAGTGCTTTCCTAAGTAGAATACGCTCCGCTTCTATCATACTGGCTTCTCCCCAAGTACCTGTATGCTATCACTAAGCTGCCAGCCGTAACAAAATGTACATTCTGTCGTCTTCTCGCAGCCGTA SEQ ID NO:282 E,M LPZ-265GCGGACGCCTTGCTAGGAGAGCTCTACGCCATTATTTGAACGATTGAGCCGAAGTTTCACCGTTTAAGGCATTTGTGTCCCAGAGGTTATTGGAGATTAGCAGCTTGGATTTGGCTGCTTCGCTCAGCGCCGTGATTCAGCTTTTGATTGATTCTCTCCAGTTTCATCCTGTAACGACAATGGCAATGAAGACCTACACATTGCAGTGGCAGCTGCGTACGCTGTAGTCCTGATGTTCGCTCTCTTTGGCATCGCAAAGGCTGCTGATGCACCGTCTCCCAGCCCCGTTACTGGCGCGGGTTCCATGGACTTCGTTCCTTCTGTCGTCTTCTCG CAGCCGTA SEQ IDNO:283 Middle LPZ-266GCGGACGCCTTATCAGCTGGGGGCATTCATAGGTATGGAAATTCAGATCAACTTCAGTGGACAGTATGTGGATTTAGGCGACCTGTGACAGTTCACGATATCTATTCATTTCTATCCAGAGACAGATTCCCATACTCACCTCCGTCCTTCCCATATATTTTCTGGAAGGCATCATGTCCTCCCAAATTTACTCATTTTGCCTGGCCGTCGTTTTACAA SEQ ID NO:284 LateLPZ-268 GCGGACGCCTGTTGCCACAGAAGAATGAATAATGCTTCAAATTTGAGACCTCTTCGGAGGAAAATCCTTGTTCTTACTGCCTAACCACTCATGATGATCTGCGTCACGCTGATTATGAGCTGCAATTTAAATTATTTCAGATGAAACATTCCCATATTGAGCTTGCAGCAAGTTGCAGACCCTTCAATTTCAGTTCTGTCGTCTTCTCGCAGCCGTA SEQ ID NO:285 MiddleLPZ-269 GACGTTGTAAAACGACGGCCAGGATTAAGGTTCATGAGCTCCGCAACAAGAGC TCAG SEQID NO:286 Late LPZ-270GCGGACGCCTCTAGGAGCCGGCGGAATTCCTGTGAGCTCGAATTTGCCGAGCAGGTTATTGTCCTTCGTCCGCGCTCGCTCACCTTCATATACTTGAATTAGAACCCCAGGCTGATTATCTGAGTAAGTTGAGAAAATCTGCTCCTTCTTGGTTGGAATGGTGGTGTTCCTCGGTATTAATACTGTCATTACACCTCCCGCTGTCTCCAACCCCAGACTTAATGGCGTGACATCTAGCAACAGCAGGTCCTGCACCTCTCGTTGCCTTCGCCGCTGAGAATGGCAGCCTGCACAGCTGCACCATATGCCACGGCTTCGTCTGGGTAATGCTCTTACAAAGCTCTTTGCCATTGAAGAAATCTTGGAGCAATTGTTGTACTTTGGGGATACGAGTCGAACCCCCGACCAAGACGACATCATCTATTTGGCTCTTGTCCATCTTAGCATCTTCGCATACATTTCTCCACAGGCTCCATACTTCTCCTGAAAAGATCCATGTTGAGTTCCTCGAAGCGAGCTCGCGTAATTTGTGGCGTAAAAATCAATTCCTTCATATAGAGAATCAATCTCAATCGTTGTCTGTGTAGTAGAAGACAGCGTTCTTTTGCCCTCTCACATGCTGTTCTCAGCCTGCGAAGAGCTCTGGCATTCCCGCTGATGTCTTTTCTGTGCTTTCTTTTGAATTCCTGCACAAAGTGATTCACCATTCTGTCGTCTTCTCGCAGCC GTA SE Q IDNO:287 Late LPZ-271TAGCCATCGCCATTCTATAATCTTAGGATCCTTGCTGAACGATAAGCCCATAAAATTGATGCACTGCCTCGCTATCCCTGGCCGTCGTTTTACAACGTC SEQ ID NO:288 Middle LPZ-272GACGTTGTAAAACGACGGCCAGGAAATTACAGCTACCTCTAACTGGTTTGACGGCGTTGCATCTATGAGCCGCAAGGGTTCGAATCCTCTGCGGGCCAGATCTGCGATGGAACCCTGGGCGAGTGCAATGATGATGAAGAAGAGTTTGCGATGGATTCTGAAGCGCACGGGAGGCTTCTGAGGAGGATCCGTTACTATATCAGCTACGGAGCATTGGCTGCTAATCGCGTTCCTTGCCGACCTCGGTCTGGGAGGTCTTATTACACTCGGAATTGTTACGGCGCAACAGGCCCCGTCAGACCTTACCACAGAAGCTGCACTGCTATCACTC GTTGCAGGCGTCCGCSEQ I D NO:289 Middle LPZ-273GCGGACGCCTGGGAAGCAATGGATGGGTGGCTAGACGCCATCCGTCTGTGTATACTATTTTTGCACGCGGAAAGAGTGATGTCCTGGCCGTCGTTTTACAACGTC SEQ ID NO:290 LateLPZ-274 GACGTTGTAAAACGACGGCCAGATTCAAAAGAAAAAATCCTCACTTCTTGGCTCCGTTTGCGCTCCCGCCGAAGCTCCTCTGCAACCCCTCTGCAGCGTACACTGCATCCCGCTCGCGGTGCTGGCTCACCTCGCAGGTCCGCTGACGGTAAATGGTTTCCAATAAAGCTATTTGTCCTCTACCCAAAATCCATCTAGCATTCGTTGTGGATTGACATTCTGCCATTTCTCTGCTTTTCTGGTTGATATGCAAAGATTGAAAGCCCAATTGCAAGCAGTGGTCGTGGATTCACTATAAGGCGTCCGC SEQ ID NO:291 Late LPZ-275GACGTTGTAAACGACGGCCAGGAATAAAACAAAGCATCACTGCAAAATTTCAAACGTGGTAATAACGGCTAGCCAGCTCGACGTGAAGGCAGTGGGGGCCTTGAGGTTGCCTTTTGGCGTTCAAAATTGGCTAGACTACCATAACATAAATATTGATTTCTCAGTGACATCACTGGTTTGGAGTCATCCACAGCCTGTGCACCAGTACGGCAATTGCCTTTTACATGAAGCCATCCTTTCACTTTTACTTTTGAGATTCTCAGAACTGAGGGGCTAGGC GTCCGC SEQ IDNO:292 Middle LPZ-276GACGTTGTAAAACGACGGCCAGCACCTTCCTAGTCCCCTGTCCATTCTCCTGAAATAGGAGCAGTTTGACCCAGTCCAGTTTTCAGAATTGAGAATATGAAACAAAGAACCAAGCATATGAGAGAACATACAAAGACTTTGTATAAACTACTTTTCACAGGATCTCAACAGCCCTCTGCTGAGATCCATTTGATACAAGGCCCCTTGCATCTCCACCCTCTCCCTTATCACCTCCACTAGAAAGATGATGGAAAGCAGACACATGGAAATGTTGCTGCAG GCGTCCGC SEQ IDNO:293 Middle LPZ-277GACGTTGTAAAACGACGGCCAGTTAGGTTGTATATTGATTGATGACTCTTTGACTCCATTTATGAAAACATCTTTGTTCTCGAGATTTAATCAGTATTAAGCTTTCAGAGTGAAGTTCAGTTTGATCTGCATAAACCTGATCCACCATATCTACATCACATCTAAAATTACTAAAATGTGAGGAGATGGAATTTGTTTCTTGAGAATCCCTATTCCTCATCGACACTGTTTACTGGATCAGATCCAATCAAACTCTTGAGAAGTAATCTCTGGAAAGAAATTAAAAAGTCTTTACCTGAATTATCTCGATATCAGAAGCAGAAATTATGATACATAGACTTCTTAATAATGAAGAGTCATTTTGCCAACGTTGTCTTTGCCACCCCACCAATCCCCATGATCCCAAAGATCTGAGGTTTCCATCTCTATGTGGCTGTGATAACACTGGATTTTTCAAAAATCTTCTACTTTCGCATCCAAACCTTTTTGGGATATTT SEQ ID NO:294 Late LPZ-278GACGTTGTAAAACGACGGCCAGGGGGATGGGAGATACAGAAAGATTCCGGATAAAAGGGAGCAATGAACGGCTGGTTAAAGCGTAGTCCACCACACTAGCCCCACCTCCATGAGGCCTACACGTGAAGAAGCAGGATCTGGGAAGCGCGAGAGGCCGTCAAGATTATCAGCTCATGTGATTCGCCCAACTGCAAAAGATGTCTACCGTAGGCTGTGATGGGGCCCAAGGCGTCCGC SEQ ID NO:295 Late LPZ-279GCGGACGCCTATCAGATGGGTGAGTTGACCGACATTTATCGTCCGATAAATGTTTGAGGCTGATGTCATGGCAATCCACGTGTCTGCACCATATTTCATCGGAGCCCCTCGTCGGAATATTCCATCGCCGGAGAGCTGGCGCGATAGGTTTCAGGCGGCCGGTTTCTGGTTTGCAGCTGTGGCTTCCCGCGCGCCTTAACTGTTGGCCCGCGCGCACAGGGGAAATTACAAATTTCAACATATCCAATACCATCATATAACCCAACAACACTAGCAACAGATCCTGTTCTGTGCCATCGTCCAACTCTGA SEQ ID NO:296 Late LPZ-280GCGGACGCCTTAATTCGACTACAAAGATACTGAAGCCAATGATGACAGGTTGTGCCACTTTCCCAGCTGATAAAGACAGCTCTGAAATTGATAGAGCCAGAACTCCAGCTGCAATGCTCCCCAGAGCCTGGTTGAAGCGCTTGCTAAAGGTGGCACTTTATAGACCGACCCAAAACCTCCCTGGCCGTCGTTTTACAACGTC SEQ ID NO:297 Early LPZ-281GCGGACGCCTACTGGAAACCCGGTCCACCGAAGGCTGAAATTGTCCTGCTTTGTATACCGAATGGCAGGAAGGTTGTCGAGCATCAGGTTCACCTGGTAAAGATTATCGATCCTATGCTTCAATACCTTCAGCTGCTCTGCCCCAAGGACAGTAGTATTGCACAGGTAAATTCAGATTCATTGACATTCATCCGGAAGCGATATGGTGAGTTCTCGATCCTGTCCCCCATGAGGAGCTCCCCAAGATTTTCTGCCATGTCCTTCACACCATCCAAGGGCTTGCAGAAGGGCAGGCTGTAATAGCTGTAGGGAAGCTCTGTCTCGACTGAGGTAAGGGAATTGACGTTCACCCATAAATCTGACCCCTGGGAGAATATGATGTGAGGAATACAGTGCCCAGTAAATATAACTCCGCATTATACGTTTGTGTGTGCCTTCCCCAATATTGCCCCAACATAATCAAAACCCACAATCCCAAATCCTGGACCGTCGTTTTTACAACTG TC SEQ IDNO:298 Early LPZ-282GCGGACGCCTTGTCAGGACCAAATGTGTAAGAAACACCTCTGTCATTCGAGCCCCTCCTTGAATTGCATTGCAGGGGTCTGACCAAAGAAGATCACATTAACAACCCTGTATCTGGCACATCTGTAGGTCGAGGTATATTCTTTATTTGTTCCAAATTGGTCAGTTCAGGCGAAAGACCACCATGCATGCATAGGATCTTTTCATCTATAAGTGCAGCAACAGGCAGGCAGTTGAAACAGTCTGTAAAAAGTTTCCATAGTCTTACATTGAATCTGCGCTTGCACTCATCATAGAAACCATATATGCGATTTATTGAGGCACATTCATGATTTCCCCTCAGAAGGAAAAAGTTCTCTGGGTATTTAATTTTGTAAGCAAGGAGGAGGCATATTGTCTCTAGGCTTTGTTTGCCCCGGTCCACATAATCTCCCAAGAAATAAGTAATTTGATTCTGGTGGGAAGCCACCATATTCAAAAAGCCTTAGACAGATCAGAATACCGGCCTGT CGTTTTACAACGTCSEQ ID NO:299 Early LPZ-283GACGTTGTAAAACGACGGCCAGGAGACGGGAATACCTATTTTTGGGAGGATTATTGGGCTCGGGAATCAGCATATTGATGTGGCTGCAACTCGCATCCTCGATCTTTGGTGGTTCTTCGGCGATTTACACATTTGAGATCTACTTCGGTCTGCTAGTTTTCCTTGGGTATATTATATTTGACACACAGATGATCATCGAGAAAGCGGACCATGGAGACTATGATTATTTAAAACATTCACTGGACCTCTTTATTGACTTCGTTGCTGTATTTGTTCGCCTGATGGTCATAATGGCAAAGAATGCAGACAGTAAATCCAGGGAAGGGAAAAAGAAGAGAAGGGCTTGAACTATGTGAGATACAAAAATATCGAGAATAGAAGGGCTTGAACTAGGGCTTGAAAGCGTCCGC SEQ ID NO:300 Middle LPZ-284GCGGACGCCTATCAGACAAGGGTTGTTGACCGAACTTTATCCTCTGAAAAGTGCTTGAAGCTGATGTCATGGCAATCCACGTGTCTGCACCATATTTCATCGGAGCCCCTCACACGGAAACAACCTTAAGCCAAAAGGTGGTGCGATGACTTACCGGCCGTTTATGGTTTGCTTCGGTGGTTTTCTGTTGGGTGGTTTCCCGCGCGCGTTAACTGCTGGCCGT CGTTTTACAACGTCSEQ ID NO:301 Late LPZ-286GACGTTGTAAAACGACGGCCAAGAGGGGGAAACTCCCAAAACACTTTTCCATTTTTCTTCTTTTATTAAACTTCAAAGTATTTTCCAACAGAGTTACAAGGGGCCAACCATGTCCAAATCCATGCATTTACCAAGTACAAAGAATGGTAGTCCTTGGCTTGACCTATCGACTAGCCAAAAGTGCCAAGTCCACAACTAGGGTGTGCCCAACCTAAGGTGACACCTTGCCTAGAAAAAACCCCAAACTTGGCACCACAAATAACACAGAAACACAACTCTTGACCTCTGCCAGAAACCAGGCTCTCTTGGGAAAGCCACACCTCTCTCTGTGATATGTCTTATCTCCAATTTCCCTTTTTGTGATGCACTCCCTTGCTTGTGGTTCTGCGATATCACACAAACTTACATTTCTGCGATTTTTGTTTCTTGCTTCTCCAAATCATGCGATCTTATTTTTAACCCTTGAGACCCTTCACACTTTCCATCCATGACGTCACTTCATCGTTTTAGCCAATTCGTCATTTGGGCATGTTGGGCGTTGGGTCTACCCGTATTCCGGTCGTACAGGCCAAATTGACCATTTTGGTCCAGGTGGGTGCACCCATTCCTGGAGGGCGTTC GGC SEQ IDNO:302 Late LPZ-287GCGGACGCCTCCACAGAGCTCACACATACAATATACTATGATGCCTCCAGAACTATGGCACTCTGTATGCCGCTTCAATATGGATTAGCCCACACTGCGCCATCCAATTAGGCGAATCAACCTTATAGCACCATCCACAACCTCCAGCGCTCTCTTTTTCACGCTAGATTGGCCAACTACAGGCTTTACAACACTACTCATATACAACTCAACTCGGCTCCTCTGCTCACCACTAAATCACACAGGCTCCAATCGCTAGACAGAGCCACTACACAGGCACTAATAGCCACTACACAGGCACTAATCTTGGCGTCCTCCACCAGGTTCCAACAACAACCCCAAATTGCATATGCACTCCACAGTGAGCACCAACTAGGTCCACACAATAGGCCACACCAACAACACTCCAAGGACCCTAGATCCTGCCTCACCCAGACACCACTAGGCCTTCCTCACAGCTCACCTAAGTGAGCCAACAACTGGCTGGGCACACAGCTCCCAACTATATGAGCACACAGCCCAACTACAGCTCCAGCACACGCACAGCTACACGCACAATGCCTTCTCAAGTTCACAGCCACACCATAACGCAGCACAGTTCTTACAAACATATCTCTC CAGGCGTCCGCSEQ ID NO:303 Middle LPZ-288GACGTTGTAAAACGACGGCCAGGATAATGGACACGAGAAACCTTTGGATGTGCCTCTAAAGTGCGGGCAATCCTTAAAGCTGTTGAATTTGTGCTGTACACGAAGGTGCAGGGTCTTTATGCCACGAAGAATCAAGTACGCTGCATTTGGACTTAATACACCTCCCAAGACATTGTGCAAAGCACGTACTGTGCCAATAACCTTGTTTGAACCACTCAAACTGCCTGCAAGAACATCATTATGACCTGCAATATATTTAGTTACCGAATGCAATACAATATCTGCGCCGAGTGCTAACGCTTTCTGGTTAACAGGCGTCCGC SEQ ID NO:304 Middle LPZ-289GACGTTGTAAAACGACGGCCAGTCATTATTGACAATAATCCTTTCAGCTTTTTACTGCAACCTTTAAACGGTATACCTTGCGTTTCTTTCACTGGAGCACACTCAGATGATAATCAGCTTTTACAGGTGCTCTTACCTCTGTTGAAGCATCTTGCCACTCAGGAGGACGTGCGCCCTGTGTTGTATGAAAGATTTTACATGCCCGCATGGTTTGAAAAGCGTGGCATTCCAGCATCTGAGTGGCCCTTGTGACTTGGTTTTGATTTTGGATACTCTTTGTCATTTTGGGTCAAGGTAAAGGTGTACGTATCCAAGTGATGCAAGCGTCCGC SEQ ID NO:305 MiddleLPZ-290 GCGGACGCCTGATAGCACGAGTCTTCTGGGACGCAAATCAAGAGGCAGGTACTTCTTTTTCTTGTATGCTTCTCTTAATGCGGATCGCTGGCTCTGAGAAATCACAGTCAGAACCTGAGCTATTGATAGCCTCACGACCTTGATTTTAGAGAGTTTGTTGGGCGCTCCTCCAGTGACCTTGCAACTCTGAGCAAGGCAAGCTCAGCCTGAGCTCCTTGACCTGGCTTAACAGCTCGGATTTGCCCTTGTGGCGGACTCAAGGACCTTTAACCTGGG CGTTCGT SEQ IDNO:306 Late LPZ-293GCGGACGCCTGGTGTCGCTGGGCCAGTTCAAGTATTTTAGCAACAGTGTTCACACTTATTCCCTGTGATATTCTTGACTCACACAACCACCTAACTGACGCAGACCATATCGATCTGCTGCTGTAAGCAAATGTTCGATCATTGTCTCAGGTGTCAAAAAGCAAGGGGATGGATCAGAAAGCTCTTCTAAATCTGCATGCTCCTCTAAATCTGGAAGGGTATCTTTGTAAATAAAGTGTAACATAGCCTTAAACACCTCTGGCCGTCGTT SEQ ID NO:307 Late LPZ-294GACGTTGTAAAACGACGGCCAGAGGTGTTTAAGGCTATGTTACACTTTATTTACAAAGATACCCTTCCAGATTTAAAGGAGCATGCAAATTTAAGAAAAACTTTCCTGATTCAACCCCCTGCCTTTTGGCACCCTGAAGATGGTTCAACAATTTGCTAACGGAACCAATTCAAAAGGGCCGCCTCCATTTAAGGTGTTGTGTTAGTCCAGAATATCACAAGGAATAAGTGTTAACACCGGTGCCAAAATACCTGAACTGGACCAACGACACCAAGCGTTCGC C SEQ ID NO:308Middle LPZ-295 GCGGACGCCTTGTAATCCAGGGCCTTGAATATTGTAAGAGAAGATCGAGAAATAATAGTTTTCTTATTATCAGGAATCACAGCTTGAAGAAGGCAGACCATGGACTCCCACTGGCTTCGTGATATTGAGTCCCCAACAAACATTAGTCGTTTTCCCCTCAATCTCCACAGCAAGTCTCTGGCATTGAATCTGCGAAAGGAACACCCGAGTGGCTTCCACCTCCATTTCTCGTAATCAGAATCTGGCCGTCGTTTAACAA SEQ ID NO:309 Late LPZ-297GACGTTGTAAAACGACGGCCAGCAGAAGACCAGTGCAGTATGCTGCAGCATAGTTTGTAAGCCCTACTTCGAGTCCATAACGAGGCAACTCCCTAGAATAAGCAGCCGACATAACAACATCTCCCGCAAGAGTTGCATAAATGATCTGTGCCACCACATCCTTGTTGTGAATCTAACGACCAATCGGTATTTGGGTGTGTTGTACTTGTTCTTATCTTGGTTAA TCAGGCGTCCGCSEQ ID NO:310 Late LPZ-299GACGTTGTAAAACGACGGCCAGCATCCATTGCAGAAATTTTGGGGGCTATATTTAGCAACAGATATCACAGCTGTAAGTCAAAGTTGGACCCTTCTTCTTCGACATCTTTTCCAGCTGTGCAATAAACTGAACACTGTCCTTTTGGATAAGCTCCTCAACATATTTAGAAAGTTCAACATCCAAGACATTGCGGTACTCCTCAACATATATGGATGCAAGTTCATCATCTGCAGCTGGTCTCACCGCTGTACAAACTGTTTAACATGGTTGACAGTTGCAAGTTGAGCAGTCCGTGGATCCAAATAATGAGTTCCGTCAAGCTCACTGAACTCAGTCACAATCACCTGGCCACTTTGATTGGGCATCTCGAGGGATATCATGTGAGACTTGTTGTGGATGGGGAAAGCGTCCGC SEQ ID NO:311 Early LPZ-300GCGGACGCCTGCATAAACATCGCTACCCTGGGGATGATTAATAATAGTACCAGGGTTAGGATTTTCTTCATCTTGAGCGATATCATCATACATAAAGACCACAATGTTTTCCTCTTTCAAACCGCCTTTCCTCAGAATTTGGTAGGCATGGCAGATATCAGCCTGATGCCTGTAGTTCCAATAACCGGAAGAACCAGCCAACAGAATAGCCCACTGAGTACCGATCGTATCACTATCATCAACGATATGATCGGTGGGCATTTTCAGTACTGAATCCCAACCCCTTCTGGCCGTCGTTTTACAACGTC SEQ ID NO:312 Middle LPZ-301GCGGACGCCTAGACTGGGCATACCAACTACCTTCCTCATGCCAGGCCATGGGCCACCTACCTGGTACTTAGGCATAACACCTTACTTACGAGCATGCCAGGCTCAGTCAGATAGGCATGCATCCCACCCACCTAGCTATGACCCAATCCTTATAAACACTAGATATTCTCCCTGGCCGTCGTT SEQ ID NO:313 Late LPZ-303GCGGACGCCTAGACAATCATACTGAAGATCTGTAAGCCATGACAAGACGAATAAAACGAAGCACGGCGCAACCAGCGTGAATATTGACGCCTTAATTTCATTCAACTGGGTTGCGGATTCTTTATTCCTCAACAAGTGTTCGATAGCTTCACATACGCAAGGCCCCTTTTACTCTCACCTTCATGGTTTAATGCTGTAACCGTCGAAGGTTGATGAAAGGACTTGGATGATGATGTTGCCAAAAAAAAAAAAA SEQ ID NO:314 Middle LPZ-304GCGGACGCCTGCTCAACACCTGTATAGTCATTTCTTGTTTCCTTTTCTCAATTTTCTCTTTCGAATGACCGCATTGAAATTCAGGCTGCCCAACGCGTTTTTGTTTTCACAATTAATTTTTGAATCATACGCGAAGATCATGATGAGAATGGTTGTGGAAAAAAACTGTT TGTAAATATTTAGSEQ ID NO:315 Middle LPZ-306ATATCACATTACCATTCAAAAAATAAACATTTTACAAAATACAATTCCATAACAATTTTCTTCCCTGTTCCAACCTCCACAAAAGTAAATGATCGTATAAGAAATTAACTACCAACAAAAATCCCAAAGTTAAAGGAAGACATCCCCAAAAAAGATGTAACTTTCAAAACCGGATGACTTCACTCCTGCCATTGCACCTAGTCATTTACTTCTCAGAGGAGTTTGGCCCTTTCTTCTTTCCAAAAGTAACCACTGCGGTAAGAAACCGGCGGTTGTATTGCATTCGCTTGTAGGCGCGGCCTCTAGGCTTCTTCTTCTGTCTTGTTTGGCCACCTTAGGGTC CGC SEQ IDNO:316 Middle LPZ-307GCGGACGCCTTGGTACAATGGACTTGCAAAAATAAAATGAGTTCTCATTGTGGGTGAGATGCGGATATTTTATGCATAGGCACTTCATGGAGATGTGGTTATAAACGCCATCTTAATATCTGTACCTATTACTTTCAAAATATGAAGGCAAGATGGAAAGCTACTCATCTGTTGTGAAGTCAGAATGTTGGTAGCGGTTGGGCTCTGAAAGTAAGAAACTTTTTGATTGGTTTAATTAAATGAGGGAATTTGCCTGGTTTCCCTCTTCCTTCCGAAAAAAA ATTTATTTA SEQID NO:317 Late LPZ-308GACGTTGTAAAACGACGGCCAGACAATATTGGAAGGGAGAAAGGCGCCAGCAGGGTTGAGGGGAAGAAATGCATAATGACATATATAATGAGATCTATTTGTATACGATATTACGGGTACGATCGATGATTCGAGCTACGATCCCATACGACGCTAAAGCGTAATTACATATATAATAGATGCATTTCAGAATGACTTATCTATTTCATTACGCGATATTATATACGTAATTACGTATATAATTGCAGAGATCTCACCGACCAACCAAATAGTCTTTCATTTCATCCCAGGCGTCCGC SEQ ID NO:318 Late LPZ-309GCGGACGCCTGTATCACTAGAGGTGAATACTCAGCAAGCAAAACTGAAGGATATTATTGAAAAAGCTGTCAAGGCTAAATTGGGTGTCAATTCCCCATTGATCATGCATGGTTCTACACTTTTGTTTGAGTCCGGTGATGACATGAGGAAGATGTTGCTGCACATTATGCACAAAACTTAGAGAAGACGTTAGCAGAATTTCCAGTTCCAATCACAAATGGTGTTATTCTTACAGTAGAGGACTACCAGCAAGAGTTCTTATGCAGTATTAATATTAAGCACAGAGATGACTTTGATGAGGAGTCAGGTGGCATTGTACTGTCTGGAGGCGTCCGC SEQ ID NO:319Late LPZ-310 GCGGACGCCTCCTTGTAGATACGATACATGAGTCTAAGATCAAAATCATACAAGAAGAGCTTCATTTCCGGGCCTCACCTTTTCTACAAGCTCCTTTTTGGCTGGTGGAAAGCCAAACACTCTGTATCGGAAACACTCCTGCCTAGTTTCAGAATTACACATAAAAATCAAGCCGGCAAACCTATCTTTGCCACTGCCATCTCATTGTTTGCGTCCTGGCCGTCG TTTTACAACGTC SEQID NO:320 Late LPZ-311GCGGACGCCTTACTAAAACGACGGCCAGATGTGTAATGGGGAAAATGTGTCATGATAGTTGGGTACAAATAACGAGCCACCTGCTCTATGTTTTCGAAGTTTTCTGTTGGATTTGTCCGGGTGAGAGAGCGTTCGTTCGTTGCGCGAGAGGGGCAAAATGCTGAGCGTGGGGAATTGCCATTGCCGCCCCTGGAAGTGCCGCACGAACGCGATCACATTTAAATCACCATTTACTTCATCATCACCATGGTTAAATGCAGTCCCTGCTCCTCAAACAGGACTTCAGATCCTTCAAGCTCGAAATCTCCGCCTCTGCTTCCTCGAAGACAAGACTCTGTGAGGAGGAAGCGCAGCAGCTGAGCTTAGCGGATCTGCTGAAGCCCGGTGGCCTCGCCCCCGATGGGTTCTCGTACAAGGAGAACTTTACCATACGCTGCTATGAAGTCCGAGTTAAACCGCACTGCCACCATTGAGGCGTCCGC SEQ ID NO:321 Middle LPZ-312GACGTTGTAAAACGACGGCCAGCAACCAAATAAACCCCACATGTGCTCAATGTTTTAGTATAAAAGGAGATGACTTAAGAGTCATTTCACACACACTTCTATCTTGATTTCTCCCACTTGTCTTGGGTTTTAGTGGAAGAGAAATCTAGGAGTGGAAGCCCTAGACGTTGGAGGATAAGAAGGCAACCCTAGAAGGCAGAGCTAACGCTATCCTAAGGCAACCCTAACGCTATCCTAAGGCGTCCGC SEQ ID NO:322 Late LPZ-314GCGGACGCCTGCTCAGCACCTGTTATAGTCATTTCTTTTTTCCTTTTTCTCATTTTTGTCTTTCGAATGACCGCAATGAAATTCAGGCTGCCCAACGCGTTTTGTTTTCACAATTAATTTTTGAATCATACGCGAAGATCATGATGAGAATGGTTGTGGAAAAAAACTGTTTGTAAATATTTAGGTGACCAACAATTTTCATGATTGCAATCTAAAGTTGATAATTGATTTATCGGGTCGACATTTGTAATTATTAACACGGAAAATCTGAGGCTTACAATTTTTGGATTGTAAATATTTAGGTGACGAACAATTTTCATGATTGCAATCTAAAGTTGACAATTGAGTTATCGTGTCGACATTTGTAATTATTAACACACAAAATCTATGAGGCGTCCGC SEQ ID NO:323Late LPZ-315 GCGGACGCCTCATCAATCCATGGTTGTACACGCGCCTTCAAAGCGGCTCCTTATGTCGCGCAGCGTCTACTTGTTCCTTGAGCGCTTTTCCCTGCTACATCCGCGCGAGCCTCTGTGCAAGGGCCACTGTCTGCGCGGTCCCTTTAACTTCGTCGTACTTCTGCTGCAGCTCACGTGTCTCTATTTCTAAGTGCTATATATTTGGGTCCTCCTGCATAGTAGTGAACTTCGAACGACTCCTCAAATAGCCAGGTGTAGTCTTTCATTGCACTATTGATCTCCACTATTCCTGCTATAATGGCGCTAACATGCTGTTCCTTCACCTTTGGCGGAGTGAAGGCTGCGCCTCTGGAGCTCGGTTATTTGAAGCTGAACCTTGGGCATATCTTCCTTCACCTCGTGCATCCCCTGCTTCGAGTTTCTGGATGCACGCCTCCACTGGGTCTTCTGCTGGGATGGGCAACTCTAAGACCAACTGGTATGCGTCGC SEQ ID NO:324 Middle LPZ-318GCGGACGCCTTCTTCAATCCATCAGGCCTGATTAATGTATTGACCTTCTTTGTCTGAATGTCATACATTTTTTTCACTGCATCCTTGATCTTCTTCTTGTCTTGCTTTCTATCCTTTCTCTTGCTTTCTATCCTTTCTCTGGC SEQ ID NO:325 Late LPZ-320GACGTTGTAAAACGACGGCCAGCAAAATTGATATAAAGAATAGACACATCGACTCAAATGAAGTGACTCAACAGTTCATAATTCATGTCAGCTTGAATGCATGGACATACACCCATAAATAGGCAGTTGGGGTCACCCAAAAGAACATAGAAACATCTCGCATCTCTCTGAAGAAACTCGGATGGGTACAGGTCTGTGACTTCGCATATTTTGAAGGAGCACTCTCTTGGATAAGTACAATATAGGTACCATCTCGGACTCGCCTGAAATCTCGCAAAGAAGTCTCATTCTCCTCCTTGTTACAGGCGTCCGC SEQ ID NO:326 Late LPZ-321GACGTTGTAAAACGACGGCCAGAAGCATCAATAAACAAAATGACAGATTAACAAGTTCTCTCTTAATCTTAAGAGAATACATCAACATCCAAGTAAAGTCATAACACATTTACAAAATGGTGCCACGGTATCCATTCTCTGTAACAAGGTTTTTCTGAAAATAGTTTTCCTCTTATCTATGTAACTCTTCATAGGGATGCCTGTGTCAACGTGCCATATTCCCAAATTTGGCCACAATCAAACCTTCCTCATTAGAAGAAACAATCTCTGGTCTAGCTCAAAATTGGCAAAATTTCCAGCATCTCCCTTTAACATCATTAGAAGGCGTCCGC SEQ ID NO:327 EarlyLPS-097 GGGAGATGCTAATTTGAAGCCCTTCTCTGAAGGTGGACAATTCCAGCAGCAGTGGTCTAAAGCCCCAATATGGCTATAGAAATTCTTCTGGGGGTTGCACCTATGGAAGAGGGTCGGAGAGGACGAAGCTGTGGATCGCTCTTACCATCTGTGCGGAAGGTGGTAGCAGAATTCATTGGAACGTTCTTCCTCATATTTGTAGGATGCGGATCTGTCGTTGTTGATAAGATAAGCAACGGTTCCATAACTCATCTTGGTGTGTCGCTTGTATGGGGAATGGCGGCCATGATTGTAATTTATTCCATAGGCCATATTTCTGGAGCTCATTTGAATCCTGCAGTGACGTTGGCCCTTGCGGCTGTGAAGAGATTTCCATGGGTTCAGGTTCCAGGCTACATAGTAGCTCAAGTATTTGGATCGATATCTGCTGGGTTTCTCCTACGTTTCATGTTTGGAGAAGTGGCATTCATGGGAGCCACAGTCCTTCAGGCTCAGAAATGCAGTCTTTCGCTTTGGAAATTATTACTACGTCATTGTTGGTGTTTGTGGTTTCTGCAGTCGCCACTGATACAAAAGCGGTGGGTGAATTGGGAGGTTCAGCAATTGGAGCGACCATCGCAATGAATGTAGGCATATCCGGACCAATCTCAGGAGCTTCAATGAATCCAGCAAGGACAATAGGATCCGCAGTGGCTGGCAACAAATATACAAGCATTTGGGTTTACATGGTTGGGCCTGTAATCGGTGCGCTAATGGGTGCAATGAGTTATAACATGATTAGAGAGACAAAAATGTCCGAAAGGGAGATTATGAAGAGTGGGTCATTTGTTAAGGACATGGGCTCCAGCGAATCAACAGCATAACAACTTAGAGATTTNTTGCATTCCCGAGACGGTATCCAGTGATAGTGGAGAGTAGTCATAATAAGATTTGTGAAAATGTTTGTGTAGATTAATGTGTAAAATTCAATCCATCAACCATGAAGCGAACTGCATTCCGTTTTTAAATGTTTATTGGATTTGAATTAATAAACAGCTTATACGTGAAAATCCCTACTTTATGTACGGA SEQ ID NO:328Early LPS-098 ACTATAGGGCACGCGTGGTCGACGGCCCGAGCTGGTATCCGATGAAGCTAGATTCAATGGTTCAAGTCCTATGAAAGCTAGATTGGAGAATTGCAAAGAAATCTAATCTCCGTTAGTTGTCCCAACCACTGACTCGCACCCAATCAGAGTATATTAAAGTTAAAGATTATATAAAGGTAAATTGAACATTTATAAAATCTTAAATGTATTTTTAGAGTTAAACATTATATAGAATATTTAATGTAGTATAGATATAATAAAATATTAAAAATTAATTTCTCTTTACTATCAAGTGAATAAAAATAAAAAATAAATGTAAGACAATATAATAAAAGACTTGTTTTTAGTGCATTTTTTGGACTCTTCGTTATTGTGTGGTATTGTGTTATTTAAACTGATCTTTTTACTGTATATATGGATGGGTTACCCATCAAACTTGTGATTTCAATAAATTCCTCCCGGATTTTAGAGAAATTAGACCATAAAAACTCACGAAAAAAATTTTAGACCATAAAAACTCACGAAAAAAACTTCCCCAAAATCACGCTAAAAACAACTAGATAAAAAAATACCCATCTTTGATGATGTGGATAGTGACAGCCTATTCCAAACTATCACCTAAATTGTAAGTTACATGCATAACACGATGACCTCATCTATACGTTGTGCCAAATAAAGGTATGACCGTTCAAACTAAAGAATCAACGAGCTCCAACGCATCTTTTGCTGTGGGGGGATTTCTCACGGCTTAACNTTCATGGANCCGATTACCTTNCTANCCAACCAAGGGTTTTAACCTGGCAAATNCCAAACCAATTACCAGCTTNACAAATCAACCGAGCCGCCCNACCGGGATCATTTTGGTCAAGTCTCGAAAACNGGCATTGGGTATATGGNATATGGAATTGGAATTGGATCAATGGTAACCTTGGGANAAGCTTAANTTGGAAANCCCTTTTTTTTGANGGGGGCCAANTTCCCGNNCCCCCGG SEQ ID NO:329 Early LPS-099ATACTCAAGCTATGCATCCAACGCGTTGGGAGCTCTCCCTATGGTCGACCTGCAGGCGGCCGCGAATTCACTAGTGATTAGATGGTAAGAGCGATCCACAGCTTCGTCCTCTCCGACCCTCTTCCATAGGTGCAACCCCCAGAAGAATTTCTATAGCCATATTGAGGCTTTAGACCACTGGTGCTGGAATTGTCCACCTTCAGAGAAGGGCTTCAAATTAGCATCTCCAAGTACATTGATCTATTCTATTCATATACATATAACAATGCTGCTCGAGACTGACAAAATGATCCGTTGGCGCTCGTTGATTGTTAGCTGTAATTGTTTGGATTGTTCAGTTAAAGCCTTGTTGGTAGGAGGTAATCGGTCATGAATGTTAGCCGTGAGAATCCTCACAGCAAAAGATGCGTTGGAGCTCGTTGATTCTTTAGTTTGAACGGTCATACCTTTATTTGGCACAACGTATAGATGAGGTCATCGTGTTATGCATGTAACTTACAATTTAGGTGATAGTTTGGAATAGGCTGTCACTATCCACATCATCAAAGATGGGTATTTTTTATCTAGTTGTTTTTAGCGTGATTTTGGGGAAGTTTTTTTCGTGAGTTTTTATGGTCTAAAATTTTTTTCGTGAGTTTTTATGGTCTAATTTCTCTAAAATCCGGGAGGAATTTATTGAAATCACAAGTTTGATGGGTAACCCATCCATATATACAGTAAAAAGATCAGTTTACCAGCCCGGGCCGTCGACCACGCGTGCCCTATAGTAATCGAATCCCGCGGCCGCCATGGCGGCCGGGAGCATGCGACGTCGGGCCCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGCGTTTACACGTCGTGACTGGGAAACCCTGCGTTACCACTTAATCGCTTGAGCACATCCCCTTTTCCAGTGNGTAAAACGAAAAGGCCCCNCCATCGCCTTTCAAAAATTGGCAACTGAANGGGAAGGACCCCCT SEQ ID NO:330 Early LPS-100ATACTCAAGCTATGCATCCAACGCGTTGGGAGCTCTCCCATATGGTCGACCTGCAGGCGGCCGCGAATTCACTAGTGATTAGATGGTAAGAGCGATCCACAGCTTCGTCCCCTCCGACCCTCTTCCATAGGTATAAAACCCAGAATTTGGTGAGCAGGAAGAATTTCCATAGCCATATTGAGGCTTTACACCACTGCTGCTCGAATTGTCCACCTTCAGAGAAGGGCTTCAAATTAGCATCTCCAAGTTACATGGATCTATTCTATTCATATATTTATAACAATGCTGCTTCGAGACTGACAAAATTATTTGTTGGCGCTTGTTCATCGTTAGCTGTAATGGTTTGGATTGTTCAGTGTAGGACCAGCCCGGGCCGTCGACCACGCGTGCCCTATAGTAATCGAATTCCCGCGGCCGCCATGGCGGCCGGGAGCATGCGACGTCGGGCCCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCTTCCTTTCTGGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTTCCTTTAGGGTTCCGATTTAATGCTTTACGGCACCCTCGACCCCAAAAAAACTTGATTAGGGGTGATGGGTCACGTAGTGGGCCATCGCCCTTGATAGACGGTTTTTCGCCCTTTGACGNTGGAAGTCCACGTTTNTTTAATAGNGGGACTCTTGGTTCAAAATGGGACAACACTTCAAACCTTTTTTGGGGNTATTTTTTTGATTATNAAGGGATTTTTGCCGNNTTTNGGGCCTTTTGG SEQ ID NO:331 Early LPS-101ACTATAGGGCACGCGTGGTCGACGGCCCCGGCTGGTTTCAATAAATTCCTCCCGGATTTTAGAGAAATTAGACCATAAAAACTCACGAAAAAAATTTTAGACCATAAAAACTCACGAAAAAAACTTCCCCAAAATCACGCTAAAAACAACTAGATAAAAAAATACCCATCTTTGATGATGTGGATAGTGACAGCCTATTCCAAACTATCACCTAAATTGTAAGTTACATGCATAACACGATGACCTCATCTATACGTTGTGCCAAATAAAGGTATGACCGTTCAAACTAAAGAATCAACGAGCTCCAACGCATCTTTTGCTGTGAGGATTCTCACGGCTAACATTCATGACCGATTACCTCCTACCAACAAGGCTTTAACTGAACAATCCAAACAATTACAGCTAACAATCAACGAGCGCCAACGGATCATTTTGTCAGTCTCGAAGCAGCATTGTATATGTATATGAATAGAATAGATCAATGTAACTTGGAGATGCTAATTGAAGCCCTTCTCTGAAGGTGGACAATTCCAGCACCAGTGGTCTAAAGCCTCAATATGGCTATAGAAATTCTTCTGGGGGTTGCACCTATGGAAGAGGGTCGGAGAGGACGAAGCTGTGGATGCTCTTACCATCT SEQ ID NO:332 Early LPS-102ATACTCAAGCTATGCATCCAACGCGTTGGGAGCTCTCCCATATGGTCGACCTGCAGGCGGCCGCGAATTCACTAGTGATTAGATGGTAAGAGCGATCCACAGCTTCGTCCTTCCGACCCTCTTCCATAGGTGCAACCCCCAGAAGAATTTCTATAGCCATATTGAGGCTTTAGACCACTGGTGCTGGAATGTCCACCTTCAGAGAAGGGCTTCAAATTAGCATCTCCAAGTTACATTGATCTATTCTATTCATATACATATAACAATGCTGCTTCGAGACTGACAAAATGATCCGTTGGCGCTCGTTGATTGTTAGCTGTAATTGTTTGGATTGTTCAGTTAAGGCCTTGTTGGTAGGAGGTAATCGGTCATGAATGTTAGCCGTGAGAATCCTCACAGCAAAAGATGCGTCGGAGCTCGTTGATTCTTTAGTTTGAACGGTCATACCTTTATTTGGCACAACGTATAGATGAGGTCATCGTGTTATGCATGTAACTTACAATTTAGGTGATAGTTTGGAATAGGCTGTCACTATCCACATCATCAAAGATGGGTATTTTTTTATCTAGTTGTTTTTAGCGTGATTTTGGGGAAGTTTTTTTCGTGAGTTTTTATGGTCTAAAATTTTTTCGTGAGTTTTTATGGTCTAATTTCTCTAAAATCCGGGAGGAATTTATTGAAATCACAAGTTTGATGGGTAACCCATCCATATATACAGTAAAAAGATCAGTTTAAATAACACAATACCACACAATAACGAAGAGTCCAAAAAATGCACTATTTACAAGTCTTTTATTATATTGGCTTACATTTATTTTTTACTTTTATTCACTTGGATAGTAAAAGAGAAATTAATTTTTAATATTTTATTATATCTATACTACATTAAATATTCTATATAATGTTAACTCTAAAAAACATTTAAGATTTATATATGGTCAATTACCCTTATATAATCTTTAACTTTAAATCCCTGATGGGGGCCAATAANGGTNGGGAAACTAACGGAAN SEQ ID NO:333 Early LPS-103ACTATAGGGCACGCGTGGTCGACGGCCCGGGCTGGTTTCAATAAATTCCTCCCGGATTTTAGAGAAATTAGACCATAAAAACTCACGAAAAAAATTTTAGACCATAAAAACTCACGAAAAAACTTCCCCAAAATCACGCTAAAAACAACTAGATAAAAAAATACCCATCTTTGATGATGTGGATAGTGACAGCCTATTCCAAACTATCACCTAAATTGTAAGTTACATGCATAACACGATGACCTCATCTATACGTTGTGCCAAATAAAGGTATGACCGTTCAAACTAAAGAATCAACGAGCTCCAACGCATCTTTTGCTGTGAGGATTCTCACGGCTAACATCATGACCGATTACCTCCTACCAACAAGGCTTTAACTGAACAATCCAAACAATTACAGCTAACAATCAACGGGCGCCAACGGATCATTTTGTCAGCCTCGAAGCAGCATTGTTATATGTATATGAATAGAATAGATCAATGTAACTTGGAGATGCTAATTTGAAGCCCTTCTCTGAAGGTGGACAATTCCAGCACCAGTGGTCTAAAGCCTCAATATGGCTATAGAAATTCTTCTGGGGGTGCACCTATGGAAGAGGGTCGGAGAGGACGAAGCTGTGGATCGCTCTTACCATCT SEQ ID NO:334 Early LPS-104ATACTCAAGCTATGCATCCAACGCGTTGGGAGCTCTCCCTATGGTCGACCTGCAGGCGGCCGCGAATTCACTAGTGATTAGATGGTAAGAGCGATCCACAGCTTCGTCCTCTCCGACCCTCTTCCATAGGTGCAACCCCCAGAAGAATTTCTATAGCCATATTGAGGCTTTAGACCACTGGTGCTGGAATTGTCCACCTTCAGAGAAGGGCTTCAAATTAGCATCTCCAAGTTACATTGATCTATTCTATTCATATACATATAACAATGCTGCTTCGAGACTGACAAAATGATCCGTTGGCGCTCGTTGATTGTTAGCTGTAATTGTTTGGATTGTTCAGTTAAGGCCTTGTTGGTAGGAGGTAATCGGTCATGAACTGTTAGCCGTGAGAATCCTCACAGCAAAAGATGCGTTGGAGCTCGTTGACTCTTTAGTTTGAACGGTCATACCTTTATTTGGCACAACGTATAGATGAGGTCATCGTGTTATGCATGTAACTTACAGTTTAGGTGATAGTTTGGAATAGGCTGTCACTATCCACATCATCAAAGATGGGTATTTTTTTATCTAGTTGTTTTTAGCGTGATTTTGGGGAAGTTTTTTTCGTGAGTTTTTATGGTCTAAAATTTTTTTCGTGAGTTTTTATGGTCTAATTCTCTAAAATCCGAGAGGAATTTATTGAAACCAGCCCGGGCCGTCGACCACGCGTGCCCTATAGTAATCGAATTCCCGCGGCCGCCATGGCGGCCGGGAGCATGCGACGTCGGGCCCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGCGTACCCACTTAATCGCCTTGGAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGGACCCGATCGGCCCTTTCCAACAAATTGCGCAACCCTGAATNGGGAAATGGGCCCCCCCCTNTTACCGGNGCAATTAAACCCCGGGGGGGNGNGGGGGTT CCCCCCCCCGTGGACCT

TABLE II Clone SE1-SE2 SE3 SE4 SE5 SE6 SE7 SE8 SE9 LPS001 0 249.4 1400.9827.6 1683.8 2019.4 189.2 4303.9 LPS003 701.2 555.9 2815.2 2445.1 3249.93094.7 227.1 3111.6 LPS004 466.1 335.5 2652 2701 2644 2329.6 218.52332.4 LPS006 753.1 332.7 3287.3 2964.5 2832.2 2688.9 182.1 1591.9LPS007 685.2 226 2010.2 1911.3 2600.4 1730.1 181.5 2737.7 LPS008 652.8274.8 2415 2219.3 2607.1 2294.9 155.7 1292.1 LPS010 558.3 356.1 2667.62881.1 2584.3 1573.4 161.7 1041 LPS011 3536.1 424.7 4021.5 3793.8 35903182 160.5 1471.7 LPS012 809 408.4 2206.7 2187.1 2282.2 2422.5 462.41483.2 LPS013 1211.1 391.6 2294.7 2652.6 2005.4 2167.8 166.8 1570.5LPS014 2191.9 432.5 2651.8 3013.5 3341.2 3586.7 178.8 3527.1 LPS0151197.9 306 5651.4 14828.6 20242.8 21558.2 1427.2 34472.3 LPS019 1830.2334.5 3329 3954.4 4347.5 4658.2 312.1 4743.1 LPS020 675.2 327.8 2258.32284.7 2542.7 2321.4 171.9 1609.8 LPS023 451.3 337.5 1401.9 1106.81766.2 1842.6 109.6 1365.2 LPS024 4585.8 444.5 3006.3 3431.1 3548.8 3759157.3 4062.3 LPS025 5102.3 397.1 4322.9 4699.6 5067 4973.2 262.4 5240.4LPS026 1568.7 285.9 1809.9 1830.4 2829.9 2381.7 164.9 1404.9 LPS0275499.9 458.4 4853.9 5218.6 2598.4 1756.6 457.9 2375.3 LPS028 4812.9314.9 2368.8 2616.5 3113.3 3292.4 557 4146 LPS029 4464.6 251.2 2334.42058.1 2930.3 3219.3 472 3814.4 LPS030 1142.2 352.5 2519.8 2460.9 2499.82634.5 378.3 2147.8 LPS031 1067.7 481.6 3510.8 2799.2 3568.2 3257.2287.9 2209.7 LPS032 1120.2 332.3 3153.1 3032.4 1769.2 1816.7 146.62689.9 LPS036 1498.2 1072.9 4633.6 5524.2 5465.1 6350.7 918 14058.5LPS037 1890.3 320.9 3719.1 3618.9 4138 4518.1 513.4 5087.5 LPS038 2899.5310.3 4530 4226.1 4491.6 3969 268.4 4245.3 LPS040 527.4 238.1 1433.41611.2 1984.5 1506.5 143.9 1988.7 LPS041 506.1 265.5 1958.9 2843.22065.3 2016.2 147.4 2781.7 LPS042 1432.1 1140.3 4379 4973.3 4525.44340.8 319.6 3009.6 LPS043 696.9 776.2 3933.1 4894.3 3512.2 3664.7 340.63098.4 LPS044 57.8 275.1 3365 4261.2 4773.5 4979.9 974.4 10645.5 LPS045536.1 211.1 1559.5 1415 1498.5 1584.8 562.1 1912.3 LPS046 796.3 231.71023.9 306.4 1417.8 1328.2 83.8 946.4 LPS047 5029.9 518.2 3632.5 4262.14755.5 4087.9 386.3 4933.8 LPS050 6333.5 2620.8 5271.4 5242.1 5586.45560.1 980.1 11444 LPS051 1378 224.4 2328.8 2221.8 2260.5 2715.1 123.73670.4 LPS052 1526.4 267.5 2046 1856.2 2186.5 2416.3 99.3 2010.1 LPS0534438.3 361.6 4087.6 3959.9 4786.5 3666.8 379.6 4256.7 LPS054 1992.9269.9 2734.2 2388.1 3143.8 2337.7 177.6 2803.9 LPS055 4587.8 334.43488.6 3474 4018.3 3101.6 196.2 4309.4 LPS056 5960.7 1333.7 5338.85670.3 5674.4 5533.5 446.4 5593 LPS057 2219.9 301.9 2397.3 2356.1 2218.12085.6 184.4 2657.8 LPS058 4070.4 299.9 3485.4 3721.3 4113.8 4142.2239.8 4945.6 LPS059 8729.3 279.2 3885.7 3636 2720.4 3346.7 165.7 3734LPS060 4580.2 323.7 3027.8 4713.4 4929.1 5047.5 161 4704.8 LPS061 2831.9366.8 2392 2327.7 2546.5 1991.8 177.9 3036.7 LPS062 1674.1 353 2711.22526.1 1847 1830.3 124.5 3584.2 LPS063 5514.4 419.8 5238.9 5020.3 5417.45041 250.1 4812.6 LPS064 7417 3166 5229.5 7497.4 7933.1 10261 1088.316829.6 LPS065 5634.9 343.5 5527.8 5099.4 7833.4 5356.6 237.5 4696.7LPS066 1015.9 244.5 1702.6 1650.5 2895.1 2437.2 128 2514.1 LPS067 2796.8240.4 3931.5 4810.3 5407.8 5418.3 202.5 9403.8 LPS069 533.4 189.9 1635.81816.4 2114.2 1646.8 119.8 3208.8 LPS070 2516.9 240.6 1909.5 2519.62156.7 1777.4 186.4 4362.1 LPS071 592.8 196.4 1789.2 2189.2 1981.11304.5 127.6 3430 LPS072 444.2 217.6 1422.9 1509 2065.3 2289.9 122.72678.8 LPS073 4362.8 273.1 3094.9 3348.1 3771.8 4075.3 137.7 4259.6LPS074 32072.9 6816.3 33531 25258.9 38176.4 32687.7 14607.1 37529.6LPS075 7013.9 472.7 4759.7 4933.9 5452.2 5408.7 409.4 5397.1 LPS0764236.1 362.6 3131.9 2882 3368.5 3354.6 119.5 3141.9 LPS077 2958.7 276.64380.4 4862.5 4475.1 4958.7 218.9 4426 LPS078 23685.3 2642.5 35458.625869.6 42378.9 33047.1 25402.2 37189.8 LPS079 4794.3 547.8 4628.64821.8 5257.2 5277 829.5 5449.7 LPS080 30454 10527 33713.7 23785.432590.9 32210.7 16224.4 37659.2 LPS081 30405.9 28677 35358.3 2587322338.1 31715.3 36436.4 36650.5 LPS083 5040.1 460.8 3251.7 3487.3 2688.92565.9 190.5 2979.7 LPS084 2031 298.9 2843.7 2718.4 2352.2 2165.5 164.93398 LPS086 3571.7 320.1 2715.8 2648 1989 2528.4 143.9 2969.7 LPS0873302.3 337.4 4873.1 5695.8 5407.2 5450.6 670.8 18404.9 LPS088 826.8302.1 2389.2 2871.1 3180.8 2635.2 138.6 3141.5 LPS089 796.4 321.2 1987.72640.6 3299.1 2285.1 143.7 3176.6 LPS090 4031 235.9 3867.3 4064.4 4503.34798.4 341.7 4697.7 LPS091 2423.3 196.5 2836.8 3101.3 4049.1 4172 295.24612.2 LPS092 2914.9 208.5 4005.3 3138.4 3911.6 4036.1 270.4 4842.9LPS093 793 195.5 1619.2 1331.6 1909.3 1843 147.1 2772 LPS094 1374 2212205.5 2028.5 2240.9 2632.2 163.3 2849.1 LPS095 728.7 174.1 2022.62112.1 2335.8 1264.6 117.5 2957 LPS096 333.3 168.5 1531.9 1393.4 1893.3869.1 118.3 1691.1 LPZ001 2008.6 185.4 2535.9 2937.9 3472 1981.8 118.92421.7 LPZ002 3529.3 384.6 4579.3 4474.6 3236.7 3855.8 313.8 3237.5LPZ003 4076.8 275.4 2651.2 2966.7 2829.2 4177.4 378.5 4369.7 LPZ004 5595687.4 5468.2 5615.9 5243.6 5699.6 601.6 5889.9 LPZ005 5680.5 335334994.7 26121.9 42555.1 33144.5 16193.7 37798.2 LPZ006 1199.8 299.43013.7 3099.8 3517.3 3397.1 140.6 3370.8 LPZ007 1159.1 462.2 3292.72992.5 3121.4 2936.7 235.5 3238.6 LPZ008 1874.3 237.7 3110.8 3236.72516.5 3182.2 325.3 4330.1 LPZ009 3331.1 296.3 2348.5 3414 2478.2 3309.5348 5658.1 LPZ010 3216.3 1186.8 4977.3 5024.7 4564.4 4992.4 442.6 4454.5LPZ011 4613.4 910.9 4510.7 4515.7 3729 4357.3 371.4 4695.9 LPZ012 1531.5469.5 2915.3 2611.1 2012.3 3481.4 270.3 3804.3 LPZ013 3495.1 268.82125.9 2584.7 3194.7 3787.4 125.1 4929.6 LPZ015 2040 257.6 1971.1 2966.72191.1 3056.7 227.1 4156.6 LPZ016 5307 2761.1 8451.7 17219.7 22792.715567.3 1073.6 35074.1 LPZ017 2476.4 354.3 3175.5 4330.8 4496.2 4061273.2 5328.9 LPZ018 3929.4 417.5 12420.2 14916.1 18116 17637.5 2541.631981 LPZ019 5404.2 427.3 32190.3 24710.4 42102.7 32342.6 19528 36969.5LPZ020 576.9 142.9 1451.4 1505.4 3534.8 2679.8 210.9 3046.2 LPZ0221408.2 155.2 2406.7 2845.7 3042.5 3074.8 189.9 3829.2 LPZ023 562.1 152.82096.7 1710 2045.5 2078.9 200.8 2874.3 LP2024 496.7 158.1 1681.3 1264.72102.9 1857.1 132.1 1818.4 LPZ025 5431.3 464.1 13492.2 9726.2 11911.513462.8 1262.5 11780.6 LPZ026 1663.2 139.7 2464.8 2760.1 3113 2219.4159.1 3183.5 LPZ028 5029 190.7 5367.2 5339.8 5483.9 5205.5 482.3 5565.9LPZ029 961.3 119.2 1805.4 1989.6 2298.5 1998.4 126 2576.9 LPZ030 1457.4177 2444.7 2687.5 1966.4 1857.2 178.5 3312.8 LPZ031 3092.8 361.7 35643925.3 4627.8 5171.4 506.7 5920.5 LPZ032 1906.5 156.8 5542.3 2434242917.8 33386.1 30058 37998.6 LPZ033 12934.5 354.7 5280.1 7301.2 5638.99238.7 375.4 15843.5 LPZ034 1307.4 177.5 1737 2208.4 3213.1 1984.1 150.23228.3 LPZ035 556.5 201.9 880.2 1280.1 1654.5 915.1 74.1 1422.1 LPZ0371356.8 269.7 2072 3110.5 2912.8 2488.2 211 4119.3 LPZ038 4027.9 426.95639.9 5872.3 5476.8 5614.6 796.8 5583.3 LPZ039 5059.1 550.6 3807.94393.8 3825.6 3889.8 342.2 5164.2 LPZ040 1226.1 236.5 1566.4 1889 1679.12263.6 140.6 3331.1 LPZ041 944.2 219.3 1629 543.1 1148.2 1416 90.22524.6 LPZ042 570.6 206.1 1129.5 806.5 1448.8 1423.1 75.1 2013.8 LPZ0431190.2 236.7 1878.8 1024.4 2834.6 2767.4 241.7 3236.2 LPZ045 5315.3465.7 4933.2 5580.2 5151.1 5205.1 557.3 10754.3 LPZ047 859.5 285.21606.2 2099.3 2059.4 1992.6 68.3 3054.8 LPZ049 3232.7 108 1278.6 2834.23657.8 3944 244.2 5459.6 LPZ051 3048.1 146.9 2373.2 2067.3 2745 2383.2179.1 2837.6 LPZ053 2580.3 135.6 2625.8 2088.7 2468.5 2297.2 156.83001.4 LPZ054 1838.1 159.5 2657.8 2759.7 2658.1 2224.7 170.4 3444.2LPZ055 2181.8 151.1 2381.2 2262.7 3228.3 2983.9 139.3 2673.9 LPZ0564028.3 219.5 2884.6 3416.6 3779.6 3789.9 208 4518 LPZ057 1470 121 1676.51629.6 1702.7 1703 112.2 2272.1 LPZ058 1923.3 122.5 2453.5 2169 3127.32465.4 160.6 3319.6 LPZ059 1760.4 113.8 2180.6 1832.4 1997.2 1530.8174.4 3366.6 LPZ060 3296.4 139.3 2571.1 2250.2 2721 2976.9 221.3 3896.5LPZ061 2495.6 182.8 2663.9 2235 3265.9 4227.1 498.1 4915.1 LPZ062 1992.7194.9 3296.7 3975.8 3861.5 5642.6 497.6 5606.2 LPZ063 2167.1 145.9 27331843.9 3066.6 4961 305.6 4773.2 LPZ065 5641.2 251.7 13690.3 9269.28562.8 13254 986.3 9554 LPZ066 6307.3 652.4 12630.8 6968.4 4918.9 5062.2400.7 5456.8 LPZ067 10838 1548.1 16986 11776.8 5633.2 7054 1014 15262.2LPZ069 1481.9 209.6 2239.8 1480.9 2496.7 2542.4 250.5 3717.2 LPZ0701932.5 263.8 1895.1 2221 1555.9 1570.4 145.5 3471.3 LPZ071 3672.6 378.64185.5 3050.5 4166.8 4246.2 553.7 5333.4 LPZ072 744.5 210 1210 676.71420.2 1393.4 95.8 1997.1 LPZ073 1997.9 235.9 2275.1 2141.7 2613.21989.9 170 3489.4 LPZ074 1375.9 237.4 1899.1 1787.3 2472.9 1623.7 125.62435 LPZ075 831.4 247.9 1536.4 1773.1 1886.9 920 80.6 1053.5 LPZ076345.7 251.8 854.8 564.6 1747.1 526.2 55.9 1058.3 LPZ077 2466.3 102.2949.4 820.9 3093.9 3179.6 202.9 3314.8 LPZ078 3102.1 197.1 3654.2 32614204.3 4433.6 400.8 5559 LPZ079 1584.4 108.3 2389.2 2243.3 2624.8 2677.1208.3 3675.6 LPZ080 12206.5 2043.1 25021.4 8579.5 11707.8 8717.6 117218663.9 LPZ081 1368.7 103.6 1902.8 1349.9 2166.1 1597.7 103.5 2709.6LPZ082 2601.3 140.3 3264.3 2853.9 2799.6 1742.3 251.1 4288.2 LPZ0831311.9 76.7 1622.4 1071.1 1733.9 1878 104 2007.7 LPZ084 9974.7 801.314255.3 8399.1 5763.9 8852.9 542.2 5714.3 LPZ085 4609.8 158.4 3923.33729.7 4082.8 3867.3 219.3 4075.1 LPZ086 10874.1 987.4 19189.5 8284.65646 9109.8 1116.4 14988 LPZ089 3505.8 211.6 4010 3430.6 3762.1 3770.8224.3 5341.2 LPZ090 5780.9 581.8 13217.4 6303.4 4694.8 4779.9 425.25408.9 LPZ091 5316.1 148.4 2263.4 2139.8 2382.2 4067.2 256.8 14732.6LPZ092 5448.7 209.4 3631.6 4152.7 2934.1 3403.7 174.9 4943.6 LPZ093 1169159.4 2097.9 1187.4 2050.8 2350.7 109.4 2605 LPZ094 1245.5 139.7 1547.51650.5 1875.2 2009.9 80.2 2376.9 LPZ095 711.2 177.9 900.9 1253.3 1013.81395.3 48 1586.1 LPZ096 2122.2 249.7 2929.3 3271.3 2132.9 2224 232.84443.8 LPZ099 4306.4 211.2 2603.1 2144.4 3479.2 3488.5 138.1 4085 LPZ1003373.5 297 3941.3 3149.6 3790.4 3857.5 443.8 5028.1 LPZ101 3007.7 272.43546.9 2291.3 4299 3232.1 306.1 4819.6 LPZ102 2092.7 324.7 3167.5 2109.33524.3 2829.4 279 4297.4 LPZ103 3602.1 285.7 2923.3 3112.9 2812.9 1318.387.9 1739 LPZ106 1359.7 305.1 2680.3 2391.6 2838.5 2097 173.7 3009.6LPZ107 28560.8 4989.5 20821.7 17880.4 39173.1 27035.1 11973.3 36123.4LPZ108 4136.8 179.4 4259.8 4978.2 5553.2 4862 837.2 5597.5 LPZ109 3708.3202.4 3842 3510.4 4638.4 4453.7 469.5 5107.4 LPZ110 4557.2 291.4 5020.64801 4487.4 4481.1 552.3 5484.2 LPZ111 1625.6 130.9 2242.1 1982.7 2740.62455.4 164.6 3722.3 LPZ112 2887.4 195.8 3813.2 3759.4 3984.8 4167.1409.7 5461.8 LPZ114 5029.5 213.4 5016.7 4678.8 5036.9 5168.1 302.1 4316LPZ115 24434.4 2637.1 27958 23684.2 41104.3 30920.9 2153.9 36902.6LPZ116 8682.9 235.7 5647.3 5316.6 5805.6 9313.7 466.6 16018.9 LPZ11730879 4843.7 36277.1 24358 24673.1 20545.7 4669.9 5652.6 LPZ118 4023.6171.1 3743.5 4568.2 3845.4 3783.9 254.3 4782.5 LPZ119 2580.4 114.12507.2 3114.1 2544.6 1963.8 127.6 3195.4 LPZ120 1998.8 157 1987.2 1503.12331.8 1805.1 131.5 3522.3 LPZ122 2041.4 119.6 2145.6 2430.9 1998.62171.8 101.3 2677 LPZ124 2795.6 185.4 2980.4 2672.5 2495.2 3459.4 173.13081.5 LPZ126 2559.7 181.8 2560.1 2349.8 3500.6 2362.1 224.9 3646.9LPZ127 1993.5 169.1 3161 3180.8 3382.5 3321.3 180.6 4058.4 LPZ128 2866.7263.2 3556.8 3597.4 3545.7 3813.8 306.7 4071.3 LPZ131 1993.5 171.71983.9 2069.6 2565 2607.2 80.3 2527.8 LPZ133 2446.7 290.4 3218.6 2847.23830.1 2889.5 245 4252.4 LPZ136 1952.3 281.1 2956.9 1870.6 3167.6 2680.6215.9 4291.6 LPZ137 2833.8 281.9 3264.4 2350.2 3874.4 3532.8 420.84935.3 LPZ138 2932.9 1791 5211.5 4502.1 5409.9 4832.8 543.1 4741.3LPZ140 2284.7 337.4 3680.2 2810.9 3196.1 3191.2 271 4613.6 LPZ141 4726.2368.5 4792.5 4412.5 5368.1 5466.3 722.1 4956.4 LPZ143 25290.6 2692.235967.9 25679.9 43668.3 32612.1 25456.9 36344.4 LPZ144 2620.9 286.63948.7 3394.6 4505.7 4142.8 488.7 4776.7 LPZ145 3472.5 171 3949 3194.23430.5 3539.9 327.9 4487.2 LPZ146 2612.8 127.3 2482.4 2080 3000.8 2979.1135.1 3391.3 LPZ147 2447 106.3 2855.1 2237.7 3134.2 2841.8 261.6 4388.1LPZ148 2036.8 77.7 2559 1932.3 4296.1 4699 359.6 3982.3 LPZ149 5720.7267.4 5377.3 5408.2 10999.7 5717.7 1078.9 13033.2 LPZ150 5861.7 77235541.7 26314.8 44633 33238 13126 37853.6 LPZ151 5550.3 3499.3 9012.88380.4 11968.1 5716.5 715.8 5536.9 LPZ152 4746.6 352.8 5169.3 5647.75384 5394.2 408.5 5382.1 LPZ153 21881.2 2773.2 14738.2 15979.5 16996.815756.8 2388.5 30812.9 LPZ154 4869.8 265.9 3244.3 3497 3948.6 3703.3303.8 4119.1 LPZ155 3904.2 1596.3 5078.5 5482 4631.7 5314.1 553.4 4112.9LPZ157 4726.5 1732.8 5427.1 5369.5 5213.3 5705.9 756.4 5462.2 LPZ15815297.4 3817.1 17993.9 17405.3 25168.8 22056.6 2337.4 22375.3 LPZ1625725.8 4204.8 10380.1 11364 17948.2 14250.8 1934.6 10535.5 LPZ165 5615.2666.7 5274.6 5486.6 5560.3 5310.9 637.1 5405.9 LPZ166 5889.1 2603 9503.510943.7 13743.3 14080.4 1772.5 5772.8 LPZ167 5347.2 1948 5708.9 6769.65742.3 5347.9 370 5279.7 LPZ169 3043.8 267.4 1976.6 2851 3451 2451.2189.8 3420 LPZ170 3507.3 301.5 3532.3 3391.4 4481.4 3398.7 130.2 5604.2LPZ171 3762.3 780.7 4554.8 4311.7 4936.4 4511.3 398.6 5030.5 LPZ1725098.2 947.4 5550 6287.9 5135.4 5323.9 1242.5 8539 LPZ173 22580.5 3313.935486.9 24974.9 42874 31828.8 26531.8 36066.9 LPZ174 4115.7 221 5241.54262.4 5765.8 5554.9 872.5 4815.2 LPZ175 4388.3 1360.6 5563.7 5504.95165.3 5182.9 583.2 4602.3 LPZ177 1371.6 94.5 2119.4 2218.6 2730.72431.7 143.2 2893.2 LPZ179 3643 195.1 4409.9 4898 5458.3 5319.8 797.95677.7 LPZ181 5573.3 215.9 4799.6 5272.2 5825.3 5554.4 1573.5 13689.7LPZ182 4118.9 107.6 3491.5 3182.1 4617.5 4543.6 478.1 5527.8 LPZ1865792.2 325.5 4965.1 5182.6 12373.6 11191.4 1804.9 37336.4 LPZ189 33820.35188.5 30941.4 24955 43453.2 33115.2 17929.3 38055.6 LPZ194 2807 151.12915.9 2955.1 3306.8 3120.2 142.9 4101.6 LPZ195 5345.7 532.7 5597 5628.75540.4 5491 545.7 5756.7 LPZ196 4805.1 3512.9 5183.2 6968.6 5465.45052.4 786.4 5694.5 LPZ197 7268.6 153.8 5398.9 5673.5 13582.6 15111.93499.6 34684.8 LPZ198 7208.3 210.6 5800.8 8043 5439.2 5183.6 409.35042.7 LPZ199 3058.3 186.1 2749.6 2667 3713.6 3704.3 243.4 3917 LPZ2017175.3 236.7 4827.6 5029.9 5523.4 5802.2 1981.9 14614.5 LPZ202 3603.31113.9 35531.5 26035.1 44762.8 33837.3 63521.8 38225.4 LPZ203 4325.4424.4 5517 5387.3 9934.8 5662.1 2104.8 9370.7 LPZ204 32355.9 3469036443.6 26004.3 44546.1 33680.5 55702.6 37890.3 LPZ205 4904.1 519.65162.4 5398.7 5427.6 5325.6 281.4 5770.2 LPZ206 3504.4 319.8 3124.84561.7 4192.2 3899.9 255.7 5489.9 LPZ207 32035 24978.7 34825 23371.842639.9 32686.4 30672.2 37674.8 LPZ208 25174.6 3118.6 14244.4 13906.316694.7 21111.9 2190.1 34542.4 LPZ210 3885.1 422.3 3895.8 4551.5 4205.75108.7 258.6 5514.3 LPZ211 2569 176.7 3689.2 2943.5 4001.9 3860.9 250.23113.1 LPZ212 5988.8 1244.3 32684.4 11154.1 19853.4 13654 618.3 10736LPZ213 3406.9 106.8 3964.1 3876.6 4236.4 4294.4 274.2 4874.6 LPZ2141668.3 55.3 2136.3 2394.8 2390 2269.3 105.8 3436.2 LPZ215 5019.8 139.35020.1 5024.8 11013.9 13747.1 1991.7 36930.6 LPZ216 3336.8 1085.435895.6 26245.3 44980.1 33834.4 64482.7 38238.1 LPZ217 23512.1 26363.336065.8 24685.4 43193.4 31422.4 21462.1 35990.6 LPZ219 4011 256.9 3193.53326.3 4509.5 5258.5 455.2 5841.9 LPZ220 8696.5 2383.3 5064.7 5171.34923.7 5340 951.7 17530.6 LPZ221 1221.4 83.1 1201.8 707.6 1556.5 2083.9182.1 3948.8 LPZ222 1885 146.1 2834.7 2253.2 2557.7 3382 196.7 4225.1LP2223 1048.5 121.2 2339.6 2642.1 2663.8 3573.5 383.5 4579.2 LPZ2243190.6 118.8 3049.8 2833.2 4373.8 5139.9 858.6 5285.7 LPZ225 25428.24079.7 35724.5 25423.6 43300.5 32574.8 38888.3 37219.9 LPZ226 1044.9130.4 1776.9 1210.8 2757.7 3388.5 326.6 3520.3 LPZ227 1078.3 133 1461.7973.5 7032.1 9452.8 2043.5 4705.3 LPZ228 3961.6 213.5 3373 4050.7 5575.610714.4 2428.6 5928.9 LPZ231 3475.2 230 4096.3 3841.1 5009.3 5690.3959.9 5514.3 LPZ233 2404 218.2 2170.9 1531.4 4362.7 4198.1 673.7 3350.1LPZ234 1688.3 312.3 1887.6 1486.5 4228.6 4715.6 724.8 3170.1 LPZ2352661.6 199.9 2422.2 1852.6 3078.9 2886.6 98.2 3143.5 LPZ237 3174.5 324.23032.5 2988.2 3931.1 4587.9 314.4 4588.3 LPZ239 4061.3 309.3 3175.12932.1 4131.7 3892.6 122.3 5083.5 LPZ240 3799 316 3730.6 3314.6 3379.83538.8 212.4 4784.5 LPZ241 2559.2 62.1 2610.4 1794.5 4165.6 3754.4 134.82915.1 LPZ242 29360.5 3262.9 35254.6 25196.9 43028.8 31468.2 7308.436768.4 LPZ243 3405.3 88.8 3015.7 2683.4 3678.7 2990.6 121 4001.4 LPZ2444856.9 483.6 4842.2 5235.3 5317.6 5432.1 205.4 5712.9 LPZ246 1274.8 65.92301.7 1922.8 4332.2 4628.8 672.1 4232.4 LPZ247 3894 69.8 2522.8 3389.94451.4 4937.1 939.3 5522.8 LPZ248 3016.7 268.6 2883.2 3805.2 3791.73777.6 487.1 4585.6 LPZ249 5224.1 138.3 3524.5 4091.2 3022.4 3393.2149.9 4101 LPZ250 1060.6 46.5 1400.9 1246.9 1419.5 1411.2 118.8 2908.4LPZ251 1336.8 248.5 1354.6 1049.3 657.6 924.3 70.3 2064 LPZ255 3787.8171.8 4801.8 5076.3 4608.1 4965 340 5636.6 LPZ256 536.6 61.6 865.5 971.61130.8 1327.7 82.7 936.9 LPZ257 844.5 112.6 1507.4 1537.8 2337.9 2745.8341.5 1610.7 LPZ258 2588.5 142.3 3443.1 2902.2 4576 4976.4 1182.9 3619.6LPZ260 837.7 113.7 1677.9 944.3 1217.6 1286.6 170 2928.2 LPZ261 981.3132.1 1499.6 743.4 1590.8 1953 67.2 1652.1 LPZ264 4559.3 231.3 4348.52856.3 4869.2 5179.7 412.3 4698.3 LPZ265 21063 2793.9 26928.5 12365.513816.5 12134.4 691.6 17954.8 LPZ266 1642.4 130 1767.5 1463 1633.81410.5 59.4 1444.5 LPZ268 2451 114.2 2803.3 2495.4 3126.5 3433.7 794261.3 LPZ269 15670.7 3660.5 35782.4 21720 40375.2 31597.3 2024 35213.6LPZ270 3541.2 240.8 3803 3132.4 4827.8 5213.6 79.9 5473 LPZ271 5590.7677.2 5465.4 5197.1 5703.4 5615.2 309.2 5732.7 LPZ272 27369.6 3445.935824.6 22832.6 40684.8 27398.4 1732.9 37016 LPZ273 1107.3 46.1 456.7336.3 1879.3 1654.1 65.4 971.9 LPZ274 3936.2 114.5 3192.3 3024.1 4983.34907 293.9 4933.5 LPZ275 2567.2 42.9 1760.4 2091.8 3656.5 3800.5 772585.4 LPZ276 560.9 32.9 1075.4 1878.9 1889.9 1766.2 66.8 1294 LPZ277423.7 34.6 1199.1 1169.8 1376.8 1383.9 91.9 1123.7 LPZ278 323 39.7 937382.9 770.1 935.2 66 1403.4 LPZ279 965.9 70.7 1907.5 1368.2 1783.71603.9 133.2 2438.8 LPZ280 390.7 19.6 175.4 42.3 464.9 631.5 29.9 2074.9LPZ281 84.3 8.2 0 0 0 0 9.7 0 LPZ282 1849.7 28.4 315.1 34.2 664.3 1097.321.5 1229.5 LPZ283 10678.6 329.2 5134.7 5311.1 4772.3 8591.1 226 9633.6LPZ284 996.1 39.8 236 147.2 2349.5 981.1 26 719.7 LPZ286 563.8 77.11031.1 945.9 1347.4 1601 81.2 1303.6 LPZ287 1045.7 123 2057 1475 1730.93003.6 149.9 2493.5 LPZ288 1201.7 116.2 1797 1448.8 1648.3 670.4 80.63700.4 LPZ289 1922.3 113.3 2515.4 3395.3 3460.7 3369.4 70.8 2183.8LP2290 14629.5 3945.8 34659 24047.3 40474.8 27786.2 1348.2 27566.4LPZ293 4364.8 385.4 4664.2 3170.9 4321.6 4789.8 74.6 5095.8 LPZ294 564.7171.4 1257.5 705.2 1357.7 1610.2 18.6 2027.6 LPZ295 823.1 97.3 2102.71056.2 2899.7 2698.3 39.4 2448.2 LPZ297 5273.4 169.1 5229 5074.4 5727.811512.9 423.2 10966.8 LPZ299 1564 161.1 1743.9 1752.3 2764.1 2660.5 63.52791.9 LPZ300 3068.3 205.4 2406.8 1881.8 2898.6 2758.4 0.2 2007.2 LPZ3011979.7 233.1 3207.1 2109.3 4343.5 3713.8 40.4 2690.6 LPZ303 509 32.7281.3 877.7 893.1 751.5 30.1 1373.8 LPZ304 2531.1 289.3 3809.4 3406.73674.8 3517.4 158 2652.7 LPZ306 22632.7 2861.2 34933.8 25435.6 40453.930906.9 1505.9 34032.8 LPZ307 2604.4 1395.2 4780.6 6945.3 4419.2 4416.9232.6 4299.8 LPZ308 1093.9 60.5 2028.1 1751.6 1770.8 1891.9 92.4 3245.8LPZ309 286.1 26 480.4 378.4 589.6 731.4 38.4 1062.4 LPZ310 2284.1 129.51622.7 1091.7 1207.1 3089.4 101.2 3624.9 LPZ311 3309.9 43.6 2782.62956.3 2828.8 4446.7 95.8 5593.4 LPZ312 446.3 52.7 1577.7 1221.4 542.2518 56.1 1952.6 LPZ314 378.6 26.9 333.9 355.8 682.2 701.2 61.7 732LPZ315 3897.5 115.2 2611.9 3145.8 4296 5240.2 151.3 4499.3 LPZ318 9709.6767.1 19964.9 15678.3 20611.8 19600.2 475 18079.9 LPZ320 1126.7 82.81215 1002.7 1502.5 1555.3 67.5 2964.4 LPZ321 2944.7 85.4 2590.7 2597.62550.3 2962.7 72.1 5481.4 Clone ZE1 ZE2 ZE3 ZE4 ZE5 ZE6 ZE7 ZE8 ZE9.1LPS001 369.9 369.9 369.9 369.9 369.9 369.9 369.9 369.9 369.9 LPS003600.3 363.9 0 243.7 1565.3 2624.5 1942.7 242 1892.5 LPS004 522.3 254 074.6 907 2638.8 1933.6 274.9 4209.2 LPS006 444.6 161.2 0 174.6 793.62651.4 1991.5 206.5 598.8 LPS007 528.9 136.3 0 244.9 1623.3 1202.12044.7 245.1 213.9 LPS008 534.5 215 0 281 1231.2 783.4 1760.3 178.5832.4 LPS010 469 183.6 1.3 240.1 947.7 591.6 2208.3 161.2 482.6 LPS011468.7 93.3 0 142.3 1544 1021.5 2334 254.6 1223.7 LPS012 511 278 17.7197.2 2129.6 68.9 1362.7 478.9 960.4 LPS013 478.2 407.1 192.1 235.62470.6 9 1163.9 885.7 1109.4 LPS014 579.7 369.1 0 272.7 2799.6 1525.72222.4 606.9 2638.6 LPS015 419.7 254 0 2380.7 7188.1 4998.4 16519.65245.1 15550.4 LPS019 1068.4 279.6 0 396.2 3848.5 3074 3866.9 959.13664.7 LPS020 314.2 109.1 0 102.7 2036 234.4 1504.2 319.6 1053.2 LPS023364.9 104.2 0 100.7 1151.8 0 1253 175.5 570.5 LPS024 804.7 213.8 0 346.33248.5 2523 2722.6 915 1987.4 LPS025 1374.7 407.8 0 857.1 4731.2 2584.24119 1138.4 2458.4 LPS026 337.6 86.1 0 100.2 1242 0 1052.9 242.9 988.9LPS027 440.5 182.5 0 118.5 1318 691.2 1274.1 226.1 385 LPS028 369.5166.2 0 168.7 2587.7 2597.8 4035.5 565.9 1883.1 LPS029 323.4 141.9 0165.3 2524.3 2147.2 3031.3 567 2263.9 LPS030 362.3 226.5 0 169.6 1528.2422.9 1236.7 239.2 1049.1 LPS031 591 536.7 4.9 383.6 1768.3 850.4 1013.3399.8 781.4 LPS032 443.9 327.3 0 328.1 3200.9 1880.1 1832.8 265.6 1391.6LPS036 1093 781.8 24.5 680.8 3911.3 3750.9 3746.7 661.8 3856.4 LPS037501.6 180.4 0 200.9 2664 2369.8 1960.5 339.9 2892.9 LPS038 1180.1 471155.9 1679.7 4392.3 2103.5 3019.9 800.3 2819 LPS040 398.8 108.6 0 103.91030.7 195.9 1566.1 144.4 682.1 LPS041 384 153.8 0 149.7 989.2 1257.52235.4 143.3 1228.4 LPS042 1381.9 951.7 44.9 716.1 3682.1 2755.7 4011.7508.5 2963.4 LPS043 1211.6 704.8 74 613.6 3494.4 2435.3 3362.1 391.41544.7 LPS044 361.3 100.2 0 142.1 2244.4 3031.8 2653.5 393.4 1620.1LPS045 285.7 75.2 0 64.4 856.5 223.5 1616.1 216.8 609.7 LPS046 325.8217.2 0 70.2 1758.6 0 1280.9 284.9 1115.5 LPS047 2041.3 1347.8 768.41080.5 4169.9 3927.9 4263.5 1831.6 4804.9 LPS050 3226.4 3356.4 6064.53347.5 9841.4 3046 5362.2 2924.8 5821.2 LPS051 377.1 96.4 0 156.8 2452.62286.5 3035 396 2238.1 LPS052 330.1 80.1 0 162.6 2418.1 0 2097 352.51677.3 LPS053 402.6 160.1 0 146.3 2249.5 56.9 1986.1 464.3 1349.6 LPS054497.4 147.5 0 184 2188.4 379.1 1976.1 308.6 1558.9 LPS055 1168.2 645.7 0354.9 3901.1 1476.5 2607.5 774.8 3026.1 LPS056 1549.5 1243.3 37.9 752.64770.9 3403.7 4086.8 1204.2 4958.4 LPS057 387.5 154.3 0 262.7 2612.2502.6 2317.5 365.1 1418.8 LPS058 671.2 198.9 0 434.8 4189.9 2258.63366.3 586.3 2190.5 LPS059 726.2 207.5 0 304.6 2974.8 2054.3 2712.8395.1 1331.6 LPS060 534.2 215.8 0 221.7 2896.9 718.3 2693.3 477 2474.9LPS061 530.8 369.4 0 204.3 1801.1 1286.4 1533.6 298.7 1327.2 LPS062407.4 305.2 0 226.4 1509 0 1413.1 212.5 954.6 LPS063 619.4 280.8 0 2823987.4 1805 2589.9 642.1 1650.4 LPS064 3689.2 4982.4 10201 3080.3 8359.83622.3 8304.6 2997 13781.1 LPS065 466.4 189.7 117.3 817.1 4336.3 2332.64393.4 1092 3866.8 LPS066 269.5 104.6 0 131.4 1006.2 76.7 1834.6 185.9668.5 LPS067 426.4 179.7 49.3 341.3 4153 4077.7 5101.3 1195.9 3894.1LPS069 367.8 136.7 0 128 1456.6 0 2685.6 308.7 1234.3 LPS070 438.5 137.30.4 111.6 1932.5 25 3005.3 210.4 721.5 LPS071 283.9 83.2 8.5 109.21831.5 0 3634.2 302.8 708.3 LPS072 301 147.2 5.7 132.9 1600.8 592.33051.5 331.5 1173.5 LPS073 692.1 485 251.5 497.9 4205.3 2827.4 3777.9740.1 3882 LPS074 36280.3 66359.2 63362.2 44047.1 47176.9 20938.964534.5 33666.4 78457.8 LPS075 3204.8 1250.6 650.9 1033.7 4976.3 4377.14632.6 1617.1 5570.9 LPS076 434.7 127.9 0 204.7 1731.3 419.1 2737.8298.8 2175.1 LPS077 416.6 107.6 0 327.2 3360.7 1950.5 4020.1 609.13713.9 LPS078 5164.5 1194.3 906.3 6556.7 20779.8 7364.3 28847 868022339.1 LPS079 1317 501.9 304.2 893.4 5047.6 3196.6 4887.5 1058.8 4992.1LPS080 27721.4 56038.2 70896.6 26826.4 43426.4 20557.3 47819.2 16935.568145.5 LPS081 36397.3 66337.3 48195.9 41685.3 46187.5 20628 66138.131620 78253.7 LPS083 844.6 534.2 123.6 305.3 3724.2 1699.7 2524.6 583.83266.4 LPS084 665 249.4 0 334.5 2570.6 1491.7 2893.2 342.8 2061.7 LPS086456.6 155.8 0 165.1 1962.7 754.5 1931 130.2 2176.5 LPS087 967.5 450.717.2 633 4238 3720.3 5373.9 1754.7 19094.1 LPS088 468.4 276.1 0 151.11109.4 0 1779.7 302.8 2497.2 LPS089 329.2 316.9 0 133.6 988.7 0 1619.6320.7 1616.9 LPS090 478.9 272.3 0 218 4486.4 2182.3 2923.1 584.6 2640.7LPS091 385.7 177.9 0 290.7 2923.3 2008.6 2453.2 441.5 2246.6 LPS092396.3 164 0 345.2 2249.1 1219.1 2906.7 413.9 1589.3 LPS093 308.3 164.524 98.7 262.3 427.8 2140 175.4 661.4 LPS094 331.6 179 54.2 146.4 773.3948.2 1729.1 116 1030.7 LPS095 363.7 157.7 46.3 142.5 967.7 341.4 2639.7199.3 1055.4 LPS096 266.9 90.6 0 59.3 676.5 0 2616.2 136.5 215.6 LPZ001270.9 49.7 21.1 121.2 1958.2 496.8 4495.7 325.6 557.7 LPZ002 491.7 231.8157.9 345.8 1929.4 1183.4 3243.9 305.5 932.6 LPZ003 632.6 407.2 342.3343.5 2630.6 2108.3 3212.8 423 4663 LPZ004 2034.4 2260.7 1487.4 1442.55730.7 2135.4 5424.1 1804.4 5786.1 LPZ005 6301.3 4683.6 2801 10127.531972.9 7747.5 51335.5 17767 52067.8 LPZ006 471.7 131.2 0 179.4 1964.11552.2 2977.9 333.5 2954.9 LPZ007 584.6 383.7 69.3 294.3 1424.1 5312664.9 265 3021.7 LPZ008 325.4 87.5 14.4 176.8 1966.6 1588.5 2420.1199.5 3481.2 LPZ009 451.3 281.6 143.4 362.3 3149.2 4280.4 3006.5 357.54395.9 LPZ010 1324.5 1442.8 621.8 931.8 4310.4 3238.7 3926.9 617.44912.5 LPZ011 1740.9 2073.5 1436.2 1075.8 4631.4 5232.7 4563.3 1080.65456.6 LPZ012 424.4 217.8 50.1 271.8 2286.7 713.5 1791.4 390.6 3209.9LPZ013 395.4 123.8 33.1 181.1 3456.8 2121 2898.4 428.9 2673.3 LPZ015490.7 210.7 60.8 130.8 2889.2 330.5 2123.4 230.2 2680.6 LPZ016 2411.9710.9 346.5 1201.2 6897.9 4057.6 13340.1 3246.1 16664.2 LPZ017 635 257.436.2 247.2 2797.3 1219.1 3508 558.7 3953.8 LPZ018 1405.4 474.9 214.93188.7 8143.7 4992.8 12908.9 4208.2 14318.9 LPZ019 3487 975.9 698.48916.5 23680.5 15131 31656.3 14191 45343.3 LPZ020 251.9 195.6 0 40.91448.5 860.4 1113 305.5 971.2 LPZ022 250.1 112.4 0 133 2085.9 1282.52538.5 532 613.2 LPZ023 355.5 122.6 22.9 47.8 224 879.4 1419.1 132.3605.4 LPZ024 366.5 108.9 45.7 94.8 225.3 723.1 1276.8 81 319.5 LPZ025705.4 278.6 202.1 716.7 5145.5 4563.4 7215.5 1581.9 4195.8 LPZ026 268100.6 9 92.1 1164.5 750.4 3973.6 275.5 642.3 LPZ028 386 174.2 96.8 374.94188.4 2733.8 7024.6 1186.8 2375.6 LPZ029 221.9 86.8 0 47.8 225.3 264.42172 162.4 957 LPZ030 319 166 67.6 146.9 801.6 1385.4 2283.4 145.81189.4 LPZ031 2010.6 881.7 538.1 754.3 4625.8 3395 4051.7 1327.9 5202.1LPZ032 36097.5 35972.1 13659.1 19975.4 45544.5 20035.8 63759.8 31117.478268 LPZ033 1813.8 433.6 243.7 1171 7402.4 2278.7 10670.5 2204 5643.8LPZ034 332.6 97 34.6 181 1097.2 0 2704.1 262.6 3481.4 LPZ035 248 60 071.4 1188.8 0 1332.5 153.6 3028.4 LPZ037 375.8 133.8 0 114.6 3024.3909.1 2350.6 248.2 3546.8 LPZ038 577.7 237 44.3 370.7 4484.6 3572.5 4266907.9 4571.4 LPZ039 965.6 406.7 361.2 537.9 4020 2304.1 4269.9 8163717.5 LPZ040 399.9 127.4 0 88.3 1200.5 365 2123.2 244.3 1955.4 LPZ041318.4 105.1 0 136.6 856.3 716.6 1528.5 245.6 1538.5 LPZ042 289.3 77.60.9 189.5 441.5 365.4 1007.7 239.2 1212 LPZ043 417.3 166.7 57.4 158.21197.2 1617.9 793.5 569.1 2018.7 LPZ045 754.8 310.3 152 691.5 4810.43305.3 4043.7 1476.3 3925.7 LPZ047 270.5 155.4 53.1 39.1 2165.6 579.6980.9 361.7 1036.3 LPZ049 809.6 381.9 0 461.4 4406.4 2277.4 4764.62257.8 5528 LPZ051 333.1 121.5 0 56.6 1597.8 1677.8 891 270.7 1134.6LPZ053 271 119.7 0 16 1662.4 2447.8 1202.4 201.1 827.1 LPZ054 345.4 13161.7 79 1181.1 2238.5 1426.5 156.5 627.5 LPZ055 291 78.1 102.9 63.5551.3 2343.8 1433.5 193.6 814.2 LPZ056 364.6 167.9 83.6 130.3 1816.92580.4 2589.4 343 1579.8 LPZ057 250 76 0 11.9 426 457.6 2589.6 113.4709.8 LPZ058 231.1 40.8 6.2 44.4 454.7 163.4 3403.4 208.5 1300.4 LPZ059239.2 78.6 0 15.7 189.7 267 3272.7 141 1439.2 LPZ060 235.1 26.7 29.435.6 524.8 1238.2 2231.2 182.5 1908.8 LPZ061 402.1 268.4 141.6 254.61694.1 3088 2343.5 557.3 4157.7 LPZ062 727.7 146.8 0 203.3 2873.3 2418.93109.6 926.6 5812 LPZ063 316.7 108.2 25 190 1837.3 1025.1 2727.2 421.84195.9 LPZ065 512.5 59.9 94.9 583.5 5694.4 3741.6 5366 1221.6 3911LPZ066 622.2 66.8 23.8 405.6 6932.1 3089.5 4804.9 642.2 4079.9 LPZ067883.7 218.7 0 358.7 4518.2 2342.9 5200.8 1318.4 5539.6 LPZ069 335.6100.2 0 46.8 0 0.7 1736.5 255.4 2910.4 LPZ070 422.6 143.3 49.6 168.81231.5 1170.6 2432.2 262.7 2122.5 LPZ071 356 71.9 0 299.7 2303.6 1909.82575.9 447 2827.6 LPZ072 206.5 32.6 0 71.7 0 0 1362 189.6 907 LPZ073374.2 129.6 32.1 125.8 307.4 691.8 1617.6 282.4 1306.9 LPZ074 434.7 86.50 76.6 1671.1 0 1112.5 232.7 663.5 LPZ075 298.5 173.5 0 53.5 4083.2 01143.9 113.7 335.1 LPZ076 209.9 83.1 7.2 7.4 2185.9 0 490.5 226.6 362.9LPZ077 813.4 558.3 0 339.1 3733.4 4279.8 1115.9 671.7 4136.8 LPZ078 532349.8 0 265.8 4460.1 3290.7 2776.8 686.3 3283.3 LPZ079 347.8 183.6 11.653.3 2115.4 1191.7 1451.2 160.5 1233.2 LPZ080 948.1 264.9 178 455.14633.9 2869.1 4230.5 1378.5 5566.5 LPZ081 313.7 96.1 100.2 59.7 1161.61470.2 1119.7 98.5 710.9 LPZ082 260.9 120.3 18.4 68.3 1734.5 1430.61581.6 160.1 379.4 LPZ083 284.9 134.6 12.6 7.8 1067 906.1 1482.7 107.2790.5 LPZ084 1493.3 437.1 0 788 4497.3 4608.3 5078.9 2267.8 5087.7LPZ085 468.1 139.4 0 62.1 1618.8 1355.9 4314.9 340.5 2421.5 LPZ086 601.391.6 11.9 332.9 3540.3 3354.6 6675.2 781.2 5545 LPZ089 457.1 124.1 26.2267.7 2378.1 3364.6 3390.2 448.7 2930.7 LPZ090 436.6 50.7 0 149.1 3135.32087.8 3090.4 483.8 3408.1 LPZ091 350.2 91 96.2 99.2 1524.3 1976.3 3215315.9 4532.8 LPZ092 387.6 33.6 0 114.4 2386.7 2600.9 2845.7 274.6 2036.7LPZ093 195.6 25.8 0 27.1 2343.1 321.7 2875.2 252.7 1428.2 LPZ094 274.842.8 0 35.2 886.9 0 2502 152.6 1571 LPZ095 288.6 91.5 0 14.1 3.5 02317.2 144.4 1427.4 LPZ096 475.4 138.7 13.1 199.2 1690.8 1415.2 3939.2207.2 1889.7 LPZ099 428.1 73.8 0 185 2531.8 2596.6 3925.9 399.8 1967.5LPZ100 474.3 131.1 24.6 266.6 2710.6 2276.7 3358.6 503.5 2245.1 LPZ101492.1 101 81.9 233 2341.4 2076.1 2199.9 423.1 2022.4 LPZ102 477.3 133.20 167 3722.9 1604.4 2432.1 386.5 1199.8 LPZ103 353.5 90.7 0 108.9 4756.8214.4 1447.5 203.9 760.7 LPZ106 534 199.4 58.6 117.6 3052.4 1259.2 1610277.6 1224.3 LPZ107 29718.3 56194.4 31132.5 35651.5 44972.5 20589.638396.3 32828.7 75965.1 LPZ108 852.4 433.1 161.4 417.4 4354.8 2259.13536.9 1168.5 3161.8 LPZ109 554.2 248.6 83.8 254.6 3303.4 2521.1 1972.8608.5 2858.5 LPZ110 614 203.3 166.1 182.1 3236.7 2792.6 2764.1 455.53139.2 LPZ111 349.5 167.7 77.4 82.9 1407 1386.1 1469 200.9 1397.5 LPZ112497.3 279.2 65.3 242.6 3553.5 2504.1 2454.6 328.3 2182.2 LPZ114 890.2346.7 0 399.3 4520 3367.9 2902.7 1229.6 3474.1 LPZ115 24782.8 12016.11401.9 12188 32718.2 17087 38203.9 17191.1 25318.5 LPZ116 1388.1 392.7 0884.6 8895.4 3131.2 15554.1 2195.7 5401.6 LPZ117 6228.4 4810.2 389.71298.8 4199 2671 5473.5 1488.2 3911 LPZ118 424.5 267.3 39.9 196.2 2507.42210.1 2856.3 370.3 2640.4 LPZ119 295.1 183.4 0 59.8 2443.3 1153.2 2040158.7 1132.1 LPZ120 213.5 88.4 49.2 185.9 1336.6 1390.4 1604.5 186.71360.8 LPZ122 317.6 120.3 0 92.1 1390.2 2045.1 1701.2 112.5 1307.5LPZ124 346.6 173.2 38.9 119.4 2097.9 543.9 2679.2 221 1941.7 LPZ126436.1 185.4 0 142 1762.2 0 2164 283.2 2343.6 LPZ127 455.7 208.6 0.1109.8 1091.7 1925.5 3143.4 345.4 2565.8 LPZ128 421 602.3 25.1 651.14496.3 2712.3 5456.8 1177.7 3875.7 LPZ131 287.1 401.2 0 58 1931.2 422.43510.2 232.2 1275.8 LPZ133 377.8 399.9 141.9 139.5 2396.5 1989.9 4287.5388.9 1119.6 LPZ136 455.2 191.7 103.3 239.9 2289.7 1775 2586.1 322.41429.8 LPZ137 398.1 123.1 0 214.7 2480.2 1118.7 2138.5 411.3 1935.9LPZ138 1987.8 1102.2 11.9 1112.7 4785.6 4242.8 3044.7 1298.3 2786.7LPZ140 401.4 205.9 115 316.7 2737.2 1950.6 1491.6 414.2 2031.4 LPZ141917 621.2 0 726 4938.4 3889.9 3237.4 1109 4165.8 LPZ143 4702.9 1483.9774.8 6791.2 20187.4 6913.4 23175.5 10795.1 26322.7 LPZ144 529.7 392.625.7 303.5 2644.9 1902.4 2380.9 486 2422.7 LPZ145 410.2 206.5 25.4 152.31618 2219.1 2012.8 357.8 2494.3 LPZ146 294.2 152.3 0 125.3 1063.5 1142.11129 215.1 1927.1 LPZ147 366.5 238 0 246.5 2120.4 956.1 1343.4 260.21539.3 LPZ148 390.6 212.9 0 170 2107.1 2195.4 1832.6 1240.9 3839.8LPZ149 1872.8 1139.7 218.5 1748 6204.2 1641.3 5144.7 3526.5 5193.6LPZ150 1958.1 1284.1 645.6 10615.2 29419.9 2976.7 52052.2 21478.741125.9 LPZ151 3477.3 1936.4 155.4 1423.9 5253.1 1925.1 5434.9 2248.35180.5 LPZ152 963.2 481.9 42.2 658.9 4770.8 3607.7 4661.8 1003 4661.9LPZ153 13685.9 27883.7 11205.9 13827.1 24872.9 15412 26204.7 12163.341713.9 LPZ154 621.9 470.5 45.6 381.1 2965 2584.3 2802.2 416.8 2738.4LPZ155 2004.5 1513.1 388.4 1358.9 4265.3 4159.3 4386.4 1022.6 4543.9LPZ157 2978.4 1332.8 407.8 1400.6 4650.6 3692.3 4760.8 1195.9 5002.5LPZ158 12352.4 18933.2 12155.7 9376.4 23120 15280.6 18384 10293.850995.4 LPZ162 3778.2 4069.3 426.4 2285.5 6458.4 3004.6 5794.3 4161.413171.9 LPZ165 1181 805.1 0 756.4 4641.9 2702.4 5464.6 1470 4822.6LPZ166 4624.9 5016.4 0 2508.8 9907.8 1939.3 5655.3 2999.9 5667.6 LPZ1673339 1655.9 319.8 1101.8 4807 4980 4678.8 1968.2 4248.1 LPZ169 787.5556.1 226.4 461.9 2830.2 2225.4 3549.7 684.4 3249 LPZ170 851.1 501.5 0589.6 4405.2 4440.6 4652.4 1622.7 4556.9 LPZ171 1325.6 612.2 0 697.63647.5 3148.7 3446.8 1053.8 4043.4 LPZ172 748.6 490.4 0 802.4 3953.22939.1 3550.9 848.3 3809.5 LPZ173 4460.6 3415.7 1050.1 5769.8 16078.46658.8 16861.6 8623.4 21007.1 LPZ174 501.9 308.6 36.2 358.6 3054.11731.2 1954.9 862.9 2773.9 LPZ175 1476.5 1057.1 181.6 836.9 3731.43120.9 3879.4 755.9 3687.2 LPZ177 302.3 228.3 18.6 107.1 753.6 1430.4941 165.4 1411.2 LPZ179 616.8 314.9 8 379.4 4544.7 2954.4 3425.3 1361.55310.3 LPZ181 1103 430.7 0 671.3 4917.1 3821.3 4976.3 2646.1 5785.8LPZ182 992.8 435.1 0 468.1 3930.1 4177.6 3287.5 909.9 4820.2 LPZ1862455.7 1428.7 760 2414.4 9679 4431.1 5537.9 3859.3 5921.7 LPZ189 40770.368311.4 75133.7 45673 47303.1 21072.6 66542.7 34849.1 78485.4 LPZ194612.8 572.1 155.6 376.3 3673.5 2240.2 3365.2 469.5 3608.6 LPZ195 676.9346.6 32.5 459.9 4278.1 4622.4 4442 1006.8 4754.3 LPZ196 2923.1 1787.1448.6 1537.1 4490.3 3684 4875.6 1426.9 4447.8 LPZ197 592.3 177.5 343.1629.4 5825.4 3639.7 4308.7 649.9 2838.2 LPZ198 801.7 295 8.2 511.1 43843156.4 4823.8 1125.6 3947.2 LPZ199 402.5 165.1 62.7 265.9 2158.3 2363.73531.7 423 2360.1 LPZ201 1338.5 361.2 209.6 1053.7 4997.8 4565.6 5107.62350.9 5882.2 LPZ202 8178.9 2795.3 2359.9 12950.9 39890.9 20247.962085.4 31826.4 78541.6 LPZ203 1983.5 1083.8 1146.8 2044.1 9173.9 2174.811135.5 4073.8 19571.3 LPZ204 38154.8 66241.1 88440.7 45372.1 4716621054.3 65826.6 33875.6 78581.7 LPZ205 1079.5 521.7 589.1 758.4 4742.53837.7 5220.2 1453.2 4888.6 LPZ206 947.6 700.1 505.1 697.8 4089.6 2774.15242.9 1190.3 4578.7 LPZ207 39899.6 60813.3 95637.5 45997 47123.621073.2 63996.4 35149.4 73080.5 LPZ208 27268.6 27230.4 39428.3 2957940216.5 20480.2 42465.8 26526.9 76872.4 LPZ210 683.8 538 429.6 439.82402.5 3285.4 2419.1 636 3965.6 LPZ211 603.1 530.3 87.2 434.6 3653.32234.5 1744.1 359.8 1644.7 LPZ212 1018.4 580.7 155.2 1734.1 9212.32338.9 5061.9 2257.3 4303.3 LPZ213 465.6 327.1 33.1 296.6 3012.3 2419.42198.4 570.9 2013.9 LPZ214 245.6 267.4 0 160.5 912.5 756.5 880.7 133.9936.8 LPZ215 981.1 492.8 52.3 828.3 5390.6 4771.4 4868.1 2893.1 15622.6LPZ216 9410.5 3834.8 1843.2 14492.9 43137.1 21097.9 63778.6 34830.778538.8 LPZ217 31119.9 47126.3 30689.1 28515.4 38695.3 19884.5 37913.921282.9 69590.2 LPZ219 1300.2 886.5 475.3 946.3 4898.3 4361.6 3705.41784.6 5810 LPZ220 5695.5 7233.6 9375 4579.9 6540.8 4340.1 5612.6 2993.65852.3 LPZ221 242.6 209.5 0 186.3 2553.4 2132.5 1632.9 258.2 3202.9LPZ222 186.9 130.5 0 163.4 2138.4 1386.3 1855.4 223.3 2407.6 LPZ223289.9 168.4 0 164 2019 1582.7 2374.4 250.3 2309.2 LPZ224 308.3 114.320.5 333.9 2467.7 2535.2 2722.9 518.9 3054.9 LPZ225 5540.8 1287.2 919.37087.6 18926.6 9277.7 20292.9 8705.5 19974.7 LPZ226 179.1 111 9 136.81049.8 2071.1 3332.8 463.4 2517.8 LPZ227 243.5 136.2 0 341.4 2502.52597.6 4869.5 3226.2 16356.7 LPZ228 470 249.6 0 349.2 4062.6 3388.65688.3 2586.2 15112.1 LPZ231 480.4 296.3 87.4 326.6 3832.4 2343.1 5557.31542.5 5679.2 LPZ233 468.6 332.9 161.7 350.3 2519.3 1967.1 5201.8 996.14833.8 LPZ234 525.8 368.6 287.8 380.3 1626.6 1285.8 4231.8 1235 5329.5LPZ235 310.7 233.3 0 370.2 1746.3 1474.1 4135.8 541.1 3382.9 LPZ237683.5 468.8 487.2 506.9 3683.2 2108.7 3966.8 1369 5254.5 LP2239 546192.6 339.2 331.5 2584.8 2384.8 3215.7 754.4 4041.1 LPZ240 353.7 280.8163.9 274.8 2281.8 1696.4 2345.8 319.2 2180.8 LP2241 283.3 260.2 0 206.42481.1 1787.8 775.1 426 1729.3 LP2242 7478.4 2664.4 1039.1 7024.122393.3 11120.5 20928.9 8581.6 22951.9 LPZ243 242.4 167.1 0 157.2 2175.8182.4 806.9 237.7 977.2 LPZ244 350.3 206.1 0 409.4 4522.7 3997.1 4486.31022.8 3387.1 LPZ246 260.9 200.9 0 251.8 1930.9 1335.1 995.1 1033.74712.9 LPZ247 438.3 274.4 0 341.4 2994 2325.3 2926.7 1508.7 5494.3LPZ248 748.4 714.1 291.3 878.4 3814.2 2737.7 3489 1155.1 4689.5 LPZ249373.3 375.6 0 613.1 4798.1 2088 3560.8 433.5 2486.3 LPZ250 159.5 201.7 0317.6 2037.8 2085.9 1721.8 220.5 2051.7 LPZ251 141.7 157.9 0 178.91377.4 1723.8 1136.8 91 1271.1 LPZ255 220.8 176.1 0 646.5 4160.8 2725.55110.3 1217.2 5217.7 LPZ256 94.6 101.7 0 149.5 821 812.6 1989.5 65.4432.2 LPZ257 147.9 118.4 0 135.1 1206.9 1208.1 2197.2 207.9 745.8 LPZ258168.3 124 0 174.7 2264 2172.4 2672.8 532.8 2245.2 LPZ260 213.5 172.9 0141.3 1172 2974.6 4118.3 232.4 1057.4 LPZ261 147.2 78.5 0 126.6 1212.52349.9 4604.9 139.4 741.6 LPZ264 318.3 174.1 0 201.7 3104.4 2387.65505.8 582.7 3775.1 LPZ265 1566.8 449.9 129.7 646 4992.3 3477.7 5635.5988.7 4307.2 LPZ266 92.8 287.2 0 132.5 931.4 0 4689 190 726.3 LPZ268171.3 217.2 0 206.8 2142 2135.8 5156.3 296.8 1912 LPZ269 1530.1 571.5419.3 2333.1 11130.7 4947.4 13881.5 5155.2 5755.6 LPZ270 162.3 291.4 0450.4 3822 3736.4 4342.8 978.4 2987 LPZ271 454.6 266.6 45.9 381.8 3194.72859.7 3598.7 1277.5 4054 LPZ272 2943.2 763.9 613.2 1451.3 7894.9 2900.85222.6 3222.4 16317.5 LPZ273 215.5 178.7 0 112.2 1288.3 908.3 145.5182.5 544.8 LPZ274 271.5 189.3 0 322.8 3311.7 1141.5 1301.4 620.8 3182.2LPZ275 174.5 152.8 0 99.6 1052.3 0 0 109.3 887.1 LPZ276 146.8 139 0129.4 1165.3 123.5 505.4 82.6 461.9 LPZ277 201.8 137.5 1.8 57.1 761.5931.9 497.5 106.1 1427.3 LPZ278 177.9 152 0 76.6 588.2 1424 311.2 107.31178.8 LPZ279 183.3 179.3 0 304.9 2458 1032 524.7 276 1530.9 LPZ280142.1 125.5 0 125.6 1116.5 623.5 1147.9 125.4 770.5 LPZ281 18 109.8 058.9 563.4 850.5 564.6 11.5 317.8 LPZ282 54.3 164.1 48 95.5 1493.61874.9 1033.3 54.7 844 LPZ283 1607.8 392.6 48.4 1220 6358.1 2922.65552.8 1970.3 5032.3 LPZ284 42.5 119.4 0.3 48 804 748.4 1365.8 66.6 0LPZ286 34.3 164.5 0 74.8 973.6 1463.4 1205.7 81.2 329.3 LPZ287 118.8186.4 0 116.4 1573.7 1568.6 2124 252.9 884.4 LPZ288 103.4 162.8 0 78.31328.1 2890.4 4192.3 196.9 598.1 LP2289 137.1 87 0 113.6 2096 21015147.9 332.3 913.7 LPZ290 1598 425.5 186 1782.8 10543.9 4598.5 16706.43923.8 5648.6 LPZ293 155.8 225.7 0 129.9 3228.2 2291.5 4891.2 316.9 2001LPZ294 65.5 180.6 0 66.6 2237.5 527.7 4397.4 127.5 688.2 LPZ295 119.9258.8 0 149.7 1964.6 560.6 5062.1 141.5 897.3 LPZ297 333.9 277.6 59.4830 5667.2 3950.7 5680.5 1452.6 3614 LPZ299 102.6 225 12.2 268.1 734.7898.2 4025.5 371.2 913 LPZ300 231 271.7 97.1 42.6 113.2 1713.7 3264.54295.2 631.1 LPZ301 272.7 378.2 155.6 97.2 77.2 2110.4 1733.8 485.61392.8 LPZ303 145.6 184.3 641.8 52.8 1562.5 1072.8 365 55.5 358.3 LPZ304422.5 346.5 108.2 350.8 3262.7 2215 1102.8 264.1 1534.7 LPZ306 2207.6484.7 609 3182.2 9671.4 3639.8 17157.3 3556.4 15015.7 LPZ307 1761.11119.4 454.7 846.1 4114.9 2673.3 4082.5 554.8 2983.5 LPZ308 153.5 213.585.5 113.3 1369.9 1433.3 133.8 123.2 1146 LPZ309 132 192 14.3 49.21137.6 1626.7 126.9 85.1 805.1 LPZ310 325.9 353.6 311.3 253.1 4210.42703.3 1846.5 513.3 3102.9 LPZ311 176.9 217.7 72.3 245 3652.2 4352.64175.7 734.7 4625.7 LPZ312 70.4 177.2 139.4 53.2 2094.2 1461.1 945.5 57274 LPZ314 247.5 221.2 217.2 66.9 1767 753.8 872.4 93.1 751.2 LPZ315167.6 220.4 322.5 172.9 3442.4 1985.7 2505.1 698.5 3667.7 LPZ318 912.5297.8 441.9 957.6 7473.1 2682.6 6826.4 2471.8 5148.9 LPZ320 7.3 212.8154.8 55.8 1682.1 1548.5 1038.5 111.1 822.5 LPZ321 199 259.6 157.2 96.82588.8 2465.4 3436.8 409.2 3989 LPS001 369.9 656.8 1322 4095.5 4733.47892.2 3248.2 5064.2 6260.9 LPS003 442.5 392.9 262.1 648.7 2035.7 570.64524.4 1332.8 543.9 LPS004 811.4 552.2 515.2 1694.9 2438.2 425.1 1218.8714.3 86.8 LPS006 271 141.2 200.2 0 662.1 1245.1 261.5 1282.6 405.7LPS007 212.1 91.8 139 47.7 243.4 0 929.2 568 267.1 LPS008 197.6 196.8224.7 0 235.1 2144.5 1034.5 985.2 307.3 LPS010 152.7 133.4 253.8 241299.5 1667.7 242.1 1218.8 1170 LPS011 186 136.1 257.9 816.5 257.4 2372.277.7 1377.7 1346.8 LPS012 317.2 238.4 236.4 817.5 1316.7 1626.1 0 1324.1564.7 LPS013 349.5 403.6 412.9 1840 1582.6 2769.8 215.4 1196 1328.8LPS014 511.4 1574.1 257.3 4169.4 5035.7 4543.5 1016.4 3744.8 3779.2LPS015 2495.9 3076.7 1720.8 7899.3 3640.5 7103 1228.1 5376.7 11989LPS019 1028.2 604.5 239.7 3074 2189.3 2161.1 1239.9 2955 2426.7 LPS020324.1 222.9 120.5 1214.3 1056.8 676.2 872.7 916.8 1058.1 LPS023 160.9116.3 50.3 623.9 1984.1 0 640.7 349.7 694.3 LPS024 359.3 324.3 198.91596 2789.8 1253.1 1212.2 2644.1 3158.3 LPS025 614.7 616.8 493.6 3452.43736.3 3726.5 1796.1 4133.4 5569.5 LPS026 153.7 494.7 0 3053.4 3077.52157.2 806.2 3886.5 2133.1 LPS027 132.2 267.1 0 1309.2 2323.4 1330.21501.6 1931.3 1355.1 LPS028 214.3 446.5 155.7 2472.5 3336.1 2467.92987.9 4507.5 3530.8 LPS029 202.7 384.3 223 2040.4 2060.5 2600.8 7214.84150 3867.7 LPS030 113.5 132.5 0 515.6 2793.9 110 2158.7 2684.7 1237LPS031 168.7 123.5 11 557.6 3086 812.5 6212.6 2534.1 3290.9 LPS032 145.2160.9 1.7 650.5 2144.2 1070.8 1474.2 2819 3561.4 LPS036 582.6 616.4200.9 2224.2 2656.4 1723.6 3073.1 1866.1 2955.4 LPS037 502.6 620.4 2191626.6 3359.2 2705.7 2125.3 2456.9 2004.1 LPS038 962.6 216.5 375.7 01256.5 930.9 1492.9 1578.3 1406.9 LPS040 228.9 86.1 158.6 0 256.8 0245.1 758.3 1.7 LPS041 222.7 149.2 123.3 0 252.4 0 965.1 1065.9 553.4LPS042 447 661.1 489.9 1672.8 2520.7 492 1046.8 2834.7 1940 LPS043 333.7264.6 407.3 656.8 1046.4 546.5 1412.3 1097.9 948.4 LPS044 249.8 277.8652 1327.3 907.5 1110.5 1892.3 1353.4 1674.8 LPS045 250.7 107.2 177.9302.6 231.9 944.7 1881.7 0 485.4 LPS046 232.4 285.6 224.5 1302.1 1872.61104.6 2610.7 1128 1744.2 LPS047 2649.2 6969.7 2792.6 14436.1 101415428.8 4057.3 2999.2 13647.7 LPS050 2428 7502 3442.1 12204.8 7385.49850.6 3395 8052.4 14726 LPS051 478.2 219.7 175.1 1861 2222.1 1876.91922.5 800.9 2052.9 LPS052 328.2 196.2 138.3 984.6 1458.3 1324.4 15851112.9 1726 LPS053 264.2 111 0 1370.2 1524.9 1548 2692.1 2941.5 3096.1LPS054 285.4 231.1 8.5 1370.4 2138 1273.8 2930.8 2762.2 2604.2 LPS0551192.2 1118.8 180.5 5840.8 4203.1 3690.8 1865.1 3205.9 4824.3 LPS0561541.6 2959.9 1354 9519.2 8084 6331.3 1820.6 5902.8 11668.9 LPS057 214.5406.9 0 2028.8 2744.4 756.5 2136.1 1844.8 2454.6 LPS058 277.4 244.5 70.81623.9 1826.1 1626.5 2708 2514.4 4077 LPS059 115.3 135.2 1.5 996.91194.7 1022.8 1723.1 1265.3 2390.7 LPS060 163.3 268.5 0 1866.5 1707.31953.7 2184.7 2422.6 2990 LPS061 222.7 255.6 53.4 1448.5 2146.6 1600.71956.2 2511.7 3332.5 LPS062 136 228.5 99.1 627.1 863.2 467.7 1610.32304.9 2842.2 LPS063 299.7 251.8 226.3 796.8 1427 1771.5 1174.1 9301433.9 LPS064 3079.8 4014.9 3039.3 7349.2 10807.8 7372.1 10515.8 4426.813038.7 LPS065 434.1 644.6 313.7 1147 1456.3 3097.5 2632.9 3695.2 1575.1LPS066 214.7 171.7 134.2 0 69.3 249.7 726.1 871.9 586.6 LPS067 706.6686.2 488.8 3013.5 2498.9 4522.1 4844.3 4782.1 5775 LPS069 199.6 172.1123.9 75.4 19.4 269 874.1 854.6 0 LPS070 143.6 186.9 117.9 289.4 685.5222.6 528.3 582.6 322.6 LPS071 180.4 170.2 187.2 157.2 183 882.3 326.7508.4 310.2 LPS072 235.9 170.8 169.6 449.9 290.5 777.5 456.3 283.3 479.2LPS073 900.4 1318.7 629.3 3416.1 4420.4 3894 4010.1 3367.3 4106.8 LPS07427858.2 33812.3 32162.2 44513.2 111430.2 87262.8 47575.8 18233.4 66903.6LPS075 2119.6 3296.9 1347.3 9540.3 5518.1 6367.8 10437.2 4054.1 9821.4LPS076 347.6 336.3 218.5 2343.7 2326.5 1569 2415.2 1580.7 1990.4 LPS077568.1 612.4 550.1 2908.9 1727.9 1660.2 2164.5 1798.6 2588.1 LPS0783174.9 3137.6 3222.6 7616.1 6945.1 9024.5 11397.6 7995.6 26362.3 LPS0791049 1066.4 302.2 4400.8 4126.8 4404.7 8203.7 4645.6 9377 LPS080 21208.728180.7 9065.4 39068.1 63741.1 37523.8 35948.5 11444.8 57266.6 LPS08127381 33419.5 10292.3 43529.2 63629.5 28119 42128.9 15984.6 62043.4LPS083 711.4 825 64.8 3306.9 3733.1 1898.2 3688.2 3407.5 3959.9 LPS084216.4 211.2 21 1350.5 1724.2 1394.5 1965.6 2089.4 3604.5 LPS086 185.6214.1 0 1808.2 1476 2915.6 2342.5 932.2 3339.1 LPS087 3404.7 5840.34144.1 12101.2 12860.9 14601.5 24953 5018.8 19643.5 LPS088 165.2 224.662.5 1497.7 2813.8 1593.8 3740.9 4017.3 3934.9 LPS089 223.8 213.7 01318.9 1574.5 2141.4 2443.5 3799.4 4185.6 LPS090 398.7 693.2 142.22593.7 2695.2 3465 3755.9 3638.7 3587.8 LPS091 391.2 700.6 270.7 1469.22092.2 3047.2 3754.2 3524.1 3149.7 LPS092 286.1 376.1 254.9 235.2 433.11353.8 1747.8 2658.1 3246.6 LPS093 185.6 273.7 126.3 114.7 296.8 254.7412.1 1076.9 483.7 LPS094 232.8 261.8 151.7 274.3 249.9 641.1 891 5771102.1 LPS095 199.3 191.7 91.4 25.7 169.9 433.7 813.4 1394.3 807.7LPS096 97.9 139.2 63.2 0 162.4 159.2 504.6 752.3 150.6 LPZ001 150.8207.8 257.7 202.6 485.5 704.2 555.2 1924.3 528.5 LPZ002 154.6 167.7323.6 937.4 717.4 755.6 1068.8 1089.8 1107.7 LPZ003 1348.1 1781.1 609.35019 4261.6 6148.5 5600.4 3419.6 4858.4 LPZ004 2798.4 5533.1 2921.810703.8 8020.5 9958.4 10424.8 4135.9 14742.8 LPZ005 8403.4 19547.78589.6 32699.4 31426.9 21580.2 19437.9 8059.6 14660.3 LPZ006 360.62106.1 1173.8 7812.9 7966 11310.2 10516.8 4661.6 4618.2 LPZ007 272.5409.9 454.7 2038.3 1107.6 2043.7 2073.5 2249.9 2751.3 LPZ008 207.7 258.2212 1939.1 1482.6 1926.1 2243.5 1036 3324.9 LPZ009 745.1 496.5 633.94276.8 7474.5 9130.4 9814 5721.3 14116.8 LPZ010 893.7 1464.1 326.63759.5 4034 4261 4672.2 4388.6 9625.3 LPZ011 1829.5 2488.7 350.4 5922.74285.4 2984.2 5579.8 4236 9972.7 LPZ012 227.8 289.9 28.6 1885.9 1660.5843.6 1913 1434.5 2785.5 LPZ013 247.7 213.8 84 1553 2015.3 1547.3 2567.63196.5 4347.7 LPZ015 261.7 315.8 55.6 2254.3 2409.8 2190.1 2562.4 1291.23237.3 LPZ016 2750.3 2151.8 3003.8 8316.2 6689.1 9147.4 9444.8 3349.68167.8 LPZ017 582.2 701.3 227.4 3830.1 3650.3 3828.2 4552.3 4574.54476.8 LPZ018 2867.6 6184.2 2746.8 10513.4 9443.1 10880.7 12748.3 5491.219422 LPZ019 7551.3 15875.3 9232.8 20440.4 22870.4 31026.1 33842 12823.638075.4 LPZ020 293.1 896.2 143.1 1661.8 2519.9 2987.1 4132.4 4022 3145.1LPZ022 213.3 493.5 173.5 82.5 467.8 1355.1 1041.1 1481.9 1035.5 LPZ023191.2 616.7 118.1 78.4 184 955.2 516.3 1254.6 574.4 LPZ024 142.6 321.7118 0 81.9 826.8 195.8 763.9 491.7 LPZ025 661.9 764.4 536.9 1885.61791.8 3200.6 3017.8 4028.4 3897.8 LPZ026 194.7 221 150.4 1102 513.61714.4 1291.2 1625.6 980.7 LPZ028 301.3 424.2 210.9 1467.7 1654.3 2848.41937.1 4092.7 3086.2 LPZ029 132 151 124.8 319.5 644.1 478.7 452.4 564.8709.3 LPZ030 305.3 616.6 170.6 2800.7 2572 2485.4 1960.4 1425.4 2381.1LPZ031 1945 3098.6 3636.7 12422.3 7673.2 8643.5 11552.7 4295.4 4600LPZ032 26761.5 33518.4 33623.2 45482.1 106536.1 114284.5 46968.1 16371.642282.1 LPZ033 2068.3 1779.5 6651.8 7887.8 5249.8 9848.7 7632.1 4500.77710 LPZ034 221.4 363.3 216.3 2503 1949.2 1674 2078.9 1428.3 1774.9LPZ035 110.7 156.8 85.2 836.6 512.1 1355.7 1217.4 294 1525.3 LPZ037229.5 206.2 186.5 1422.1 1962.7 2742.9 3023 614.1 2895.5 LPZ038 605.7722.8 352.5 3551.8 3072.2 3614.2 3266 2494.6 4039.7 LPZ039 366.4 964.6177.1 3755.2 2744.9 4599.4 3589.7 2407.8 3925.2 LPZ040 185.6 278.3 100.82131.2 1321 1479.7 1654.6 773.7 2087 LPZ041 119.9 120.8 5 1199.8 1220.81090 1431.2 630 2206.1 LPZ042 61.9 121.9 2.6 731.4 1897.5 986.4 1366.1458.4 2625 LPZ043 357.9 355.1 0 3236.5 2746.8 2960.1 3138.2 911.1 3345.6LPZ045 738.6 1003.7 565.3 3866 3168.1 6406.2 4028.7 4526.2 4573.4 LPZ047139.7 133.1 0 481.3 857.9 831.8 954.1 1926.3 3129.6 LPZ049 1396.5 2125.51496.9 4514.4 3629.8 5942.4 6898.6 3610.7 9214.2 LPZ051 264.6 610.7205.1 826.3 1819.9 2243.8 3000.9 3400.7 2810.3 LPZ053 174.9 827.9 152161.2 563.8 1149.7 1277.9 1243 1383.5 LPZ054 205.7 951 128.4 976.11901.4 1626.6 1265.8 1437.6 1328.8 LPZ055 135.2 389 168.5 420.2 524.31650.6 848.4 1200.6 914.2 LPZ056 190.2 323.3 229.5 439.9 664.2 1613.11014.2 1727.3 1126.2 LPZ057 87 199.9 180.1 2154 863.7 3059.1 2994.92696.3 2990 LPZ058 139.3 227.5 55.3 1695.1 902.5 2426.6 2195.6 1925.41598.2 LPZ059 173.4 289.6 189.4 891.7 759.7 1835.3 1332.9 962.5 1286.6LPZ060 301 464.8 114.1 2296.5 2860.4 2786.6 2974.4 1629.6 2301.9 LPZ0611212.2 1711.1 794.4 7468.5 5190.1 7957.5 5857.5 2819.8 3922.3 LPZ0622078.9 3648.3 2499.3 14932.7 7691.9 9294.1 9213.3 3077.8 4850.3 LPZ063641.6 984 2114.2 5547.2 3688.6 6191.3 4844.5 4058.7 4162.4 LPZ065 520.2443.3 332.3 2720.4 1816 2848.5 3320.4 4501 3948.4 LPZ066 663.7 357 356.63458.6 2196.3 3567.6 3081.4 1325 2388 LPZ067 1469.5 2582.6 3152.4 8674.98080.1 9367.6 8556.2 4135 7240 LPZ069 211.4 283.6 0 1921.6 913.1 1567.51866.5 1043.3 2269.1 LPZ070 229.6 334.9 2.8 1659.3 1254.6 1681.6 1883.61360.7 2442 LPZ071 332.3 633 15.7 3126 2729.9 3290.2 2998 2011.7 2744.8LPZ072 39 38.9 0 581.2 1401.7 1307.1 1089.7 710.4 1866.3 LPZ073 131.3250.9 4.4 1176.4 2903.2 2356.7 1718.3 985.9 2700 LPZ074 92 116.5 0 355.61643 1041.3 1027.4 1042 2553.6 LPZ075 195.9 0 0 0 474.7 488.1 847.61488.4 2755 LPZ076 232.4 134.5 730.7 0 268.9 0 568.3 1007.4 2624.4LPZ077 1143.1 2350.7 187.5 6551.1 5960.9 5520.2 7189.6 3483.6 7931.5LPZ078 851.5 1021 873.3 3011.7 4619.7 5273.6 6408.3 3950.1 8831 LPZ079281.6 779.7 315.9 1296.6 2065 2090.8 2287 2271.9 1824.5 LPZ080 1653.53124.5 3778.7 8321.1 7987.7 10470.3 8085.7 4454.9 8067.4 LPZ081 92.6292.7 161.8 746.8 903 1558.5 1410.8 746.1 1230 LPZ082 123.2 430.5 240.11907.9 1283.9 2707.5 1801.7 948.9 1634 LPZ083 77.8 183.2 120.2 40102437.2 4390.6 3649.7 3983.1 7032.5 LPZ084 1272.3 956.1 1297.2 58814505.3 10642.2 9798.6 3938.5 8446.3 LPZ085 321 466.2 457.6 4324.8 4008.46890.5 4599.7 5672 10082.2 LPZ086 1529.6 3587.2 3236.8 10729.4 10010.210739.2 10634.9 4674.2 11980.7 LPZ089 614.3 500.4 601.9 4196.2 3890.54405.8 4331.9 3066.9 3960.3 LPZ090 643.8 1177.5 1315 4017.8 4456.26394.5 4824.4 3000.1 3538.3 LPZ091 1006.7 1754.2 4090 11615.8 11728.516837.8 14461.9 5005.1 14726.4 LPZ092 419 528.6 1336.8 4158.4 3568.18393.4 8192 4638.4 3939.4 LPZ093 162.2 453 90.9 1436.5 899.2 2218.6 1798741.8 1789.8 LPZ094 123.1 197.1 0 1355.7 779.4 1360.5 1713.8 794.41597.8 LPZ095 94 130.6 3.5 1099.9 737.4 558.3 1473.4 871.4 1809.3 LPZ096314.3 327.5 22.1 2574.2 1620.9 2748.1 2533.9 1858.4 2979.9 LPZ099 231.7359.8 6.2 1456.9 1335.7 1672.4 2170.8 2160.8 2755.8 LPZ100 375.6 650.4136.7 2834 2518.6 3053.2 3159.8 2841.2 3453.7 LPZ101 217.1 425.7 11.82769.3 3312.6 2556.3 5262.5 1501.2 2947.3 LPZ102 294.3 289.2 55 1803.62764.5 2532.5 2613.4 2447.6 3377.6 LPZ103 224.2 92.8 17 989 1850.61643.7 2303.4 3729.5 4029.5 LPZ106 328.4 158 57.5 912.5 1239.6 1214.51669.6 2062.3 4720 LPZ107 25137.4 28865.6 19438.9 43316.7 75674.158296.4 45927 3332.1 64391.6 LPZ108 1132.8 2084.1 330.6 4299.2 3629.84480 6406.5 3235.2 9993.6 LPZ109 548.8 1356.1 417.7 3237.9 2581.9 3177.53977.1 1874.2 4107.7 LPZ110 379.7 1132.3 220 3941.9 2720.7 4103.6 35632245.7 3031 LPZ111 157.5 383.2 200.3 1188.3 749.8 1675.7 1926.1 1685.11655.3 LPZ112 332.1 522.9 220.7 2950.9 2223.9 2257.5 2856.4 2632.82711.8 LPZ114 590 151.8 217 4659.6 3824.5 6848.2 7228.9 2831.3 9446.9LPZ115 6268.6 3368.3 8724.6 25695 19481.5 33011.8 32267.9 2903.4 52760.4LPZ116 891.2 766.7 1481 6455.1 3684.7 5597.5 8456.7 1682.9 10139.2LPZ117 529.8 826.6 126.5 3706.8 2663 2897.5 3996.1 1807.3 2635.2 LPZ118437.8 628.1 122.1 3903.5 3245.2 3529.9 3500.1 3506.1 2867.9 LPZ119 331.3755.1 83.1 3761.2 4072.6 4024.9 3449.9 3742.2 2533.9 LPZ120 196.5 796181.4 4219.4 3297.7 4322 4539.3 2622.7 3763.6 LPZ122 154.4 208.4 1582332.3 1468 3044 2838.8 2112.5 2323.6 LPZ124 172.9 420.1 136.6 2319.51539.3 1722.7 2453.6 3109.1 2297 LPZ126 446.5 604.2 187.3 3859.4 3120.82816.9 3324 2181.4 3237.7 LPZ127 439.3 499.4 53.5 3781.8 3083.3 3769.83848.4 1729.6 2902.2 LPZ128 1022.3 1063.8 447.9 3904.9 3753.5 5579.64534.2 2133.1 5843.6 LPZ131 249.6 373.8 27.3 2170.1 1551.6 1611.4 3077.32946 2409.5 LPZ133 325.3 328.7 42 2706.9 2358.1 2131.3 3257.2 2598.72144.3 LPZ136 263.6 384.1 0.9 2377.3 3386.4 2044.7 2726.4 2398.2 2570.6LPZ137 351.6 402.6 91.9 3435.9 3122.8 2406.9 3054.7 3392.1 3216.6 LPZ1381047.7 936 530.3 3920 3091.2 2507.9 4249.5 3109.9 4758.4 LPZ140 379.9456.6 105.6 1936.7 3674 2908.4 3678.1 3663.2 4481.7 LPZ141 715.4 1341.8536.4 4712.8 3615.6 3746 4815.5 4533 8229.3 LPZ143 3251.1 4810.8 2848.59675.1 8289.6 9486.6 12270.6 4424.9 28251.9 LPZ144 386.2 1338.3 294.13433.8 3015.7 3333.7 4618.9 3699.7 6825.1 LPZ145 277.5 1064 536.5 3072.31155.6 2977.7 3188.2 7388.2 3013.6 LPZ146 128.5 362.8 139.5 2224.4 13642125.6 2428.3 3465.8 2351.1 LPZ147 266.5 544.2 241.3 3318.1 2241.92162.6 2908.8 3252.6 2525.6 LPZ148 725.8 725.5 290.2 5002.2 3682.16542.3 6927.9 2903.1 10410.9 LPZ149 2492.7 3102.7 1052.7 8980.9 7669.27185.3 9921 7375.4 12848.8 LP2150 5257.3 7586.3 3221.3 15156.2 12776.87401.2 14686.5 1570 20635.9 LPZ151 1384 2049.2 1128.3 5576.7 3853.73780.3 7227.3 3206.7 7702.7 LPZ152 975.8 1300.2 546.4 5096.1 4344.94302.5 4960.3 1566.2 4223.4 LPZ153 13378.6 22235.1 10073.1 33495.150802.7 28291.5 29602.5 3224.1 40232.7 LPZ154 663.3 738 430.3 3847.33880.2 6253.9 4707.7 5341.9 3393.8 LPZ155 1121.7 612.7 748 3902.3 42894676 5328 6964.2 5353.5 LPZ157 1157 957.9 762.2 4218.8 4266.6 3777.24583.7 5017.5 4396.6 LPZ158 13278.5 17582.9 9898.7 32456.5 42805.124534.6 31442.6 4152.5 36015.3 LPZ162 3407.5 5943.1 5126.6 10324 11710.97727.6 7572.2 2312.6 9394.1 LPZ165 1419.4 1550 693 4519.9 3692.9 6102.15617.1 3061.3 4706.4 LPZ166 2642.1 3504.9 1288.6 7134.8 6994.5 5170.510453.3 4932.9 15993.7 LPZ167 980.4 1518.1 564.7 4902.6 4803.7 3343.64869.5 4421.2 6582.2 LPZ169 621.8 792.7 93.4 3562 4266.3 1794.9 3496.34238 3143.7 LPZ170 1009.3 1405.3 695.2 5793.7 4469.2 4952.7 6239.73914.1 6782.3 LPZ171 1064.6 968.6 1050.7 4110.8 3903.7 5467.2 56593164.1 6795.3 LPZ172 1233.3 1113.3 401.8 4207.9 7922 8417.5 10419.27983.5 15065.2 LPZ173 3333.8 5236.8 6072 9552 8880 8653.2 13461.6 2408.725719.1 LPZ174 486.7 1263.6 143.3 3318.2 2027 3632.3 4245.3 6086.48781.2 LPZ175 594.7 1487 520.8 3051.5 3610.8 1846.4 3642.9 4048.5 4329.5LPZ177 167.3 481.7 234.5 1955.9 1139.2 907.8 1452.9 4462.5 1762 LPZ1791231.4 1583.2 835.8 5029.7 3654 3871.7 3248.8 2741.9 2853.2 LPZ1812259.5 4282.9 774.8 8911.6 8788.6 6833.3 5825.5 3398 3922.8 LPZ1821194.7 2289.6 673.8 8220.3 5601.2 5869.3 5572 1808.4 7607.9 LPZ1866255.6 6866.5 6841.1 24861.8 16742.4 23502.2 17304.6 1750.4 27328.9LPZ189 27581.8 34787.4 31889 45673.7 106688 95043.9 46998.5 3548.167943.9 LPZ194 673.9 904.9 528.7 4064.5 3530.2 3160.6 5095.2 4531.13492.1 LPZ195 898.9 889.6 675.7 5606.3 4004.3 5230.5 5721.4 4908.14940.9 LPZ196 1073.7 1935.1 558.2 3941.1 3672.6 3681.6 5977.8 3059.75050.3 LPZ197 488.4 522.5 386.6 2613.4 1684.4 3541.4 3507.4 4980.82763.1 LPZ198 575.6 733.1 299.6 4152.3 2411 2916.8 3872.4 6530.7 2931.8LPZ199 390.7 442.5 222.4 2704 2176.2 3159.7 2957.1 5357.6 2983.4 LPZ2012255.7 3876.6 347.6 10222.9 6897.7 6294.3 7324.7 3549 3857 LPZ20225939.2 34864.8 28937 43395.9 85136 71116.8 38688.5 2676.2 14449.1LPZ203 4917.3 4458.1 2603.2 10348.3 6571.1 8325.7 11034 5453.1 6598LPZ204 27637.2 31853.9 22475.7 43983.9 89520.6 46504.2 44333.5 7963.858054.1 LPZ205 1184 1084.2 327.7 3901.8 4402 3125 4598.2 4501.5 4714.2LPZ206 1309.8 1509.5 367.3 3961.5 3983.3 3079.6 4196.2 3641.4 3247.6LPZ207 27569.1 30446.2 27094.6 45211.6 90196.1 58153.8 46488.1 9879.364709.2 LPZ208 22722 28208.9 30019.5 40314 74354.1 37339 33919.6 3989.856683.6 LPZ210 1015.1 1789.8 196.8 5006.3 6159.7 3067.8 4944 4347.56846.4 LPZ211 277 327.3 519.4 2540.7 1788.5 3048.6 1110.8 2577.4 3506.6LPZ212 1095.5 865.5 930.6 4051.6 4491.6 2857.6 6348.1 4078.7 16174.9LPZ213 376.6 539.3 339.6 2503.1 1540.8 1333.7 3082.2 6024.3 4163.1LPZ214 137.5 306.1 190.1 1915.6 866.1 1280.9 1240.8 6372.4 2111.8 LPZ2153519.4 3120.9 3300.9 16939.5 15489.1 10948.6 12502 3515.1 16236.4 LPZ21626761.3 34226.4 28477.9 42274.8 67630.3 41420.3 36331.4 1433.8 17109.4LPZ217 15563.1 21739.4 12259 26824.9 34266.3 9429.4 28156.7 1339.241568.4 LPZ219 2404.9 3704.5 2084 8575.1 8573.2 6237 11757 3255.413484.9 LPZ220 3617.2 6998.6 7957.2 13960 9400.8 3432.3 10805.5 3551.712867.4 LPZ221 482.6 478 1405.6 3296.5 3079.8 3312.5 4143 4429.4 3267.3LPZ222 318.3 524.5 406.6 3011.7 2309 3811.8 4199.4 5319.9 3292.3 LP2223367.7 633.2 437.7 2752 1970.3 3767.4 2514.2 3672.3 2163.1 LP2224 337.91030.2 317.2 2701.2 1798.7 7393.6 2962.5 7876 3353.7 LPZ225 3288.13590.3 2912 8781.8 7400.7 2317.1 11370.2 3186.6 22244.6 LP2226 325.6361.4 128 2467 1263.7 10190.1 1636.3 3808.8 1166.9 LP2227 2175.5 6375.8458.8 6316.2 6632.1 9013.9 6614.3 3901.2 2588.2 LPZ228 2638 3701.7 500.15991 4819.8 5747.8 7102.5 3182.5 4185.4 LPZ231 1631.7 2090.4 260.75811.5 4749 2530.8 5033.1 3191.3 3810.5 LPZ233 1596.6 1223 296.6 4355.63818.9 2988.8 3749 3324.7 3855.2 LPZ234 1734.3 1479.2 219.6 5058.54614.4 2034.9 4992.1 1979.9 5152 LPZ235 626 635.9 185.9 4066.8 3255.54035.7 3368.7 2880.8 3643.5 LPZ237 1677.8 1385.3 847.4 4536 3702.82943.6 4886.5 2307.8 5136.8 LPZ239 673.4 407.8 245.8 2981.9 3199.22781.6 4235.6 2342.6 4863.7 LPZ240 387 247.4 254.8 2075.8 2317.4 28942721.7 2054.5 4317.9 LPZ241 258.3 337.8 110.9 3503.1 3829.6 22593.51889.5 1315.9 8842.6 LPZ242 4315.9 2560.2 22.5 12510.2 12605.3 2345.216197.1 1114.8 39684.4 LPZ243 174.8 274.4 23.1 2193.6 346.2 2395.71366.5 2568.2 3103.8 LPZ244 417.5 269.1 3458.5 3545.1 1831 2834.7 1781.17589.2 5662.7 LPZ246 889.5 918.7 2302.7 3920.3 3228.9 4409.3 3536 1258.82645.3 LPZ247 1203 2088.9 46.7 4956.9 4253.2 3559.7 4570.8 1702.6 3350.6LPZ248 973.3 1338.1 86.2 3977.4 4392.8 2033 4094.9 2062.6 4279.9 LPZ249361.3 324.3 206.7 1948.6 1764.3 2098.7 2762.1 1643 2862.4 LPZ250 267.6487.8 118.7 2690.4 1522 2989 3121 1928.9 1809.7 LPZ251 245 279.7 168.51409.9 555 9932.3 2552.5 3050.3 1371.1 LPZ255 2021.3 2488.5 334.1 7289.57773.7 1269.1 9020.3 4492.4 10134.6 LPZ256 67.1 72.2 296.7 412.2 229.8922.7 570.3 5040.6 1263.9 LPZ257 167.3 146.9 482.6 521.8 102.5 2699.4599.8 2362.1 1553.4 LPZ258 247.5 236.5 69.7 1429.8 974.6 971.9 2668.72990.8 3445.9 LPZ260 98.1 188.8 463.1 377.1 337.2 880 808.9 1552 1084.6LPZ261 73.7 20.5 386.3 1143.6 50.3 4443.8 903.3 2309 1341.6 LPZ264 482.7528.5 1151.3 3659.7 1972.6 9892.4 2831.4 1584.4 3208 LPZ265 534.6 647457.6 4473 4089.9 656 5899.6 2972.2 5649.8 LPZ266 16.9 61.2 1062.3 876.11183.4 1624.3 663.3 622.4 1609.7 LPZ268 143.7 142.9 255 1983.5 810.39809.8 1293.5 1757.3 2177 LPZ269 1747.5 1271.8 1636.4 7364.1 5108.76903.3 11401.2 3774.1 14643.8 LPZ270 373.8 77.9 1901.2 5015 3872.63485.9 5621.1 4284.8 5197.8 LPZ271 705 473.1 315.6 2863.8 2625.4 2120.54048.2 1424.9 4291.1 LPZ272 2809.4 2423.8 300 5056.2 2463 3534.6 3496.9609.7 3996.8 LPZ273 219.8 162.4 242.4 90.2 130.2 3251.3 166 1193.51836.5 LPZ274 489.2 367.7 284.6 991 1104.2 395.9 2282.3 747.2 4535.7LPZ275 93.5 140 156.8 433.3 217.1 0 837.9 1056.2 2352.3 LPZ276 53 109.7106.8 0 0 0 369.1 1303.6 1897.6 LPZ277 105.9 159.4 68.7 0 0 230.1 236.2706.1 1337.9 LPZ278 65.7 48.3 156.4 0 0 1788.2 406.3 1442.3 1564.1LPZ279 214.7 212.2 75.3 1356.3 790.8 5213.3 1722.4 496 2426.2 LPZ280156.2 247.7 1553.6 3510.5 2515.1 289.7 3182.1 612.7 3123.7 LPZ281 34.692.1 73 0 0 1648.3 565.5 522.8 543.3 LPZ282 200.9 187.7 218.1 536.6205.9 7324.8 1145.4 2977.5 957.5 LPZ283 1833.5 1880 775.8 3303.2 5113.2527.6 5281.2 324.5 3806.8 LPZ284 215 0.6 148.8 0 29.6 848 408.4 148.81294.3 LPZ286 219.7 21.9 13.6 234.9 78.6 2703.6 198.4 947.9 1233.2LPZ287 112.6 126.2 36.5 1170.3 459.9 306.3 157.5 1173.7 1821.3 LPZ28823.6 62.2 37.5 774.1 639.3 792.6 715.8 1422.6 1169 LPZ289 44.1 13.1107.4 323.4 95 9975.4 889.5 2240.6 1894.9 LPZ290 1324.7 1572.4 18386941.6 4616.9 2995.3 11538.8 407.1 12699.5 LP2293 45.2 246.3 145.62785.5 1923.1 0 3185.6 0 3550.4 LPZ294 0 19.8 0 403.3 280.4 89.1 785.9551.6 1378.4 LPZ295 40 24.5 0 169.9 26 1324.9 1058 848.8 1406.5 LPZ297385.6 127.6 17.4 1238.5 941.5 0 2680.9 2084.3 4065.3 LPZ299 106.9 36.2 00 926.2 0 1060.7 1854.9 1575.9 LPZ300 73.2 93.2 80.2 0 1143.6 1053.31034.5 2304.9 2120.8 LPZ301 126.2 0 5.8 161.2 1245.7 516.3 1612 761.32826.1 LPZ303 83.1 488.8 98.6 0 73.5 979.9 538.7 510.7 1214.7 LPZ304213.7 498.3 137.6 1028.6 0 5405.8 860 2212.1 2201 LPZ306 1439.4 1735.32526.4 4212.7 3140.4 2090.1 8128.5 4874.6 14413.9 LPZ307 534.1 710.5515.5 2785.3 734 0 2137.3 1692.8 3540.3 LPZ308 116 304.4 137.7 151.828.2 364.2 621.1 631.4 851.2 LPZ309 80.1 137.2 92.7 0 0 2648.1 529.4192.6 735 LPZ310 430.8 584.9 799.2 1887.2 1887.1 6161.2 2974.3 35752426.6 LPZ311 690.5 995.7 208.4 3725.8 2843.8 0 4329.3 3620.8 4170.1LPZ312 109.8 334.2 34 72.5 4.5 1489.3 140.1 431.6 744.8 LPZ314 26.5200.1 3.3 181.2 0 1231.5 331.5 440.1 804.6 LPZ315 305.8 211.3 147.5811.2 1008.1 3797 2231.8 1438.8 1881.8 LPZ318 621.3 715 337 3488.22480.9 781.9 4326.1 4824.7 6969.2 LPZ320 214.8 92.2 9.9 1170.9 54.54501.5 1122.3 1169.4 1696.6 LPZ321 880.4 755.2 1899.3 6166.2 5105.8411.6 6096.5 4853.6 6057.2

TABLE III LSC Media Multiplication Media Maturation Media Components(mg/L) 16 1133 923 NH₄NO₃ 603.8 603.8 200.0 KNO₃ 909.9 909.9 454.95KH₂PO₄ 136.1 136.1 136.1 Ca(NO₃)₂.4H₂O 236.2 236.2 59.05 MgSO₄.7H₂O246.5 246.5 246.5 Mg(NO₃)₂.6H₂O 256.5 256.5 256.5 MgCl₂.6 H₂O 101.7101.7 101.7 Kl 4.15 4.15 4.15 H₃BO₃ 15.5 15.5 7.75 MnSO₄.H₂O 10.5 10.510.5 ZnSO₄.7 H₂O 14.4 14.4 14.4 NaMoO₄.2 H₂O 0.125 0.125 0.125 CuSO₄.5H₂O 0.125 0.125 0.125 CoCl₂.6 H₂O 0.125 0.125 0.125 FeSo₄.7 H₂O 6.956.95 41.7 Na₂EDTA 9.33 9.33 55.9 Sucrose 30,000 30,000 — Maltose — —20,000 myo-Inositol 1,000 1,000 100 Casamino acids 500 500 500L-Glutamine 450 450 450 Thiamine.HCl 1.0 1.0 1.0 Pyridoxine.HCl 0.5 0.50.5 Nicotinic acid 0.5 0.5 0.5 Glycine 2.0 2.0 2.0 2,4-D 1.1 1.1 — BAP0.45 0.45 — Kinetin 0.43 0.43 — Polyethylene glycol — — 130,000 ABA —5.2 5.2 Gelrite 2,500* 2,500* 2,500 pH 5.7 5.7 5.7*For solid media only

TABLE IV Description of clones used in hybridization study shown in FIG.9. ID with Clone # Homology Description Arabidopsis Score E-valuePC04B12 Lotan et al.. 1998. Arabidopsis Required for embryo 79% ID, 1717e−44 (‘LEC’ in LEAFY COTYLEDON 1 is maturation & Cotyledon 93% + vefigure) sufficient to Induce Embryo identity. Ectopic over 96aaDevelopment in Vegetative expression induces Cells. Cell 93: 1195-1205embryonic differentiation traits in transgenic seedlings. ST17B05PICLKE/CDH3, Chromatin The pickle mutants 50% ID, 166 1e−41 (‘PLK’ inremodelling. Ogas et al. 1999. express embryonic traits 74% + ve figure)PICKLE is a CHD3 chromatin- after germination. over 155aa remodelingfactor that Represses lec regulates the transition from expressionembryonic to vegetative development in Arabidopsis. PNAS. 96(24):13839-13844 PC08C06 FIE, fertilization-independent Fie mutants initiate61% ID 92 8e−20 (‘FIE’ in endosperm protein. Ohad, et al endospermdevelopment 75% + ve figure.) 1999. Mutations in FIE, a WD w/ofertilization over 67aa polycomb group gene, allow endosperm developmentwithout fertilization. Plant Cell 11 (3), 407-416

TABLE V 488 499 499 500 500 (Liquid (Liquid (Liquid (Liquid (Liquid)Cell Line Suspen- Suspen- Suspen- Suspen- Suspen- (Stage of sion sionsion sion sion Develop- Culture: Culture: Culture: Culture: Culture: 260260 ment) Stage 1-3) Stage 1-3) Stage 1-3) Stage 1-3) Stage 1-3 (Stage7) (Stage 9) Media 1133 16 1133 16 1133 Maturation maturation # Embyros

118.5

187.75

Na na ‘FIE’ ++++ + +++ +++ +++ +++ +++ ‘LEC’

++

++

+ + ‘PKL’ ++++ + +++ +++ +++ +++Table 5. Table of data from Fig. 9a & b. Numbers (488, 499, 500, 260)refer to different cell lines Liquid Suspension Culture containsearly-stage embryos (stage 1-3) Embryo number refers to the number oflate-stage (stage 8-9) embryos# produced by each cell line when matured according to Pullman and Webb(1994). + = low expression, ++ medium level of mRNA, +++ = high level ofmRNA, ++++ = very high level of mRNA. Circles # around certain + signs,see text. Na = not applicable. Levels of mRNA are relative and refer tothe experiment depicted in Fig. 9a & b.

1. A relational database comprising the data of Table I.
 2. A method ofstaging embryos comprising: a) providing at least one embryo; b)detecting the expression in the embryo of at least one RNA transcript ofTable I; and c) correlating the expression of said transcript to one ormore embryonic stages.
 3. The method of claim 2 wherein at least two RNAtranscripts are detected or determined and correlated to one or moreembryonic stages.
 4. The method of claim 2 wherein expression of the atleast one RNA transcript is analyzed by hybridization with at least oneprobe of Table I.
 5. The method of claim 2 wherein expression of the atleast one RNA transcript is analyzed by hybridization with a variant ofat least one probe of Table I.
 6. The method of claim 5 wherein saidvariant hybridizes to at least one probe of Table I under conditions ofhigh stringency.
 7. The method of claim 5 wherein said varianthybridizes to at least one probe of Table I under conditions of moderatestringency.
 8. The method of claim 2 wherein expression of at least oneRNA transcript is detected or determined by at least one member of thegroup consisting of PCR, Northern Analysis, and in situ hybridization.9. The method of claim 2 wherein expression of said at least two RNAtranscripts are detected by a DNA array.
 10. A database comprising amultiplicity of nucleotide sequences shown in any one of Table I,including variants thereof, wherein said variants hybridize underconditions of high stringency to either strand of a denatured,double-stranded DNA comprising any of SEQ ID NOS: 1-327.
 11. Thedatabase of claim 10 wherein said variants hybridize under conditions ofmoderate stringency.
 12. A DNA array comprising a multiplicity ofnucleotide sequences shown in Table I, including variants thereof,wherein said variants hybridize under conditions of high stringency toeither strand of a denatured, double-stranded DNA comprising any of SEQID NOS: 1-327.
 13. The DNA array of claim 12 wherein said variantshybridize under conditions of moderate stringency.
 14. A method forstaging plant embryos comprising: a) selecting total RNA from amultiplicity of embryos of known developmental age; b) correlating theembryonic expression pattern to the developmental age to develop arelational database; c) determining levels of expression from embryos ofunknown developmental age by hybridization to a DNA array comprising amultiplicity of the nucleotide sequences shown in any one of SEQ ID NOS:1-327; d) correlating the expression pattern from step 3 to therelational database to determine developmental stage for the unknownembryo.
 15. The method of claim 14 wherein the embryos of step 1) arezygotic embryos.
 16. The method of claim 14 further comprising the stepof altering the embryonic growth conditions to approximate theexpression pattern of zygotic embryos.
 17. An isolated nucleic acidvariant of the nucleotide sequence shown in any one of SEQ ID NOS:1-334, wherein said variant hybridizes under conditions of moderatestringency to either strand of a denatured, double-stranded DNAcomprising any of SEQ ID NOS: 1-334.
 18. An isolated polypeptide encodedby a nucleic acid molecule of claim
 17. 19. An isolated nucleic acidencoding the polypeptide of claim
 18. 20. Antibodies that specificallybind to the peptide of claim
 18. 21. The antibodies of claim 20, whereinsaid antibodies are monoclonal.
 22. A recombinant vector that directsthe expression of a nucleic acid of claim
 17. 23. A host celltransformed with the vector of claim
 22. 24. The host cell of claim 23,wherein the host is a somatic pine embryo.
 25. A method for stagingplant embryos comprising: a) selecting total RNA from at least oneembryo of known developmental age; b) determining the level ofexpression of a multiplicity of genes which hybridize to one or more ofSEQ ID NOS: 1-327; c) correlating the known developmental ages of theembryos from step 1) with the profile of expression measured in step 2);d) applying the correlation of step 3) to a sample of embryo RNA fromembryos to be staged; and e) determining the embryo stage.
 26. Themethod of claim 25, wherein the measurement of gene expression is byRT-PCR.
 27. The method of claim 25, wherein the measurement of geneexpression is by nucleic acid hybridization.
 28. The method of claim 25,wherein the measurement of gene expression is by determining the levelof protein expression.
 29. The method of claim 28, wherein proteinexpression is measured by antibody binding.
 30. A method for selectingadvantageous plant clones comprising: a) selecting one or more samplesof embryonic RNA from multiple clones of plants; b) determining that atleast one sampled clone has an advantageous characteristic; c) comparingthe embryonic levels of expression of genes which hybridize to one ormore of SEQ ID NOS: 1-327 in samples from the advantageous clone withexpression levels in at least one clone that does not show theadvantageous characteristic; and d) selecting additional clones whichshow an embryonic gene expression pattern more similar to that of theadvantageous clone than to the pattern of at least one clone that doesnot show the advantageous characteristic.
 31. Method of claim 30 wherethe clones to be sampled or compared are from about the samedevelopmental age.
 32. Method of claim 31 where the development age isvisually detected.
 33. The method of claim 30, wherein the measurementof gene expression is by RT-PCR.
 34. The method of claim 30, wherein themeasurement of gene expression is by nucleic acid hybridization.
 35. Themethod of claim 30, wherein the measurement of gene expression is bydetermining the level of protein expression.
 36. The method of claim 35,wherein protein expression is measured by antibody binding.
 37. A methodof determining embryo fitness comprising: a) creating a relationaldatabase with RNA expression values for genes listed in Table I forembryos of known developmental stages; b) isolating total RNA fromembryos of unknown stage development; c) measuring expression levels ofgenes identified in Table I from the solated total RNA; and d)correlating the database of step 1) with the pattern of expressiondetermined in steps 2) and 3) to assess proper embryo development. 38.The method of claim 37, wherein the measurement of gene expression is byRT-PCR.
 39. The method of claim 37, wherein the measurement of geneexpression is by nucleic acid hybridization.
 40. The method of claim 37,wherein the measurement of gene expression is by determining the levelof protein expression.
 41. The method of claim 40, wherein proteinexpression is measured by antibody binding.
 42. A method for selectingadvantageous growth conditions for embryo development comprising: a)determining RNA expression profiles for staged embryos under controlculture conditions; b) altering culture conditions; c) determining RNAexpression profiles for staged embryos under altered culture conditions;and d) correlating culture change to developmental effect in embryo. 43.The method of claim 42, wherein conditions are selected which produceRNA expression profiles most closely approximating late-stage embryoprofiles.
 44. The method of claim 42, wherein the culture conditions arealtered by operatively linking one or more stage-specific embryopromoter(s) to one or more sense or antisense nucleic acid molecules.45. The method of claim 42, wherein the culture conditions are alteredby operatively linking one more stage-specific embryo promoter(s)selected from SEQ ID NOS: 328-334 to one or more sense or antisensenucleic acid molecules.
 46. The method of claim 42, wherein the changein expression profiles is correlated by a relational database.
 47. Arecombinant nucleic acid molecule encoding a product during embryodevelopment comprising: a) a first nucleic acid sequence which is theLP2-3 promoter; and b) a second nucleic acid sequence encoding aproduct, wherein the first nucleic acid is operatively linked to thesecond nucleic acid molecule whereby its expression is directed by thepromoter sequence.
 48. The recombinant nucleic acid molecule of claim 47wherein the second nucleic acid sequence encodes for GFP, or a variantof GFP.
 49. The recombinant nucleic acid molecule of claim 48 whereinthe second nucleic acid sequence is linked to one or more additionalnucleic acid molecules.
 50. The recombinant nucleic acid molecule ofclaim 49 wherein the additional molecule encodes a protein productnormally expressed, by a developing embryo at a known stage.
 51. Therecombinant nucleic acid molecule of claim 47 wherein the second nucleicacid sequence encodes an embryo-derived molecule.
 52. The recombinantnucleic acid molecule of claim 51 embryo-derived molecule isstage-specific.
 53. A plant cell comprising the recombinant nucleic acidmolecule of claim
 47. 54. A method for producing a protein productduring embryo development comprising: a) operatively linking one morestage-specific embryo promoter(s) to one or more nucleic acid moleculesthat encode a protein product, b) delivering construct to developingembryos.
 55. The method of claim 54 wherein the operatively linkednucleic acid molecule is a reporter or indicator gene.
 56. The method ofclaim 54 wherein the operatively linked nucleic acid molecule is GFP, ora variant of GFP
 57. The method of claim 54 wherein at least onestage-specific promoter is selected from SEQ ID NOS: 328-334.
 58. Amethod for staging embryos comprising: a) providing one or morestage-specific embryo promoter(s) operatively linked to one or morenucleic acid molecules that encode a protein product to developingembryos, b) monitoring expression of the protein product as the embryomatures through stage in which promoter functions.
 59. The method ofclaim 58 wherein the operatively linked nucleic acid molecule is areporter or indicator gene.
 60. The method of claim 58 wherein theoperatively linked nucleic acid molecule is GFP, or a variant of GFP.61. The method of claim 58 wherein at least one stage-specific promoteris selected from SEQ ID NOS: 328-334.